A Blood Test for the Diagnosis of Multiple Sclerosis(29 views) Giuliano P, La Rosa G, Capozzi S, Cassano E, Damiano S, Habetswallner F, Iodice R, Marra M, Pavone LM, Quarantelli M, Vitelli G, Santillo M, Paternò R
Int J Mol Sci (ISSN: 1422-0067linking), 2024 Jan 30; 25
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PRINDEX S.r.l., Via Cuma 28, 80132 Naples, Italy.
Dipartimento di Medicina Clinica e Chirurgia, Università di Napoli Federico II, Via Pansini 5, 80131 Naples, Italy.
Dipartimento di Neuroscienze, Scienze Riproduttive ed Odontostomatologiche, Università di Napoli Federico II, Via Pansini 5, 80131 Napoli, Italy.
UOC Neurofisiopatologia, AORN Antonio Cardarelli, Via Cardarelli 9, 80131 Naples, Italy.
Biostructure and Bioimaging Institute, Consiglio Nazionale delle Ricerche (CNR), Via De Amicis 95, 80145 Naples, Italy.
References: Not available.
A Blood Test for the Diagnosis of Multiple Sclerosis
Multiple sclerosis (MS) is an autoimmune chronic disease characterized by inflammation and demyelination of the central nervous system (CNS). Despite numerous studies conducted, valid biomarkers enabling a definitive diagnosis of MS are not yet available. The aim of our study was to identify a marker from a blood sample to ease the diagnosis of MS. In this study, since there is evidence connecting the serotonin pathway to MS, we used an ELISA (Enzyme-Linked Immunosorbent Assay) to detect serum MS-specific auto-antibodies (auto-Ab) against the extracellular loop 1 (ECL-1) of the 5-hydroxytryptamine (5-HT) receptor subtype 2A (5-HT2A). We utilized an ELISA format employing poly-D-lysine as a pre-coating agent. The binding of 208 serum samples from controls, both healthy and pathological, and of 104 serum samples from relapsing-remitting MS (RRMS) patients was tested. We observed that the serum-binding activity in control cohort sera, including those with autoimmune and neurological diseases, was ten times lower compared to the RRMS patient cohort (p = 1.2 × 10(-47)), with a sensitivity and a specificity of 98% and 100%, respectively. These results show that in the serum of patients with MS there are auto-Ab against the serotonin receptor type 2A which can be successfully used in the diagnosis of MS due to their high sensitivity and specificity.
A Blood Test for the Diagnosis of Multiple Sclerosis
Bogdanovich S, Kim Y, Mizutani T, Yasuma R, Tudisco L, Cicatiello V, Bastos-carvalho A, Kerur N, Hirano Y, Baffi JZ, Tarallo V, Li S, Yasuma T, Arpitha P, Fowler BJ, Wright CB, Apicella I, Greco A, Brunetti A, Ruvo M, Sandomenico A, Nozaki M, Ijima R, Kaneko H, Ogura Y, Terasaki H, Ambati BK, Leusen JH, Langdon WY, Clark MR, Armour KL, Bruhns P, Verbeek JS, Gelfand BD, De Falco S, Ambati J * Human IgG1 antibodies suppress angiogenesis in a target-independent manner(652 views) Signal Transduct Target Ther (ISSN: 2059-3635print), 2016; 1: N/D-N/D. Impact Factor:0 ViewExport to BibTeXExport to EndNote