Structure and function of RNase AS, a polyadenylate-specific exoribonuclease affecting mycobacterial virulence in vivo (562 views)
Structure (ISSN: 0969-2126, 1878-4186, 0969-2126linking), 2014 May 6; 22(5): 719-730.
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Paper type: Journal Article, Research Support, Non-U. S. Gov'T,
Impact factor: 5.618, 5-year impact factor: 5.895
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84899892117&partnerID=40&md5=8f8402fcd2023c51024441736ea21ae2
Keywords: Adenine, Adenosine Phosphate, Exoribonuclease, Polyadenylic Acid, Uridine Phosphate, Article, Bacterial Virulence, Crystal Structure, Embryo, Enzyme Specificity, Enzyme Structure, Enzyme Substrate, Escherichia Coli, Hydrogen Bond, In Vivo Study, Maturation, Mycobacterium, Nonhuman, Priority Journal, Protein Family, Protein Function, Bacteria (microorganisms), Mycobacterium Tuberculosis,
Affiliations:
*** IBB - CNR ***
Institute of Biostructures and Bioimaging, National Research Council, Via Mezzocannone 16, I-80134 Naples, Italy
Department of Medical Microbiology and Infection Control, VU University Medical Center, Van der Boechorststraat 7, Amsterdam, 1081 BT, Netherlands
References:
Not available.
Structure (ISSN: 0969-2126, 1878-4186, 0969-2126linking), 2014 May 6; 22(5): 719-730.
Export to BibTeX Export to EndNote
Paper type: Journal Article, Research Support, Non-U. S. Gov'T,
Impact factor: 5.618, 5-year impact factor: 5.895
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84899892117&partnerID=40&md5=8f8402fcd2023c51024441736ea21ae2
Keywords: Adenine, Adenosine Phosphate, Exoribonuclease, Polyadenylic Acid, Uridine Phosphate, Article, Bacterial Virulence, Crystal Structure, Embryo, Enzyme Specificity, Enzyme Structure, Enzyme Substrate, Escherichia Coli, Hydrogen Bond, In Vivo Study, Maturation, Mycobacterium, Nonhuman, Priority Journal, Protein Family, Protein Function, Bacteria (microorganisms), Mycobacterium Tuberculosis,
Affiliations:
*** IBB - CNR ***
Institute of Biostructures and Bioimaging, National Research Council, Via Mezzocannone 16, I-80134 Naples, Italy
Department of Medical Microbiology and Infection Control, VU University Medical Center, Van der Boechorststraat 7, Amsterdam, 1081 BT, Netherlands
References:
Not available.
Structure and function of RNase AS, a polyadenylate-specific exoribonuclease affecting mycobacterial virulence in vivo
The cell-envelope of Mycobacterium tuberculosis plays a key role in bacterial virulence and antibiotic resistance. Little is known about the molecular mechanisms of regulation of cell-envelope formation. Here, we elucidate functional and structural properties of RNase AS, which modulates M. tuberculosis cell-envelope properties and strongly impacts bacterial virulence in vivo. The structure of RNase AS reveals a resemblance to RNase T from Escherichia coli, an RNase of the DEDD family involved in RNA maturation. We show that RNase AS acts as a 3′-5′-exoribonuclease that specifically hydrolyzes adenylate-containing RNA sequences. Also, crystal structures of complexes with AMP and UMP reveal the structural basis for the observed enzyme specificity. Notably, RNase AS shows a mechanism of substrate recruitment, based on the recognition of the hydrogen bond donor NH2 group of adenine. Our work opens a field for the design of drugs able to reduce bacterial virulence in vivo. © 2014 Elsevier Ltd.
The cell-envelope of Mycobacterium tuberculosis plays a key role in bacterial virulence and antibiotic resistance. Little is known about the molecular mechanisms of regulation of cell-envelope formation. Here, we elucidate functional and structural properties of RNase AS, which modulates M. tuberculosis cell-envelope properties and strongly impacts bacterial virulence in vivo. The structure of RNase AS reveals a resemblance to RNase T from Escherichia coli, an RNase of the DEDD family involved in RNA maturation. We show that RNase AS acts as a 3′-5′-exoribonuclease that specifically hydrolyzes adenylate-containing RNA sequences. Also, crystal structures of complexes with AMP and UMP reveal the structural basis for the observed enzyme specificity. Notably, RNase AS shows a mechanism of substrate recruitment, based on the recognition of the hydrogen bond donor NH2 group of adenine. Our work opens a field for the design of drugs able to reduce bacterial virulence in vivo. © 2014 Elsevier Ltd.
Structure and function of RNase AS, a polyadenylate-specific exoribonuclease affecting mycobacterial virulence in vivo
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Structure and function of RNase AS, a polyadenylate-specific exoribonuclease affecting mycobacterial virulence in vivo
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Improta R, Barone V
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Esposito C, Pethoukov MV, Svergun DI, Ruggiero A, Pedone C, Pedone E, Berisio R
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Chambery A, Pisante M, Di Maro A, Di Zazzo E, Ruvo M, Costantini S, Colonna G, Parente A
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* Unveiling CD59-Antibody Interactions to Design Paratope-Mimicking Peptides for Complement Modulation (131 views)
Int J Mol Sc (ISSN: 1422-0067linking, 1422-0067electronic), 2023 May 10; 24(10): 500-519.
Impact Factor: 6.21
View Export to BibTeX Export to EndNote
Romano M, Ruggiero A, Squeglia F, Maga G, Berisio R
* A Structural View of SARS-CoV-2 RNA Replication Machinery: RNA Synthesis, Proofreading and Final Capping (781 views)
Cells (ISSN: 2073-4409linking, 2073-4409electronic), 2020 May 20; 9(5): 1297-1297.
Impact Factor: 6.6
View Export to BibTeX Export to EndNote
Calvanese L, Squeglia F, Romano M, D'Auria G, Falcigno L, Berisio R
* Structural and dynamic studies provide insights into specificity and allosteric regulation of Ribonuclease AS, a key enzyme in mycobacterial virulence (333 views) (PDF 25 views)
J Biomol Struct Dyn (ISSN: 0739-1102linking, 1538-0254electronic), 2020; N/D: 1-19.
Impact Factor: 3.11
View Export to BibTeX Export to EndNote
Roviello GN
* Novel insights into nucleoamino acids: biomolecular recognition and aggregation studies of a thymine-conjugated L-phenyl alanine (498 views)
Amino Acids (ISSN: 1438-2199electronic, 0939-4451linking), 2018 May 15; DOI10.1007/s00726-01: 933-941.
Impact Factor: 3.173
View Export to BibTeX Export to EndNote
Musumeci D, Roviello V, Roviello GN
* DNA- and RNA-binding ability of oligoDapT, a nucleobase-decorated peptide, for biomedical applications (2372 views)
Int J Nanomed (ISSN: 1176-9114linking, 1178-2013electronic), 2018 May 1; 2018:13: 2613-2629.
Impact Factor: 4.3
View Export to BibTeX Export to EndNote
Romano M, Squeglia F, Berisio R
* Structure and Function of RNase AS, a Novel Virulence Factor from Mycobacterium Tuberculosis (587 views)
Curr Med Chem (ISSN: 0929-8673, 1875-533x, 1875-533xelectronic), 2015 Apr 17; 22(14): 1745-1756.
Impact Factor: 3.455
View Export to BibTeX Export to EndNote
Ruggiero A, De Simone P, Smaldone G, Squeglia F, Berisio R
* Bacterial cell division regulation by Ser/Thr kinases: A structural perspective (688 views)
Curr Protein Pept Sci (ISSN: 1389-2037, 1389-2037linking), 2012 Dec; 13(8): 756-766.
Impact Factor: 2.326
View Export to BibTeX Export to EndNote
Kaprelyants AS, Mukamolova GV, Ruggiero A, Makarov VA, Demina GR, Shleeva MO, Potapov VD, Shramko PA
* Resuscitation-promoting Factors (Rpf): In Search of Inhibitors (659 views)
Protein Pept Lett (ISSN: 0929-8665, 1875-5305), 2012 Oct; 19(10): 1026-1034.
Impact Factor: 1.994
View Export to BibTeX Export to EndNote
Improta R, Barone V
* Interplay between "neutral" and "charge-transfer" excimers rules the excited state decay in adenine-rich polynucleotides (754 views)
Angew Chem Int Ed (ISSN: 1433-7851, 1521-3773electronic, 1433-7851linking), 2011 Dec 9; 50(50): 12016-12019.
Impact Factor: 13.455
View Export to BibTeX Export to EndNote
Esposito C, Pethoukov MV, Svergun DI, Ruggiero A, Pedone C, Pedone E, Berisio R
* Evidence for an elongated dimeric structure of heparin-binding hemagglutinin from Mycobacterium tuberculosis (552 views)
J Bacteriol Journal Of Bacteriology (ISSN: 0021-9193, 1098-5530), 2008 Jul; 190(13): 4749-4753.
Impact Factor: 3.636
View Export to BibTeX Export to EndNote
Chambery A, Pisante M, Di Maro A, Di Zazzo E, Ruvo M, Costantini S, Colonna G, Parente A
* Invariant Ser211 is involved in the catalysis of PD-L4, type I RIP from Phytolacca dioica leaves (407 views)
Proteins (ISSN: 0887-3585, 1097-0134, 1097-0134electronic), 2007 Apr 1; 67(1): 209-218.
Impact Factor: 3.354
View Export to BibTeX Export to EndNote
De Falco S, Ruvoletto MG, Verdoliva A, Ruvo M, Raucci A, Marino M, Senatore S, Cassani G, Alberti A, Pontisso P, Fassina G
* Cloning and Expression of a Novel Hepatitis B Virus-binding Protein from HepG2 Cells (780 views)
Jbc Papers (ISSN: 0021-9258, 1083-351x, 0021-9258linking), 2001 Sep 28; 276(39): 36613-36623.
Impact Factor: 7.258
View Export to BibTeX Export to EndNote
12 Records (12 excluding Abstracts).
Total impact factor: 58.871 (58.871 excluding Abstracts).
Total 5 year impact factor: 57.447 (57.447 excluding Abstracts).
Total impact factor: 58.871 (58.871 excluding Abstracts).
Total 5 year impact factor: 57.447 (57.447 excluding Abstracts).
562 views. Last view on Sunday 13 October 2024, 9:35:51
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