Protein P2 from Haemophilus influenzae: The role of its surface exposed loops on the mitogen-activated protein kinase cascade(539 views) Galdiero S, Capasso D, Vitiello M, D'Isanto M, Pedone C, Galdiero M
Infection And Immunity (ISSN: 0019-9567), 2003 May; 71(5): 2798-2809.
Keywords: Bacterial Protein, Mitogen Activated Protein Kinase, Outer Membrane Protein F, Porin, Raf Protein, Synthetic Peptide, Unclassified Drug, Amino Acid Sequence, Article, Bacterial Membrane, Enzyme Activation, Enzyme Mechanism, Haemophilus Influenzae, Haemophilus Influenzae Type B, Human, Human Cell, Klebsiella Pneumoniae, Nonhuman, Nucleotide Sequence, Priority Journal, Protein Analysis, Protein Domain, Protein Function, Protein Interaction, Protein Phoe, Protein Phosphorylation, Protein Tertiary Structure, Signal Transduction, Map Kinase Signaling System, Models, Molecular, Molecular Sequence Data, Protein Structure, Secondary, Sequence Alignment, Sequence Homology, U937 Cells,
Affiliations: *** IBB - CNR ***
Dipartimento di Chimica Biologica, Univ. Degli Studi Napoli Federico II, Ist. di Biostrutture e Bioimmagini, 80134 Naples, Italy
Dipartimento di Patologia Generale, Facolta di Medicina e Chirurgia, Seconda Università di Napoli, 80138 Naples, Italy
Dipto. di Medicina Sperimentale, Facolta di Medicina e Chirurgia, Seconda Università di Napoli, Via De Crecchio 7, 80138 Naples, Italy
References: Not available.
Protein P2 from Haemophilus influenzae: The role of its surface exposed loops on the mitogen-activated protein kinase cascade
The outer membrane of gram-negative bacteria contains several proteins, and some of these proteins, the porins, have numerous biological functions in the interaction with the host; porins are involved in the activation of signal transduction pathways and, in particular, in the activation of the Raf/MEK1-MEK2/ mitogen-activated protein kinase (MAPK) cascade. The P2 porin is the most abundant outer membrane protein of Haemophilus influenzae type b. A three-dimensional structural model for P2 was constructed based on the crystal structures of Klebsiella pneumoniae OmpK36 and Escherichia coli PhoE and OmpF. The protein was readily assembled into the beta-barrel fold characteristic of porins, despite the low sequence identity with the template proteins. The model provides information on the structural features of P2 and insights relevant for prediction of domains corresponding to surface-exposed loops, which could be involved in the activation of signal transduction pathways. To identify the role of surface-exposed loops, a set of synthetic peptides were synthesized according to the proposed model and were assayed for MEK1-MEK2/MAPK pathway activation. Our results show that synthetic peptides corresponding to surface loops of protein P2 are able to activate the MEK1-MEK2/MAPK pathways like the entire protein, while peptides modeled on internal 13 strands are unable to induce significant phosphorylation of the MEK1-MEK2/MAPK pathways. In particular, the peptides corresponding to loops L5 (Lys206 to Gly219), L613 (Ser239 to Lys253), and L7 (Thr280 to Lys287) activate, as the whole protein, essentially JNK and p38.
Protein P2 from Haemophilus influenzae: The role of its surface exposed loops on the mitogen-activated protein kinase cascade
Vitiello M, Finamore E, Falanga A, Raieta K, Cantisani M, Galdiero F, Pedone C, Galdiero M, Galdiero S * Fusion in Coq(600 views) Lecture Notes In Computer Science (ISSN: 0302-9743, 0302-974335404636319783540463634, 0302-974335402975459783540297543), 2001; 2178LNCS: 583-596. Impact Factor:0.415 ViewExport to BibTeXExport to EndNote