New G-quadruplex-forming oligodeoxynucleotides incorporating a bifunctional Double-Ended Linker (DEL): effects of DEL size and ODNs orientation on the topology, stability and molecularity of DEL-G-quadruplexes
New G-quadruplex-forming oligodeoxynucleotides incorporating a bifunctional Double-Ended Linker (DEL): effects of DEL size and ODNs orientation on the topology, stability and molecularity of DEL-G-quadruplexes(386 views) Marzano M, Falanga AP, D'Errico S, Pinto B, Roviello GN, Piccialli G, Oliviero G, Borbone N
Molecules (ISSN: 1420-3049linking, 1420-3049electronic), 2019 Feb 12; 24(3): 654-654.
Dipartimento di Farmacia, Universita degli Studi di Napoli Federico II, via Domenico Montesano 49, 80131 Napoli, Italy. maria.marzano@unina.it.
Dipartimento di Chimica, Universita degli Studi di Milano, via Camillo Golgi 19, 20133 Milano, Italy. brunella.pinto87@gmail.com.
Istituto di Biostrutture e Bioimmagini, CNR, Via Tommaso De Amicis 95, 80145 Napoli, Italy. giroviel@unina.it.
Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Universita degli Studi di Napoli Federico II, via Sergio Pansini 5, 80131 Napoli, Italy. golivier@unina.it.
References: Not available.
New G-quadruplex-forming oligodeoxynucleotides incorporating a bifunctional Double-Ended Linker (DEL): effects of DEL size and ODNs orientation on the topology, stability and molecularity of DEL-G-quadruplexes
G-quadruplexes (G4s) are unusual secondary structures of DNA occurring
in guanosine-rich oligodeoxynucleotide (ODN) strands that are
extensively studied for their relevance to the biological processes in
which they are involved. In this study, we report the synthesis of a new
kind of G4-forming molecule named double-ended-linker ODN (DEL-ODN), in
which two TG
4
T strands are attached to the two ends of
symmetric, non-nucleotide linkers. Four DEL-ODNs differing for the
incorporation of either a short or long linker and the directionality of
the TG
4
T strands were synthesized, and their ability to form
G4 structures and/or multimeric species was investigated by PAGE,
HPLC–size-exclusion chromatography (HPLC–SEC), circular dichroism (CD),
and NMR studies in comparison with the previously reported monomeric
tetra-ended-linker (TEL) analogues and with the corresponding
tetramolecular species (TG
4
T)
4
. The structural
characterization of DEL-ODNs confirmed the formation of stable,
bimolecular DEL-G4s for all DEL-ODNs, as well as of additional DEL-G4
multimers with higher molecular weights, thus suggesting a way towards
the obtainment of thermally stable DNA nanostructures based on
reticulated DEL-G4s
New G-quadruplex-forming oligodeoxynucleotides incorporating a bifunctional Double-Ended Linker (DEL): effects of DEL size and ODNs orientation on the topology, stability and molecularity of DEL-G-quadruplexes
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New G-quadruplex-forming oligodeoxynucleotides incorporating a bifunctional Double-Ended Linker (DEL): effects of DEL size and ODNs orientation on the topology, stability and molecularity of DEL-G-quadruplexes