Structures of free and inhibited forms of the L, D-transpeptidase LdtMt1 from Mycobacterium tuberculosis(542 views) Correale S, Ruggiero A, Capparelli R, Pedone E, Berisio R
Keywords: Cell Wall, D-Transpeptidases, Peptidoglycan, Tuberculosis, Antiinfective Agent, Bacterial Protein, Imipenem, Peptidyltransferase, Recombinant Protein, Article, Biosynthesis, Chemistry, Crystallization, Drug Antagonism, Drug Effect, Enzyme Active Site, Enzymology, Genetics, Isolation And Purification, Metabolism, Multidrug Resistant Tuberculosis, Mycobacterium Tuberculosis, Protein Binding, X Ray Crystallography, Anti-Bacterial Agents, Catalytic Domain, X-Ray, Peptidyl Transferases, Multidrug-Resistant, Anti-Bacterial Agents Chemistry, Bacterial Proteins Antagonists, Inhibitors Chemistry Genetics, Imipenem Pharmacology Therapeutic Use, Mycobacterium Tuberculosis Drug Effects Enzymology, Peptidyl Transferases Antagonists, Recombinant Proteins Biosynthesis Chemistry Isolation, Multidrug-Resistant Drug Therapy Enzymology Metabolism,
Affiliations: *** IBB - CNR ***
Institute of Biostructures and Bioimaging, CNR, Via Mezzocannone 16, 80134 Napoli, Italy
Kedrion S.p.A, 80029 S. Antimo (Napoli), Italy
Department of Soil, Plant, Environmental and Animal Production Sciences, School of Biotechnological Sciences, University of Naples 'Federico II', Portici, Italy
Kedrion S. p. A, 80029 S. Antimo (Napoli), Italy
References: Not available.
Structures of free and inhibited forms of the L, D-transpeptidase LdtMt1 from Mycobacterium tuberculosis
The modelling of peptidoglycan is responsible for key cellular processes in Mycobacterium tuberculosis such as cell growth, division and resuscitation from dormancy. The structure of M. tuberculosis peptidoglycan is atypical since it contains a majority of 3,3 cross-links synthesized by L,D-transpeptidases that replace the 4,3 cross-links formed by the D,D-transpeptidase activity of classical penicillin-binding proteins. Carbapenems inactivate these L,D-transpeptidases and in combination with clavulanic acid are bactericidal against extensively drug-resistant M. tuberculosis. Here, crystal structures of the L,D-transpeptidase LdtMt1 from M. tuberculosis in a ligand-free form and in complex with the carbapenem imipenem are reported. Elucidation of the structural features of LdtMt1 unveils analogies and differences between the two key transpeptidases of M. tuberculosis: LdtMt1 and LdtMt2. In addition, the structure of imipenem-inactivated LdtMt1 provides a detailed structural view of the interactions between a carbapenem drug and LdtMt1. By providing the key interactions in the binding of carbapenem to LdtMt1, this work will facilitate structure-guided discovery of L,D-transpeptidase inhibitors as novel antitubercular agents against drug-resistant M. tuberculosis.
Structures of free and inhibited forms of the L, D-transpeptidase LdtMt1 from Mycobacterium tuberculosis