Mutational and structural study of RipA, a key enzyme in Mycobacterium tuberculosis cell division: Evidence for the l-to-d inversion of configuration of the catalytic cysteine (487 views)
Acta Crystallogr D Biol Crystallogr (ISSN: 0907-4449, 0907-4449linking), 2014 Sep; 70(9): 2295-2300.
Export to BibTeX Export to EndNote
Paper type: Journal Article, Research Support, Non-U. S. Gov'T,
Impact factor: 14.1, 5-year impact factor: 7.5
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84907016009&partnerID=40&md5=2e2026bf75033d2ff67f655f6245418b
Keywords: Cysteine Protease, Mycobacterium Tuberculosis,
Affiliations:
*** IBB - CNR ***
Institute of Biostructures and Bioimaging, CNRNaples, Italy
Dipartimento di Scienze Ambientali, Seconda Università di NapoliCaserta, Italy
References:
Amano, M., Hasegawa, J., Kobayashi, N., Kishi, N., Nakazawa, T., Uchiyama, S., Fukui, K., (2011) Anal. Chem., 83, pp. 3857-386
Böth, D., Schneider, G., Schnell, R., (2011) J. Mol. Biol., 413, pp. 247-260
Buczek, O., Yoshikami, D., Bulaj, G., Jimenez, E.C., Olivera, B.M., (2005) J. Biol. Chem., 280, pp. 4247-4253
Chao, M.C., Kieser, K.J., Minami, S., Mavrici, D., Aldridge, B.B., Fortune, S.M., Alber, T., Rubin, E.J., (2013) PLoS Pathog., 9, p. e1003197
Cloos, P.A., Christgau, S., (2002) Matrix Biol., 21, pp. 39-52
Correale, S., Ruggiero, A., Capparelli, R., Pedone, E., Berisio, R., (2013) Acta Cryst., D69, pp. 1697-1706
Goldenberg, O., Erez, E., Nimrod, G., Ben-Tal, N., (2009) Nucleic Acids Res., 37, pp. D323-D327
Heck, S.D., (1994) Science, 266, pp. 1065-1068
Hett, E.C., Chao, M.C., Deng, L.L., Rubin, E.J., (2008) PLoS Pathog., 4, p. e1000001
Hett, E.C., Rubin, E.J., (2008) Microbiol. Mol. Biol. Rev., 72, pp. 126-156
Kamphuis, I.G., Kalk, K.H., Swarte, M.B., Drenth, J., (1984) J. Mol. Biol., 179, pp. 233-256
Kreil, G., (1994) Science, 266, pp. 996-997
Laskowski, R.A., Rullmannn, J.A., MacArthur, M.W., Kaptein, R., Thornton, J.M., (1996) J. Biomol. NMR, 8, pp. 477-486
Layec, S., Decaris, B., Leblond-Bourget, N., (2008) Res. Microbiol., 159, pp. 507-515
Layec, S., Gérard, J., Legué, V., Chapot-Chartier, M.-P., Courtin, P., Borges, F., Decaris, B., Leblond-Bourget, N., (2009) Mol Microbiol., 71, pp. 1205-1217
Lindorff-Larsen, K., Piana, S., Palmo, K., Maragakis, P., Klepeis, J.L., Dror, R.O., Shaw, D.E., (2010) Proteins, 78, pp. 1950-1958
Martínez-Caballero, S., Lee, M., Artola-Recolons, C., Carrasco-López, C., Hesek, D., Spink, E., Lastochkin, E., Hermoso, J.A., (2013) J. Am. Chem. Soc., 135, pp. 10318-10321
Meroueh, S.O., Bencze, K.Z., Hesek, D., Lee, M., Fisher, J.F., Stemmler, T.L., Mobashery, S., (2006) Proc. Natl Acad. Sci. USA, 103, pp. 4404-4409
Murshudov, G.N., Skubák, P., Lebedev, A.A., Pannu, N.S., Steiner, R.A., Nicholls, R.A., Winn, M.D., Vagin, A.A., (2011) Acta Cryst., D67, pp. 355-367
Nikitushkin, V.D., Demina, G.R., Shleeva, M.O., Kaprelyants, A.S., (2013) Antonie Van Leeuwenhoek, 103, pp. 37-46
Otwinowski, Z., Minor, W., (1997) Methods Enzymol., 276, pp. 307-326
Rossi, P., Aramini, J.M., Xiao, R., Chen, C.X., Nwosu, C., Owens, L.A., Maglaqui, M., Montelione, G.T., (2009) Proteins, 74, pp. 515-519
Ruggiero, A., De Simone, P., Smaldone, G., Squeglia, F., Berisio, R., (2012) Curr. Protein Pept. Sci., 13, pp. 756-766
Ruggiero, A., Marasco, D., Squeglia, F., Soldini, S., Pedone, E., Pedone, C., Berisio, R., (2010) Structure, 18, pp. 1184-1190
Ruggiero, A., Marchant, J., Squeglia, F., Makarov, V., De Simone, A., Berisio, R., (2013) J. Biomol. Struct. Dyn., 31, pp. 195-205
Ruggiero, A., Squeglia, F., Esposito, C., Marasco, D., Pedone, E., Pedone, C., Berisio, R., (2010) Protein Pept. Lett., 17, pp. 70-73
Sheldrick, G.M., (2008) Acta Cryst., A64, pp. 112-122
Squeglia, F., Marchetti, R., Ruggiero, A., Lanzetta, R., Marasco, D., Dworkin, J., Petoukhov, M., Silipo, A., (2011) J. Am. Chem. Soc., 133, pp. 20676-20679
Squeglia, F., Romano, M., Ruggiero, A., Vitagliano, L., De Simone, A., Berisio, R., (2013) Biophys. J., 104, pp. 2530-2539
Storer, A.C., Ménard, R., (1994) Methods Enzymol., 244, pp. 486-500
Tomiyama, T., Asano, S., Furiya, Y., Shirasawa, T., Endo, N., Mori, H., (1994) J. Biol. Chem., 269, pp. 10205-10208
Winn, M.D., (2011) Acta Cryst., D67, pp. 235-242
Xu, Q., (2010) Acta Cryst., F66, pp. 1354-1364
Zhang, W., Sulea, T., Tao, L., Cui, Q., Purisima, E.O., Vongsamphanh, R., Lachance, P., Ménard, R., (2011) Biochemistry, 50, pp. 4775-4775
B th, D., Schneider, G., Schnell, R., (2011) J. Mol. Biol., 413, pp. 247-260
Chao, M. C., Kieser, K. J., Minami, S., Mavrici, D., Aldridge, B. B., Fortune, S. M., Alber, T., Rubin, E. J., (2013) PLoS Pathog., 9, p. e1003197
Cloos, P. A., Christgau, S., (2002) Matrix Biol., 21, pp. 39-52
Heck, S. D., (1994) Science, 266, pp. 1065-1068
Hett, E. C., Chao, M. C., Deng, L. L., Rubin, E. J., (2008) PLoS Pathog., 4, p. e1000001
Hett, E. C., Rubin, E. J., (2008) Microbiol. Mol. Biol. Rev., 72, pp. 126-156
Kamphuis, I. G., Kalk, K. H., Swarte, M. B., Drenth, J., (1984) J. Mol. Biol., 179, pp. 233-256
Laskowski, R. A., Rullmannn, J. A., MacArthur, M. W., Kaptein, R., Thornton, J. M., (1996) J. Biomol. NMR, 8, pp. 477-486
Layec, S., G rard, J., Legu, V., Chapot-Chartier, M. -P., Courtin, P., Borges, F., Decaris, B., Leblond-Bourget, N., (2009) Mol Microbiol., 71, pp. 1205-1217
Mart nez-Caballero, S., Lee, M., Artola-Recolons, C., Carrasco-L pez, C., Hesek, D., Spink, E., Lastochkin, E., Hermoso, J. A., (2013) J. Am. Chem. Soc., 135, pp. 10318-10321
Meroueh, S. O., Bencze, K. Z., Hesek, D., Lee, M., Fisher, J. F., Stemmler, T. L., Mobashery, S., (2006) Proc. Natl Acad. Sci. USA, 103, pp. 4404-4409
Murshudov, G. N., Skub k, P., Lebedev, A. A., Pannu, N. S., Steiner, R. A., Nicholls, R. A., Winn, M. D., Vagin, A. A., (2011) Acta Cryst., D67, pp. 355-367
Nikitushkin, V. D., Demina, G. R., Shleeva, M. O., Kaprelyants, A. S., (2013) Antonie Van Leeuwenhoek, 103, pp. 37-46
Sheldrick, G. M., (2008) Acta Cryst., A64, pp. 112-122
Storer, A. C., M nard, R., (1994) Methods Enzymol., 244, pp. 486-500
Winn, M. D., (2011) Acta Cryst., D67, pp. 235-242
Acta Crystallogr D Biol Crystallogr (ISSN: 0907-4449, 0907-4449linking), 2014 Sep; 70(9): 2295-2300.
Export to BibTeX Export to EndNote
Paper type: Journal Article, Research Support, Non-U. S. Gov'T,
Impact factor: 14.1, 5-year impact factor: 7.5
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84907016009&partnerID=40&md5=2e2026bf75033d2ff67f655f6245418b
Keywords: Cysteine Protease, Mycobacterium Tuberculosis,
Affiliations:
*** IBB - CNR ***
Institute of Biostructures and Bioimaging, CNRNaples, Italy
Dipartimento di Scienze Ambientali, Seconda Università di NapoliCaserta, Italy
References:
Amano, M., Hasegawa, J., Kobayashi, N., Kishi, N., Nakazawa, T., Uchiyama, S., Fukui, K., (2011) Anal. Chem., 83, pp. 3857-386
Böth, D., Schneider, G., Schnell, R., (2011) J. Mol. Biol., 413, pp. 247-260
Buczek, O., Yoshikami, D., Bulaj, G., Jimenez, E.C., Olivera, B.M., (2005) J. Biol. Chem., 280, pp. 4247-4253
Chao, M.C., Kieser, K.J., Minami, S., Mavrici, D., Aldridge, B.B., Fortune, S.M., Alber, T., Rubin, E.J., (2013) PLoS Pathog., 9, p. e1003197
Cloos, P.A., Christgau, S., (2002) Matrix Biol., 21, pp. 39-52
Correale, S., Ruggiero, A., Capparelli, R., Pedone, E., Berisio, R., (2013) Acta Cryst., D69, pp. 1697-1706
Goldenberg, O., Erez, E., Nimrod, G., Ben-Tal, N., (2009) Nucleic Acids Res., 37, pp. D323-D327
Heck, S.D., (1994) Science, 266, pp. 1065-1068
Hett, E.C., Chao, M.C., Deng, L.L., Rubin, E.J., (2008) PLoS Pathog., 4, p. e1000001
Hett, E.C., Rubin, E.J., (2008) Microbiol. Mol. Biol. Rev., 72, pp. 126-156
Kamphuis, I.G., Kalk, K.H., Swarte, M.B., Drenth, J., (1984) J. Mol. Biol., 179, pp. 233-256
Kreil, G., (1994) Science, 266, pp. 996-997
Laskowski, R.A., Rullmannn, J.A., MacArthur, M.W., Kaptein, R., Thornton, J.M., (1996) J. Biomol. NMR, 8, pp. 477-486
Layec, S., Decaris, B., Leblond-Bourget, N., (2008) Res. Microbiol., 159, pp. 507-515
Layec, S., Gérard, J., Legué, V., Chapot-Chartier, M.-P., Courtin, P., Borges, F., Decaris, B., Leblond-Bourget, N., (2009) Mol Microbiol., 71, pp. 1205-1217
Lindorff-Larsen, K., Piana, S., Palmo, K., Maragakis, P., Klepeis, J.L., Dror, R.O., Shaw, D.E., (2010) Proteins, 78, pp. 1950-1958
Martínez-Caballero, S., Lee, M., Artola-Recolons, C., Carrasco-López, C., Hesek, D., Spink, E., Lastochkin, E., Hermoso, J.A., (2013) J. Am. Chem. Soc., 135, pp. 10318-10321
Meroueh, S.O., Bencze, K.Z., Hesek, D., Lee, M., Fisher, J.F., Stemmler, T.L., Mobashery, S., (2006) Proc. Natl Acad. Sci. USA, 103, pp. 4404-4409
Murshudov, G.N., Skubák, P., Lebedev, A.A., Pannu, N.S., Steiner, R.A., Nicholls, R.A., Winn, M.D., Vagin, A.A., (2011) Acta Cryst., D67, pp. 355-367
Nikitushkin, V.D., Demina, G.R., Shleeva, M.O., Kaprelyants, A.S., (2013) Antonie Van Leeuwenhoek, 103, pp. 37-46
Otwinowski, Z., Minor, W., (1997) Methods Enzymol., 276, pp. 307-326
Rossi, P., Aramini, J.M., Xiao, R., Chen, C.X., Nwosu, C., Owens, L.A., Maglaqui, M., Montelione, G.T., (2009) Proteins, 74, pp. 515-519
Ruggiero, A., De Simone, P., Smaldone, G., Squeglia, F., Berisio, R., (2012) Curr. Protein Pept. Sci., 13, pp. 756-766
Ruggiero, A., Marasco, D., Squeglia, F., Soldini, S., Pedone, E., Pedone, C., Berisio, R., (2010) Structure, 18, pp. 1184-1190
Ruggiero, A., Marchant, J., Squeglia, F., Makarov, V., De Simone, A., Berisio, R., (2013) J. Biomol. Struct. Dyn., 31, pp. 195-205
Ruggiero, A., Squeglia, F., Esposito, C., Marasco, D., Pedone, E., Pedone, C., Berisio, R., (2010) Protein Pept. Lett., 17, pp. 70-73
Sheldrick, G.M., (2008) Acta Cryst., A64, pp. 112-122
Squeglia, F., Marchetti, R., Ruggiero, A., Lanzetta, R., Marasco, D., Dworkin, J., Petoukhov, M., Silipo, A., (2011) J. Am. Chem. Soc., 133, pp. 20676-20679
Squeglia, F., Romano, M., Ruggiero, A., Vitagliano, L., De Simone, A., Berisio, R., (2013) Biophys. J., 104, pp. 2530-2539
Storer, A.C., Ménard, R., (1994) Methods Enzymol., 244, pp. 486-500
Tomiyama, T., Asano, S., Furiya, Y., Shirasawa, T., Endo, N., Mori, H., (1994) J. Biol. Chem., 269, pp. 10205-10208
Winn, M.D., (2011) Acta Cryst., D67, pp. 235-242
Xu, Q., (2010) Acta Cryst., F66, pp. 1354-1364
Zhang, W., Sulea, T., Tao, L., Cui, Q., Purisima, E.O., Vongsamphanh, R., Lachance, P., Ménard, R., (2011) Biochemistry, 50, pp. 4775-4775
B th, D., Schneider, G., Schnell, R., (2011) J. Mol. Biol., 413, pp. 247-260
Chao, M. C., Kieser, K. J., Minami, S., Mavrici, D., Aldridge, B. B., Fortune, S. M., Alber, T., Rubin, E. J., (2013) PLoS Pathog., 9, p. e1003197
Cloos, P. A., Christgau, S., (2002) Matrix Biol., 21, pp. 39-52
Heck, S. D., (1994) Science, 266, pp. 1065-1068
Hett, E. C., Chao, M. C., Deng, L. L., Rubin, E. J., (2008) PLoS Pathog., 4, p. e1000001
Hett, E. C., Rubin, E. J., (2008) Microbiol. Mol. Biol. Rev., 72, pp. 126-156
Kamphuis, I. G., Kalk, K. H., Swarte, M. B., Drenth, J., (1984) J. Mol. Biol., 179, pp. 233-256
Laskowski, R. A., Rullmannn, J. A., MacArthur, M. W., Kaptein, R., Thornton, J. M., (1996) J. Biomol. NMR, 8, pp. 477-486
Layec, S., G rard, J., Legu, V., Chapot-Chartier, M. -P., Courtin, P., Borges, F., Decaris, B., Leblond-Bourget, N., (2009) Mol Microbiol., 71, pp. 1205-1217
Mart nez-Caballero, S., Lee, M., Artola-Recolons, C., Carrasco-L pez, C., Hesek, D., Spink, E., Lastochkin, E., Hermoso, J. A., (2013) J. Am. Chem. Soc., 135, pp. 10318-10321
Meroueh, S. O., Bencze, K. Z., Hesek, D., Lee, M., Fisher, J. F., Stemmler, T. L., Mobashery, S., (2006) Proc. Natl Acad. Sci. USA, 103, pp. 4404-4409
Murshudov, G. N., Skub k, P., Lebedev, A. A., Pannu, N. S., Steiner, R. A., Nicholls, R. A., Winn, M. D., Vagin, A. A., (2011) Acta Cryst., D67, pp. 355-367
Nikitushkin, V. D., Demina, G. R., Shleeva, M. O., Kaprelyants, A. S., (2013) Antonie Van Leeuwenhoek, 103, pp. 37-46
Sheldrick, G. M., (2008) Acta Cryst., A64, pp. 112-122
Storer, A. C., M nard, R., (1994) Methods Enzymol., 244, pp. 486-500
Winn, M. D., (2011) Acta Cryst., D67, pp. 235-242
Mutational and structural study of RipA, a key enzyme in Mycobacterium tuberculosis cell division: Evidence for the l-to-d inversion of configuration of the catalytic cysteine
RipA is a key cysteine protease of Mycobacterium tuberculosis as it is responsible for bacterial daughter-cell separation. Although it is an important target for antimicrobial development, its mechanism of action and its interaction pattern with its substrate are hitherto unknown. By combining crystallo graphic and mutational studies with functional assays and molecular modelling, it is shown that the catalytic activity of the enzyme relies on a Cys-His-Glu triad and the impact of the mutation of each residue of the triad on the structure and function of RipA is analysed. Unexpectedly, the crystallo graphic analyses reveal that mutation of the glutamic acid to alanine results in inversion of the configuration of the catalytic cysteine. The consequent burial of the catalytic cysteine side chain explains the enzyme inactivation upon mutation. These data point to a novel role of the acidic residue often present in the triad of cysteine proteases as a supervisor of cysteine configuration through preservation of the local structural integrity.
RipA is a key cysteine protease of Mycobacterium tuberculosis as it is responsible for bacterial daughter-cell separation. Although it is an important target for antimicrobial development, its mechanism of action and its interaction pattern with its substrate are hitherto unknown. By combining crystallo graphic and mutational studies with functional assays and molecular modelling, it is shown that the catalytic activity of the enzyme relies on a Cys-His-Glu triad and the impact of the mutation of each residue of the triad on the structure and function of RipA is analysed. Unexpectedly, the crystallo graphic analyses reveal that mutation of the glutamic acid to alanine results in inversion of the configuration of the catalytic cysteine. The consequent burial of the catalytic cysteine side chain explains the enzyme inactivation upon mutation. These data point to a novel role of the acidic residue often present in the triad of cysteine proteases as a supervisor of cysteine configuration through preservation of the local structural integrity.
Mutational and structural study of RipA, a key enzyme in Mycobacterium tuberculosis cell division: Evidence for the l-to-d inversion of configuration of the catalytic cysteine
| ▼ Structural and functional studies of virulence factors in Mycobacterium tuberculosis infection |
Mutational and structural study of RipA, a key enzyme in Mycobacterium tuberculosis cell division: Evidence for the l-to-d inversion of configuration of the catalytic cysteine
Other filter: ([btitle,keywords] "Cysteine Protease" O ...
Romano M, Squeglia F, Kramarska E, Barra G, Choi HG, Kim HJ, Ruggiero A, Berisio R
* A Structural View at Vaccine Development against M. tuberculosis (0 views)
Cells (ISSN: 2073-4409linking), 2023 Jan 14; 12 (2 ): N/D-N/D.
Impact Factor:
View Export to BibTeX Export to EndNote
Berisio R, Delogu G
* PGRS domain structures: Doomed to sail the mycomembrane (11 views)
Plos Pathog (ISSN: 1553-7366linking), 2022 Sep 1; 18(9): e1010760-N/D.
Impact Factor: 7.5
View Export to BibTeX Export to EndNote
Squeglia F, Marasco D, Ruggiero A, Testa G, Esposito L, Berisio R
* Structural and Binding Properties of the Active Cell Wall Hydrolase RipA from M. tuberculosis, a Promising Biosensing Molecule for Early Warning Bacterial Detection (5 views)
Curr Med Chem (ISSN: 0929-8673linking), 2022; 29(24): 4282-4292.
Impact Factor: 4.74
View Export to BibTeX Export to EndNote
Kramarska E, Squeglia F, De Maio F, Delogu G, Berisio R
* PE_PGRS33, an Important Virulence Factor of Mycobacterium tuberculosis and Potential Target of Host Humoral Immune Response (19 views)
Cells (ISSN: 2073-4409linking, 2073-4409electronic), 2021 Jan 15; 10(1): N/D-N/D.
Impact Factor: 6.6
View Export to BibTeX Export to EndNote
De Maio F, Berisio R, Manganelli R, Delogu G
* PE_PGRS proteins of Mycobacterium tuberculosis: A specialized molecular task force at the forefront of host-pathogen interaction (102 views)
Virulence, 2020; N/D: 898-915.
Impact Factor: 5.54
View Export to BibTeX Export to EndNote
Squeglia F, Moreira M, Ruggiero A, Berisio R
* The Cell Wall Hydrolytic NlpC/P60 Endopeptidases in Mycobacterial Cytokinesis: A Structural Perspective (218 views)
Cells (ISSN: 2073-4409linking, 2073-4409electronic), 2019 Jun 18; 8(6): N/D-N/D.
Impact Factor: 5.7
View Export to BibTeX Export to EndNote
De Maio F, Squeglia F, Goletti D, Delogu G
* The Mycobacterial HBHA Protein: a Promising Biomarker for Tuberculosis (208 views)
Curr Med Chem (ISSN: 0929-8673linking, 1875-533xelectronic), 2018 Oct 29; 26(11): 2051-2060.
Impact Factor: 3.519
View Export to BibTeX Export to EndNote
Squeglia F, Ruggiero A, Berisio R
* Collagen degradation in tuberculosis pathogenesis: the biochemical consequences of hosting an undesired guest (395 views)
Biochem J (ISSN: 1470-8728electronic, 0264-6021linking, 0264-6021print), 2018 Oct 11; 475(19): 3123-3140.
Impact Factor: 4.897
View Export to BibTeX Export to EndNote
Squeglia F, Ruggiero A, Berisio R
* Chemistry of Peptidoglycan in Mycobacterium tuberculosis Life Cycle: An off-the-wall Balance of Synthesis and Degradation (104 views)
Chemistry (ISSN: 0947-6539linking, 1521-3765electronic), 2018 Feb 21; 24 (11 ): 2533-2546.
Impact Factor: 5.476
View Export to BibTeX Export to EndNote
Squeglia F, Ruggiero A, De Simone A, Berisio R
* A structural overview of mycobacterial adhesins: Key biomarkers for diagnostics and therapeutics (258 views)
Protein Sci (ISSN: 1469-896xelectronic, 0961-8368linking), 2018 Feb; 27(2): 369-380.
Impact Factor: 2.523
View Export to BibTeX Export to EndNote
Squeglia F, Ruggiero A, Berisio R
* Chemistry of Peptidoglycan in Mycobacterium tuberculosis life style: an off-the-wall balance of synthesis and degradation (479 views) (PDF 222 views)
Chemistry (ISSN: 1521-3765electronic, 0947-6539linking), 2018; 24: 2533-2546.
Impact Factor: 5.317
View Export to BibTeX Export to EndNote
Berisio R, Squeglia F, Ruggiero A
* Editorial: Molecular Scenarios Behind Infectious Diseases (390 views)
Curr Med Chem (ISSN: 0929-8673, 1875-533x, 1875-533xelectronic), 2017 Nov 24; 24(36): 3939-3941.
Impact Factor: 3.469
View Export to BibTeX Export to EndNote
Squeglia F, Romano M, Ruggiero A, Berisio R
* Molecular players in Tuberculosis drug development: another break in the cell wall (321 views)
Curr Med Chem (ISSN: 0929-8673, 1875-533x, 1875-533xelectronic), 2017 Nov 24; 24(36): 3954-3969.
Impact Factor: 3.469
View Export to BibTeX Export to EndNote
Ruggiero A, Squeglia F, Romano M, Vitagliano L, De Simone A, Berisio R
* Structure and dynamics of the multi-domain resuscitation promoting factor RpfB from Mycobacterium tuberculosis (359 views)
J Biomol Struct Dyn (ISSN: 0739-1102, 1538-0254electronic, 0739-1102linking), 2017 May; 35(6): 1322-1330.
Impact Factor: 3.107
View Export to BibTeX Export to EndNote
Ruggiero A, Squeglia F, Romano M, Vitagliano L, De Simone A, Berisio R
* The structure of Resuscitation promoting factor B from M. tuberculosis reveals unexpected ubiquitin-like domains (328 views)
Bba-Gen Subjects (ISSN: 0005-2728, 0006-3002print, 1570-9639print), 2015 Nov 5; 1860(2): 445-451.
Impact Factor: 4.864
View Export to BibTeX Export to EndNote
Romano M, Squeglia F, Berisio R
* Structure and Function of RNase AS, a Novel Virulence Factor from Mycobacterium Tuberculosis (405 views)
Curr Med Chem (ISSN: 0929-8673, 1875-533x, 1875-533xelectronic), 2015 Apr 17; 22(14): 1745-1756.
Impact Factor: 3.455
View Export to BibTeX Export to EndNote
Squeglia F, Ruggiero A, Berisio R
* Exit from mycobacterial dormancy: a structural perspective (521 views)
Curr Med Chem (ISSN: 0929-8673, 1875-533x, 1875-533xelectronic), 2015 Feb 9; 22(14): 1698-1709.
Impact Factor: 3.455
View Export to BibTeX Export to EndNote
Calvanese L, Falcigno L, Maglione C, Marasco D, Ruggiero A, Squeglia F, Berisio R, D'Auria G
* Structural and binding properties of the PASTA domain of PonA2, a key penicillin binding protein from Mycobacterium tuberculosis (478 views)
Biopolymers (ISSN: 0006-3525, 0006-6352, 0006-3525print), 2014 Jul; 101(7): 712-719.
Impact Factor: 2.385
View Export to BibTeX Export to EndNote
Romano M, Van De Weerd R, Brouwer FCC, Roviello GN, Lacroix R, Sparrius M, Van Den Brink-van Stempvoort G, Maaskant JJ, Van Der Sar AM, Appelmelk BJ, Geurtsen JJ, Berisio R
* Structure and function of RNase AS, a polyadenylate-specific exoribonuclease affecting mycobacterial virulence in vivo (375 views)
Structure (ISSN: 0969-2126, 1878-4186, 0969-2126linking), 2014 May 6; 22(5): 719-730.
Impact Factor: 5.618
View Export to BibTeX Export to EndNote
Correale S, Ruggiero A, Capparelli R, Pedone E, Berisio R
* Structures of free and inhibited forms of the L, D-transpeptidase LdtMt1 from Mycobacterium tuberculosis (402 views)
Acta Crystallogr D Biol Crystallogr (ISSN: 0907-4449, 0907-4449linking), 2013 Sep; 69(Pt9): 1697-1706.
Impact Factor: 7.232
View Export to BibTeX Export to EndNote
Squeglia F, Romano M, Ruggiero A, Vitagliano L, De Simone A, Berisio R
* Carbohydrate recognition by RpfB from mycobacterium tuberculosis unveiled by crystallographic and molecular dynamics analyses (520 views)
Biophysical Journal (ISSN: 0006-3495, 1542-0086), 2013 Jun 4; 104(11): 2530-2539.
Impact Factor: 3.832
View Export to BibTeX Export to EndNote
Correale S, Ruggiero A, Pedone E, Berisio R
* Expression, purification, crystallization and preliminary X-ray crystallographic analysis of the l, d-transpeptidase LdtMt1 from Mycobacterium tuberculosis (430 views)
Nucleosides Nucleotides Nucleic Acids (ISSN: 1744-3091, 1744-4309), 2013 Mar 1; 69(3): 253-256.
Impact Factor: 0.568
View Export to BibTeX Export to EndNote
Ruggiero A, Marchant J, Squeglia F, Makarov V, De Simone A, Berisio R
* Molecular determinants of inactivation of the resuscitation promoting factor B from Mycobacterium tuberculosis (507 views)
J Biomol Struct Dyn (ISSN: 0739-1102, 1538-0254electronic, 0739-1102linking), 2013 Feb 1; 31(2): 195-205.
Impact Factor: 2.983
View Export to BibTeX Export to EndNote
Kaprelyants AS, Mukamolova GV, Ruggiero A, Makarov VA, Demina GR, Shleeva MO, Potapov VD, Shramko PA
* Resuscitation-promoting Factors (Rpf): In Search of Inhibitors (503 views)
Protein Pept Lett (ISSN: 0929-8665, 1875-5305), 2012 Oct; 19(10): 1026-1034.
Impact Factor: 1.994
View Export to BibTeX Export to EndNote
Esposito C, Cantisani M, D'Auria G, Falcigno L, Pedone E, Galdiero S, Berisio R
* Mapping key interactions in the dimerization process of HBHA from Mycobacterium tuberculosis, insights into bacterial agglutination (476 views)
Febs Lett (ISSN: 0014-5793, 0014-5793print, 1873-3468electronic), 2012 Mar 23; 586(6): 659-667.
Impact Factor: 3.582
View Export to BibTeX Export to EndNote
Esposito C, Marasco D, Delogu G, Pedone E, Berisio R
* Heparin-binding hemagglutinin HBHA from Mycobacterium tuberculosis affects actin polymerisation (362 views)
Biochem Biophys Res Commun (ISSN: 0006-291x), 2011 Jul 1; 410(2): 339-344.
Impact Factor: 2.484
View Export to BibTeX Export to EndNote
Ruggiero A, Squeglia F, Pirone L, Correale S, Berisio R
* Expression, purification, crystallization and preliminary X-ray crystallographic analysis of a major fragment of the resuscitation-promoting factor RpfB from Mycobacterium tuberculosis (408 views)
Nucleosides Nucleotides Nucleic Acids (ISSN: 1744-3091, 1744-4309), 2011 Jan 1; 67(1): 164-168.
Impact Factor: 0.506
View Export to BibTeX Export to EndNote
D'Auria G, Esposito C, Falcigno L, Calvanese L, Iaccarino E, Ruggiero A, Pedone C, Pedone E, Berisio R
* Dynamical properties of cold shock protein A from Mycobacterium tuberculosis (321 views)
Biochem Biophys Res Commun (ISSN: 0006-291x, 1090-2104, 1090-2104electronic), 2010 Nov 26; 402(4): 693-698.
Impact Factor: 2.595
View Export to BibTeX Export to EndNote
Ruggiero A, Marasco D, Squeglia F, Soldini S, Pedone E, Pedone C, Berisio R
* Structure and functional regulation of RipA, a mycobacterial enzyme essential for daughter cell separation (425 views)
Structure (ISSN: 0969-2126, 1878-4186, 0969-2126linking), 2010 Sep 8; 18(9): 1184-1190.
Impact Factor: 6.337
View Export to BibTeX Export to EndNote
Esposito C, Carullo C, Pedone E, Graziano G, Del Vecchio P, Berisio R
* Dimerisation and structural integrity of Heparin Binding Hemagglutinin A from Mycobacterium tuberculosis: Implications for bacterial agglutination (495 views)
Febs Lett (ISSN: 0014-5793, 0014-5793print, 0014-5793linking), 2010 Mar 19; 584(6): 1091-1096.
Impact Factor: 3.601
View Export to BibTeX Export to EndNote
Ruggiero A, Squeglia F, Esposito C, Marasco D, Pedone E, Pedone C, Berisio R
* Expression, purification, crystallization and preliminary X-ray crystallographic analysis of the resuscitation promoting factor interacting protein RipA from M. tuberculosis (348 views)
Protein Pept Lett (ISSN: 0929-8665, 1875-5305), 2010 Jan; 17(1): 70-73.
Impact Factor: 1.849
View Export to BibTeX Export to EndNote
Ruggiero A, Tizzano B, Pedone E, Pedone C, Wilmanns M, Berisio R
* Crystal Structure of the Resuscitation-Promoting Factor (Delta DUF)RpfB from M. tuberculosis (598 views)
J Mol Biol (ISSN: 0022-2836, 1089-8638, 1089-8638electronic), 2009 Jan 9; 385(1): 153-162.
Impact Factor: 3.871
View Export to BibTeX Export to EndNote
Esposito C, Pethoukov MV, Svergun DI, Ruggiero A, Pedone C, Pedone E, Berisio R
* Evidence for an elongated dimeric structure of heparin-binding hemagglutinin from Mycobacterium tuberculosis (475 views)
J Bacteriol Journal Of Bacteriology (ISSN: 0021-9193, 1098-5530), 2008 Jul; 190(13): 4749-4753.
Impact Factor: 3.636
View Export to BibTeX Export to EndNote
Ruggiero A, Tizzano B, Geerlof A, Pedone E, Pedone C, Wilmanns M, Berisio R
* Expression, purification, crystallization and preliminary X-ray crystallographic analysis of a resuscitation-promoting factor from Mycobacterium tuberculosis (389 views)
Nucleosides Nucleotides Nucleic Acids (ISSN: 1744-3091, 1744-4309), 2007 Oct 1; 63(10): 870-873.
Impact Factor: 0.645
View Export to BibTeX Export to EndNote
* A Structural View at Vaccine Development against M. tuberculosis (0 views)
Cells (ISSN: 2073-4409linking), 2023 Jan 14; 12 (2 ): N/D-N/D.
Impact Factor:
View Export to BibTeX Export to EndNote
Berisio R, Delogu G
* PGRS domain structures: Doomed to sail the mycomembrane (11 views)
Plos Pathog (ISSN: 1553-7366linking), 2022 Sep 1; 18(9): e1010760-N/D.
Impact Factor: 7.5
View Export to BibTeX Export to EndNote
Squeglia F, Marasco D, Ruggiero A, Testa G, Esposito L, Berisio R
* Structural and Binding Properties of the Active Cell Wall Hydrolase RipA from M. tuberculosis, a Promising Biosensing Molecule for Early Warning Bacterial Detection (5 views)
Curr Med Chem (ISSN: 0929-8673linking), 2022; 29(24): 4282-4292.
Impact Factor: 4.74
View Export to BibTeX Export to EndNote
Kramarska E, Squeglia F, De Maio F, Delogu G, Berisio R
* PE_PGRS33, an Important Virulence Factor of Mycobacterium tuberculosis and Potential Target of Host Humoral Immune Response (19 views)
Cells (ISSN: 2073-4409linking, 2073-4409electronic), 2021 Jan 15; 10(1): N/D-N/D.
Impact Factor: 6.6
View Export to BibTeX Export to EndNote
De Maio F, Berisio R, Manganelli R, Delogu G
* PE_PGRS proteins of Mycobacterium tuberculosis: A specialized molecular task force at the forefront of host-pathogen interaction (102 views)
Virulence, 2020; N/D: 898-915.
Impact Factor: 5.54
View Export to BibTeX Export to EndNote
Squeglia F, Moreira M, Ruggiero A, Berisio R
* The Cell Wall Hydrolytic NlpC/P60 Endopeptidases in Mycobacterial Cytokinesis: A Structural Perspective (218 views)
Cells (ISSN: 2073-4409linking, 2073-4409electronic), 2019 Jun 18; 8(6): N/D-N/D.
Impact Factor: 5.7
View Export to BibTeX Export to EndNote
De Maio F, Squeglia F, Goletti D, Delogu G
* The Mycobacterial HBHA Protein: a Promising Biomarker for Tuberculosis (208 views)
Curr Med Chem (ISSN: 0929-8673linking, 1875-533xelectronic), 2018 Oct 29; 26(11): 2051-2060.
Impact Factor: 3.519
View Export to BibTeX Export to EndNote
Squeglia F, Ruggiero A, Berisio R
* Collagen degradation in tuberculosis pathogenesis: the biochemical consequences of hosting an undesired guest (395 views)
Biochem J (ISSN: 1470-8728electronic, 0264-6021linking, 0264-6021print), 2018 Oct 11; 475(19): 3123-3140.
Impact Factor: 4.897
View Export to BibTeX Export to EndNote
Squeglia F, Ruggiero A, Berisio R
* Chemistry of Peptidoglycan in Mycobacterium tuberculosis Life Cycle: An off-the-wall Balance of Synthesis and Degradation (104 views)
Chemistry (ISSN: 0947-6539linking, 1521-3765electronic), 2018 Feb 21; 24 (11 ): 2533-2546.
Impact Factor: 5.476
View Export to BibTeX Export to EndNote
Squeglia F, Ruggiero A, De Simone A, Berisio R
* A structural overview of mycobacterial adhesins: Key biomarkers for diagnostics and therapeutics (258 views)
Protein Sci (ISSN: 1469-896xelectronic, 0961-8368linking), 2018 Feb; 27(2): 369-380.
Impact Factor: 2.523
View Export to BibTeX Export to EndNote
Squeglia F, Ruggiero A, Berisio R
* Chemistry of Peptidoglycan in Mycobacterium tuberculosis life style: an off-the-wall balance of synthesis and degradation (479 views) (PDF 222 views)
Chemistry (ISSN: 1521-3765electronic, 0947-6539linking), 2018; 24: 2533-2546.
Impact Factor: 5.317
View Export to BibTeX Export to EndNote
Berisio R, Squeglia F, Ruggiero A
* Editorial: Molecular Scenarios Behind Infectious Diseases (390 views)
Curr Med Chem (ISSN: 0929-8673, 1875-533x, 1875-533xelectronic), 2017 Nov 24; 24(36): 3939-3941.
Impact Factor: 3.469
View Export to BibTeX Export to EndNote
Squeglia F, Romano M, Ruggiero A, Berisio R
* Molecular players in Tuberculosis drug development: another break in the cell wall (321 views)
Curr Med Chem (ISSN: 0929-8673, 1875-533x, 1875-533xelectronic), 2017 Nov 24; 24(36): 3954-3969.
Impact Factor: 3.469
View Export to BibTeX Export to EndNote
Ruggiero A, Squeglia F, Romano M, Vitagliano L, De Simone A, Berisio R
* Structure and dynamics of the multi-domain resuscitation promoting factor RpfB from Mycobacterium tuberculosis (359 views)
J Biomol Struct Dyn (ISSN: 0739-1102, 1538-0254electronic, 0739-1102linking), 2017 May; 35(6): 1322-1330.
Impact Factor: 3.107
View Export to BibTeX Export to EndNote
Ruggiero A, Squeglia F, Romano M, Vitagliano L, De Simone A, Berisio R
* The structure of Resuscitation promoting factor B from M. tuberculosis reveals unexpected ubiquitin-like domains (328 views)
Bba-Gen Subjects (ISSN: 0005-2728, 0006-3002print, 1570-9639print), 2015 Nov 5; 1860(2): 445-451.
Impact Factor: 4.864
View Export to BibTeX Export to EndNote
Romano M, Squeglia F, Berisio R
* Structure and Function of RNase AS, a Novel Virulence Factor from Mycobacterium Tuberculosis (405 views)
Curr Med Chem (ISSN: 0929-8673, 1875-533x, 1875-533xelectronic), 2015 Apr 17; 22(14): 1745-1756.
Impact Factor: 3.455
View Export to BibTeX Export to EndNote
Squeglia F, Ruggiero A, Berisio R
* Exit from mycobacterial dormancy: a structural perspective (521 views)
Curr Med Chem (ISSN: 0929-8673, 1875-533x, 1875-533xelectronic), 2015 Feb 9; 22(14): 1698-1709.
Impact Factor: 3.455
View Export to BibTeX Export to EndNote
Calvanese L, Falcigno L, Maglione C, Marasco D, Ruggiero A, Squeglia F, Berisio R, D'Auria G
* Structural and binding properties of the PASTA domain of PonA2, a key penicillin binding protein from Mycobacterium tuberculosis (478 views)
Biopolymers (ISSN: 0006-3525, 0006-6352, 0006-3525print), 2014 Jul; 101(7): 712-719.
Impact Factor: 2.385
View Export to BibTeX Export to EndNote
Romano M, Van De Weerd R, Brouwer FCC, Roviello GN, Lacroix R, Sparrius M, Van Den Brink-van Stempvoort G, Maaskant JJ, Van Der Sar AM, Appelmelk BJ, Geurtsen JJ, Berisio R
* Structure and function of RNase AS, a polyadenylate-specific exoribonuclease affecting mycobacterial virulence in vivo (375 views)
Structure (ISSN: 0969-2126, 1878-4186, 0969-2126linking), 2014 May 6; 22(5): 719-730.
Impact Factor: 5.618
View Export to BibTeX Export to EndNote
Correale S, Ruggiero A, Capparelli R, Pedone E, Berisio R
* Structures of free and inhibited forms of the L, D-transpeptidase LdtMt1 from Mycobacterium tuberculosis (402 views)
Acta Crystallogr D Biol Crystallogr (ISSN: 0907-4449, 0907-4449linking), 2013 Sep; 69(Pt9): 1697-1706.
Impact Factor: 7.232
View Export to BibTeX Export to EndNote
Squeglia F, Romano M, Ruggiero A, Vitagliano L, De Simone A, Berisio R
* Carbohydrate recognition by RpfB from mycobacterium tuberculosis unveiled by crystallographic and molecular dynamics analyses (520 views)
Biophysical Journal (ISSN: 0006-3495, 1542-0086), 2013 Jun 4; 104(11): 2530-2539.
Impact Factor: 3.832
View Export to BibTeX Export to EndNote
Correale S, Ruggiero A, Pedone E, Berisio R
* Expression, purification, crystallization and preliminary X-ray crystallographic analysis of the l, d-transpeptidase LdtMt1 from Mycobacterium tuberculosis (430 views)
Nucleosides Nucleotides Nucleic Acids (ISSN: 1744-3091, 1744-4309), 2013 Mar 1; 69(3): 253-256.
Impact Factor: 0.568
View Export to BibTeX Export to EndNote
Ruggiero A, Marchant J, Squeglia F, Makarov V, De Simone A, Berisio R
* Molecular determinants of inactivation of the resuscitation promoting factor B from Mycobacterium tuberculosis (507 views)
J Biomol Struct Dyn (ISSN: 0739-1102, 1538-0254electronic, 0739-1102linking), 2013 Feb 1; 31(2): 195-205.
Impact Factor: 2.983
View Export to BibTeX Export to EndNote
Kaprelyants AS, Mukamolova GV, Ruggiero A, Makarov VA, Demina GR, Shleeva MO, Potapov VD, Shramko PA
* Resuscitation-promoting Factors (Rpf): In Search of Inhibitors (503 views)
Protein Pept Lett (ISSN: 0929-8665, 1875-5305), 2012 Oct; 19(10): 1026-1034.
Impact Factor: 1.994
View Export to BibTeX Export to EndNote
Esposito C, Cantisani M, D'Auria G, Falcigno L, Pedone E, Galdiero S, Berisio R
* Mapping key interactions in the dimerization process of HBHA from Mycobacterium tuberculosis, insights into bacterial agglutination (476 views)
Febs Lett (ISSN: 0014-5793, 0014-5793print, 1873-3468electronic), 2012 Mar 23; 586(6): 659-667.
Impact Factor: 3.582
View Export to BibTeX Export to EndNote
Esposito C, Marasco D, Delogu G, Pedone E, Berisio R
* Heparin-binding hemagglutinin HBHA from Mycobacterium tuberculosis affects actin polymerisation (362 views)
Biochem Biophys Res Commun (ISSN: 0006-291x), 2011 Jul 1; 410(2): 339-344.
Impact Factor: 2.484
View Export to BibTeX Export to EndNote
Ruggiero A, Squeglia F, Pirone L, Correale S, Berisio R
* Expression, purification, crystallization and preliminary X-ray crystallographic analysis of a major fragment of the resuscitation-promoting factor RpfB from Mycobacterium tuberculosis (408 views)
Nucleosides Nucleotides Nucleic Acids (ISSN: 1744-3091, 1744-4309), 2011 Jan 1; 67(1): 164-168.
Impact Factor: 0.506
View Export to BibTeX Export to EndNote
D'Auria G, Esposito C, Falcigno L, Calvanese L, Iaccarino E, Ruggiero A, Pedone C, Pedone E, Berisio R
* Dynamical properties of cold shock protein A from Mycobacterium tuberculosis (321 views)
Biochem Biophys Res Commun (ISSN: 0006-291x, 1090-2104, 1090-2104electronic), 2010 Nov 26; 402(4): 693-698.
Impact Factor: 2.595
View Export to BibTeX Export to EndNote
Ruggiero A, Marasco D, Squeglia F, Soldini S, Pedone E, Pedone C, Berisio R
* Structure and functional regulation of RipA, a mycobacterial enzyme essential for daughter cell separation (425 views)
Structure (ISSN: 0969-2126, 1878-4186, 0969-2126linking), 2010 Sep 8; 18(9): 1184-1190.
Impact Factor: 6.337
View Export to BibTeX Export to EndNote
Esposito C, Carullo C, Pedone E, Graziano G, Del Vecchio P, Berisio R
* Dimerisation and structural integrity of Heparin Binding Hemagglutinin A from Mycobacterium tuberculosis: Implications for bacterial agglutination (495 views)
Febs Lett (ISSN: 0014-5793, 0014-5793print, 0014-5793linking), 2010 Mar 19; 584(6): 1091-1096.
Impact Factor: 3.601
View Export to BibTeX Export to EndNote
Ruggiero A, Squeglia F, Esposito C, Marasco D, Pedone E, Pedone C, Berisio R
* Expression, purification, crystallization and preliminary X-ray crystallographic analysis of the resuscitation promoting factor interacting protein RipA from M. tuberculosis (348 views)
Protein Pept Lett (ISSN: 0929-8665, 1875-5305), 2010 Jan; 17(1): 70-73.
Impact Factor: 1.849
View Export to BibTeX Export to EndNote
Ruggiero A, Tizzano B, Pedone E, Pedone C, Wilmanns M, Berisio R
* Crystal Structure of the Resuscitation-Promoting Factor (Delta DUF)RpfB from M. tuberculosis (598 views)
J Mol Biol (ISSN: 0022-2836, 1089-8638, 1089-8638electronic), 2009 Jan 9; 385(1): 153-162.
Impact Factor: 3.871
View Export to BibTeX Export to EndNote
Esposito C, Pethoukov MV, Svergun DI, Ruggiero A, Pedone C, Pedone E, Berisio R
* Evidence for an elongated dimeric structure of heparin-binding hemagglutinin from Mycobacterium tuberculosis (475 views)
J Bacteriol Journal Of Bacteriology (ISSN: 0021-9193, 1098-5530), 2008 Jul; 190(13): 4749-4753.
Impact Factor: 3.636
View Export to BibTeX Export to EndNote
Ruggiero A, Tizzano B, Geerlof A, Pedone E, Pedone C, Wilmanns M, Berisio R
* Expression, purification, crystallization and preliminary X-ray crystallographic analysis of a resuscitation-promoting factor from Mycobacterium tuberculosis (389 views)
Nucleosides Nucleotides Nucleic Acids (ISSN: 1744-3091, 1744-4309), 2007 Oct 1; 63(10): 870-873.
Impact Factor: 0.645
View Export to BibTeX Export to EndNote
34 Records (34 excluding Abstracts).
Total impact factor: 127.349 (127.349 excluding Abstracts).
Total 5 year impact factor: 127.554 (127.554 excluding Abstracts).
Total impact factor: 127.349 (127.349 excluding Abstracts).
Total 5 year impact factor: 127.554 (127.554 excluding Abstracts).
487 views. Last view on Monday 24 April 2023, 6:29:03
ibb.cnr.it
