Zinc To Cadmium Replacement In The Prokaryotic Zinc-Finger Domain(667 views) Malgieri G, Palmieri M, Esposito S, Maione V, Russo L, Baglivo I, De Paola I, Milardi D, Diana D, Zaccaro L, Pedone PV, Fattorusso R, Isernia C
Metallomics (ISSN: 1756-591x, 1756-5901, 1756-591xelectronic), 2013 Dec 18; 6(1): 96-104.
Keywords: Carcinogenic Effects, Cellular Contexts, Dna Binding Activity, Dna Binding Domain, Metal Substitution, Structural Differences, Zinc Finger, Zinc Finger Domains, Cadmium, Proteins, Nitrogen, Zinc Finger Protein, Article, Heteronuclear Single Quantum Coherence, Priority Journal, Protein Dna Binding, Protein Synthesis, Zinc Finger Motif, Agrobacterium Tumefaciens, Amino Acid Sequence, Bacterial Proteins, Binding Sites, Competitive, Dna-Binding Proteins, Magnetic Resonance Spectroscopy, Metalloproteins, Models, Molecular, Molecular Sequence Data, Mutation, Oligonucleotides, Protein Binding, Protein Conformation, Agrobacterium Tumefaciens Genetics Metabolism, Bacterial Proteins Chemistry Genetics Metabolism, Binding Sites Genetics, Cadmium Chemistry, Dna-Binding Proteins Chemistry Genetics Metabolism, Metalloproteins Chemistry Genetics Metabolism, Oligonucleotides Metabolism, Zinc Chemistry,
Affiliations: *** IBB - CNR ***
Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Via Vivaldi 43, 81100 Caserta, Italy
Institute of Biostructures and Bioimaging, CNR, Via Mezzocannone 16, 80134 Naples, Italy
Interuniversity Centre for Research on Bioactive Peptides (CIRPEB), University of Naples Federico II, Via Mezzocannone 16, 80134 Naples, Italy
References: Not available.
Zinc To Cadmium Replacement In The Prokaryotic Zinc-Finger Domain
Given the similar chemical properties of zinc and cadmium, zinc finger domains have been often proposed as mediators of the toxic and carcinogenic effects exerted by this xenobiotic metal. The effects of zinc replacement by cadmium in different eukaryotic zinc fingers have been reported. In the present work, to evaluate the effects of such substitution in the prokaryotic zinc finger, we report a detailed study of its functional and structural consequences on the Ros DNA binding domain (Ros87). We show that this protein, which bears important structural differences with respect to the eukaryotic domains, appears to structurally tolerate the zinc to cadmium substitution and the presence of cadmium does not affect the DNA binding activity of the protein. Moreover, we show for the first time how zinc to cadmium replacement can also take place in a cellular context. Our findings both complement and extend previous results obtained for different eukaryotic zinc fingers, suggesting that metal substitution in zinc fingers may be of relevance to the toxicity and/or carcinogenicity mechanisms of this metal. This journal is 2014 The Royal Society of Chemistry
Zinc To Cadmium Replacement In The Prokaryotic Zinc-Finger Domain
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Zinc To Cadmium Replacement In The Prokaryotic Zinc-Finger Domain