Reaction of Hg2+ Insertion into Cysteine Pairs Within Bovine Insulin Crystals Followed via Raman Spectroscopy (372 views)
J Solution Chem (ISSN: 0095-9782), 2014 Feb; 43(1): 135-143.
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Paper type: Journal Article,
Impact factor: 1.177, 5-year impact factor: 1.303
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84894429683&partnerID=40&md5=ff5bdb8fd6dd1e495720a24cbba8d8d2
Keywords: Disulfide Bridges, Insulin, Mercury Binding, Protein Crystallization, Raman Spectroscopy,
Affiliations:
*** IBB - CNR ***
Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario di Monte sant'Angelo, Via Cintia, 80126 Naples, Italy
CNR, Institute of Biostructures and Bioimages, Naples, Italy
Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Naples, Italy
References:
Not available.
J Solution Chem (ISSN: 0095-9782), 2014 Feb; 43(1): 135-143.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 1.177, 5-year impact factor: 1.303
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84894429683&partnerID=40&md5=ff5bdb8fd6dd1e495720a24cbba8d8d2
Keywords: Disulfide Bridges, Insulin, Mercury Binding, Protein Crystallization, Raman Spectroscopy,
Affiliations:
*** IBB - CNR ***
Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario di Monte sant'Angelo, Via Cintia, 80126 Naples, Italy
CNR, Institute of Biostructures and Bioimages, Naples, Italy
Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Naples, Italy
References:
Not available.
Reaction of Hg2+ Insertion into Cysteine Pairs Within Bovine Insulin Crystals Followed via Raman Spectroscopy
Chemical modifications of protein crystals may be achieved via soaking of reactants from their precipitating solution, through the solvent channel, into the protein matrix. We describe a Raman microscopy approach to follow mercury insertion into cysteine pairs within protein single crystals, via soaking in an aqueous Hg2+ solution. The method has been developed using bovine insulin as the model system. Applying an efficient mercuration protocol, consisting of a first step of disulphide bridge TCEP-induced reduction within the crystal, followed by overnight reaction with a HgCl2 solution, we obtained Hg-derivative crystals. Raman spectra collected on these derivative crystals, kept in the mother liquor, reveal a characteristic Raman band at 335 cm(-1), which has been assigned to a -S-Hg-S- bridge. The analysis provides Raman-based markers of mercury binding to cysteines, and thus of mercury intoxication.
Chemical modifications of protein crystals may be achieved via soaking of reactants from their precipitating solution, through the solvent channel, into the protein matrix. We describe a Raman microscopy approach to follow mercury insertion into cysteine pairs within protein single crystals, via soaking in an aqueous Hg2+ solution. The method has been developed using bovine insulin as the model system. Applying an efficient mercuration protocol, consisting of a first step of disulphide bridge TCEP-induced reduction within the crystal, followed by overnight reaction with a HgCl2 solution, we obtained Hg-derivative crystals. Raman spectra collected on these derivative crystals, kept in the mother liquor, reveal a characteristic Raman band at 335 cm(-1), which has been assigned to a -S-Hg-S- bridge. The analysis provides Raman-based markers of mercury binding to cysteines, and thus of mercury intoxication.
Reaction of Hg2+ Insertion into Cysteine Pairs Within Bovine Insulin Crystals Followed via Raman Spectroscopy
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Reaction of Hg2+ Insertion into Cysteine Pairs Within Bovine Insulin Crystals Followed via Raman Spectroscopy
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View Export to BibTeX Export to EndNote
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View Export to BibTeX Export to EndNote
Vergara A, Lorber B, Zagari A, Giege R
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View Export to BibTeX Export to EndNote
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View Export to BibTeX Export to EndNote
Carotenuto L, Berisio R, Piccolo C, Vitagliano L, Zagari A
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Impact Factor: 1.283
View Export to BibTeX Export to EndNote
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Impact Factor: 1.492
View Export to BibTeX Export to EndNote
Esposito L, Sica F, Sorrentino G, Berisio R, Carotenuto L, Giordano A, Raia CA, Rossi M, Lamzin VS, Wilson KS, Zagari A
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Impact Factor: 2.244
View Export to BibTeX Export to EndNote
16 Records (16 excluding Abstracts).
Total impact factor: 45.005 (45.005 excluding Abstracts).
Total 5 year impact factor: 57.897 (57.897 excluding Abstracts).
Total impact factor: 45.005 (45.005 excluding Abstracts).
Total 5 year impact factor: 57.897 (57.897 excluding Abstracts).
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