Integrin-dependent cell adhesion to neutrophil extracellular traps through engagement of fibronectin in neutrophil-like cells (392 views)
Plosone (ISSN: 1932-6203, 1932-6203electronic, 1932-6203linking), 2017 Feb; 12(2): 1-15.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 2.766, 5-year impact factor: 3.352
Url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85011654039&doi=10.1371%2fjournal.pone.0171362&partnerID=40&md5=1c1634e85a3b77ee6c7dc4edd4da9427
Keywords: Calcimycin, Deoxyribonuclease I, Fibronectin, Histone H3, Protein Antibody, Proteinase K, Very Late Activation Antigen 5, Vitronectin Receptor, Biological Marker, Integrin, Peroxidase, Protein Binding, Adsorption, Article, Binding Site, Cancer Cell, Cell Adhesion, Cell Stimulation, Confocal Microscopy, Controlled Study, Endothelium Cell, Extracellular Trap, Fluorescence Activated Cell Sorting, Hl-60 Cell Line, Human, Human Cell, Immunolocalization, Immunoprecipitation, K-562 Cell Line, Neutrophil, Protein Expression, Protein Interaction, Qualitative Analysis, Quantitative Analysis, Thrombocyte, Western Blotting, Metabolism, Physiology, Protein Transport, Biomarkers,
Affiliations:
*** IBB - CNR ***
Dipartimento di Medicina Clinica E Chirurgia, Università degli Studi di Napoli Federico II, Naples, Italy
Istituto di Biostrutture E Bioimmagini, Consiglio Nazionale Delle Ricerche, Naples, Italy
Dipartimento di Oncologia Sperimentale, IRCCS Istituto Nazionale Tumori Fondazione G. Pascale, Naples, Italy
Dipartimento di Scienze Biomediche Avanzate, Università degli Studi di Napoli Federico II, Naples, Italy
References:
Brinkmann, V., Reichard, U., Goosmann, C., Fauler, B., Uhlemann, Y., Weiss, D.S., Neutrophil extracellular traps kill bacteria (2004) Science, 303, pp. 1532-1535. , PMID: 1500178
Fuchs, T.A., Abed, U., Goosmann, C., Hurwitz, R., Schulze, I., Wahn, V., Novel cell death program leads to neutrophil extracellular traps (2007) J Cell Biol, 176, pp. 231-241. , PMID: 17210947
Fuchs, T.A., Brill, A., Duerschmied, D., Schatzberg, D., Monestier, M., Myers, D.D., Jr., Extracellular DNA traps promote thrombosis (2010) Proc Natl Acad Sci U S A, 107, pp. 15880-15885. , PMID: 20798043
Brill, A., Fuchs, T.A., Savchenko, A.S., Thomas, G.M., Martinod, K., De Meyer, S.F., Neutrophil extracellular traps promote deep vein thrombosis in mice (2012) J Thromb Haemost, 10, pp. 136-144. , PMID: 22044575
Cools-Lartigue, J., Spicer, J., McDonald, B., Gowing, S., Chow, S., Giannias, B., Neutrophil extracellular traps sequester circulating tumor cells and promote metastasis (2013) J Clin Invest, 123, pp. 3446-3458
Demers, M., Krause, D.S., Schatzberg, D., Martinod, K., Voorhees, J.R., Fuchs, T.A., Cancers predispose neutrophils to release extracellular DNA traps that contribute to cancer-associated thrombosis (2012) Proc Natl Acad Sci USA, 109, pp. 13076-13081. , PMID: 22826226
Tohme, S., Yazdani, H.O., Al-Khafaji, A.B., Chidi, A.P., Loughran, P., Mowen, K., Neutrophil extracellular traps promote the development and progression of liver metastases after surgical stress (2016) Cancer Res, 76, pp. 1367-1380. , PMID: 26759232
Avraamides, C.J., Garmy-Susini, B., Varner, J.A., Integrins in angiogenesis and lymphangiogenesis (2008) Nat Rev Cancer, 8, pp. 604-617. , PMID: 18497750
Hood, J.D., Cheresh, D.A., Role of integrins in cell invasion and migration (2002) Nat Rev Cancer, 2, pp. 91-100. , PMID: 12635172
Guo, W., Giancotti, F.G., Integrin signalling during tumour progression (2004) Nat Rev Mol Cell Biol, 5, pp. 816-826. , PMID: 15459662
Abram, C.L., Lowell, C.A., The ins and outs of leukocyte integrin signaling (2009) Annu Rev Immunol, 27, pp. 339-362. , PMID: 19302044
Luo, B.H., Carman, C.V., Springer, T.A., Structural basis of integrin regulation and signaling (2007) Annu Rev Immunol, 25, pp. 619-647. , PMID: 17201681
Humphries, J.D., Byron, A., Humphries, M.J., Integrin ligands at a glance (2006) J Cell Sci, 119, pp. 3901-3903. , PMID: 16988024
Brandau, S., Dumitru, C.A., Lang, S., Protumor and antitumor functions of neutrophil granulocytes (2013) Semin Immunopathol, 35, pp. 163-176. , PMID: 23007469
Desgrosellier, J.S., Cheresh, D.A., Integrins in cancer: Biological implications and therapeutic opportunities (2010) Nat Rev Cancer, 10, pp. 9-22. , PMID: 20029421
Rolli, M., Fransvea, E., Pilch, J., Saven, A., Felding-Habermann, B., Activated integrin alphavbeta3 cooperates with metalloproteinase MMP-9 in regulating migration of metastatic breast cancer cells (2003) Proc Natl Acad Sci USA, 100, pp. 9482-9487. , PMID: 12874388
Sloan, E.K., Pouliot, N., Stanley, K.L., Chia, J., Moseley, J.M., Hards, D.K., Tumor-specific expression of alphavbeta3 integrin promotes spontaneous metastasis of breast cancer to bone (2006) Breast Cancer Res, 8, p. R20. , PMID: 16608535
Carriero, M.V., Del Vecchio, S., Capozzoli, M., Franco, P., Fontana, L., Zannetti, A., Urokinase receptor interacts with alpha(v)beta5 vitronectin receptor, promoting urokinase-dependent cell migration in breast cancer (1999) Cancer Res, 59, pp. 5307-5314. , PMID: 10537314
Weber, M.R., Zuka, M., Lorger, M., Tschan, M., Torbett, B.E., Zijlstra, A., Activated tumor cell integrin alphavbeta3 cooperates with platelets to promote extravasation and metastasis from the blood stream (2016) Thromb Res, 140, pp. S27-S36
Chiaradonna, F., Fontana, L., Iavarone, C., Carriero, M.V., Scholz, G., Barone, M.V., Urokinase receptordependent and -independent p56/59(hck) activation state is a molecular switch between myelomonocytic cell motility and adherence (1999) EMBO J, 18, pp. 3013-3023. , PMID: 10357814
Wang, Y., Wysocka, J., Sayegh, J., Lee, Y.H., Perlin, J.R., Leonelli, L., Human PAD4 regulates histone arginine methylation levels via demethylimination (2004) Science, 306 (279-283), p. 15345777
Millius, A., Weiner, O.D., Chemotaxis in neutrophil-like HL-60 cells (2009) Methods Mol Biol, 571, pp. 167-177. , PMID: 19763966
Butler, B., Williams, M.P., Blystone, S.D., Ligand-dependent activation of integrin alpha vbeta 3 (2003) J Biol Chem, 278, pp. 5264-5270. , PMID: 12446696
Zannetti, A., Del Vecchio, S., Iommelli, F., Del Gatto, A., De Luca, S., Zaccaro, L., Imaging of alpha(v)beta (3) expression by a bifunctional chimeric RGD peptide not cross-reacting with alpha(v)beta(5) (2009) Clin Cancer Res, 15, pp. 5224-5233. , PMID: 19671851
Li, P., Li, M., Lindberg, M.R., Kennett, M.J., Xiong, N., Wang, Y., PAD4 is essential for antibacterial innate immunity mediated by neutrophil extracellular traps (2010) J Exp Med, 207, pp. 1853-1862. , PMID: 20733033
Douda, D.N., Khan, M.A., Grasemann, H., Palaniyar, N., SK3 channel and mitochondrial ROS mediate NADPH oxidase-independent NETosis induced by calcium influx (2015) Proc Natl Acad Sci U S A, 112, pp. 2817-2822. , PMID: 25730848
Najmeh, S., Cools-Lartigue, J., Giannias, B., Spicer, J., Ferri, L.E., Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling (2015) J Vis Exp
Barrientos, L., Marin-Esteban, V., De Chaisemartin, L., Le-Moal, V.L., Sandre, C., Bianchini, E., An improved strategy to recover large fragments of functional human neutrophil extracellular traps (2013) Front Immunol, 4, p. 166. , PMID: 23805143
Blystone, S.D., Graham, I.L., Lindberg, F.P., Brown, E.J., Integrin alpha v beta 3 differentially regulates adhesive and phagocytic functions of the fibronectin receptor alpha 5 beta 1 (1994) J Cell Biol, 127, pp. 1129-1137. , PMID: 7525603
Blystone, S.D., Lindberg, F.P., LaFlamme, S.E., Brown, E.J., Integrin beta 3 cytoplasmic tail is necessary and sufficient for regulation of alpha 5 beta 1 phagocytosis by alpha v beta 3 and integrin-associated protein (1995) J Cell Biol, 130, pp. 745-754. , PMID: 7542659
Pande, H., Calaycay, J., Hawke, D., Ben-Avram, C.M., Shively, J.E., Primary structure of a glycosylated DNAbinding domain in human plasma fibronectin (1985) J Biol Chem, 260, pp. 2301-2306. , PMID: 3882691
Chiron, D., Bekeredjian-Ding, I., Pellat-Deceunynck, C., Bataille, R., Jego, G., Toll-like receptors: Lessons to learn from normal and malignant human B cells (2008) Blood, 112, pp. 2205-2213. , PMID: 18591383
Ojo, O.O., Ryu, M.H., Jha, A., Unruh, H., Halayko, A.J., High-mobility group box 1 promotes extracellular matrix synthesis and wound repair in human bronchial epithelial cells (2015) Am J Physiol Lung Cell Mol Physiol, 309, pp. L1354-L1366. , PMID: 26432865
Remijsen, Q., Vanden Berghe, T., Wirawan, E., Asselbergh, B., Parthoens, E., De Rycke, R., Neutrophil extracellular trap cell death requires both autophagy and superoxide generation (2011) Cell Res, 21, pp. 290-304. , PMID: 21060338
La Fleur, M., Beaulieu, A.D., Kreis, C., Poubelle, P., Fibronectin gene expression in polymorphonuclear leukocytes. Accumulation of mRNA in inflammatory cells (1987) J Biol Chem, 262, pp. 2111-2115. , PMID: 3818588
Hoffstein, S.T., Weissmann, G., Pearlstein, E., Fibonectin is a component of the surface coat of human neutrophils (1981) J Cell Sci, 50, pp. 315-327. , PMID: 7033251
Pommier, C.G., O'Shea, J., Chused, T., Takahashi, T., Ochoa, M., Nutman, T.B., Differentiation stimuli induce receptors for plasma fibronectin on the human myelomonocytic cell line HL-60 (1984) Blood, 64, pp. 858-866. , PMID: 6236860
Plosone (ISSN: 1932-6203, 1932-6203electronic, 1932-6203linking), 2017 Feb; 12(2): 1-15.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 2.766, 5-year impact factor: 3.352
Url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85011654039&doi=10.1371%2fjournal.pone.0171362&partnerID=40&md5=1c1634e85a3b77ee6c7dc4edd4da9427
Keywords: Calcimycin, Deoxyribonuclease I, Fibronectin, Histone H3, Protein Antibody, Proteinase K, Very Late Activation Antigen 5, Vitronectin Receptor, Biological Marker, Integrin, Peroxidase, Protein Binding, Adsorption, Article, Binding Site, Cancer Cell, Cell Adhesion, Cell Stimulation, Confocal Microscopy, Controlled Study, Endothelium Cell, Extracellular Trap, Fluorescence Activated Cell Sorting, Hl-60 Cell Line, Human, Human Cell, Immunolocalization, Immunoprecipitation, K-562 Cell Line, Neutrophil, Protein Expression, Protein Interaction, Qualitative Analysis, Quantitative Analysis, Thrombocyte, Western Blotting, Metabolism, Physiology, Protein Transport, Biomarkers,
Affiliations:
*** IBB - CNR ***
Dipartimento di Medicina Clinica E Chirurgia, Università degli Studi di Napoli Federico II, Naples, Italy
Istituto di Biostrutture E Bioimmagini, Consiglio Nazionale Delle Ricerche, Naples, Italy
Dipartimento di Oncologia Sperimentale, IRCCS Istituto Nazionale Tumori Fondazione G. Pascale, Naples, Italy
Dipartimento di Scienze Biomediche Avanzate, Università degli Studi di Napoli Federico II, Naples, Italy
References:
Brinkmann, V., Reichard, U., Goosmann, C., Fauler, B., Uhlemann, Y., Weiss, D.S., Neutrophil extracellular traps kill bacteria (2004) Science, 303, pp. 1532-1535. , PMID: 1500178
Fuchs, T.A., Abed, U., Goosmann, C., Hurwitz, R., Schulze, I., Wahn, V., Novel cell death program leads to neutrophil extracellular traps (2007) J Cell Biol, 176, pp. 231-241. , PMID: 17210947
Fuchs, T.A., Brill, A., Duerschmied, D., Schatzberg, D., Monestier, M., Myers, D.D., Jr., Extracellular DNA traps promote thrombosis (2010) Proc Natl Acad Sci U S A, 107, pp. 15880-15885. , PMID: 20798043
Brill, A., Fuchs, T.A., Savchenko, A.S., Thomas, G.M., Martinod, K., De Meyer, S.F., Neutrophil extracellular traps promote deep vein thrombosis in mice (2012) J Thromb Haemost, 10, pp. 136-144. , PMID: 22044575
Cools-Lartigue, J., Spicer, J., McDonald, B., Gowing, S., Chow, S., Giannias, B., Neutrophil extracellular traps sequester circulating tumor cells and promote metastasis (2013) J Clin Invest, 123, pp. 3446-3458
Demers, M., Krause, D.S., Schatzberg, D., Martinod, K., Voorhees, J.R., Fuchs, T.A., Cancers predispose neutrophils to release extracellular DNA traps that contribute to cancer-associated thrombosis (2012) Proc Natl Acad Sci USA, 109, pp. 13076-13081. , PMID: 22826226
Tohme, S., Yazdani, H.O., Al-Khafaji, A.B., Chidi, A.P., Loughran, P., Mowen, K., Neutrophil extracellular traps promote the development and progression of liver metastases after surgical stress (2016) Cancer Res, 76, pp. 1367-1380. , PMID: 26759232
Avraamides, C.J., Garmy-Susini, B., Varner, J.A., Integrins in angiogenesis and lymphangiogenesis (2008) Nat Rev Cancer, 8, pp. 604-617. , PMID: 18497750
Hood, J.D., Cheresh, D.A., Role of integrins in cell invasion and migration (2002) Nat Rev Cancer, 2, pp. 91-100. , PMID: 12635172
Guo, W., Giancotti, F.G., Integrin signalling during tumour progression (2004) Nat Rev Mol Cell Biol, 5, pp. 816-826. , PMID: 15459662
Abram, C.L., Lowell, C.A., The ins and outs of leukocyte integrin signaling (2009) Annu Rev Immunol, 27, pp. 339-362. , PMID: 19302044
Luo, B.H., Carman, C.V., Springer, T.A., Structural basis of integrin regulation and signaling (2007) Annu Rev Immunol, 25, pp. 619-647. , PMID: 17201681
Humphries, J.D., Byron, A., Humphries, M.J., Integrin ligands at a glance (2006) J Cell Sci, 119, pp. 3901-3903. , PMID: 16988024
Brandau, S., Dumitru, C.A., Lang, S., Protumor and antitumor functions of neutrophil granulocytes (2013) Semin Immunopathol, 35, pp. 163-176. , PMID: 23007469
Desgrosellier, J.S., Cheresh, D.A., Integrins in cancer: Biological implications and therapeutic opportunities (2010) Nat Rev Cancer, 10, pp. 9-22. , PMID: 20029421
Rolli, M., Fransvea, E., Pilch, J., Saven, A., Felding-Habermann, B., Activated integrin alphavbeta3 cooperates with metalloproteinase MMP-9 in regulating migration of metastatic breast cancer cells (2003) Proc Natl Acad Sci USA, 100, pp. 9482-9487. , PMID: 12874388
Sloan, E.K., Pouliot, N., Stanley, K.L., Chia, J., Moseley, J.M., Hards, D.K., Tumor-specific expression of alphavbeta3 integrin promotes spontaneous metastasis of breast cancer to bone (2006) Breast Cancer Res, 8, p. R20. , PMID: 16608535
Carriero, M.V., Del Vecchio, S., Capozzoli, M., Franco, P., Fontana, L., Zannetti, A., Urokinase receptor interacts with alpha(v)beta5 vitronectin receptor, promoting urokinase-dependent cell migration in breast cancer (1999) Cancer Res, 59, pp. 5307-5314. , PMID: 10537314
Weber, M.R., Zuka, M., Lorger, M., Tschan, M., Torbett, B.E., Zijlstra, A., Activated tumor cell integrin alphavbeta3 cooperates with platelets to promote extravasation and metastasis from the blood stream (2016) Thromb Res, 140, pp. S27-S36
Chiaradonna, F., Fontana, L., Iavarone, C., Carriero, M.V., Scholz, G., Barone, M.V., Urokinase receptordependent and -independent p56/59(hck) activation state is a molecular switch between myelomonocytic cell motility and adherence (1999) EMBO J, 18, pp. 3013-3023. , PMID: 10357814
Wang, Y., Wysocka, J., Sayegh, J., Lee, Y.H., Perlin, J.R., Leonelli, L., Human PAD4 regulates histone arginine methylation levels via demethylimination (2004) Science, 306 (279-283), p. 15345777
Millius, A., Weiner, O.D., Chemotaxis in neutrophil-like HL-60 cells (2009) Methods Mol Biol, 571, pp. 167-177. , PMID: 19763966
Butler, B., Williams, M.P., Blystone, S.D., Ligand-dependent activation of integrin alpha vbeta 3 (2003) J Biol Chem, 278, pp. 5264-5270. , PMID: 12446696
Zannetti, A., Del Vecchio, S., Iommelli, F., Del Gatto, A., De Luca, S., Zaccaro, L., Imaging of alpha(v)beta (3) expression by a bifunctional chimeric RGD peptide not cross-reacting with alpha(v)beta(5) (2009) Clin Cancer Res, 15, pp. 5224-5233. , PMID: 19671851
Li, P., Li, M., Lindberg, M.R., Kennett, M.J., Xiong, N., Wang, Y., PAD4 is essential for antibacterial innate immunity mediated by neutrophil extracellular traps (2010) J Exp Med, 207, pp. 1853-1862. , PMID: 20733033
Douda, D.N., Khan, M.A., Grasemann, H., Palaniyar, N., SK3 channel and mitochondrial ROS mediate NADPH oxidase-independent NETosis induced by calcium influx (2015) Proc Natl Acad Sci U S A, 112, pp. 2817-2822. , PMID: 25730848
Najmeh, S., Cools-Lartigue, J., Giannias, B., Spicer, J., Ferri, L.E., Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling (2015) J Vis Exp
Barrientos, L., Marin-Esteban, V., De Chaisemartin, L., Le-Moal, V.L., Sandre, C., Bianchini, E., An improved strategy to recover large fragments of functional human neutrophil extracellular traps (2013) Front Immunol, 4, p. 166. , PMID: 23805143
Blystone, S.D., Graham, I.L., Lindberg, F.P., Brown, E.J., Integrin alpha v beta 3 differentially regulates adhesive and phagocytic functions of the fibronectin receptor alpha 5 beta 1 (1994) J Cell Biol, 127, pp. 1129-1137. , PMID: 7525603
Blystone, S.D., Lindberg, F.P., LaFlamme, S.E., Brown, E.J., Integrin beta 3 cytoplasmic tail is necessary and sufficient for regulation of alpha 5 beta 1 phagocytosis by alpha v beta 3 and integrin-associated protein (1995) J Cell Biol, 130, pp. 745-754. , PMID: 7542659
Pande, H., Calaycay, J., Hawke, D., Ben-Avram, C.M., Shively, J.E., Primary structure of a glycosylated DNAbinding domain in human plasma fibronectin (1985) J Biol Chem, 260, pp. 2301-2306. , PMID: 3882691
Chiron, D., Bekeredjian-Ding, I., Pellat-Deceunynck, C., Bataille, R., Jego, G., Toll-like receptors: Lessons to learn from normal and malignant human B cells (2008) Blood, 112, pp. 2205-2213. , PMID: 18591383
Ojo, O.O., Ryu, M.H., Jha, A., Unruh, H., Halayko, A.J., High-mobility group box 1 promotes extracellular matrix synthesis and wound repair in human bronchial epithelial cells (2015) Am J Physiol Lung Cell Mol Physiol, 309, pp. L1354-L1366. , PMID: 26432865
Remijsen, Q., Vanden Berghe, T., Wirawan, E., Asselbergh, B., Parthoens, E., De Rycke, R., Neutrophil extracellular trap cell death requires both autophagy and superoxide generation (2011) Cell Res, 21, pp. 290-304. , PMID: 21060338
La Fleur, M., Beaulieu, A.D., Kreis, C., Poubelle, P., Fibronectin gene expression in polymorphonuclear leukocytes. Accumulation of mRNA in inflammatory cells (1987) J Biol Chem, 262, pp. 2111-2115. , PMID: 3818588
Hoffstein, S.T., Weissmann, G., Pearlstein, E., Fibonectin is a component of the surface coat of human neutrophils (1981) J Cell Sci, 50, pp. 315-327. , PMID: 7033251
Pommier, C.G., O'Shea, J., Chused, T., Takahashi, T., Ochoa, M., Nutman, T.B., Differentiation stimuli induce receptors for plasma fibronectin on the human myelomonocytic cell line HL-60 (1984) Blood, 64, pp. 858-866. , PMID: 6236860
Integrin-dependent cell adhesion to neutrophil extracellular traps through engagement of fibronectin in neutrophil-like cells
Neutrophil extracellular traps (NETs), originally recognized as a host defense mechanism, were reported to promote thrombosis and metastatic dissemination of cancer cells. Here we tested the role of integrins α5β1 and αvβ3 in the adhesion of cancer cells to NETs. Neutrophil-like cells stimulated with calcium ionophore (A23187) were used as a stable source of cell-free NETs-enriched suspensions. Using NETs as an adhesion substrate, two human K562 cell lines, differentially expressing α5β1 and αvβ3 integrins, were subjected to adhesion assays in the presence or absence of DNAse 1, blocking antibodies against α5β1 or αvβ3, alone or in combination with DNAse 1, and Proteinase K. As expected DNAse 1 treatment strongly inhibited adhesion of both cell lines to NETs. An equivalent significant reduction of cell adhesion to NETs was obtained after treatment of cells with blocking antibodies against α5β1 or αvβ3 indicating that both integrins were able to mediate cell adhesion to NETs. Furthermore, the combination of DNAse 1 and anti-integrin antibody treatment almost completely blocked cell adhesion. Western blot analysis and immunoprecipitation experiments showed a dose-dependent increase of fibronectin levels in samples from stimulated neutrophil-like cells and a direct or indirect interaction of fibronectin with histone H3. Finally, co-immunolocalization studies with confocal microscopy showed that fibronectin and citrullinated histone H3 co-localize inside the web-structure of NETs. In conclusion, our study showed that α5β1 and αvβ3 integrins mediate cell adhesion to NETs by binding to their common substrate fibronectin. Therefore, in addition to mechanical trapping and aspecific adsorption of different cell types driven by DNA/histone complexes, NETs may provide specific binding sites for integrin-mediated cell adhesion of neutrophils, platelets, endothelial and cancer cells thus promoting intimate interactions among these cells. © 2017 Monti et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Neutrophil extracellular traps (NETs), originally recognized as a host defense mechanism, were reported to promote thrombosis and metastatic dissemination of cancer cells. Here we tested the role of integrins α5β1 and αvβ3 in the adhesion of cancer cells to NETs. Neutrophil-like cells stimulated with calcium ionophore (A23187) were used as a stable source of cell-free NETs-enriched suspensions. Using NETs as an adhesion substrate, two human K562 cell lines, differentially expressing α5β1 and αvβ3 integrins, were subjected to adhesion assays in the presence or absence of DNAse 1, blocking antibodies against α5β1 or αvβ3, alone or in combination with DNAse 1, and Proteinase K. As expected DNAse 1 treatment strongly inhibited adhesion of both cell lines to NETs. An equivalent significant reduction of cell adhesion to NETs was obtained after treatment of cells with blocking antibodies against α5β1 or αvβ3 indicating that both integrins were able to mediate cell adhesion to NETs. Furthermore, the combination of DNAse 1 and anti-integrin antibody treatment almost completely blocked cell adhesion. Western blot analysis and immunoprecipitation experiments showed a dose-dependent increase of fibronectin levels in samples from stimulated neutrophil-like cells and a direct or indirect interaction of fibronectin with histone H3. Finally, co-immunolocalization studies with confocal microscopy showed that fibronectin and citrullinated histone H3 co-localize inside the web-structure of NETs. In conclusion, our study showed that α5β1 and αvβ3 integrins mediate cell adhesion to NETs by binding to their common substrate fibronectin. Therefore, in addition to mechanical trapping and aspecific adsorption of different cell types driven by DNA/histone complexes, NETs may provide specific binding sites for integrin-mediated cell adhesion of neutrophils, platelets, endothelial and cancer cells thus promoting intimate interactions among these cells. © 2017 Monti et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Integrin-dependent cell adhesion to neutrophil extracellular traps through engagement of fibronectin in neutrophil-like cells
| ▼ Axl and Gas6 targeting for therapeutic and diagnostic applications |
Integrin-dependent cell adhesion to neutrophil extracellular traps through engagement of fibronectin in neutrophil-like cells
Other filter: ([btitle,keywords] "Calcimycin" OR [btit ...
Mcnitt DH, Choi SJ, Allen JL, Hames RA, Weed SA, Van De Water L, Berisio R, Lukomski S
* Adaptation of the group A Streptococcus adhesin Scl1 to bind fibronectin type III repeats within wound-associated extracellular matrix: implications for cancer therapy (202 views)
Mol Microbiol (ISSN: 0950-382xlinking), 2019 May 30; N/D: N/D-N/D.
Impact Factor: 3.816
View Export to BibTeX Export to EndNote
Mcnitt DH, De Water LV, Marasco D, Berisio R, Lukomski S
* Streptococcal Collagen-like Protein 1 Binds Wound Fibronectin: Implications in Pathogen Targeting (236 views)
Curr Med Chem (ISSN: 1875-533xelectronic, 0929-8673linking), 2019; 26: 1933-1945.
Impact Factor: 3.9
View Export to BibTeX Export to EndNote
Monti M, De Rosa V, Iommelli F, Carriero MV, Terlizzi C, Camerlingo R, Belli S, Fonti R, Di Minno G, Del Vecchio S
* Neutrophil Extracellular Traps as an Adhesion Substrate for Different Tumor Cells Expressing RGD-Binding Integrins (268 views)
Int J Mol Sc (ISSN: 1422-0067linking, 1422-0067electronic, 1661-6596), 2018 Aug 9; 19(8): N/D-N/D.
Impact Factor: 3.687
View Export to BibTeX Export to EndNote
Mcnitt DH, Choi SJ, Keene DR, Van De Water L, Squeglia F, Berisio R, Lukomski S
* Surface-exposed loops and an acidic patch in the Scl1 protein of group A Streptococcus enable Scl1 binding to wound-associated fibronectin (281 views)
J Biol Chem, 2018; N/D: N/D-N/D.
Impact Factor: 4.12
View Export to BibTeX Export to EndNote
Lukomski S, Bachert B, Squeglia F, Berisio R
* Collagen-like proteins of pathogenic streptococci (241 views)
Mol Microbiol (ISSN: 0950-382x), 2017; 103: 919-930.
Impact Factor: 3.816
View Export to BibTeX Export to EndNote
Pizzanelli S, Forte C, Pinzino C, Magrì A, La Mendola D
* Copper(ii) complexes with peptides based on the second cell binding site of fibronectin: metal coordination and ligand exchange kinetics (354 views)
Phys Chem Chem Phys (ISSN: 1463-9076, 1463-3907, 1463-9084electronic), 2016 Feb 7; 18(5): 3982-3994.
Impact Factor: 4.123
View Export to BibTeX Export to EndNote
Kaprelyants AS, Mukamolova GV, Ruggiero A, Makarov VA, Demina GR, Shleeva MO, Potapov VD, Shramko PA
* Resuscitation-promoting Factors (Rpf): In Search of Inhibitors (503 views)
Protein Pept Lett (ISSN: 0929-8665, 1875-5305), 2012 Oct; 19(10): 1026-1034.
Impact Factor: 1.994
View Export to BibTeX Export to EndNote
Magrì A, D'Alessandro F, Di Stefano DA, Campagna T, Pappalardo G, Impellizzeri G, La Mendola D
* Copper(ii) Coordination Properties Of The Integrin Ligand Sequence Phsrn And Its New Beta-Cyclodextrin Conjugates (488 views)
Journal Of Inorganic Biochemistry, 2012 Sep; 113: 15-24.
Impact Factor: 3.444
View Export to BibTeX Export to EndNote
Satriano C, Fragalà ME, Forte G, Santoro AM, La Mendola D, Kasemo B
* Surface adsorption of fibronectin-derived peptide fragments: The influence of electrostatics and hydrophobicity for endothelial cells adhesion (307 views)
Soft Matter (ISSN: 1744-683x), 2012; 8(1): 53-56.
Impact Factor: 3.909
View Export to BibTeX Export to EndNote
Del Vecchio S, Zannetti A, Iommelli F, Lettieri A, Brunetti A, Salvatore M
* Molecular imaging of tumor microenvironment (504 views)
Q J Nucl Med Mol Im (ISSN: 1824-4785, 1824-4478, 1824-4785linking), 2010 Jun; 54(3): 243-258.
Impact Factor: 1.724
View Export to BibTeX Export to EndNote
Del Vecchio S, Zannetti A, Iommelli F, Lettieri A, Brunetti A, Salvatore M
* Molecular imaging of tumor microenvironment: Challenges and perspectives (597 views)
Q J Nucl Med Mol Im (ISSN: 1824-4785, 1824-4478, 1824-4785linking), 2010 Jun; 54(3): 249-258.
Impact Factor: 1.724
View Export to BibTeX Export to EndNote
Satriano C, Messina GM, Marino C, Aiello I, Conte E, La Mendola D, Distefano DA, D'Alessandro F, Pappalardo G, Impellizzeri G
* Surface immobilization of fibronectin-derived PHSRN peptide on functionalized polymer films - Effects on fibroblast spreading (542 views)
J Colloid Interface Sci (ISSN: 1095-7103electronic, 0021-9797linking), 2010 Jan 15; 341(2): 232-239.
Impact Factor: 3.068
View Export to BibTeX Export to EndNote
Gioia M, Monaco S, Van Den Steen PE, Sbardella D, Grasso G, Marini S, Overall CM, Opdenakker G, Coletta M
* The Collagen Binding Domain of Gelatinase A Modulates Degradation of Collagen IV by Gelatinase (460 views)
J Mol Biol (ISSN: 0022-2836, 1089-8638, 1089-8638electronic), 2009; 386(2): 419-434.
Impact Factor: 3.871
View Export to BibTeX Export to EndNote
Merolla F, Pentimalli F, Pacelli R, Vecchio G, Fusco A, Grieco M, Celetti A
* Involvement of H4(D10S170) protein in ATM-dependent response to DNA damage (622 views)
Oncogene (ISSN: 0950-9232, 1476-5594), 2007 Sep 13; 26(42): 6167-6175.
Impact Factor: 6.44
View Export to BibTeX Export to EndNote
* Adaptation of the group A Streptococcus adhesin Scl1 to bind fibronectin type III repeats within wound-associated extracellular matrix: implications for cancer therapy (202 views)
Mol Microbiol (ISSN: 0950-382xlinking), 2019 May 30; N/D: N/D-N/D.
Impact Factor: 3.816
View Export to BibTeX Export to EndNote
Mcnitt DH, De Water LV, Marasco D, Berisio R, Lukomski S
* Streptococcal Collagen-like Protein 1 Binds Wound Fibronectin: Implications in Pathogen Targeting (236 views)
Curr Med Chem (ISSN: 1875-533xelectronic, 0929-8673linking), 2019; 26: 1933-1945.
Impact Factor: 3.9
View Export to BibTeX Export to EndNote
Monti M, De Rosa V, Iommelli F, Carriero MV, Terlizzi C, Camerlingo R, Belli S, Fonti R, Di Minno G, Del Vecchio S
* Neutrophil Extracellular Traps as an Adhesion Substrate for Different Tumor Cells Expressing RGD-Binding Integrins (268 views)
Int J Mol Sc (ISSN: 1422-0067linking, 1422-0067electronic, 1661-6596), 2018 Aug 9; 19(8): N/D-N/D.
Impact Factor: 3.687
View Export to BibTeX Export to EndNote
Mcnitt DH, Choi SJ, Keene DR, Van De Water L, Squeglia F, Berisio R, Lukomski S
* Surface-exposed loops and an acidic patch in the Scl1 protein of group A Streptococcus enable Scl1 binding to wound-associated fibronectin (281 views)
J Biol Chem, 2018; N/D: N/D-N/D.
Impact Factor: 4.12
View Export to BibTeX Export to EndNote
Lukomski S, Bachert B, Squeglia F, Berisio R
* Collagen-like proteins of pathogenic streptococci (241 views)
Mol Microbiol (ISSN: 0950-382x), 2017; 103: 919-930.
Impact Factor: 3.816
View Export to BibTeX Export to EndNote
Pizzanelli S, Forte C, Pinzino C, Magrì A, La Mendola D
* Copper(ii) complexes with peptides based on the second cell binding site of fibronectin: metal coordination and ligand exchange kinetics (354 views)
Phys Chem Chem Phys (ISSN: 1463-9076, 1463-3907, 1463-9084electronic), 2016 Feb 7; 18(5): 3982-3994.
Impact Factor: 4.123
View Export to BibTeX Export to EndNote
Kaprelyants AS, Mukamolova GV, Ruggiero A, Makarov VA, Demina GR, Shleeva MO, Potapov VD, Shramko PA
* Resuscitation-promoting Factors (Rpf): In Search of Inhibitors (503 views)
Protein Pept Lett (ISSN: 0929-8665, 1875-5305), 2012 Oct; 19(10): 1026-1034.
Impact Factor: 1.994
View Export to BibTeX Export to EndNote
Magrì A, D'Alessandro F, Di Stefano DA, Campagna T, Pappalardo G, Impellizzeri G, La Mendola D
* Copper(ii) Coordination Properties Of The Integrin Ligand Sequence Phsrn And Its New Beta-Cyclodextrin Conjugates (488 views)
Journal Of Inorganic Biochemistry, 2012 Sep; 113: 15-24.
Impact Factor: 3.444
View Export to BibTeX Export to EndNote
Satriano C, Fragalà ME, Forte G, Santoro AM, La Mendola D, Kasemo B
* Surface adsorption of fibronectin-derived peptide fragments: The influence of electrostatics and hydrophobicity for endothelial cells adhesion (307 views)
Soft Matter (ISSN: 1744-683x), 2012; 8(1): 53-56.
Impact Factor: 3.909
View Export to BibTeX Export to EndNote
Del Vecchio S, Zannetti A, Iommelli F, Lettieri A, Brunetti A, Salvatore M
* Molecular imaging of tumor microenvironment (504 views)
Q J Nucl Med Mol Im (ISSN: 1824-4785, 1824-4478, 1824-4785linking), 2010 Jun; 54(3): 243-258.
Impact Factor: 1.724
View Export to BibTeX Export to EndNote
Del Vecchio S, Zannetti A, Iommelli F, Lettieri A, Brunetti A, Salvatore M
* Molecular imaging of tumor microenvironment: Challenges and perspectives (597 views)
Q J Nucl Med Mol Im (ISSN: 1824-4785, 1824-4478, 1824-4785linking), 2010 Jun; 54(3): 249-258.
Impact Factor: 1.724
View Export to BibTeX Export to EndNote
Satriano C, Messina GM, Marino C, Aiello I, Conte E, La Mendola D, Distefano DA, D'Alessandro F, Pappalardo G, Impellizzeri G
* Surface immobilization of fibronectin-derived PHSRN peptide on functionalized polymer films - Effects on fibroblast spreading (542 views)
J Colloid Interface Sci (ISSN: 1095-7103electronic, 0021-9797linking), 2010 Jan 15; 341(2): 232-239.
Impact Factor: 3.068
View Export to BibTeX Export to EndNote
Gioia M, Monaco S, Van Den Steen PE, Sbardella D, Grasso G, Marini S, Overall CM, Opdenakker G, Coletta M
* The Collagen Binding Domain of Gelatinase A Modulates Degradation of Collagen IV by Gelatinase (460 views)
J Mol Biol (ISSN: 0022-2836, 1089-8638, 1089-8638electronic), 2009; 386(2): 419-434.
Impact Factor: 3.871
View Export to BibTeX Export to EndNote
Merolla F, Pentimalli F, Pacelli R, Vecchio G, Fusco A, Grieco M, Celetti A
* Involvement of H4(D10S170) protein in ATM-dependent response to DNA damage (622 views)
Oncogene (ISSN: 0950-9232, 1476-5594), 2007 Sep 13; 26(42): 6167-6175.
Impact Factor: 6.44
View Export to BibTeX Export to EndNote
14 Records (14 excluding Abstracts).
Total impact factor: 49.636 (49.636 excluding Abstracts).
Total 5 year impact factor: 50.253 (50.253 excluding Abstracts).
Total impact factor: 49.636 (49.636 excluding Abstracts).
Total 5 year impact factor: 50.253 (50.253 excluding Abstracts).
392 views. Last view on Wednesday 03 May 2023, 16:37:06
ibb.cnr.it
