Renal pH Mapping Using Chemical Exchange Saturation Transfer (CEST) MRI: Experimental Protocol(247 views) Pavuluri KD, Consolino L, Longo DL, Irrera P, Sun PZ, Mcmahon MT
Paper type: Journal Article, Research Support, N. I. H. , Extramural, Non-U. S. Gov'T,
Impact factor: 1.17, 5-year impact factor: 4.197
Url: Not available.
Keywords: Animals, Contrast Media Chemistry, Hydrogen-Ion Concentration, Image Processing, Computer-Assisted Methods, Kidney Physiology, Magnetic Resonance Imaging Methods, Inbred C57bl, Monitoring, Physiologic Methods, Phantoms, Software, Chemical Exchange Saturation Transfer (cest), Contrast Agent, Iopamidol, Magnetic Resonance Imaging (mri), Responsive Contrast Agent, Ph Imaging,
Affiliations: *** IBB - CNR ***
Division of MR Research, The Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Department of Molecular Biotechnology and Health Sciences, University of Torino, Torino, Italy.
Institute of Biostructures and Bioimaging (IBB), Italian National Research Council (CNR), Torino, Italy.
University of Campania "Luigi Vanvitelli", Naples, Italy.
Yerkes Imaging Center, Yerkes National Primate Research Center, Emory University, Atlanta, GA, USA.
Department of Radiology and Imaging Sciences, Emory University School of Medicine, Atlanta, GA, USA.
Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA, USA.
F.M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Institute, Baltimore, MD, USA. mtmcmaho@gmail.com.
References: Not available.
Renal pH Mapping Using Chemical Exchange Saturation Transfer (CEST) MRI: Experimental Protocol
Chemical exchange saturation transfer (CEST) is recognized as one of the premier methods for measuring pH with this environmental variable expected to be an excellent biomarker for kidney diseases. Here we describe step-by-step CEST MRI experimental protocols for producing pH and perfusion maps for monitoring kidney pH homeostasis in rodents after administering iopamidol as contrast agent. Several CEST techniques, acquisition protocols and ratiometric approaches are described. The impact of length of acquisition time on the quality of the maps is detailed. These methods may be useful for investigating progression in kidney disease in vivo for rodent models.This chapter is based upon work from the COST Action PARENCHIMA, a community-driven network funded by the European Cooperation in Science and Technology (COST) program of the European Union, which aims to improve the reproducibility and standardization of renal MRI biomarkers. This experimental protocol is complemented by two separate chapters describing the basic concepts and data analysis.
Renal pH Mapping Using Chemical Exchange Saturation Transfer (CEST) MRI: Experimental Protocol
Hesse B, Tagil K, Cuocolo A, Anagnostopoulos C, Bardies M, Bax J, Bengel F, Busemann Sokole E, Davies G, Dondi M, Edenbrandt L, Franken P, Kjaer A, Knuuti J, Lassmann M, Ljungberg M, Marcassa C, Marie PY, Mckiddie F, O'connor M, Prvuolovich E, Underwood R * 3. 0 T perfusion MR imaging(1028 views) Rivista Di Neuroradiologia (ISSN: 1120-9976), 2004; 17(6): 807-812. Impact Factor:0.023 ViewExport to BibTeXExport to EndNote