Insight into the stereospecificity of short-chain Thermus thermophilus alcohol dehydrogenase showing pro-S hydride transfer and Prelog enantioselectivity
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Insight into the stereospecificity of short-chain Thermus thermophilus alcohol dehydrogenase showing pro-S hydride transfer and Prelog enantioselectivity (750 views)

Pennacchio A, Giordano A, Esposito L, Langella E, Rossi M, Raia CA

Protein Pept Lett (ISSN: 0929-8665, 1875-5305), 2010 Apr; 17(4): 437-443.

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Paper type: Journal Article,

Impact factor: 1.849, 5-year impact factor: 1.45

Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-77949957731&partnerID=40&md5=4f6b4e76221e675ce349d72852226469

Keywords: Bacillus Stearothermophilus, Cofactor Stereospecificity, Enantioselectivity, Prelog Rule, Short-Chain Dehydrogenase Reductase, Thermus Thermophilus, 2 Propanol, Alcohol Dehydrogenase, Bacterial Protein, Deuterium, Nicotinamide Adenine Dinucleotide, Article, Chemical Structure, Chemistry, Enzymology, Metabolism, Nuclear Magnetic Resonance, Protein Conformation, Stereoisomerism, 2-Propanol, Models, Molecular, Biomolecular, Geobacillus Stearothermophilus,

Affiliations:
*** IBB - CNR ***

Istituto di Biochimica delle Proteine, CNR, Via P. Castellino 111, I-80131, NA, Italy
Istituto di Chimica Biomolecolare, CNR, Comprensorio Olivetti, Edificio 70, Via Campi Flegrei 34, I-80078 Pozzuoli (NA), Italy
Istituto di Biostrutture e Bioimmagini, CNR, Via Mezzocannone 16, I-80134, NA, Italy


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Insight into the stereospecificity of short-chain Thermus thermophilus alcohol dehydrogenase showing pro-S hydride transfer and Prelog enantioselectivity

The stereochemistry of the hydride transfer in reactions catalyzed by NAD(H)-dependent alcohol dehydrogenase from Thermus thermophilus HB27 was determined by means of 1H-NMR spectroscopy. The enzyme transfers the pro-S hydrogen of [4R-2H]NADH and exhibits Prelog specificity. Enzyme-substrate docking calculations provided structural details about the enantioselectivity of this thermophilic enzyme. These results give additional insights into the diverse active site architectures of the largely versatile short-chain dehydrogenase superfamily enzymes. A feasible protocol for the synthesis of [4R-2H]NADH with high yield was also set up by enzymatic oxidation of 2-propanol-d8 catalyzed by Bacillus stearothermophilus alcohol dehydrogenase. © 2010 Bentham Science Publishers Ltd.
Insight into the stereospecificity of short-chain Thermus thermophilus alcohol dehydrogenase showing pro-S hydride transfer and Prelog enantioselectivity

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Insight into the stereospecificity of short-chain Thermus thermophilus alcohol dehydrogenase showing pro-S hydride transfer and Prelog enantioselectivity

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5 Records (5 excluding Abstracts).
Total impact factor: 19.641 (19.641 excluding Abstracts).
Total 5 year impact factor: 20.125 (20.125 excluding Abstracts).

Last modified by Marco Comerci on Sunday 12 July 2020, 13:15:00
750 views. Last view on Thursday 12 December 2024, 17:27:40