Structure and biological activity of a conformational constrained apolipoprotein A-I-derived helical peptide targeting the protein haptoglobin (504 views)
Rsc Adv (ISSN: 2046-2069, 2046-2069electronic), 2014; 4(93): 51353-51361.
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Paper type: Journal Article,
Impact factor: 3.84, 5-year impact factor: 3.907
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84908242596&partnerID=40&md5=0f74360c110fa4dc4808b6ab9c6a814f
Keywords: Apolipoprotein A-I, Helical Peptide,
Affiliations:
*** IBB - CNR ***
Dipartimento di Biologia, Università di Napoli Federico II, Via Mezzocannone 8Napoli, Italy
Istituto di Biostrutture e Bioimmagini, CNR, Via Mezzocannone 16Napoli, Italy
Istituto per Il Sistema Produzione Animale in Ambiente Mediterraneo, CNR, via ArgineNapoli, Italy
Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Seconda Università di Napoli, via Vivaldi 43Caserta, Italy
References:
Not available.
Rsc Adv (ISSN: 2046-2069, 2046-2069electronic), 2014; 4(93): 51353-51361.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 3.84, 5-year impact factor: 3.907
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84908242596&partnerID=40&md5=0f74360c110fa4dc4808b6ab9c6a814f
Keywords: Apolipoprotein A-I, Helical Peptide,
Affiliations:
*** IBB - CNR ***
Dipartimento di Biologia, Università di Napoli Federico II, Via Mezzocannone 8Napoli, Italy
Istituto di Biostrutture e Bioimmagini, CNR, Via Mezzocannone 16Napoli, Italy
Istituto per Il Sistema Produzione Animale in Ambiente Mediterraneo, CNR, via ArgineNapoli, Italy
Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Seconda Università di Napoli, via Vivaldi 43Caserta, Italy
References:
Not available.
Structure and biological activity of a conformational constrained apolipoprotein A-I-derived helical peptide targeting the protein haptoglobin
The development of novel pharmacological treatments for atherosclerosis is an active research field in medicinal chemistry. It has been shown that the acute phase protein haptoglobin (Hpt) plays a role in modulating the reverse cholesterol transport binding to the HDL-major protein Apolipoprotein A-I, and it also plays a role in impairing the activity of the lecithin-cholesterol acyltransferase (LCAT). We reported that the peptide P2a in vitro and in vivo restores the LCAT activity in the presence of Hpt. Now, we have designed and characterized a conformational constrained P2a analogue, ApoAib, with the intention of improving Hpt binding and metabolic stability. Using non-proteogenic aminoisobutyric acid residues, we have obtained a well folded α-helical peptide with high proteolytic stability in serum. It binds to Hpt, impairs haptoglobin binding to HDL, and restores LCAT activity in the presence of haptoglobin. Furthermore, an interaction analysis using NMR revealed the peptide binding site involved in haptoglobin molecular recognition. In conclusion, ApoAib represents a promising candidate to improve reverse cholesterol transport for application in cardiovascular diseases. This journal is © the Partner Organisations 2014.
The development of novel pharmacological treatments for atherosclerosis is an active research field in medicinal chemistry. It has been shown that the acute phase protein haptoglobin (Hpt) plays a role in modulating the reverse cholesterol transport binding to the HDL-major protein Apolipoprotein A-I, and it also plays a role in impairing the activity of the lecithin-cholesterol acyltransferase (LCAT). We reported that the peptide P2a in vitro and in vivo restores the LCAT activity in the presence of Hpt. Now, we have designed and characterized a conformational constrained P2a analogue, ApoAib, with the intention of improving Hpt binding and metabolic stability. Using non-proteogenic aminoisobutyric acid residues, we have obtained a well folded α-helical peptide with high proteolytic stability in serum. It binds to Hpt, impairs haptoglobin binding to HDL, and restores LCAT activity in the presence of haptoglobin. Furthermore, an interaction analysis using NMR revealed the peptide binding site involved in haptoglobin molecular recognition. In conclusion, ApoAib represents a promising candidate to improve reverse cholesterol transport for application in cardiovascular diseases. This journal is © the Partner Organisations 2014.
Structure and biological activity of a conformational constrained apolipoprotein A-I-derived helical peptide targeting the protein haptoglobin
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Structure and biological activity of a conformational constrained apolipoprotein A-I-derived helical peptide targeting the protein haptoglobin
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Spagnuolo MS, Di Stasi R, De Rosa L, Maresca B, Cigliano L, D'Andrea LD
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Impact Factor: 2.406
View Export to BibTeX Export to EndNote
Bucci M, Cigliano L, Vellecco V, D'Andrea LD, Ziaco B, Rossi A, Sautebin L, Carlucci A, Abrescia P, Pedone C, Ianaro A, Cirino G
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Impact Factor: 3.891
View Export to BibTeX Export to EndNote
Monti DM(1), Di Gaetano S, Del Giudice R, Giangrande C, Amoresano A, Monti M, Arciello A, Piccoli R
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Impact Factor: 4.436
View Export to BibTeX Export to EndNote
Raimondi S, Guglielmi F, Giorgetti S, Di Gaetano S, Arciello A, Monti DM, Relini A, Nichino D, Doglia SM, Natalello A, Pucci P, Mangione P, Obici L, Merlini G, Stoppini M, Robustelli P, Tartaglia GG, Vendruscolo M, Dobson CM, Piccoli R, Bellotti V
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Impact Factor: 4.001
View Export to BibTeX Export to EndNote
Basile A, Del Gatto A, Diana D, Di Stasi R, Falco A, Festa M, Rosati A, Barbieri A, Franco R, Arra C, Pedone C, Fattorusso R, Turco MC, D'Andrea LD
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Impact Factor: 5.248
View Export to BibTeX Export to EndNote
Diana D, Ziaco B, Scarabelli G, Pedone C, Colombo G, D'Andrea LD, Fattorusso R
* Structural analysis of a helical peptide unfolding pathway (601 views)
Chemistry (ISSN: 1521-3765, 0947-6539, 1521-3765electronic), 2010 May 10; 16(18): 5400-5407.
Impact Factor: 5.476
View Export to BibTeX Export to EndNote
Cigliano L, Pugliese CR, Spagnuolo MS, Palumbo R, Abrescia P
* Haptoglobin binds the antiatherogenic protein apolipoprotein e - Impairment of apolipoprotein e stimulation of both lecithin: Cholesterol acyltransferase activity and cholesterol uptake by hepatocytes (523 views)
Febs J (ISSN: 1742-464x), 2009 Nov; 276(21): 6158-6171.
Impact Factor: 3.042
View Export to BibTeX Export to EndNote
Cigliano L, D'Andrea LD, Maresca B, Serino M, Carlucci A, Salvatore A, Spagnuolo MS, Scigliuolo G, Pedone C, Abrescia P
* Relevance of the amino acid conversions L144R (Zaragoza) and L159P (Zavalla) in the apolipoprotein A-I binding site for haptoglobin (434 views)
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Impact Factor: 3.035
View Export to BibTeX Export to EndNote
Cigliano L, Maresca B, Salvatore A, Nino M, Monfrecola G, Ayala F, Carlucci A, Pugliese RC, Pedone C, Abrescia P
* Haptoglobin from psoriatic patients exhibits decreased activity in binding haemoglobin and inhibiting lecithin-cholesterol acyltransferase activity (527 views)
Journal Of The European Academy Of Dermatology And Venereology (ISSN: 1468-3083, 0926-9959), 2008 Apr; 22(4): 417-425.
Impact Factor: 2.276
View Export to BibTeX Export to EndNote
Salvatore A, Cigliano L, Bucci EM, Corpillo D, Velasco S, Carlucci A, Pedone C, Abrescia P
* Haptoglobin binding to apolipoprotein A-I prevents damage from hydroxyl radicals on its stimulatory activity of the enzyme lecithin-cholesterol acyl-transferase (719 views)
Biochemistry (ISSN: 0006-2960, 1520-4995, 1520-4995electronic), 2007 Oct 2; 46(39): 11158-11168.
Impact Factor: 3.368
View Export to BibTeX Export to EndNote
D'Andrea LD, Iaccarino G, Fattorusso R, Sorriento D, Carannante C, Capasso D, Trimarco B, Pedone C
* Targeting angiogenesis: Structural characterization and biological properties of a de novo engineered VEGF mimicking peptide (726 views)
P Natl Acad Sci Usa (ISSN: 0027-8424, 1091-6490, 0027-8424print), 2005 Oct 4; 102(40): 14215-14220.
Impact Factor: 10.231
View Export to BibTeX Export to EndNote
Spagnuolo MS, Cigliano L, D'Andrea LD, Pedone C, Abrescia P
* Assignment of the binding site for haptoglobin on apolipoprotein A-I (661 views)
Jbc Papers (ISSN: 0021-9258, 1083-351x, 0021-9258linking), 2005 Jan 14; 280(2): 1193-1198.
Impact Factor: 5.854
View Export to BibTeX Export to EndNote
Impellizzeri G, Pappalardo G, Purrello R, Rizzarelli E, Santoro AM
Synthesis, spectroscopic characterisation, and metal ion interaction of a new α-helical peptide (631 views)
Chemistry (ISSN: 0947-6539, 1521-3765, 1521-3765electronic), 1998; 4(9): 1791-1798.
Impact Factor: 5.153
View Export to BibTeX Export to EndNote
18 Records (18 excluding Abstracts).
Total impact factor: 76.029 (76.029 excluding Abstracts).
Total 5 year impact factor: 74.925 (74.925 excluding Abstracts).
Total impact factor: 76.029 (76.029 excluding Abstracts).
Total 5 year impact factor: 74.925 (74.925 excluding Abstracts).
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