Persistent cystic fibrosis isolate Pseudomonas aeruginosa strain RP73 exhibits an under-acylated LPS structure responsible of its low inflammatory activity (367 views)
Mol Immunol (ISSN: 0161-5890), 2015; 63(2): 166-175.
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Paper type: Journal Article,
Impact factor: 3.375, 5-year impact factor: 2.966
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84912010967&partnerID=40&md5=ae91c99ad38727b86862699539c97f93
Keywords: Chronic Infections In Cystic Fibrosis, Lipid A, Lipopolysaccharide, Pseudomonas Aeruginosa, Structural Analysis, Deoxyribonuclease, Lipooligosaccharide, Proteinase, Lipid-Linked Oligosaccharides, Peroxidase, Article, Bacterium Isolation, Biological Activity, Carbon Nuclear Magnetic Resonance, Controlled Study, Drug Structure, Entamoeba Coli, Freeze Drying, Heteronuclear Multiple Bond Correlation, Heteronuclear Single Quantum Coherence, Human, Hydrolysis, Immune Response, Inflammatory Activity, Innate Immunity, Lung Lavage, Mass Fragmentography, Matrix Assisted Laser Desorption Ionization Time Of Flight Mass Spectrometry, Nonhuman, Nuclear Magnetic Resonance Spectroscopy, Nuclear Overhauser Effect, Polyacrylamide Gel Electrophoresis, Proton Nuclear Magnetic Resonance, Silver Staining, Acylation, Animal, C57bl Mouse, Chemistry, Immunology, Inflammation, Isolation And Purification, Metabolism, Microbiology, Neutrophil Chemotaxis, Pathology, Physiology, Carbon-13 Magnetic Resonance Spectroscopy, Inbred C57bl, Neutrophil Infiltration, Proton Magnetic Resonance Spectroscopy, Matrix-Assisted Laser Desorption-Ionization,
Affiliations:
*** IBB - CNR ***
Dipartimento di Scienze Chimiche, Università di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, Napoli, Italy
Infection and Cystic Fibrosis Unit, San Raffaele Scientific Institute, Milano, Italy
Istituto di Chimica e Tecnologia dei Polimeri - ICTP - CNR, Via P. Gaifami 18, Catania, Italy
References:
Alexander, C., Rietschel, E.T., Bacterial lipopolysaccharides and innate immunity (2001) J. Endotoxin Res., 7, pp. 167-20
Birnbaum, G.I., Conformations of ammonium 3-deoxy-d-manno-2-octulosonate (KDO) and methyl α- and β-ketopyranosides of KDO: X-ray structure and 1H NMR analyses (1987) J. Carbohydr. Chem., 6, pp. 17-39
Bock, K., Isolation and structural analysis of oligosaccharide phosphates containing the complete carbohydrate chain of the lipopolysaccharide from Vibrio cholerae strain H11 (non-O1) (1994) Eur. J. Biochem., 225, pp. 1029-1039
Bragonzi, A., Sequence diversity of the mucABD locus in Pseudomonas aeruginosa isolates from patients with cystic fibrosis (2006) Microbiology, 152, pp. 3261-3269
Bragonzi, A., Pseudomonas aeruginosa microevolution during cystic fibrosis lung infection establishes clones with adapted virulence (2009) AJRCCM, 180, pp. 138-145
Bystrova, O.V., Structural studies on the core and the O-polysaccharide repeating unit of Pseudomonas aeruginosa immunotype 1 lipopolysaccharide (2002) Eur. J. Biochem., 269, pp. 2194-2203
Bystrova, O.V., Full structure of the lipopolysaccharide of Pseudomonas aeruginosa immunotype 5 (2004) Biochemistry (Mosc.), 69 (2), pp. 170-175
Caroff, M., Detergent-accelerated hydrolysis of bacterial endotoxins and determination of the anomeric configuration of the glycosyl phosphate present in the isolated lipid A fragment of the Bordetella pertussis endotoxin (1988) Carbohydr. Res., 175, pp. 273-282
Cigana, C., Pseudomonas aeruginosa exploits lipid A and muropeptides modification as a strategy to lower innate immunity during cystic fibrosis lung infection (2009) PLoS ONE, 4 (12), p. e8439
Ciucanu, I., Kerek, F., A simple and rapid method for the permethylation of carbohydrates (1984) Carbohydr. Res., 131, pp. 209-217
Dean, C.R., WbjA adds glucose to complete the O-antigen trisaccharide repeating unit of the lipopolysaccharide of Pseudomonas aeruginosa serogroup O11 (2002) J. Bacteriol., 184 (1), pp. 323-326
De Castro, C., Microbe-associated molecular patterns in innate immunity: extraction and chemical analysis of Gram-negative bacterial lipopolysaccharides (2010) Methods Enzymol., 480, pp. 89-115
De Soyza, A., Lipopolysaccharide structure and biological activity from the cystic fibrosis pathogens Burkholderia cepacia complex (2012) Carbohydr. Chem., 38, pp. 13-39
Döring, G., Immunologic aspects of cystic fibrosis (1988) Chest, 94, pp. 109S-115S
Ernst, R.K., Specific lipopolysaccharide found in cystic fibrosis airway Pseudomonas aeruginosa (1999) Science, 286, p. 1561
Ernst, R.K., Pseudomonas aeruginosa lipid A diversity and its recognition by Toll-like receptor 4 (2003) J. Endotoxin Res., 9, pp. 395-400
Galanos, C., A new method for the extraction of R lipopolysaccharides (1969) Eur. J. Biochem., 9, pp. 245-249
Gibson, R.L., Pathophysiology and management of pulmonary infections in cystic fibrosis (2003) Am. J. Respir. Crit. Care Med., 168, pp. 918-951
Hajjar, A.M., Human Toll-like receptor 4 recognizes host-specific LPS modifications (2002) Nat. Immunol., 3, pp. 354-359
Holst, O., Preparation and structural analysis of oligosaccharide monophosphates obtained from the lipopolysaccharide of recombinant strains of Salmonella minnesota and Escherichia coli expressing the genus-specific epitope of Chlamydia lipopolysaccharide (1994) Eur. J. Biochem., 222, pp. 183-194
Holst, O., The structures of oligosaccharide bisphosphates isolated from the lipopolysaccharide of a recombinant Escherichia coli strain expressing the gene gseA [3-deoxy-d-manno-octulopyranosonic acid (Kdo) transferase of Chlamydia psittaci 6BC (1995) Eur. J. Biochem., 229, pp. 194-200
Holst, O., (1999) Endotoxin in Health and Disease, pp. 115-154. , Marcel Dekker, New York, H. Brade, D.C. Morrsion, S. Opal, S. Vogel (Eds.)
Holst, O., Deacylation of lipopolysaccharides and isolation of oligosaccharide phosphates (2000) Methods Mol. Biol., 145, pp. 345-353
Holst, O., Chemical structure of the core region of lipopolysaccharides. An update (2002) Trends Glycosci. Glycotechnol., 14, pp. 87-103
Jeukens, J., Complete genome sequence of persistent Cystic Fibrosis isolate Pseudomonas aeruginosa strain RP73 (2013) Genome Announc., 1 (4). , e00568-13
Kittelberger, R., Hilbink, F.J., Sensitive silver-staining detection of bacterial lipopolysaccharides in polyacrylamide gels (1993) Biochem. Biophys. Methods, 26, pp. 81-86
Knirel, Y.A., Structural analysis of the lipopolysaccharide core of a rough, cystic fibrosis isolate of Pseudomonas aeruginosa (2001) Eur. J. Biochem., 268 (17), pp. 4708-4719
Kocíncová, D., Lam, J.S., Structural diversity of the core oligosaccharide domain of Pseudomonas aeruginosa lipopolysaccharide (2011) Biochemistry (Mosc.), 76 (7), pp. 755-760
Kooistra, O., Structure of a highly phosphorylated lipopolysaccharide core in the Delta algC mutants derived from Pseudomonas aeruginosa wild-type strains PAO1 (serogroup O5) and PAC1R (serogroup O3) (2003) Carbohydr. Res., 338 (23), pp. 2667-2677
Kresse, A.U., Impact of large chromosomal inversions on the adaptation and evolution of Pseudomonas aeruginosa chronically colonizing cystic fibrosis lungs (2003) Mol. Microbiol., 47, pp. 145-158
Leontein, K., Lönngren, J., Determination of the absolute configuration of sugars by gas-liquid chromatography of their acetylated 2-octyl glycosides (1978) Methods Carbohydr. Chem., 62, pp. 359-362
Masoud, H., Structural elucidation of the lipopolysaccharide core region of the O-chain-deficient mutant strain A28 from Pseudomonas aeruginosa serotype 06 (International Antigenic Typing Scheme) (1995) J. Bacteriol., 177 (23), pp. 6718-6726
Moalli, F., The therapeutic potential of the humoral pattern recognition molecule PTX3 in chronic lung infection caused by Pseudomonas aeruginosa (2011) J. Immunol., 186, pp. 5425-5434
Paroni, M., Response of CFTR-deficient mice to long-term Pseudomonas aeruginosa chronic infection and PTX3 therapy (2013) J. Infect. Dis., 208, pp. 130-138
Piantini, U., Multiple quantum filters for elucidating NMR coupling networks (1982) J. Am. Chem. Soc., 104, pp. 6800-6801
Pier, G.B., Promises and pitfalls of Pseudomonas aeruginosa lipopolysaccharide as a vaccine antigen (2003) Carbohydr. Res., 338, pp. 2549-2556
Rance, M., Improved spectral resolution in cosy 1H NMR spectra of proteins via double quantum filtering (1983) Biochem. Biophys. Res. Commun., 117, pp. 479-485
Rowe, P.S., Meadow, P.M., Structure of the core oligosaccharide from the lipopolysaccharide of Pseudomonas aeruginosa PAC1R and its defective mutants (1983) Eur. J. Biochem., 132 (2), pp. 329-337
Sadovskaya, I., Structural elucidation of the lipopolysaccharide core regions of the wild-type strain PAO1 and O-chain-deficient mutant strains AK1401 and AK1012 from Pseudomonas aeruginosa serotype O5 (1998) Eur. J. Biochem., 255 (3), pp. 673-684
Sánchez Carballo, P.M., Elucidation of the structure of an alanine-lacking core tetrasaccharide trisphosphate from the lipopolysaccharide of Pseudomonas aeruginosa mutant H4 (1999) Eur. J. Biochem., 261 (2), pp. 500-508
Silipo, A., Ammonium hydroxide hydrolysis: a valuable support in the MALDI-TOF mass spectrometry analysis of Lipid A fatty acid distribution (2002) J. Lipid Res., 43, pp. 2188-2195
Silipo, A., The structure of the phosphorylated carbohydrate backbone of the lipopolysaccharide of the phytopathogen bacterium Pseudomonas tolaasii (2004) Carbohydr. Res., 339, pp. 2241-2248
Silipo, A., Structure elucidation of the highly heterogeneous lipid A from the lipopolysaccharide of the Gram-negative extremophile bacterium Halomonas magadiensis strain 21 M1 (2004) Eur. J. Org. Chem., 10, pp. 2263-2271
Silipo, A., Full structural characterisation of the lipooligosaccharide of a Burkholderia pyrrocinia clinical isolate (2006) Eur. J. Org. Chem., 21, pp. 4874-4883
Smith, E.E., Genetic adaptation by Pseudomonas aeruginosa to the airways of cystic fibrosis patients (2006) Proc. Natl. Acad. Sci. U.S.A., 103, pp. 8487-8492
States, D.J., A two-dimensional nuclear Overhauser experiment with pure absorption phase in four quadrants (1982) J. Magn. Reson., 48, pp. 286-292
Stover, C.K., Complete genome sequence of Pseudomonas aeruginosa PA01, an opportunistic pathogen (2000) Nature, 406, pp. 959-964
Sturiale, L., Reflectron MALDI TOF and MALDI TOF/TOF mass spectrometry reveal novel structural details of native lipooligosaccharides (2011) J. Mass Spectrom., 46, pp. 1135-1142
Mol Immunol (ISSN: 0161-5890), 2015; 63(2): 166-175.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 3.375, 5-year impact factor: 2.966
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84912010967&partnerID=40&md5=ae91c99ad38727b86862699539c97f93
Keywords: Chronic Infections In Cystic Fibrosis, Lipid A, Lipopolysaccharide, Pseudomonas Aeruginosa, Structural Analysis, Deoxyribonuclease, Lipooligosaccharide, Proteinase, Lipid-Linked Oligosaccharides, Peroxidase, Article, Bacterium Isolation, Biological Activity, Carbon Nuclear Magnetic Resonance, Controlled Study, Drug Structure, Entamoeba Coli, Freeze Drying, Heteronuclear Multiple Bond Correlation, Heteronuclear Single Quantum Coherence, Human, Hydrolysis, Immune Response, Inflammatory Activity, Innate Immunity, Lung Lavage, Mass Fragmentography, Matrix Assisted Laser Desorption Ionization Time Of Flight Mass Spectrometry, Nonhuman, Nuclear Magnetic Resonance Spectroscopy, Nuclear Overhauser Effect, Polyacrylamide Gel Electrophoresis, Proton Nuclear Magnetic Resonance, Silver Staining, Acylation, Animal, C57bl Mouse, Chemistry, Immunology, Inflammation, Isolation And Purification, Metabolism, Microbiology, Neutrophil Chemotaxis, Pathology, Physiology, Carbon-13 Magnetic Resonance Spectroscopy, Inbred C57bl, Neutrophil Infiltration, Proton Magnetic Resonance Spectroscopy, Matrix-Assisted Laser Desorption-Ionization,
Affiliations:
*** IBB - CNR ***
Dipartimento di Scienze Chimiche, Università di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, Napoli, Italy
Infection and Cystic Fibrosis Unit, San Raffaele Scientific Institute, Milano, Italy
Istituto di Chimica e Tecnologia dei Polimeri - ICTP - CNR, Via P. Gaifami 18, Catania, Italy
References:
Alexander, C., Rietschel, E.T., Bacterial lipopolysaccharides and innate immunity (2001) J. Endotoxin Res., 7, pp. 167-20
Birnbaum, G.I., Conformations of ammonium 3-deoxy-d-manno-2-octulosonate (KDO) and methyl α- and β-ketopyranosides of KDO: X-ray structure and 1H NMR analyses (1987) J. Carbohydr. Chem., 6, pp. 17-39
Bock, K., Isolation and structural analysis of oligosaccharide phosphates containing the complete carbohydrate chain of the lipopolysaccharide from Vibrio cholerae strain H11 (non-O1) (1994) Eur. J. Biochem., 225, pp. 1029-1039
Bragonzi, A., Sequence diversity of the mucABD locus in Pseudomonas aeruginosa isolates from patients with cystic fibrosis (2006) Microbiology, 152, pp. 3261-3269
Bragonzi, A., Pseudomonas aeruginosa microevolution during cystic fibrosis lung infection establishes clones with adapted virulence (2009) AJRCCM, 180, pp. 138-145
Bystrova, O.V., Structural studies on the core and the O-polysaccharide repeating unit of Pseudomonas aeruginosa immunotype 1 lipopolysaccharide (2002) Eur. J. Biochem., 269, pp. 2194-2203
Bystrova, O.V., Full structure of the lipopolysaccharide of Pseudomonas aeruginosa immunotype 5 (2004) Biochemistry (Mosc.), 69 (2), pp. 170-175
Caroff, M., Detergent-accelerated hydrolysis of bacterial endotoxins and determination of the anomeric configuration of the glycosyl phosphate present in the isolated lipid A fragment of the Bordetella pertussis endotoxin (1988) Carbohydr. Res., 175, pp. 273-282
Cigana, C., Pseudomonas aeruginosa exploits lipid A and muropeptides modification as a strategy to lower innate immunity during cystic fibrosis lung infection (2009) PLoS ONE, 4 (12), p. e8439
Ciucanu, I., Kerek, F., A simple and rapid method for the permethylation of carbohydrates (1984) Carbohydr. Res., 131, pp. 209-217
Dean, C.R., WbjA adds glucose to complete the O-antigen trisaccharide repeating unit of the lipopolysaccharide of Pseudomonas aeruginosa serogroup O11 (2002) J. Bacteriol., 184 (1), pp. 323-326
De Castro, C., Microbe-associated molecular patterns in innate immunity: extraction and chemical analysis of Gram-negative bacterial lipopolysaccharides (2010) Methods Enzymol., 480, pp. 89-115
De Soyza, A., Lipopolysaccharide structure and biological activity from the cystic fibrosis pathogens Burkholderia cepacia complex (2012) Carbohydr. Chem., 38, pp. 13-39
Döring, G., Immunologic aspects of cystic fibrosis (1988) Chest, 94, pp. 109S-115S
Ernst, R.K., Specific lipopolysaccharide found in cystic fibrosis airway Pseudomonas aeruginosa (1999) Science, 286, p. 1561
Ernst, R.K., Pseudomonas aeruginosa lipid A diversity and its recognition by Toll-like receptor 4 (2003) J. Endotoxin Res., 9, pp. 395-400
Galanos, C., A new method for the extraction of R lipopolysaccharides (1969) Eur. J. Biochem., 9, pp. 245-249
Gibson, R.L., Pathophysiology and management of pulmonary infections in cystic fibrosis (2003) Am. J. Respir. Crit. Care Med., 168, pp. 918-951
Hajjar, A.M., Human Toll-like receptor 4 recognizes host-specific LPS modifications (2002) Nat. Immunol., 3, pp. 354-359
Holst, O., Preparation and structural analysis of oligosaccharide monophosphates obtained from the lipopolysaccharide of recombinant strains of Salmonella minnesota and Escherichia coli expressing the genus-specific epitope of Chlamydia lipopolysaccharide (1994) Eur. J. Biochem., 222, pp. 183-194
Holst, O., The structures of oligosaccharide bisphosphates isolated from the lipopolysaccharide of a recombinant Escherichia coli strain expressing the gene gseA [3-deoxy-d-manno-octulopyranosonic acid (Kdo) transferase of Chlamydia psittaci 6BC (1995) Eur. J. Biochem., 229, pp. 194-200
Holst, O., (1999) Endotoxin in Health and Disease, pp. 115-154. , Marcel Dekker, New York, H. Brade, D.C. Morrsion, S. Opal, S. Vogel (Eds.)
Holst, O., Deacylation of lipopolysaccharides and isolation of oligosaccharide phosphates (2000) Methods Mol. Biol., 145, pp. 345-353
Holst, O., Chemical structure of the core region of lipopolysaccharides. An update (2002) Trends Glycosci. Glycotechnol., 14, pp. 87-103
Jeukens, J., Complete genome sequence of persistent Cystic Fibrosis isolate Pseudomonas aeruginosa strain RP73 (2013) Genome Announc., 1 (4). , e00568-13
Kittelberger, R., Hilbink, F.J., Sensitive silver-staining detection of bacterial lipopolysaccharides in polyacrylamide gels (1993) Biochem. Biophys. Methods, 26, pp. 81-86
Knirel, Y.A., Structural analysis of the lipopolysaccharide core of a rough, cystic fibrosis isolate of Pseudomonas aeruginosa (2001) Eur. J. Biochem., 268 (17), pp. 4708-4719
Kocíncová, D., Lam, J.S., Structural diversity of the core oligosaccharide domain of Pseudomonas aeruginosa lipopolysaccharide (2011) Biochemistry (Mosc.), 76 (7), pp. 755-760
Kooistra, O., Structure of a highly phosphorylated lipopolysaccharide core in the Delta algC mutants derived from Pseudomonas aeruginosa wild-type strains PAO1 (serogroup O5) and PAC1R (serogroup O3) (2003) Carbohydr. Res., 338 (23), pp. 2667-2677
Kresse, A.U., Impact of large chromosomal inversions on the adaptation and evolution of Pseudomonas aeruginosa chronically colonizing cystic fibrosis lungs (2003) Mol. Microbiol., 47, pp. 145-158
Leontein, K., Lönngren, J., Determination of the absolute configuration of sugars by gas-liquid chromatography of their acetylated 2-octyl glycosides (1978) Methods Carbohydr. Chem., 62, pp. 359-362
Masoud, H., Structural elucidation of the lipopolysaccharide core region of the O-chain-deficient mutant strain A28 from Pseudomonas aeruginosa serotype 06 (International Antigenic Typing Scheme) (1995) J. Bacteriol., 177 (23), pp. 6718-6726
Moalli, F., The therapeutic potential of the humoral pattern recognition molecule PTX3 in chronic lung infection caused by Pseudomonas aeruginosa (2011) J. Immunol., 186, pp. 5425-5434
Paroni, M., Response of CFTR-deficient mice to long-term Pseudomonas aeruginosa chronic infection and PTX3 therapy (2013) J. Infect. Dis., 208, pp. 130-138
Piantini, U., Multiple quantum filters for elucidating NMR coupling networks (1982) J. Am. Chem. Soc., 104, pp. 6800-6801
Pier, G.B., Promises and pitfalls of Pseudomonas aeruginosa lipopolysaccharide as a vaccine antigen (2003) Carbohydr. Res., 338, pp. 2549-2556
Rance, M., Improved spectral resolution in cosy 1H NMR spectra of proteins via double quantum filtering (1983) Biochem. Biophys. Res. Commun., 117, pp. 479-485
Rowe, P.S., Meadow, P.M., Structure of the core oligosaccharide from the lipopolysaccharide of Pseudomonas aeruginosa PAC1R and its defective mutants (1983) Eur. J. Biochem., 132 (2), pp. 329-337
Sadovskaya, I., Structural elucidation of the lipopolysaccharide core regions of the wild-type strain PAO1 and O-chain-deficient mutant strains AK1401 and AK1012 from Pseudomonas aeruginosa serotype O5 (1998) Eur. J. Biochem., 255 (3), pp. 673-684
Sánchez Carballo, P.M., Elucidation of the structure of an alanine-lacking core tetrasaccharide trisphosphate from the lipopolysaccharide of Pseudomonas aeruginosa mutant H4 (1999) Eur. J. Biochem., 261 (2), pp. 500-508
Silipo, A., Ammonium hydroxide hydrolysis: a valuable support in the MALDI-TOF mass spectrometry analysis of Lipid A fatty acid distribution (2002) J. Lipid Res., 43, pp. 2188-2195
Silipo, A., The structure of the phosphorylated carbohydrate backbone of the lipopolysaccharide of the phytopathogen bacterium Pseudomonas tolaasii (2004) Carbohydr. Res., 339, pp. 2241-2248
Silipo, A., Structure elucidation of the highly heterogeneous lipid A from the lipopolysaccharide of the Gram-negative extremophile bacterium Halomonas magadiensis strain 21 M1 (2004) Eur. J. Org. Chem., 10, pp. 2263-2271
Silipo, A., Full structural characterisation of the lipooligosaccharide of a Burkholderia pyrrocinia clinical isolate (2006) Eur. J. Org. Chem., 21, pp. 4874-4883
Smith, E.E., Genetic adaptation by Pseudomonas aeruginosa to the airways of cystic fibrosis patients (2006) Proc. Natl. Acad. Sci. U.S.A., 103, pp. 8487-8492
States, D.J., A two-dimensional nuclear Overhauser experiment with pure absorption phase in four quadrants (1982) J. Magn. Reson., 48, pp. 286-292
Stover, C.K., Complete genome sequence of Pseudomonas aeruginosa PA01, an opportunistic pathogen (2000) Nature, 406, pp. 959-964
Sturiale, L., Reflectron MALDI TOF and MALDI TOF/TOF mass spectrometry reveal novel structural details of native lipooligosaccharides (2011) J. Mass Spectrom., 46, pp. 1135-1142
Persistent cystic fibrosis isolate Pseudomonas aeruginosa strain RP73 exhibits an under-acylated LPS structure responsible of its low inflammatory activity
Pseudomonas aeruginosa, the major pathogen involved in lethal infections in cystic fibrosis (CF) population, is able to cause permanent chronic infections that can persist over the years. This ability to chronic colonize CF airways is related to a series of adaptive bacterial changes involving the immunostimulant lipopolysaccharide (LPS) molecule. The structure of LPSs isolated from several P. aeruginosa strains showed conserved features that can undergo chemical changes during the establishment of the chronic infection. In the present paper, we report the elucidation of the structure and the biological activity of the R-LPS (lipooligosaccharide, LOS) isolated from the persistent CF isolate P. aeruginosa strain RP73, in order to give further insights in the adaptation mechanism of the pathogen in the CF environment. The complete structural analysis of P. aeruginosa RP73 LOS was achieved by chemical analyses, NMR spectroscopy and MALDI MS spectrometry, while the assessment of the biological activity was attained testing the in vivo pro-inflammatory capacity of the isolated LOS molecule. While a typical CF LPS is able to trigger a high immune response and production of pro-inflammatory molecules, this P. aeruginosa RP73 LOS showed to possess a low pro-inflammatory capacity. This was possible due to a singular chemical structure possessing an under-acylated lipid A very similar to the LPS of P. aeruginosa found in chronic lung diseases such as bronchiectstasis. © 2014 Elsevier Ltd.
Pseudomonas aeruginosa, the major pathogen involved in lethal infections in cystic fibrosis (CF) population, is able to cause permanent chronic infections that can persist over the years. This ability to chronic colonize CF airways is related to a series of adaptive bacterial changes involving the immunostimulant lipopolysaccharide (LPS) molecule. The structure of LPSs isolated from several P. aeruginosa strains showed conserved features that can undergo chemical changes during the establishment of the chronic infection. In the present paper, we report the elucidation of the structure and the biological activity of the R-LPS (lipooligosaccharide, LOS) isolated from the persistent CF isolate P. aeruginosa strain RP73, in order to give further insights in the adaptation mechanism of the pathogen in the CF environment. The complete structural analysis of P. aeruginosa RP73 LOS was achieved by chemical analyses, NMR spectroscopy and MALDI MS spectrometry, while the assessment of the biological activity was attained testing the in vivo pro-inflammatory capacity of the isolated LOS molecule. While a typical CF LPS is able to trigger a high immune response and production of pro-inflammatory molecules, this P. aeruginosa RP73 LOS showed to possess a low pro-inflammatory capacity. This was possible due to a singular chemical structure possessing an under-acylated lipid A very similar to the LPS of P. aeruginosa found in chronic lung diseases such as bronchiectstasis. © 2014 Elsevier Ltd.
Persistent cystic fibrosis isolate Pseudomonas aeruginosa strain RP73 exhibits an under-acylated LPS structure responsible of its low inflammatory activity
| ▼ Peptides of therapeutic interest with antimicrobial and/ or antitumoral activity |
Persistent cystic fibrosis isolate Pseudomonas aeruginosa strain RP73 exhibits an under-acylated LPS structure responsible of its low inflammatory activity
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Musumeci D, Roviello GN, Montesarchio D
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Impact Factor: 9.723
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Impact Factor: 3.534
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Avitabile C, Netti F, Orefice G, Palmieri M, Nocerino N, Malgieri G, D'Andrea LD, Capparelli R, Fattorusso R, Romanelli A
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Scudiero O, Galdiero S, Nigro E, Del Vecchio L, Di Noto R, Cantisani M, Colavita I, Galdiero M, Cassiman JJ, Daniele A, Pedone C, Salvatore F
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Scalia M, Satriano C, Greca R, Stella AM, Rizzarelli E, Spina-Purrello V
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Sorriento D, Fusco A, Ciccarelli M, Rungi A, Anastasio A, Carillo A, Dorn II GW, Trimarco B, Iaccarino G
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Impact Factor: 3.341
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Dolga AM, Letsche T, Gold M, Doti N, Bacher M, Chiamvimonvat N, Dodel R, Culmsee C
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Berisio R
* Learning from pathogens: Exploiting host-pathogen liaison for therapeutic development (554 views)
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Impact Factor: 2.326
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Galdiero S, Falanga A, Cantisani M, Tarallo R, della Pepa ME, D'Oriano V, Galdiero M
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Impact Factor: 2.326
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Berisio R
* Editorial: cell processes in bacterial diseases (409 views)
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Impact Factor: 1.994
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De Simone G
* Editorial: Zinc Metallo-Enzymes As Target For Drug Design (443 views)
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Impact Factor: 4.07
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Musumeci D, Bucci EM, Roviello GN, Sapio R, Valente M, Moccia M, Bianchi ME, Pedone C
* DNA-based strategies for blocking HMGB1 cytokine activity: Design, synthesis and preliminary in vitro/in vivo assays of DNA and DNA-like duplexes (481 views)
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Impact Factor: 3.534
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Spina-Purrello V, Giliberto S, Barresi V, Nicoletti VG, Giuffrida Stella AM, Rizzarelli E
* Modulation of PARP-1 and PARP-2 expression by L-carnosine and trehalose after LPS and INFγ-induced oxidative stress (450 views)
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Impact Factor: 2.608
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Gautier B, Goncalves V, Diana D, Di Stasi R, Teillet F, Lenoir C, Huguenot F, Garbay C, Fattorusso R, D'Andrea LD, Vidal M, Inguimbert N
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Impact Factor: 5.207
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Diana D, Ziaco B, Scarabelli G, Pedone C, Colombo G, D'Andrea LD, Fattorusso R
* Structural analysis of a helical peptide unfolding pathway (507 views)
Chemistry (ISSN: 1521-3765, 0947-6539, 1521-3765electronic), 2010 May 10; 16(18): 5400-5407.
Impact Factor: 5.476
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D'Ambrosio K, Limauro D, Pedone E, Galdi I, Pedone C, Bartolucci S, De Simone G
* Insights into the catalytic mechanism of the Bcp family: functional and structural analysis of Bcp1 from Sulfolobus solfataricus (554 views)
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Impact Factor: 3.085
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Di Fiore A, Fiorentino G, Vitale RM, Ronca R, Amodeo P, Pedone C, Bartolucci S, De Simone G
* Structural analysis of BldR from Sulfolobus solfataricus provides insights into the molecular basis of transcriptional activation in Archaea by MarR family proteins (470 views)
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Impact Factor: 3.871
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Daniele A, Cardillo G, Pennino C, Carbone MT, Scognamiglio D, Esposito L, Correra A, Castaldo G, Zagari A, Salvatore F
* Five human phenylalanine hydroxylase proteins identified in mild hyperphenylalaninemia patients are disease-causing variants (361 views)
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Impact Factor: 2.233
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Sorriento D, Ciccarelli M, Santulli G, Campanile A, Altobelli GG, Cimini V, Galasso G, Astone D, Piscione F, Pastore L, Trimarco B, Iaccarino G
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Impact Factor: 9.38
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Nicoletti VG, Santoro AM, Grasso G, Vagliasindi LI, Giuffrida ML, Cuppari C, Purrello VS, Stella AMG, Rizzarelli E
* Carnosine interaction with nitric oxide and astroglial cell protection (668 views)
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Impact Factor: 3.268
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Ruggiero A, Chambery A, Di Maro A, Mastroianni A, Parente A, Berisio R
* Crystallization and preliminary X-ray diffraction analysis of PD-L1, a highly glycosylated ribosome inactivating protein with DNase activity (487 views)
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Santelli E, Leone M, Li C, Fukushima T, Preece NE, Olson AJ, Ely KR, Reed JC, Pellecchia M, Liddington RC, Matsuzawa S-I
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Calabrese V, Colombrita C, Guagliano E, Sapienza M, Ravagna A, Cardile V, Scapagnini G, Santoro AM, Mangiameli A, Butterfield DA, Giuffrida Stella AM, Rizzarelli E
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Paesano N, Marzocco S, Vicidomini C, Saturnino C, Autore G, De Martino G, Sbardella G
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De Simone G, Mandrich L, Menchise V, Giordano V, Febbraio F, Rossi M, Pedone C, Manco G
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Galdiero M, Vitiello M, Galdiero S
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Borgatti M, Romanelli A, Saviano M, Pedone C, Lampronti I, Breda L, Nastruzzi C, Bianchi N, Mischiati C, Gambari R
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Ogawa R, Pacelli R, Espey MG, Miranda KM, Friedman N, Kim SM, Cox G, Mitchell JB, Wink DA, Russo A
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Saviano M, Isernia C, Rossi F, Di Blasio B, Iacovino R, Mazzeo M, Pedone C, Benedetti E
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Pavone V, De Simone G, Nastri F, Galdiero S, Staiano N, Lombardi A, Pedone C
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Lombardi A, Maglio O, Benedetti B, Di Blasio E, Saviano M, Nastri F, Pedone C, Pavone V
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Sandomenico A, Ruggiero A, Iaccarino E, Oliver A, Squeglia F, Moreira M, Esposito L, Ruvo M, Berisio R
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Monti A, Sturlese M, Caporale A, Roger JA, Mascanzoni F, Ruvo M, Doti N
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Mercurio FA, Pirone L, Di Natale C, Marasco D, Pedone EM, Leone M
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Monti M, De Rosa V, Iommelli F, Carriero MV, Terlizzi C, Camerlingo R, Belli S, Fonti R, Di Minno G, Del Vecchio S
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Dennehy R, RomanoM, Ruggiero A, Mohamed YF, Dignam SL, Mujica Troncoso C, Callaghan M, Valvano MA, Berisio R, Mcclean S
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Monti M, Iommelli F, De Rosa V, Carriero MV, Miceli R, Camerlingo R, Di Minno G, Del Vecchio S
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Speciale I, Paciello I, Fazio LL, Sturiale L, Palmigiano A, Lanzetta R, Parrilli M, Garozzo D, Lemaitre B, Bernardini ML, Molinaro A, De Castro C
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Avitabile C, D'Andrea LD, Romanelli A
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Impact Factor: 5.578
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Musumeci D, Roviello GN, Montesarchio D
* An overview on HMGB1 inhibitors as potential therapeutic agents in HMGB1-related pathologies (559 views)
Pharmacol Ther (ISSN: 0163-7258, 0163-7258linking), 2014 Mar; 141(3): 347-357.
Impact Factor: 9.723
View Export to BibTeX Export to EndNote
Montefusco S, Esposito R, D'Andrea L, Monti MC, Dunne C, Dolan B, Tosco A, Marzullo L, Clyne M
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Impact Factor: 3.534
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Avitabile C, Netti F, Orefice G, Palmieri M, Nocerino N, Malgieri G, D'Andrea LD, Capparelli R, Fattorusso R, Romanelli A
* Design, structural and functional characterization of a Temporin-1b analog active against Gram-negative bacteria (490 views)
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Impact Factor: 3.829
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Scudiero O, Galdiero S, Nigro E, Del Vecchio L, Di Noto R, Cantisani M, Colavita I, Galdiero M, Cassiman JJ, Daniele A, Pedone C, Salvatore F
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Impact Factor: 4.451
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Scalia M, Satriano C, Greca R, Stella AM, Rizzarelli E, Spina-Purrello V
* PARP-1 inhibitors DPQ and PJ-34 negatively modulate proinflammatory commitment of human glioblastoma cells (439 views)
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Impact Factor: 2.551
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Sorriento D, Fusco A, Ciccarelli M, Rungi A, Anastasio A, Carillo A, Dorn II GW, Trimarco B, Iaccarino G
* Mitochondrial G protein coupled receptor kinase 2 regulates proinflammatory responses in macrophages (428 views)
Febs Lett (ISSN: 0014-5793, 0014-5793print, 1873-3468electronic), 2013; 587(21): 3487-3494.
Impact Factor: 3.341
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Dolga AM, Letsche T, Gold M, Doti N, Bacher M, Chiamvimonvat N, Dodel R, Culmsee C
* Activation of KCNN3/SK3/K(Ca)2. 3 channels attenuates enhanced calcium influx and inflammatory cytokine production in activated microglia (557 views)
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Impact Factor: 5.466
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Berisio R
* Learning from pathogens: Exploiting host-pathogen liaison for therapeutic development (554 views)
Curr Protein Pept Sci (ISSN: 1389-2037, 1389-2037linking), 2012 Dec; 13(8): 697-698.
Impact Factor: 2.326
View Export to BibTeX Export to EndNote
Galdiero S, Falanga A, Cantisani M, Tarallo R, della Pepa ME, D'Oriano V, Galdiero M
* Microbe-Host Interactions: Structure and Role of Gram-Negative Bacterial Porins (677 views)
Curr Protein Pept Sci (ISSN: 1389-2037, 1389-2037linking), 2012 Dec; 13(8): 843-854.
Impact Factor: 2.326
View Export to BibTeX Export to EndNote
Berisio R
* Editorial: cell processes in bacterial diseases (409 views)
Protein Pept Lett (ISSN: 0929-8665, 1875-5305), 2012 Oct; 19(10): 1011-1012.
Impact Factor: 1.994
View Export to BibTeX Export to EndNote
De Simone G
* Editorial: Zinc Metallo-Enzymes As Target For Drug Design (443 views)
Curr Med Chem (ISSN: 0929-8673, 1875-533x, 1875-533xelectronic), 2012 Feb; 19(6): 820-1012.
Impact Factor: 4.07
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Musumeci D, Bucci EM, Roviello GN, Sapio R, Valente M, Moccia M, Bianchi ME, Pedone C
* DNA-based strategies for blocking HMGB1 cytokine activity: Design, synthesis and preliminary in vitro/in vivo assays of DNA and DNA-like duplexes (481 views)
Mol Biosyst (ISSN: 1742-206x, 1742-2051, 1742-2051electronic), 2011 May; 7(5): 1742-1752.
Impact Factor: 3.534
View Export to BibTeX Export to EndNote
Spina-Purrello V, Giliberto S, Barresi V, Nicoletti VG, Giuffrida Stella AM, Rizzarelli E
* Modulation of PARP-1 and PARP-2 expression by L-carnosine and trehalose after LPS and INFγ-induced oxidative stress (450 views)
Neurochemical Research (ISSN: 0364-3190, 1573-6903), 2010 Dec; 35(12): 2144-2153.
Impact Factor: 2.608
View Export to BibTeX Export to EndNote
Gautier B, Goncalves V, Diana D, Di Stasi R, Teillet F, Lenoir C, Huguenot F, Garbay C, Fattorusso R, D'Andrea LD, Vidal M, Inguimbert N
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Impact Factor: 5.207
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Diana D, Ziaco B, Scarabelli G, Pedone C, Colombo G, D'Andrea LD, Fattorusso R
* Structural analysis of a helical peptide unfolding pathway (507 views)
Chemistry (ISSN: 1521-3765, 0947-6539, 1521-3765electronic), 2010 May 10; 16(18): 5400-5407.
Impact Factor: 5.476
View Export to BibTeX Export to EndNote
D'Ambrosio K, Limauro D, Pedone E, Galdi I, Pedone C, Bartolucci S, De Simone G
* Insights into the catalytic mechanism of the Bcp family: functional and structural analysis of Bcp1 from Sulfolobus solfataricus (554 views)
Proteins (ISSN: 1097-0134, 0887-3585, 1097-0134electronic), 2009 Sep; 76(4): 995-1006.
Impact Factor: 3.085
View Export to BibTeX Export to EndNote
Di Fiore A, Fiorentino G, Vitale RM, Ronca R, Amodeo P, Pedone C, Bartolucci S, De Simone G
* Structural analysis of BldR from Sulfolobus solfataricus provides insights into the molecular basis of transcriptional activation in Archaea by MarR family proteins (470 views)
J Mol Biol (ISSN: 1089-8638, 0022-2836, 1089-8638electronic), 2009 May 8; 388(3): 559-569.
Impact Factor: 3.871
View Export to BibTeX Export to EndNote
Daniele A, Cardillo G, Pennino C, Carbone MT, Scognamiglio D, Esposito L, Correra A, Castaldo G, Zagari A, Salvatore F
* Five human phenylalanine hydroxylase proteins identified in mild hyperphenylalaninemia patients are disease-causing variants (361 views)
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Impact Factor: 2.233
View Export to BibTeX Export to EndNote
Sorriento D, Ciccarelli M, Santulli G, Campanile A, Altobelli GG, Cimini V, Galasso G, Astone D, Piscione F, Pastore L, Trimarco B, Iaccarino G
* The G-protein-coupled receptor kinase 5 inhibits NFκB transcriptional activity by inducing nuclear accumulation of IκBα (438 views)
P Natl Acad Sci Usa (ISSN: 0027-8424, 1091-6490, 0027-8424print), 2008; 105(46): 17818-17823.
Impact Factor: 9.38
View Export to BibTeX Export to EndNote
Nicoletti VG, Santoro AM, Grasso G, Vagliasindi LI, Giuffrida ML, Cuppari C, Purrello VS, Stella AMG, Rizzarelli E
* Carnosine interaction with nitric oxide and astroglial cell protection (668 views)
Journal Of Neuroscience Research (ISSN: 0360-4012), 2007 Sep 1; 85(10): 2239-2245.
Impact Factor: 3.268
View Export to BibTeX Export to EndNote
Ruggiero A, Chambery A, Di Maro A, Mastroianni A, Parente A, Berisio R
* Crystallization and preliminary X-ray diffraction analysis of PD-L1, a highly glycosylated ribosome inactivating protein with DNase activity (487 views)
Protein Pept Lett (ISSN: 0929-8665, 1875-5305), 2007; 14(4): 407-409.
Impact Factor: 1.097
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Santelli E, Leone M, Li C, Fukushima T, Preece NE, Olson AJ, Ely KR, Reed JC, Pellecchia M, Liddington RC, Matsuzawa S-I
* Structural analysis of Siah1-Siah-interacting protein interactions and insights into the assembly of an E3 ligase multiprotein complex (408 views)
Jbc Papers (ISSN: 0021-9258, 1083-351x, 0021-9258linking), 2005 Oct 7; 280(40): 34278-34287.
Impact Factor: 5.854
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Calabrese V, Colombrita C, Guagliano E, Sapienza M, Ravagna A, Cardile V, Scapagnini G, Santoro AM, Mangiameli A, Butterfield DA, Giuffrida Stella AM, Rizzarelli E
* Protective effect of carnosine during nitrosative stress in astroglial cell cultures (711 views)
Neurochemical Research (ISSN: 0364-3190, 1573-6903), 2005 Jun; 30(6-7): 797-807.
Impact Factor: 2.187
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Paesano N, Marzocco S, Vicidomini C, Saturnino C, Autore G, De Martino G, Sbardella G
* Synthesis and biological evaluation of 3-benzyl-1-methyl- and 1-methyl-3-phenyl-isothioureas as potential inhibitors of iNOS (465 views)
Bioorganic & Medicinal Chemistry Letters (ISSN: 0960-894x), 2005 Feb 1; 15(3): 539-543.
Impact Factor: 2.478
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De Simone G, Mandrich L, Menchise V, Giordano V, Febbraio F, Rossi M, Pedone C, Manco G
* A substrate-induced switch in the reaction mechanism of a thermophilic esterase - Kinetic evidences and structural basis (409 views)
Jbc Papers (ISSN: 0021-9258, 1083-351x, 0021-9258linking), 2004 Feb 20; 279(8): 6815-6823.
Impact Factor: 6.355
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Galdiero M, Vitiello M, Galdiero S
* Eukaryotic cell signaling and transcriptional activation induced by bacterial porins (549 views)
Fems Microbiology Letters (ISSN: 0378-1097), 2003 Sep 12; 226(1): 57-64.
Impact Factor: 1.932
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Borgatti M, Romanelli A, Saviano M, Pedone C, Lampronti I, Breda L, Nastruzzi C, Bianchi N, Mischiati C, Gambari R
* Resistance of decoy PNA-DNA chimeras to enzymatic degradation in cellular extracts and serum (430 views)
Oncology Research (ISSN: 0965-0407), 2003; 13(5): 279-287.
Impact Factor: 1.794
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Ogawa R, Pacelli R, Espey MG, Miranda KM, Friedman N, Kim SM, Cox G, Mitchell JB, Wink DA, Russo A
* Comparison of control of Listeria by nitric oxide redox chemistry from murine macrophages and no donors: Insights into listeriocidal activity of oxidative and nitrosative stress (776 views)
Free Radic Biol Med (ISSN: 0891-5849, 0891-5849linking, 1873-4596electronic), 2001 Feb 1; 30(3): 268-276.
Impact Factor: 5.082
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Saviano M, Isernia C, Rossi F, Di Blasio B, Iacovino R, Mazzeo M, Pedone C, Benedetti E
Solid state structural analysis of the cyclooctapeptide cyclo-(Pro(1)-Pro-Phe-Phe-Ac(6)c-Ile-D-Ala-Val(8)) (429 views)
Biopolymers (ISSN: 0006-3525, 0006-6352, 0006-3525print), 2000 Feb; 53(2): 189-199.
Impact Factor: 2.405
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Pavone V, De Simone G, Nastri F, Galdiero S, Staiano N, Lombardi A, Pedone C
Multiple binding mode of reversible synthetic thrombin inhibitors. A comparative structural analysis (591 views)
Biol Chem Biological Chemistry (ISSN: 1431-6730, 1437-4315), 1998 Sep; 379(8-9): 987-1006.
Impact Factor: 2.636
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Lombardi A, Maglio O, Benedetti B, Di Blasio E, Saviano M, Nastri F, Pedone C, Pavone V
Pt(II) Complexes of Amino Acids and Peptides. II. Structural Analysis of trans-[Cl2-Pt-(H-Aib-OH)2] (429 views)
Inorg Chim Acta, 1992; 196: 241-246.
Impact Factor: 0
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Sandomenico A, Ruggiero A, Iaccarino E, Oliver A, Squeglia F, Moreira M, Esposito L, Ruvo M, Berisio R
Unveiling CD59-Antibody Interactions to Design Paratope-Mimicking Peptides for Complement Modulation (2 views)
Int J Mol Sci (ISSN: 1422-0067linking), N/D; 21222223241031126119: 500-519.
Impact Factor: 6.21
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44 Records (43 excluding Abstracts).
Total impact factor: 168.861 (167.085 excluding Abstracts).
Total 5 year impact factor: 166.641 (164.678 excluding Abstracts).
Total impact factor: 168.861 (167.085 excluding Abstracts).
Total 5 year impact factor: 166.641 (164.678 excluding Abstracts).
367 views. Last view on Tuesday 30 May 2023, 16:40:22
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