Lysozyme mutual diffusion in solutions crowded by poly(ethylene glycol)
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Lysozyme mutual diffusion in solutions crowded by poly(ethylene glycol) (351 views)

Vergara A, Capuano F, Paduano L, Sartorio R

Macromolecules (ISSN: 0024-9297), 2006; 39(13): 4500-4506.

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Paper type: Journal Article,

Impact factor: 4.277, 5-year impact factor: 5.531

Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-33746353062&partnerID=40&md5=3e16f3929d15ad3fb41d8903bc083064

Keywords: Diffusion, Gravitational Effects, Interferometry, Proteins, Solutions, Ternary Systems, Gravitational Stability, Lysozyme, Macromolecular Crowding, Mutual Diffusion, Polyethylene Glycols,

Affiliations:
*** IBB - CNR ***

Department of Chemistry, University of Naples Federico II, Complesso Monte S. Angelo, Via Cinthia, 80126 Naples, Italy
Istituto di Biostrutture e Bioimmagini, CNR, Via Mezzocannone, 16, 80134, Naples, Italy


References:
Not available.
Lysozyme mutual diffusion in solutions crowded by poly(ethylene glycol)

Mutual diffusion in a multicomponent system at high level of macromolecular crowding has been investigated for a solution containing a protein and a polymeric precipitant. Specifically, precise mutual diffusion coefficients for the ternary system lysozyme (component 1)-PEG2000 (component 2)-water (component 0) up to 25% in volume of PEG have been determined via Gouy interferometry. Experimental diffusion data, Dij, are compared to those predicted by two semiemprirical equations, which are discussed according to the physics behind them. Dij values are used to analyze the gravitational stability of the protein-polymer aqueous solution, and, in combination with the Onsager reciprocal relations, to evaluate the lysozyme salting out coefficient for addition of PEG (μ12). Experimental μ12 are in good agreement with those predicted by Lekkerkerker's equation. All the results have been compared to those corresponding, previously determined, for a companion system containing a PEG sample at a different molecular weight, PEG400. The influence of PEG-induced crowding on proteins in physiological or in crystallization media has been discussed. © 2006 American Chemical Society.
Lysozyme mutual diffusion in solutions crowded by poly(ethylene glycol)

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Lysozyme mutual diffusion in solutions crowded by poly(ethylene glycol)

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6 Records (6 excluding Abstracts).
Total impact factor: 11.828 (11.828 excluding Abstracts).
Total 5 year impact factor: 11.296 (11.296 excluding Abstracts).

Last modified by Marco Comerci on Sunday 12 July 2020, 13:15:07
351 views. Last view on Monday 02 December 2024, 18:21:02