Measurement of local Mr 97, 000 and 250, 000 protein antigen concentration in sections of human melanoma tumor using in vitro quantitative autoradiography
Measurement of local Mr 97, 000 and 250, 000 protein antigen concentration in sections of human melanoma tumor using in vitro quantitative autoradiography(580 views) Del Vecchio S, Reynolds JC, Blasberg RG, Neumann RD, Carrasquillo JA, Hellström I, Larson SM
Cancer Res (ISSN: 0008-5472, 1538-7445, 1538-7445electronic), 1988; 48(19): 5475-5481.
Affiliations: Nuclear Medicine Department, Clinical Center, NIH, Bethesda, MD 20892, United States
References: Not available.
Measurement of local Mr 97, 000 and 250, 000 protein antigen concentration in sections of human melanoma tumor using in vitro quantitative autoradiography
An assay method that uses 125I-labeled monoclonal antibody (MoAb) and in vitro quantitative autoradiography was developed to determine the local concentration of tumor-associated antigens in tissue sections. Human melanoma biopsy specimens were evaluated for the expression of the Mr 97,000 and 250,000 protein antigens using MoAb-96.5 and MoAb-9.2.27, respectively. Tissue sections were incubated in solutions of increasing concentration of 125I-labeled MoAb with or without an excess of unlabeled antibody. Quantitative autoradiography was performed on the sections and compared with 125I standards to determine tumor-bound radioactivity and calculate bound pmol of MoAb per g of tumor. The total binding, nonsaturable binding, and specific binding of 125I-labeled MoAb to tumor were then computed. Specific binding of MoAbs to tumor tissue was saturable in all antigen-positive tumors. The maximal concentration of specific binding of antibody to tissue (Bmax) represented the tissue antigen concentration. Estimates of the Ka of antigen/antibody binding were also made. The reliability of the measurements was confirmed by testing sections from mixtures of antigen-positive and antigen-negative cells.
Measurement of local Mr 97, 000 and 250, 000 protein antigen concentration in sections of human melanoma tumor using in vitro quantitative autoradiography
Measurement of local Mr 97, 000 and 250, 000 protein antigen concentration in sections of human melanoma tumor using in vitro quantitative autoradiography
Aloj L, Aurilio M, Rinaldi V, D'Ambrosio L, Tesauro D, Peitl PK, Maina T, Mansi R, Von Guggenberg E, Joosten L, Sosabowski JK, Breeman WA, De Blois E, Koelewijn S, Melis M, Waser B, Beetschen K, Reubi JC, De Jong M * The EEE project(545 views) Proc Int Cosm Ray Conf Icrc Universidad Nacional Autonoma De Mexico, 2007; 5(HEPART2): 977-980. Impact Factor:0 ViewExport to BibTeXExport to EndNote
Hesse B, Tagil K, Cuocolo A, Anagnostopoulos C, Bardies M, Bax J, Bengel F, Busemann Sokole E, Davies G, Dondi M, Edenbrandt L, Franken P, Kjaer A, Knuuti J, Lassmann M, Ljungberg M, Marcassa C, Marie PY, Mckiddie F, O'connor M, Prvuolovich E, Underwood R * 3. 0 T perfusion MR imaging(993 views) Rivista Di Neuroradiologia (ISSN: 1120-9976), 2004; 17(6): 807-812. Impact Factor:0.023 ViewExport to BibTeXExport to EndNote