SIRT1 gene expression upon genotoxic damage is regulated by APE1 through nCaRE-promoter elements (683 views)
Mol Biol Cell (ISSN: 1059-1524), 2014 Feb 15; 25(4): 532-547.
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Paper type: Journal Article,
Impact factor: 4.466, 5-year impact factor: 4.806
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84894235904&partnerID=40&md5=5d402e12c00ac3c057c0e38da15ee902
Keywords: Apex1 Protein, Human, Cell Nucleus Antigen, Dna (apurinic Or Apyrimidinic Site) Lyase, Dna Binding Protein, Dna Glycosyltransferase, Hydrogen Peroxide, Ku Antigen, Mesylic Acid Methyl Ester, Oxoguanine Glycosylase 1, Protein Binding, Rna Polymerase Ii, Sirt1 Protein, Sirtuin 1, Small Interfering Rna, Antagonists And Inhibitors, Binding Site, Biology, Dna Damage, Dna Responsive Element, Drug Effects, Gene Expression Regulation, Genetic Transcription, Hela Cell Line, Human Genome, Metabolism, Molecular Genetics, Nucleotide Sequence, Signal Transduction, Base Sequence, Computational Biology, Dna Glycosylases, Dna-(apurinic Or Apyrimidinic Site) Lyase, Dna-Binding Proteins, Methyl Methanesulfonate, Molecular Sequence Annotation, Molecular Sequence Data, Response Elements,
Affiliations:
*** IBB - CNR ***
Department of Biomedical Sciences and Technologies, University of Udine, 33100 Udine, Italy
Proteomics and Mass Spectrometry Laboratory, ISPAAM, National Research Council, 80147 Naples, Italy
Department of Pharmacy, University of Naples Federico II, 80134 Naples, Italy
Institute of Biostructures and Bioimaging, National Research Council, 80134 Naples, Italy
References:
Not available.
Mol Biol Cell (ISSN: 1059-1524), 2014 Feb 15; 25(4): 532-547.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 4.466, 5-year impact factor: 4.806
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84894235904&partnerID=40&md5=5d402e12c00ac3c057c0e38da15ee902
Keywords: Apex1 Protein, Human, Cell Nucleus Antigen, Dna (apurinic Or Apyrimidinic Site) Lyase, Dna Binding Protein, Dna Glycosyltransferase, Hydrogen Peroxide, Ku Antigen, Mesylic Acid Methyl Ester, Oxoguanine Glycosylase 1, Protein Binding, Rna Polymerase Ii, Sirt1 Protein, Sirtuin 1, Small Interfering Rna, Antagonists And Inhibitors, Binding Site, Biology, Dna Damage, Dna Responsive Element, Drug Effects, Gene Expression Regulation, Genetic Transcription, Hela Cell Line, Human Genome, Metabolism, Molecular Genetics, Nucleotide Sequence, Signal Transduction, Base Sequence, Computational Biology, Dna Glycosylases, Dna-(apurinic Or Apyrimidinic Site) Lyase, Dna-Binding Proteins, Methyl Methanesulfonate, Molecular Sequence Annotation, Molecular Sequence Data, Response Elements,
Affiliations:
*** IBB - CNR ***
Department of Biomedical Sciences and Technologies, University of Udine, 33100 Udine, Italy
Proteomics and Mass Spectrometry Laboratory, ISPAAM, National Research Council, 80147 Naples, Italy
Department of Pharmacy, University of Naples Federico II, 80134 Naples, Italy
Institute of Biostructures and Bioimaging, National Research Council, 80134 Naples, Italy
References:
Not available.
SIRT1 gene expression upon genotoxic damage is regulated by APE1 through nCaRE-promoter elements
Apurinic/apyrimidinic endonuclease 1 (APE1) is a multifunctional protein contributing to genome stability via repair of DNA lesions via the base excision repair pathway. It also plays a role in gene expression regulation and RNA metabolism. Another, poorly characterized function is its ability to bind to negative calcium responsive elements (nCaRE) of some gene promoters. The presence of many functional nCaRE sequences regulating gene transcription can be envisioned, given their conservation within ALU repeats. To look for functional nCaRE sequences within the human genome, we performed bioinformatic analyses and identified 57 genes potentially regulated by APE1. We focused on sirtuin-1 (SIRT1) deacetylase due to its involvement in cell stress, including senescence, apoptosis, and tumorigenesis, and its role in the deacetylation of APE1 after genotoxic stress. The human SIRT1 promoter presents two nCaRE elements stably bound by APE1 through its N-terminus. We demonstrate that APE1 is part of a multiprotein complex including hOGG1, Ku70, and RNA Pol II, which is recruited on SIRT1 promoter to regulate SIRT1 gene functions during early response to oxidative stress. These findings provide new insights into the role of nCaRE sequences in the transcriptional regulation of mammalian genes.
Apurinic/apyrimidinic endonuclease 1 (APE1) is a multifunctional protein contributing to genome stability via repair of DNA lesions via the base excision repair pathway. It also plays a role in gene expression regulation and RNA metabolism. Another, poorly characterized function is its ability to bind to negative calcium responsive elements (nCaRE) of some gene promoters. The presence of many functional nCaRE sequences regulating gene transcription can be envisioned, given their conservation within ALU repeats. To look for functional nCaRE sequences within the human genome, we performed bioinformatic analyses and identified 57 genes potentially regulated by APE1. We focused on sirtuin-1 (SIRT1) deacetylase due to its involvement in cell stress, including senescence, apoptosis, and tumorigenesis, and its role in the deacetylation of APE1 after genotoxic stress. The human SIRT1 promoter presents two nCaRE elements stably bound by APE1 through its N-terminus. We demonstrate that APE1 is part of a multiprotein complex including hOGG1, Ku70, and RNA Pol II, which is recruited on SIRT1 promoter to regulate SIRT1 gene functions during early response to oxidative stress. These findings provide new insights into the role of nCaRE sequences in the transcriptional regulation of mammalian genes.
SIRT1 gene expression upon genotoxic damage is regulated by APE1 through nCaRE-promoter elements
| ▼ Structural characterization of proteins and enzymes isolated from organisms living under unusual conditions |
SIRT1 gene expression upon genotoxic damage is regulated by APE1 through nCaRE-promoter elements
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Impact Factor: 4.779
View Export to BibTeX Export to EndNote
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Impact Factor: 3.73
View Export to BibTeX Export to EndNote
Fantini D, Vascotto C, Marasco D, D'Ambrosio C, Romanello M, Vitagliano L, Pedone C, Poletto M, Cesaratto L, Quadrifoglio F, Scaloni A, Radicella JP, Tell G
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Impact Factor: 7.836
View Export to BibTeX Export to EndNote
Malgieri G, Russo L, Esposito S, Baglivo I, Zaccaro L, Pedone EM, Di Blasio B, Isernia C, Pedone PV, Fattorusso R
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Impact Factor: 9.598
View Export to BibTeX Export to EndNote
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Impact Factor: 3.354
View Export to BibTeX Export to EndNote
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* A novel type of zinc finger DNA binding domain in the Agrobacterium tumefaciens transcriptional regulator Ros (1082 views)
Biochemistry (ISSN: 0006-2960, 1520-4995, 1520-4995electronic), 2006 Sep 29; 45(34): 10394-10405.
Impact Factor: 3.633
View Export to BibTeX Export to EndNote
Saviano M, Isernia C, Bassarello C, Di Lello P, Galdiero S, Mierke DF, Benedetti E, Pedone C
* Conformational analysis by NMR and distance geometry techniques of a peptide mimetic of the third helix of the Antennapedia homeodomain (573 views)
J Pept Res (ISSN: 1397-002x), 2005 Feb; 65(2): 200-208.
Impact Factor: 1.741
View Export to BibTeX Export to EndNote
Rivieccio V, Mannini R, Concilio L, D'Auria S, Pedone C, Grimaldi G
* Expression, purification and partial characterization of the Krüppel-associated box (KRAB) from the human ZNF2 protein (435 views)
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Impact Factor: 0.84
View Export to BibTeX Export to EndNote
Dathan N, Zaccaro L, Esposito S, Isernia C, Omichinski JG, Riccio A, Pedone C, Di Blasio B, Fattorusso R, Pedone PV
* The Arabidopsis SUPERMAN protein is able to specifically bind DNA through its single Cys(2)-His(2) zinc finger motif (1150 views)
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Impact Factor: 7.051
View Export to BibTeX Export to EndNote
Saviano M, Romanelli A, Bucci E, Pedone C, Mischiati C, Bianchi N, Feriotto G, Borgatti M, Gambari R
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Impact Factor: 1.826
View Export to BibTeX Export to EndNote
10 Records (9 excluding Abstracts).
Total impact factor: 44.388 (40.658 excluding Abstracts).
Total 5 year impact factor: 47.195 (42.951 excluding Abstracts).
Total impact factor: 44.388 (40.658 excluding Abstracts).
Total 5 year impact factor: 47.195 (42.951 excluding Abstracts).
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