Rgdechi-Hcit: Alpha V Beta 3 Selective Pro-Apoptotic Peptide As Potential Carrier For Drug Delivery Into Melanoma Metastatic Cells(671 views) Capasso D, De Paola I, Liguoro A, Del Gatto A, Di Gaetano S, Guarnieri D, Saviano M, Zaccaro L
Keywords: Antineoplastic Agent, Caveolin, Clathrin, Drug Carrier, Polypeptide, Rgdechi Hcit Peptide, Unclassified Drug, Vitronectin Receptor, Adenocarcinoma Cell Line, Antineoplastic Activity, Antiproliferative Activity, Article, Binding Affinity, Binding Site, Cell Adhesion, Cell Assay, Cell Proliferation, Confocal Microscopy, Controlled Study, Drug Cytotoxicity, Drug Delivery System, Drug Effect, Drug Mechanism, Endocytosis, Flow Cytometry, Human, Human Cell, Melanoma Cell Line, Molecular Interaction, Protein Expression, Wm266 Cell Line,
Affiliations: *** IBB - CNR ***
Department of Pharmacy, University of Naples Federico IINaples, Italy
Institute of Biostructures and Bioimaging -CNRNaples, Italy
Diagnostic and Molecular Pharmaceutics -ScarlNaples, Italy
Center for Advanced Biomaterials for Health Care at CRIB, Italian Institute of TechnologyNaples, Italy
Institute of Crystallography-CNRBari, Italy
References: Not available.
Rgdechi-Hcit: Alpha V Beta 3 Selective Pro-Apoptotic Peptide As Potential Carrier For Drug Delivery Into Melanoma Metastatic Cells
alpha v beta 3 integrin is an important tumor marker widely expressed on the surface of cancer cells. Recently, we reported some biological features of RGDechi-hCit, an alpha v beta 3 selective peptide antagonist. In the present work, we mainly investigated the pro-apoptotic activity of the molecule and its ability to penetrate the membrane of WM266 cells, human malignant melanoma cells expressing high levels of alpha v beta 3 integrin. For the first time we demonstrated the pro-apoptotic effect and the ability of RGDechi-hCit to enter into cell overexpressing alpha v beta 3 integrin mainly by clathrin- and caveolin-mediated endocytosis. Furthermore, we deepened and confirmed the selectivity, anti-adhesion, and anti-proliferative features of the peptide. Altogether these experiments give insight into the biological behavior of RGDechi-hCit and have important implications for the employment of the peptide as a new selective carrier to deliver drugs into the cell and as a therapeutic and diagnostic tool for metastatic melanoma. Moreover, since the peptide shows a pro-apoptotic effect, a great perspective could be the development of a new class of selective systems containing RGDechi-hCit and pro-apoptotic molecules or other therapeutic agents to attain a synergic action
Rgdechi-Hcit: Alpha V Beta 3 Selective Pro-Apoptotic Peptide As Potential Carrier For Drug Delivery Into Melanoma Metastatic Cells
Petraglia F, Singh AA, Carafa V, Nebbioso A, Conte M, Scisciola L, Valente S, Baldi A, Mandoli A, Petrizzi VB, Ingenito C, De Falco S, Cicatiello V, Apicella I, Janssen-megens EM, Kim B, Yi G, Logie C, Heath S, Ruvo M, Wierenga ATJ, Flicek P, Yaspo ML, Della Valle V, Bernard O, Tomassi S, Novellino E, Feoli A, Sbardella G, Gut I, Vellenga E, Stunnenberg HG, Mai A, Martens JHA, Altucci L * Combined HAT/EZH2 modulation leads to cancer-selective cell death(450 views) Oncotarget (ISSN: 1949-2553electronic, 1949-2553linking), 2018 May 22; 9(39): 25630-25646. Impact Factor:5.008 ViewExport to BibTeXExport to EndNote
Kállay C, Dávid A, Timári S, Nagy EM, Sanna D, Garribba E, Micera G, De Bona P, Pappalardo G, Rizzarelli E, Sóvágó I * Copper(II) complexes of rat amylin fragments(554 views) Dalton T (ISSN: 1477-9234, 1477-9226, 1477-9234electronic), 2011 Oct 14; 40(38): 9711-9721. Impact Factor:3.838 ViewExport to BibTeXExport to EndNote