Targeting of PED/PEA-15 molecular interaction with phospholipase D1 enhances insulin sensitivity in skeletal muscle cells (539 views)
Jbc Papers (ISSN: 0021-9258, 1083-351x, 0021-9258linking), 2008 Sep 1; 283(31): 21769-21778.
Export to BibTeX Export to EndNote
Paper type: Journal Article, Research Support, Non-U. S. Gov'T,
Impact factor: 5.52, 5-year impact factor: 5.575
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-52049085539&partnerID=40&md5=4f06778aafb5b4992d87f55c051a0032
Keywords: Glucose, Pea15 Protein, Mouse, Peptide, Phospholipase D, Phosphoprotein, Protein Kinase C Alpha, Phosphoprotein Enriched In Diabetes Phosphoprotein Enriched In Astrocytes 15, Unclassified Drug, Animal, Article, Biological Model, Chemistry, Cytology, Gene Deletion, Gene Vector, Genetics, Metabolism, Skeletal Muscle, Transgenic Mouse, Transport At The Cellular Level, Animal Cell, Binding Affinity, Carboxy Terminal Sequence, Controlled Study, Enzyme Activity, Enzyme Binding, Enzyme Linked Immunosorbent Assay, Gene Disruption, Gene Overexpression, Gene Targeting, Glucose Transport, In Vitro Study, Insulin Sensitivity, Molecular Interaction, Molecular Recognition, Muscle Cell, Non Insulin Dependent Diabetes Mellitus, Nonhuman, Priority Journal, Protein Binding, Protein Domain, Protein Protein Interaction, Protein Transport, Surface Plasmon Resonance, Transgenic Animal, Biological Transport, Genetic Vectors, Protein Kinase C-Alpha, Do-Mains, Glucose Uptakes, High Affinities, Insulin Regulations, Insulin Sensitivities, Intact Cells, Myocytes, Skeletal Muscle Cells, Type 2 Diabetes, Active Networks, Amines, Animal Cell Culture, Hormones, Molecular Structure, Plants (botany), Flow Interactions, Mus Musculus,
Affiliations:
*** IBB - CNR ***
Istituto di Biostrutture e Bioimmagini and Istituto di Endocrinologia e Oncologia Sperimentale Gaetano Salvatore, Consiglio Nazionale delle Ricerche, Naples, Italy.
Dipartimento di Scienze Biologiche, Università di Napoli Federico II, Naples 80131, Italy
Dipartimento di Biologia e Patologia Cellulare e Molecolare, Università di Napoli Federico II, Naples 80131, Italy
Tecnogen SpA, Località la Fagianeria, 81015 Piana di Monte Verna, Caserta, Italy
References:
Not available.
Jbc Papers (ISSN: 0021-9258, 1083-351x, 0021-9258linking), 2008 Sep 1; 283(31): 21769-21778.
Export to BibTeX Export to EndNote
Paper type: Journal Article, Research Support, Non-U. S. Gov'T,
Impact factor: 5.52, 5-year impact factor: 5.575
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-52049085539&partnerID=40&md5=4f06778aafb5b4992d87f55c051a0032
Keywords: Glucose, Pea15 Protein, Mouse, Peptide, Phospholipase D, Phosphoprotein, Protein Kinase C Alpha, Phosphoprotein Enriched In Diabetes Phosphoprotein Enriched In Astrocytes 15, Unclassified Drug, Animal, Article, Biological Model, Chemistry, Cytology, Gene Deletion, Gene Vector, Genetics, Metabolism, Skeletal Muscle, Transgenic Mouse, Transport At The Cellular Level, Animal Cell, Binding Affinity, Carboxy Terminal Sequence, Controlled Study, Enzyme Activity, Enzyme Binding, Enzyme Linked Immunosorbent Assay, Gene Disruption, Gene Overexpression, Gene Targeting, Glucose Transport, In Vitro Study, Insulin Sensitivity, Molecular Interaction, Molecular Recognition, Muscle Cell, Non Insulin Dependent Diabetes Mellitus, Nonhuman, Priority Journal, Protein Binding, Protein Domain, Protein Protein Interaction, Protein Transport, Surface Plasmon Resonance, Transgenic Animal, Biological Transport, Genetic Vectors, Protein Kinase C-Alpha, Do-Mains, Glucose Uptakes, High Affinities, Insulin Regulations, Insulin Sensitivities, Intact Cells, Myocytes, Skeletal Muscle Cells, Type 2 Diabetes, Active Networks, Amines, Animal Cell Culture, Hormones, Molecular Structure, Plants (botany), Flow Interactions, Mus Musculus,
Affiliations:
*** IBB - CNR ***
Istituto di Biostrutture e Bioimmagini and Istituto di Endocrinologia e Oncologia Sperimentale Gaetano Salvatore, Consiglio Nazionale delle Ricerche, Naples, Italy.
Dipartimento di Scienze Biologiche, Università di Napoli Federico II, Naples 80131, Italy
Dipartimento di Biologia e Patologia Cellulare e Molecolare, Università di Napoli Federico II, Naples 80131, Italy
Tecnogen SpA, Località la Fagianeria, 81015 Piana di Monte Verna, Caserta, Italy
References:
Not available.
Targeting of PED/PEA-15 molecular interaction with phospholipase D1 enhances insulin sensitivity in skeletal muscle cells
Phosphoprotein enriched in diabetes/phosphoprotein enriched in astrocytes (PED/PEA-15) is overexpressed in several tissues of individuals affected by type 2 diabetes. In intact cells and in transgenic animal models, PED/PEA-15 overexpression impairs insulin regulation of glucose transport, and this is mediated by its interaction with the C-terminal D4 domain of phospholipase D1 (PLD1) and the consequent increase of protein kinase C-alpha activity. Here we show that interfering with the interaction of PED/PEA-15 with PLD1 in L6 skeletal muscle cells overexpressing PED/PEA-15 (L6(PED/PEA-15)) restores insulin sensitivity. Surface plasmon resonance and ELISA-like assays show that PED/PEA-15 binds in vitro the D4 domain with high affinity (K(D) = 0.37 +/- 0.13 mum), and a PED/PEA-15 peptide, spanning residues 1-24, PED-(1-24), is able to compete with the PED/PEA-15-D4 recognition. When loaded into L6(PED/PEA-15) cells and in myocytes derived from PED/PEA-15-overexpressing transgenic mice, PED-(1-24) abrogates the PED/PEA-15-PLD1 interaction and reduces protein kinase C-alpha activity to levels similar to controls. Importantly, the peptide restores insulin-stimulated glucose uptake by approximately 70%. Similar results are obtained by expression of D4 in L6(PED/PEA-15). All these findings suggest that disruption of the PED/PEA-15-PLD1 molecular interaction enhances insulin sensitivity in skeletal muscle cells and indicate that PED/PEA-15 as an important target for type 2 diabetes.
Phosphoprotein enriched in diabetes/phosphoprotein enriched in astrocytes (PED/PEA-15) is overexpressed in several tissues of individuals affected by type 2 diabetes. In intact cells and in transgenic animal models, PED/PEA-15 overexpression impairs insulin regulation of glucose transport, and this is mediated by its interaction with the C-terminal D4 domain of phospholipase D1 (PLD1) and the consequent increase of protein kinase C-alpha activity. Here we show that interfering with the interaction of PED/PEA-15 with PLD1 in L6 skeletal muscle cells overexpressing PED/PEA-15 (L6(PED/PEA-15)) restores insulin sensitivity. Surface plasmon resonance and ELISA-like assays show that PED/PEA-15 binds in vitro the D4 domain with high affinity (K(D) = 0.37 +/- 0.13 mum), and a PED/PEA-15 peptide, spanning residues 1-24, PED-(1-24), is able to compete with the PED/PEA-15-D4 recognition. When loaded into L6(PED/PEA-15) cells and in myocytes derived from PED/PEA-15-overexpressing transgenic mice, PED-(1-24) abrogates the PED/PEA-15-PLD1 interaction and reduces protein kinase C-alpha activity to levels similar to controls. Importantly, the peptide restores insulin-stimulated glucose uptake by approximately 70%. Similar results are obtained by expression of D4 in L6(PED/PEA-15). All these findings suggest that disruption of the PED/PEA-15-PLD1 molecular interaction enhances insulin sensitivity in skeletal muscle cells and indicate that PED/PEA-15 as an important target for type 2 diabetes.
Targeting of PED/PEA-15 molecular interaction with phospholipase D1 enhances insulin sensitivity in skeletal muscle cells
No results. |
Targeting of PED/PEA-15 molecular interaction with phospholipase D1 enhances insulin sensitivity in skeletal muscle cells
Other filter: ([btitle,keywords] "Pea15 Protein" OR [b ...
Antonini D, Sirico A, Aberdam E, Ambrosio R, Campanile C, Fagoonee S, Altruda F, Aberdam D, Brissette JL, Missero C
* A composite enhancer regulates p63 gene expression in epidermal morphogenesis and in keratinocyte differentiation by multiple mechanisms (1066 views)
Nucleic Acids Res (ISSN: 0305-1048, 1362-4962), 2015 Jan; 43(2): 862-874.
Impact Factor: 9.202
View Export to BibTeX Export to EndNote
Farina B, Doti N, Pirone L, Malgieri G, Pedone E, Ruvo M, Fattorusso R
* Molecular basis of the PED/PEA15 interaction with the C-terminal fragment of phospholipase D1 revealed by NMR spectroscopy (649 views)
Bba-Gen Subjects (ISSN: 1570-9639, 0006-3002, 0925-4439), 2013 Sep; 1834(8): 1572-1580.
Impact Factor: 3.191
View Export to BibTeX Export to EndNote
Merolla F, Luise C, Muller MT, Pacelli R, Fusco A, Celetti A
* Loss of CCDC6, the First Identified RET Partner Gene, Affects pH2AX S139 Levels and Accelerates Mitotic Entry upon DNA Damage (538 views)
Plosone (ISSN: 1932-6203, 1932-6203electronic, 1932-6203linking), 2012 May 24; 7(5): N/D-N/D.
Impact Factor: 3.73
View Export to BibTeX Export to EndNote
Viparelli F, Doti N, Sandomenico A, Marasco D, Dathan NA, Miele C, Beguinot F, Monti SM, Ruvo M
* GSM48878: CD8 IL2-D2 (444 views)
Gene Expression Omnibus, 2011 Dec 2; 59(2): 302-308.
Impact Factor: 2.082
View Export to BibTeX Export to EndNote
Scognamiglio PL, Doti N, Grieco P, Pedone C, Ruvo M, Marasco D
* Discovery Of Small Peptide Antagonists Of Ped/Pea15-D4alpha Interaction From Simplified Combinatorial Libraries (596 views)
Chem Biol Drug Des (ISSN: 1747-0277, 1747-0285), 2011 May; 77(5): 319-327.
Impact Factor: 2.282
View Export to BibTeX Export to EndNote
Farina B, Pirone L, Russo L, Viparelli F, Doti N, Pedone C, Pedone E, Fattorusso R
* NMR backbone dynamics studies of human PED/PEA-15 outline protein functional sites (855 views)
Febs J (ISSN: 1742-4658, 1742-464x, 1742-4658electronic), 2010 Oct; 277(20): 4229-4240.
Impact Factor: 3.129
View Export to BibTeX Export to EndNote
Doti N, Cassese A, Marasco D, Paturzo F, Sabatella M, Viparelli F, Dathan N, Monti SM, Miele C, Formisano P, Beguinot F, Ruvo M
* Residues 762-801 of PLD1 mediate the interaction with PED/PEA15 (546 views)
Mol Biosyst (ISSN: 1742-206x), 2010 Oct; 6(10): 2039-2048.
Impact Factor: 3.825
View Export to BibTeX Export to EndNote
Sandomenico A, Monti SM, Sabatella M, De Capua A, Tornatore L, Doti N, Viparelli F, Dathan NA, Pedone C, Ruvo M, Marasco D
* Protein-protein interactions: a simple strategy to identify binding sites and peptide antagonists (759 views)
Chem Biol Drug Des (ISSN: 1747-0285, 1747-0277), 2009 May; 73(5): 483-493.
Impact Factor: 2.473
View Export to BibTeX Export to EndNote
Viparelli F, Doti N, Monti SM, Marasco D, Dathan N, Pedone C, Miele C, Formisano P, Beguinot F, Ruvo M
* Peptide antagonists of the PED-hPLD1 binding (434 views)
Adv Exp Med Biol (ISSN: 0065-2598, 0065-2598print, 0065-2598linking), 2009; 611: 445-446.
Impact Factor: 2.02
View Export to BibTeX Export to EndNote
Viparelli F, Doti N, Sandomenico A, Marasco D, Dathan NA, Miele C, Beguinot F, Monti SM, Ruvo M
* Expression and purification of the D4 region of PLD1 and characterization of its interaction with PED-PEA15 (551 views)
Protein Expr Purif (ISSN: 1046-5928), 2008 Jun; 59(2): 302-308.
Impact Factor: 1.621
View Export to BibTeX Export to EndNote
Fiory F, Alberobello AT, Miele C, Oriente F, Esposito I, Corbo V, Ruvo M, Tizzano B, Rasmussen TE, Gammeltoft S, Formisano P, Beguinot F
* Tyrosine phosphorylation of phosphoinositide-dependent kinase 1 by the insulin receptor is necessary for insulin metabolic signaling (529 views)
Mol Cell Biol (ISSN: 0270-7306, 1098-5549), 2005 Dec; 25(24): 10803-10814.
Impact Factor: 7.093
View Export to BibTeX Export to EndNote
Russo M, Palumbo R, Mupo A, Tosto M, Iacomino G, Scognamiglio A, Tedesco I, Galano G, Russo GL
* Flavonoid quercetin sensitizes a CD95-resistant cell line to apoptosis by activating protein kinase Cα (735 views)
Oncogene (ISSN: 0950-9232, 1476-5594), 2003; 22(21): 3330-3342.
Impact Factor: 6.495
View Export to BibTeX Export to EndNote
Scala S, Pacelli R, Iaffaioli RV, Normanno N, Pepe S, Frasci G, Genua G, Tsuruo T, Tagliaferri P, Bianco AR
Reversal of adriamycin resistance by recombinant α-interferon in multidrug-resistant human colon carcinoma LoVo-doxorubicin cells (683 views)
Cancer Res (ISSN: 0008-5472, 1538-7445, 1538-7445electronic), 1991 Sep 15; 51(18): 4898-4902.
Impact Factor: 8.426
View Export to BibTeX Export to EndNote
* A composite enhancer regulates p63 gene expression in epidermal morphogenesis and in keratinocyte differentiation by multiple mechanisms (1066 views)
Nucleic Acids Res (ISSN: 0305-1048, 1362-4962), 2015 Jan; 43(2): 862-874.
Impact Factor: 9.202
View Export to BibTeX Export to EndNote
Farina B, Doti N, Pirone L, Malgieri G, Pedone E, Ruvo M, Fattorusso R
* Molecular basis of the PED/PEA15 interaction with the C-terminal fragment of phospholipase D1 revealed by NMR spectroscopy (649 views)
Bba-Gen Subjects (ISSN: 1570-9639, 0006-3002, 0925-4439), 2013 Sep; 1834(8): 1572-1580.
Impact Factor: 3.191
View Export to BibTeX Export to EndNote
Merolla F, Luise C, Muller MT, Pacelli R, Fusco A, Celetti A
* Loss of CCDC6, the First Identified RET Partner Gene, Affects pH2AX S139 Levels and Accelerates Mitotic Entry upon DNA Damage (538 views)
Plosone (ISSN: 1932-6203, 1932-6203electronic, 1932-6203linking), 2012 May 24; 7(5): N/D-N/D.
Impact Factor: 3.73
View Export to BibTeX Export to EndNote
Viparelli F, Doti N, Sandomenico A, Marasco D, Dathan NA, Miele C, Beguinot F, Monti SM, Ruvo M
* GSM48878: CD8 IL2-D2 (444 views)
Gene Expression Omnibus, 2011 Dec 2; 59(2): 302-308.
Impact Factor: 2.082
View Export to BibTeX Export to EndNote
Scognamiglio PL, Doti N, Grieco P, Pedone C, Ruvo M, Marasco D
* Discovery Of Small Peptide Antagonists Of Ped/Pea15-D4alpha Interaction From Simplified Combinatorial Libraries (596 views)
Chem Biol Drug Des (ISSN: 1747-0277, 1747-0285), 2011 May; 77(5): 319-327.
Impact Factor: 2.282
View Export to BibTeX Export to EndNote
Farina B, Pirone L, Russo L, Viparelli F, Doti N, Pedone C, Pedone E, Fattorusso R
* NMR backbone dynamics studies of human PED/PEA-15 outline protein functional sites (855 views)
Febs J (ISSN: 1742-4658, 1742-464x, 1742-4658electronic), 2010 Oct; 277(20): 4229-4240.
Impact Factor: 3.129
View Export to BibTeX Export to EndNote
Doti N, Cassese A, Marasco D, Paturzo F, Sabatella M, Viparelli F, Dathan N, Monti SM, Miele C, Formisano P, Beguinot F, Ruvo M
* Residues 762-801 of PLD1 mediate the interaction with PED/PEA15 (546 views)
Mol Biosyst (ISSN: 1742-206x), 2010 Oct; 6(10): 2039-2048.
Impact Factor: 3.825
View Export to BibTeX Export to EndNote
Sandomenico A, Monti SM, Sabatella M, De Capua A, Tornatore L, Doti N, Viparelli F, Dathan NA, Pedone C, Ruvo M, Marasco D
* Protein-protein interactions: a simple strategy to identify binding sites and peptide antagonists (759 views)
Chem Biol Drug Des (ISSN: 1747-0285, 1747-0277), 2009 May; 73(5): 483-493.
Impact Factor: 2.473
View Export to BibTeX Export to EndNote
Viparelli F, Doti N, Monti SM, Marasco D, Dathan N, Pedone C, Miele C, Formisano P, Beguinot F, Ruvo M
* Peptide antagonists of the PED-hPLD1 binding (434 views)
Adv Exp Med Biol (ISSN: 0065-2598, 0065-2598print, 0065-2598linking), 2009; 611: 445-446.
Impact Factor: 2.02
View Export to BibTeX Export to EndNote
Viparelli F, Doti N, Sandomenico A, Marasco D, Dathan NA, Miele C, Beguinot F, Monti SM, Ruvo M
* Expression and purification of the D4 region of PLD1 and characterization of its interaction with PED-PEA15 (551 views)
Protein Expr Purif (ISSN: 1046-5928), 2008 Jun; 59(2): 302-308.
Impact Factor: 1.621
View Export to BibTeX Export to EndNote
Fiory F, Alberobello AT, Miele C, Oriente F, Esposito I, Corbo V, Ruvo M, Tizzano B, Rasmussen TE, Gammeltoft S, Formisano P, Beguinot F
* Tyrosine phosphorylation of phosphoinositide-dependent kinase 1 by the insulin receptor is necessary for insulin metabolic signaling (529 views)
Mol Cell Biol (ISSN: 0270-7306, 1098-5549), 2005 Dec; 25(24): 10803-10814.
Impact Factor: 7.093
View Export to BibTeX Export to EndNote
Russo M, Palumbo R, Mupo A, Tosto M, Iacomino G, Scognamiglio A, Tedesco I, Galano G, Russo GL
* Flavonoid quercetin sensitizes a CD95-resistant cell line to apoptosis by activating protein kinase Cα (735 views)
Oncogene (ISSN: 0950-9232, 1476-5594), 2003; 22(21): 3330-3342.
Impact Factor: 6.495
View Export to BibTeX Export to EndNote
Scala S, Pacelli R, Iaffaioli RV, Normanno N, Pepe S, Frasci G, Genua G, Tsuruo T, Tagliaferri P, Bianco AR
Reversal of adriamycin resistance by recombinant α-interferon in multidrug-resistant human colon carcinoma LoVo-doxorubicin cells (683 views)
Cancer Res (ISSN: 0008-5472, 1538-7445, 1538-7445electronic), 1991 Sep 15; 51(18): 4898-4902.
Impact Factor: 8.426
View Export to BibTeX Export to EndNote
13 Records (11 excluding Abstracts).
Total impact factor: 55.569 (42.637 excluding Abstracts).
Total 5 year impact factor: 55.271 (42.38 excluding Abstracts).
Total impact factor: 55.569 (42.637 excluding Abstracts).
Total 5 year impact factor: 55.271 (42.38 excluding Abstracts).
539 views. Last view on Monday 23 September 2024, 17:38:40
ibb.cnr.it
