A targeted secretome profiling by multiplexed immunoassay revealed that secreted chemokine ligand 2 (MCP-1/CCL2) affects neural differentiation in mesencephalic neural progenitor cells (948 views)
Proteomics (ISSN: 1615-9853), 2015 Feb; 15(4): 714-724.
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Paper type: Journal Article,
Impact factor: 4.079, 5-year impact factor: 3.666
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84922950095&partnerID=40&md5=ef8c1e7e53d97b3dc4f4b1b95aa8f36b
Keywords: Ccl2, Cell Biology, Cytokine Profiling, Multiplexed Immunoassay, Neural Differentiation, Secretome, Gamma Interferon, Interleukin 10, Interleukin 12p70, Interleukin 1alpha, Interleukin 1beta, Monocyte Chemotactic Protein 1, Myc Protein, Rantes, Tumor Necrosis Factor Alpha, Animal Cell, Animal Tissue, Article, Cell Fate, Cell Proliferation, Cell Viability Assay, Culture Medium, Cytokine Release, Embryo, Mesencephalon, Mouse, Nerve Cell Culture, Nerve Cell Differentiation, Neural Stem Cell, Neuroblast, Nonhuman, Phenotype, Pluripotent Stem Cell, Primary Culture, Priority Journal, Protein Expression, Signal Transduction, Tissue Culture,
Affiliations:
*** IBB - CNR ***
Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of NaplesCaserta, Italy
CIRN, Inter-University Center for Research in NeuroscienceNapoli, Italy
Department of Pharmacy, University of Naples Federico IINaples, Italy
Institute of Genetics and Biophysics Adriano Buzzati Traverso, CNRNaples, Italy
Institute of Biostructures and Bioimaging - IBB, CNR and CIRPeBNapoli, Italy
Department of Translational Medical Sciences, Federico II University of NaplesNapoli, Italy
IRCCSMultimedica, Milano, Italy
IRCCS, Multimedica, Milano, Italy
References:
Not available.
Proteomics (ISSN: 1615-9853), 2015 Feb; 15(4): 714-724.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 4.079, 5-year impact factor: 3.666
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84922950095&partnerID=40&md5=ef8c1e7e53d97b3dc4f4b1b95aa8f36b
Keywords: Ccl2, Cell Biology, Cytokine Profiling, Multiplexed Immunoassay, Neural Differentiation, Secretome, Gamma Interferon, Interleukin 10, Interleukin 12p70, Interleukin 1alpha, Interleukin 1beta, Monocyte Chemotactic Protein 1, Myc Protein, Rantes, Tumor Necrosis Factor Alpha, Animal Cell, Animal Tissue, Article, Cell Fate, Cell Proliferation, Cell Viability Assay, Culture Medium, Cytokine Release, Embryo, Mesencephalon, Mouse, Nerve Cell Culture, Nerve Cell Differentiation, Neural Stem Cell, Neuroblast, Nonhuman, Phenotype, Pluripotent Stem Cell, Primary Culture, Priority Journal, Protein Expression, Signal Transduction, Tissue Culture,
Affiliations:
*** IBB - CNR ***
Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of NaplesCaserta, Italy
CIRN, Inter-University Center for Research in NeuroscienceNapoli, Italy
Department of Pharmacy, University of Naples Federico IINaples, Italy
Institute of Genetics and Biophysics Adriano Buzzati Traverso, CNRNaples, Italy
Institute of Biostructures and Bioimaging - IBB, CNR and CIRPeBNapoli, Italy
Department of Translational Medical Sciences, Federico II University of NaplesNapoli, Italy
IRCCSMultimedica, Milano, Italy
IRCCS, Multimedica, Milano, Italy
References:
Not available.
A targeted secretome profiling by multiplexed immunoassay revealed that secreted chemokine ligand 2 (MCP-1/CCL2) affects neural differentiation in mesencephalic neural progenitor cells
Chemokines and cytokines, primarily known for their roles in the immune and inflammatory response, have also been identified as key components of the neurogenic niche where they are involved in the modulation of neural stem cell proliferation and differentiation. However, a complete understanding of the functional role played in neural differentiation and a comprehensive profiling of these secreted molecules are lacking. By exploiting the multiplexing capability of magnetic bead-based immunoassays, we have investigated the changes of the expression levels of a set of chemokines and cytokines released from the pluripotent neural cell line mes-c-myc A1 following its differentiation from a proliferating phenotype (A1P) toward a neural (A1D) phenotype. We found a subset of molecules exclusively released from A1P, whereas others were differentially detected in A1P and A1D conditioned media. Among them, we identified monocyte chemoattractant protein-1/chemokine ligand 2 (MCP-1/CCL2) as a proneurogenic factor able to affect neuronal differentiation of A1 cells as well as of neuroblasts from primary cultures and to induce the elongation and/or formation of neuritic processes. Altogether, data are suggestive of a main role played by the CCL2/CCR2 signaling pathway and in general of the network of secreted cytokines/chemokines in the differentiation of neural progenitor cells toward a neural fate. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Chemokines and cytokines, primarily known for their roles in the immune and inflammatory response, have also been identified as key components of the neurogenic niche where they are involved in the modulation of neural stem cell proliferation and differentiation. However, a complete understanding of the functional role played in neural differentiation and a comprehensive profiling of these secreted molecules are lacking. By exploiting the multiplexing capability of magnetic bead-based immunoassays, we have investigated the changes of the expression levels of a set of chemokines and cytokines released from the pluripotent neural cell line mes-c-myc A1 following its differentiation from a proliferating phenotype (A1P) toward a neural (A1D) phenotype. We found a subset of molecules exclusively released from A1P, whereas others were differentially detected in A1P and A1D conditioned media. Among them, we identified monocyte chemoattractant protein-1/chemokine ligand 2 (MCP-1/CCL2) as a proneurogenic factor able to affect neuronal differentiation of A1 cells as well as of neuroblasts from primary cultures and to induce the elongation and/or formation of neuritic processes. Altogether, data are suggestive of a main role played by the CCL2/CCR2 signaling pathway and in general of the network of secreted cytokines/chemokines in the differentiation of neural progenitor cells toward a neural fate. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
A targeted secretome profiling by multiplexed immunoassay revealed that secreted chemokine ligand 2 (MCP-1/CCL2) affects neural differentiation in mesencephalic neural progenitor cells
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A targeted secretome profiling by multiplexed immunoassay revealed that secreted chemokine ligand 2 (MCP-1/CCL2) affects neural differentiation in mesencephalic neural progenitor cells
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13 Records (12 excluding Abstracts).
Total impact factor: 83.732 (83.732 excluding Abstracts).
Total 5 year impact factor: 83.373 (83.373 excluding Abstracts).
Total impact factor: 83.732 (83.732 excluding Abstracts).
Total 5 year impact factor: 83.373 (83.373 excluding Abstracts).
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