Characterization of Human Mesenchymal Stem Cells Isolated from the Testis(380 views) De Chiara L, Famulari ES, Fagoonee S, Van Daalen SKM, Buttiglieri S, Revelli A, Tolosano E, Silengo L, Van Pelt AMM, Altruda F
Centro di Eccellenza DeNothe, Department of Biomedical, Experimental and Clinical Sciences, University of Florence, Viale Pieraccini 6, 50139 Firenze, Italy.
Molecular Biotechnology Center, Department of Molecular Biotechnology and Health Sciences, University of Turin, Via Nizza 52, 10126 Turin, Italy.
The Institute of Biostructure and Bioimaging (CNR) c/o Molecular Biotechnology Center, Turin, Italy.
Center for Reproductive Medicine, Women's and Children's Hospital, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, Netherlands.
Obstetrics and Gynecology 1U, Physiopathology of Reproduction and IVF Unit, Department of Surgical Sciences, Sant'Anna Hospital, University of Turin, Corso Spezia 60, 10126 Turin, Italy.
References: Not available.
Characterization of Human Mesenchymal Stem Cells Isolated from the Testis
Mesenchymal stem cells hold great promise for regenerative medicine as they can be easily isolated from different sources such as adipose tissue, bone marrow, and umbilical cord blood. Spontaneously arising pluripotent stem cells can be obtained in culture from murine spermatogonial stem cells (SSCs), while the pluripotency of the human counterpart remains a matter of debate. Recent gene expression profiling studies have demonstrated that embryonic stem cell- (ESC-) like cells obtained from the human testis are indeed closer to mesenchymal stem cells (MSCs) than to pluripotent stem cells. Here, we confirm that colonies derived from human testicular cultures, with our isolation protocol, are of mesenchymal origin and do not arise from spermatogonial stem cells (SSCs). The testis, thus, provides an important and accessible source of MSCs (tMSCs) that can be potentially used for nephrotoxicity testing in vitro. We further demonstrate, for the first time, that tMSCs are able to secrete microvesicles that could possibly be applied to the treatment of various chronic diseases, such as those affecting the kidney.
Characterization of Human Mesenchymal Stem Cells Isolated from the Testis
Malvindi MA, Greco A, Conversano F, Figuerola A, Corti M, Bonora M, Lascialfari A, Doumari HA, Moscardini M, Cingolani R, Gigli G, Casciaro S, Pellegrino T, Ragusa A * MR Contrast Agents(431 views) Small Animal Imaging, 2011 Jul 8; 21(13): 2548-2555. Impact Factor:1.784 ViewExport to BibTeXExport to EndNote