Multifaceted antibodies development against synthetic alpha-dystroglycan mucin glycopeptide as promising tools for dystroglycanopathies diagnostic (230 views)
Glycoconj J (ISSN: 0282-0080linking), 2020 Feb; 37(1): 77-93.
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Paper type: Journal Article, Research Support, Non-U. S. Gov'T,
Impact factor: 2.197, 5-year impact factor: 0
Url: Not available.
Keywords: Antibodies, Glycopeptide, Mucins, Phage Display, Molecular Modelling, Alpha-Dystroglycan, α-Dystroglycan,
Affiliations:
*** IBB - CNR ***
Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Av. Cafe S/N, CEP, Ribeirao Preto, SP, 14040-903, Brazil.
Centro Universitario Barao de Maua, Rua Ramos de Azevedo 423, Jardim Paulista, CEP, Ribeirao Preto, 14090-180, SP, Brazil.
Instituto de Ciencias Biologicas, Universidade de Brasilia, Asa Norte, Brasilia, DF, CEP 70910-900, Brazil.
Istituto di Biostrutture e Bioimmagini, CNR, via Mezzocannone, 16, 80134, Naples, Italy.
References:
Not available.
Glycoconj J (ISSN: 0282-0080linking), 2020 Feb; 37(1): 77-93.
Export to BibTeX Export to EndNote
Paper type: Journal Article, Research Support, Non-U. S. Gov'T,
Impact factor: 2.197, 5-year impact factor: 0
Url: Not available.
Keywords: Antibodies, Glycopeptide, Mucins, Phage Display, Molecular Modelling, Alpha-Dystroglycan, α-Dystroglycan,
Affiliations:
*** IBB - CNR ***
Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Av. Cafe S/N, CEP, Ribeirao Preto, SP, 14040-903, Brazil.
Centro Universitario Barao de Maua, Rua Ramos de Azevedo 423, Jardim Paulista, CEP, Ribeirao Preto, 14090-180, SP, Brazil.
Instituto de Ciencias Biologicas, Universidade de Brasilia, Asa Norte, Brasilia, DF, CEP 70910-900, Brazil.
Istituto di Biostrutture e Bioimmagini, CNR, via Mezzocannone, 16, 80134, Naples, Italy.
References:
Not available.
Multifaceted antibodies development against synthetic alpha-dystroglycan mucin glycopeptide as promising tools for dystroglycanopathies diagnostic
Dystroglycanopathies are diseases characterized by progressive muscular degeneration and impairment of patient's quality of life. They are associated with altered glycosylation of the dystrophin-glycoprotein (DGC) complex components, such as alpha-dystroglycan (alpha-DG), fundamental in the structural and functional stability of the muscle fiber. The diagnosis of dystroglycanopathies is currently based on the observation of clinical manifestations, muscle biopsies and enzymatic measures, and the available monoclonal antibodies are not specific for the dystrophic hypoglycosylated muscle condition. Thus, modified alpha-DG mucins have been considered potential targets for the development of new diagnostic strategies toward these diseases. In this context, this work describes the synthesis of the hypoglycosylated alpha-DG mimetic glycopeptide NHAc-Gly-Pro-Thr-Val-Thr[alphaMan]-Ile-Arg-Gly-BSA (1) as a potential tool for the development of novel antibodies applicable to dystroglycanopathies diagnosis. Glycopeptide 1 was used for the development of polyclonal antibodies and recombinant monoclonal antibodies by Phage Display technology. Accordingly, polyclonal antibodies were reactive to glycopeptide 1, which enables the application of anti-glycopeptide 1 antibodies in immune reactive assays targeting hypoglycosylated alpha-DG. Regarding monoclonal antibodies, for the first time variable heavy (VH) and variable light (VL) immunoglobulin domains were selected by Phage Display, identified by NGS and described by in silico analysis. The best-characterized VH and VL domains were cloned, expressed in E. coli Shuffle T7 cells, and used to construct a single chain fragment variable that recognized the Glycopeptide 1 (GpalphaDG1 scFv). Molecular modelling of glycopeptide 1 and GpalphaDG1 scFv suggested that their interaction occurs through hydrogen bonds and hydrophobic contacts involving amino acids from scFv (I51, Y33, S229, Y235, and P233) and R8 and alpha-mannose from Glycopeptide 1.
Dystroglycanopathies are diseases characterized by progressive muscular degeneration and impairment of patient's quality of life. They are associated with altered glycosylation of the dystrophin-glycoprotein (DGC) complex components, such as alpha-dystroglycan (alpha-DG), fundamental in the structural and functional stability of the muscle fiber. The diagnosis of dystroglycanopathies is currently based on the observation of clinical manifestations, muscle biopsies and enzymatic measures, and the available monoclonal antibodies are not specific for the dystrophic hypoglycosylated muscle condition. Thus, modified alpha-DG mucins have been considered potential targets for the development of new diagnostic strategies toward these diseases. In this context, this work describes the synthesis of the hypoglycosylated alpha-DG mimetic glycopeptide NHAc-Gly-Pro-Thr-Val-Thr[alphaMan]-Ile-Arg-Gly-BSA (1) as a potential tool for the development of novel antibodies applicable to dystroglycanopathies diagnosis. Glycopeptide 1 was used for the development of polyclonal antibodies and recombinant monoclonal antibodies by Phage Display technology. Accordingly, polyclonal antibodies were reactive to glycopeptide 1, which enables the application of anti-glycopeptide 1 antibodies in immune reactive assays targeting hypoglycosylated alpha-DG. Regarding monoclonal antibodies, for the first time variable heavy (VH) and variable light (VL) immunoglobulin domains were selected by Phage Display, identified by NGS and described by in silico analysis. The best-characterized VH and VL domains were cloned, expressed in E. coli Shuffle T7 cells, and used to construct a single chain fragment variable that recognized the Glycopeptide 1 (GpalphaDG1 scFv). Molecular modelling of glycopeptide 1 and GpalphaDG1 scFv suggested that their interaction occurs through hydrogen bonds and hydrophobic contacts involving amino acids from scFv (I51, Y33, S229, Y235, and P233) and R8 and alpha-mannose from Glycopeptide 1.
Multifaceted antibodies development against synthetic alpha-dystroglycan mucin glycopeptide as promising tools for dystroglycanopathies diagnostic
Multifaceted antibodies development against synthetic alpha-dystroglycan mucin glycopeptide as promising tools for dystroglycanopathies diagnostic
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Fonti R, Levchenko A, Mehta BM, Zhang J, Tsuruo T, Larson SM
Measurement of P-glycoprotein expression in human neuroblastoma xenografts using in vitro quantitative autoradiography (378 views)
Nucl Med Biol (ISSN: 0969-8051, 0883-2897), 1999 Jan; 26(1): 35-41.
Impact Factor: 1.623
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Monti SM, Fogliano V, Randazzo G, Peluso G, Logrieco A, Ritieni A
Polyclonal antibodies against fusaproliferin (361 views)
Can J Microbiol (ISSN: 0008-4166), 1999 Jan; 45(1): 45-50.
Impact Factor: 1.072
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Fonti R, Cheung N-KV, Bridger GJ, Guo H-F, Abrams MJ, Larson SM
99mTc-monoclonal antibody radiolabeled via hydrazino nicotinamide derivative for imaging disialoganglioside G(D2)-positive tumors (549 views)
Nucl Med Biol (ISSN: 0969-8051, 0883-2897), 1999; 26(6): 681-686.
Impact Factor: 1.623
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Renda A, Iovino F, Capasso L, Ricciardelli L, Tammaro V, Acampa W
Radioimmunoguided surgery in colorectal cancer: A 6-year experience with four different technical solutions (401 views)
Semin Surg Oncol Wiley Liss Inc (ISSN: 8756-0437), 1998 Dec; 15(4): 226-230.
Impact Factor: 1.045
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Pace L, Catalano L, Pinto A, De Renzo A, Di Gennaro F, Califano C, Del Vecchio S, Rotoli B, Salvatore M
Different patterns of technetium-99m sestamibi uptake in multiple myeloma (481 views)
Eur J Nucl Med (ISSN: 0340-6997, 1619-7070, 1619-7089), 1998 Jul; 25(7): 714-720.
Impact Factor: 2.564
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Levchenko A, Mehta B, Spengler BA, Narkar A, Fonti R, Biedler JL, Tsuruo T, Larson SM
Measurement of P-glycoprotein expression in multidrug-resistant human neuroblastoma cell lines using self-competitive binding assay (368 views)
Anal Biochem (ISSN: 0003-2697, 1096-0309electronic, 0003-2697linking), 1996 May 1; 236(2): 338-343.
Impact Factor: 2.017
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Li PY, Del Vecchio S, Fonti R, Carriero MV, Potena MI, Botti G, Miotti S, Lastoria S, Menard S, Colnaghi MI, Salvatore M
Local concentration of folate binding protein GP38 in sections of human ovarian carcinoma by in vitro quantitative autoradiography (545 views)
J Nucl Med (ISSN: 0161-5505, 1535-5667, 1535-5667electronic), 1996 Apr; 37(4-6): 665-672.
Impact Factor: 3.491
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Carriero MV, Franco P, Del Vecchio S, Massa O, Botti G, D'Aiuto G, Stoppelli MP, Salvatore M
Tissue distribution of soluble and receptor-bound urokinase in human breast cancer using a panel of monoclonal antibodies (565 views)
Cancer Res (ISSN: 0008-5472, 1538-7445, 1538-7445electronic), 1994 Oct 15; 54(20): 5445-5454.
Impact Factor: 8.426
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Caraglia M, Tagliaferri P, Correale P, Genua G, Pinto A, Del Vecchio S, Esposito G, Bianco AR
CYTOSINE-ARABINOSIDE INCREASES THE BINDING OF I-125 LABELED EPIDERMAL GROWTH-FACTOR AND I-125 TRANSFERRIN AND ENHANCES THE IN-VITRO TARGETING OF HUMAN TUMOR-CELLS WITH ANTI-(GROWTH FACTOR-RECEPTOR) MAB (364 views)
Cancer Immunol Immunother (ISSN: 0340-7004, 0340-7004linking), 1993 Sep; 37(3): 150-156.
Impact Factor: 1.269
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Caraglia M, Tagliaferri P, Correale P, Genua G, Pinto A, Del Vecchio S, Esposito G, Bianco AR
Cytosine arabinoside increases the binding of 125I-labelled epidermal growth factor and 125I-transferrin and enhances the in vitro targeting of human tumour cells with anti-(growth factor receptor) mAb (341 views)
Cancer Immunol Immunother (ISSN: 0340-7004linking), 1993 Aug; 37 (3 ): 150-156.
Impact Factor: 4.293
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D'Aiuto G, Del Vecchio S, Mansi L, D'Aprile M, Botti G, Salvatore M
Malignant melanoma of the nipple: A case studied with radiolabeled monoclonal antibody (728 views)
Tumori (ISSN: 0300-8916, 0300-8916print, 0300-8916linking), 1991 Oct 31; 77(5): 449-451.
Impact Factor: 0.408
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Manna R, Salvatore M, Di Leo MA, Scuderi F, Greco AV, Ghirlanda G, Gambassi G
Relationship between urinary neopterin excretion and islet cell antibodies in type 1 (insulin-dependent) diabetes (596 views)
Diabetes Res (ISSN: 0265-5985), 1991 May; 17(1): 33-36.
Impact Factor: 0
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Camera L, Del Vecchio S, Petrillo A, Esposito G, Frasci G, Iaffaioli RV, Bianco AR, Salvatore M
Evaluation of therapeutic response using iodine-131-B72. 3 monoclonal antibody in patients with ovarian carcinoma (603 views)
Eur J Nucl Med (ISSN: 0340-6997, 1619-7070, 1619-7089), 1991 Apr; 18(4): 269-273.
Impact Factor: 2.692
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Del Vecchio S, Camera L, Petrillo A, Esposito G, Frasci G, Iaffaioli RV, Bianco AR, Salvatore M
Clinical studies with B72. 3 in ovarian cancer and probe-guided tumor localization (763 views)
Nucl Med Biol (ISSN: 0883-2897, 0969-8051), 1991; 18(1): 85-87.
Impact Factor: 1.042
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Del Vecchio S, Mansi L, Petrillo A, Camera L, Sofia M, Marra A, Carratú L, Salvatore M
Endobronchial administration of iodine-131 B72. 3 monoclonal antibody in patients with lung cancer (349 views)
Eur J Nucl Med (ISSN: 0340-6997, 1619-7070, 1619-7089), 1991; 18(2): 129-132.
Impact Factor: 2.692
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Papa V, Reese CC, Brunetti A, Vigneri R, Siiteri PK, Goldfine ID
Progestins increase insulin receptor content and insulin stimulation of growth in human breast carcinoma cells (593 views)
Cancer Res (ISSN: 0008-5472, 1538-7445, 1538-7445electronic), 1990 Dec 15; 50(24): 7858-7862.
Impact Factor: 8.426
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Valenza V, Maussier ML, D'Errico G, Lemmo GF, Frontera D, Ferrante A, Saletnich I, Valli A, Quarantelli M
Cancer of the pancreas and the periampullary region: The usefulness of tumor marker products with the monoclonal antibody technique (378 views)
J Nucl Med Allied Sci (ISSN: 0392-0208), 1990 Oct; 34(4SUPPL.): 131-134.
Impact Factor: 1.91
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Del Vecchio S, Reynolds JC
NONUNIFORM DISTRIBUTION OF RADIOLABELED MONOCLONAL-ANTIBODY IN TUMOR (337 views)
J Nucl Med (ISSN: 0161-5505, 1535-5667, 1535-5667electronic), 1990 Jul; 31(7): 1252-1252.
Impact Factor: 3.491
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Steis RG, Carrasquillo JA, Mccabe R, Bookman MA, Reynolds JC, Larson SM, Smith JW, Clark JW, Dailey V, Del Vecchio S
Toxicity, immunogenicity, and tumor radioimmunodetecting ability of two human monoclonal antibodies in patients with metastatic colorectal carcinoma (367 views)
J Clin Oncol (ISSN: 0732-183x, 0732-183xlinking), 1990 Mar; 8(3): 476-490.
Impact Factor: 7.878
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Trischitta V, Wong KY, Brunetti A, Scalisi R, Vigneri R, Goldfine ID
Endocytosis, recycling, and degradation of the insulin receptor. Studies with monoclonal antireceptor antibodies that do not activate receptor kinase (366 views)
Jbc Papers (ISSN: 0021-9258, 1083-351x, 0021-9258linking), 1989 Mar 25; 264(9): 5041-5046.
Impact Factor: 6.963
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d'Onofrio G, Menichella G, Zini G, Rumi C, Pierelli L, Quarantelli M, Puggioni PL, Mango G
Lymphocyte subset counting by immunoperoxidase using an automated routine hematologic analyzer (477 views)
Haematologica (ISSN: 0390-6078, 0006-4971, 0006-6497), 1989 Mar; 74(2): 137-142.
Impact Factor: 2.56
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Reynolds JC, Del Vecchio S, Sakahara H, Lora ME, Carrasquillo JA, Neumann RD, Larson SM
ANTI-MURINE ANTIBODY RESPONSE TO MOUSE MONOCLONAL ANTIBODIES CLINICAL FINDINGS AND IMPLICATIONS (922 views)
Nucl Med Biol (ISSN: 0969-8051, 0883-2897), 1989; 16(2): 121-125.
Impact Factor: 1.042
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Salvatore M, Lastoria S, Del Vecchio S, Mansi L
Detection of colon cancer with radiolabelled monoclonal antibodies (460 views)
Nucl Med Biol (ISSN: 0883-2897, 0969-8051), 1989; 16(2): 103-104.
Impact Factor: 1.042
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Del Vecchio S, Reynolds JC, Carrasquillo JA, Blasberg RG, Neumann RD, Lotze MT, Bryant GJ, Farkas RJ, Larson SM
Local distribution and concentration of intravenously injected 131I-9. 2. 27 monoclonal antibody in human malignant melanoma (426 views)
Cancer Res (ISSN: 0008-5472, 1538-7445, 1538-7445electronic), 1989; 49(10): 2783-2789.
Impact Factor: 8.426
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Manna R, Salvatore M, Scuderi F, Papa G, Marietti G, Greco AV, Ghirlanda G, Gambassi G
Negative correlation between ICA persistence and beta cell restoration after IDDM diagnosis (407 views)
Diabetes Res (ISSN: 0265-5985), 1988 Nov; 9(3): 101-103.
Impact Factor: 0
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Del Vecchio S, Reynolds JC, Blasberg RG, Neumann RD, Carrasquillo JA, Hellström I, Larson SM
Measurement of local Mr 97, 000 and 250, 000 protein antigen concentration in sections of human melanoma tumor using in vitro quantitative autoradiography (569 views)
Cancer Res (ISSN: 0008-5472, 1538-7445, 1538-7445electronic), 1988; 48(19): 5475-5481.
Impact Factor: 8.426
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* A Blood Test for the Diagnosis of Multiple Sclerosis (29 views)
Int J Mol Sci (ISSN: 1422-0067linking), 2024 Jan 30; 25 (3 ): N/D-N/D.
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Troisi R, Balasco N, Autiero I, Vitagliano L, Sica F
* Structural Insights into Protein-Aptamer Recognitions Emerged from Experimental and Computational Studies (7 views)
Int J Mol Sci (ISSN: 1422-0067linking), 2023 Nov 14; 24 (22 ): N/D-N/D.
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Musumeci D, Ullah S, Ikram A, Roviello GN
* Novel insights on nucleopeptide binding: a spectroscopic and in silico investigation on the interaction of a thymine-bearing tetrapeptide with a homoadenine DNA (205 views)
Journal Of Molecular Liquids, 2021 Dec; 117975: 14140-14148.
Impact Factor: 6.165
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Moreira M, Ruggiero A, Iaccarino E, Barra G, Sandomenico A, Ruvo M, Berisio R
* A structure-based approach for the development of a bicyclic peptide acting as a miniaturized anti-CD55 antibody (179 views) (PDF 33 views)
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Impact Factor: 6.95
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Sivaccumar JP, Leonardi A, Iaccarino E, Corvino G, Sanguigno L, Chambery A, Russo R, Valletta M, Latino D, Capasso D, Doti N, Ruvo M, Sandomenico A
* Development of a New Highly Selective Monoclonal Antibody against Preferentially Expressed Antigen in Melanoma (PRAME) and Identification of the Target Epitope by Bio-Layer Interferometry (80 views)
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Impact Factor: 4.556
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Sivaccumar J, Sandomenico A, Vitagliano L, Ruvo M
* Monoclonal Antibodies: A Prospective and Retrospective View (183 views)
Curr Med Chem (ISSN: 0929-8673linking, 1875-533xelectronic), 2021; 28(3): 435-471.
Impact Factor: 4.184
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Di Stasi R, De Rosa L, Diana D, Fattorusso R, D'Andrea LD
* Human Recombinant VEGFR2D4 Biochemical Characterization to Investigate Novel Anti-VEGFR2D4 Antibodies for Allosteric Targeting of VEGFR2 (353 views)
Mol Biotechnol (ISSN: 1073-6085linking, 1559-0305electronic), 2019 Apr 26; 61(7): 513-520.
Impact Factor: 2.275
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Foca G, Iaccarino E, Foca A, Sanguigno L, Untiveros G, Cuevas-nunez M, Strizzi L, Leonardi A, Ruvo M, Sandomenico A
* Development of conformational antibodies targeting Cripto-1 with neutralizing effects in vitro (650 views)
Biochimie (ISSN: 0300-9084linking, 1638-6183electronic), 2019 Mar; 158: 246-256.
Impact Factor: 3.787
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Supuran CT, Alterio V, Di Fiore A, D'Ambrosio K, Carta F, Monti SM, De Simone G
* Inhibition of carbonic anhydrase IX targets primary tumors, metastases, and cancer stem cells: Three for the price of one (744 views)
Med Res Rev (ISSN: 1098-1128electronic, 0198-6325linking), 2018 Sep; 38(6): 1799-1836.
Impact Factor: 8.29
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Calce E, Digilio G, Menchise V, Saviano M, De Luca S
* Chemoselective Glycosylation of Peptides through S-Alkylation Reaction (830 views) (PDF 189 views)
Chemistry (ISSN: 1521-3765electronic, 0947-6539linking), 2018 Apr 20; 24(23): 6231-6238.
Impact Factor: 5.476
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Calvanese L, Foca A, Sandomenico A, Foca G, Caporale A, Doti N, Iaccarino E, Leonardi A, D'Auria G, Ruvo M, Falcigno L
* Structural insights into the interaction of a monoclonal antibody and Nodal peptides by STD-NMR spectroscopy (581 views)
Bioorg Med Chem (ISSN: 0968-0896, 1464-3391, 0968-0896linking), 2017 Dec 15; 25(24): 6589-6596.
Impact Factor: 2.881
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Maglio O, Costanzo S, Cercola R, Zambrano G, Mauro M, Battaglia R, Ferrini G, Nastri F, Pavone V, Lombardi A
* A Quartz Crystal Microbalance Immunosensor for Stem Cell Selection and Extraction (1408 views) (PDF 169 views)
Sensors (ISSN: 1424-8220, 1424-3210, 1424-8220linking), 2017 Nov 28; 17(12): N/D-N/D.
Impact Factor: 2.475
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Leiria Campo V, Riul TB, Oliveira Bortot L, Martins-teixeira MB, Fiori Marchiori M, Iaccarino E, Ruvo M, Dias-baruffi M, Carvalho I
* A Synthetic MUC1 Glycopeptide Bearing betaGalNAc-Thr as a Tn Antigen Isomer Induces the Production of Antibodies against Tumor Cells (1645 views)
Chembiochem (ISSN: 1439-4227, 1439-7633, 1439-4227linking), 2017 Mar 16; 18(6): 527-538.
Impact Factor: 2.774
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Barone D, Balasco N, Autiero I, Vitagliano L
* The dynamic properties of the Hepatitis C Virus E2 envelope protein unraveled by molecular dynamics (7 views)
J Biomol Struct Dyn (ISSN: 0739-1102linking), 2017 Mar; 35 (4 ): 805-816.
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Sandomenico A, Focà A, Sanguigno L, Caporale A, Focà G, Pignalosa A, Corvino G, Caragnano A, Beltrami AP, Antoniali G, Tell G, Leonardi A, Ruvo M
* Monoclonal antibodies against pools of mono- and polyacetylated peptides selectively recognize acetylated lysines within the context of the original antigen (426 views) (PDF 232 views)
Mabs-Austin (ISSN: 1942-0862), 2016 Aug; 8: 1575-1589.
Impact Factor: 4.881
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Quero G, Consales M, Severino R, Vaiano P, Boniello A, Sandomenico A, Ruvo M, Borriello A, Diodato L, Zuppolini S, Giordano M, Nettore IC, Mazzarella C, Colao A, Macchia PE, Santorelli F, Cutolo A, Cusano A
* Long period fiber grating nano-optrode for cancer biomarker detection (393 views) (PDF 221 views)
Biosens Bioelectron (ISSN: 0956-5663, 1873-4235electronic), 2016 Feb 13; 80: 590-600.
Impact Factor: 7.78
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Sandomenico A, Leonardi A, Berisio R, Sanguigno L, Foca G, Foca A, Ruggiero A, Doti N, Muscariello L, Barone D, Farina C, Owsianka A, Vitagliano L, Patel AH, Ruvo M
* Generation and characterization of monoclonal antibodies against a cyclic variant of Hepatitis C Virus E2 epitope 412-422 (653 views) (PDF 215 views)
J Virol (ISSN: 0022-538x, 0022-538xlinking), 2016 Jan 27; 90(7): 3745-3759.
Impact Factor: 4.663
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Bogdanovich S, Kim Y, Mizutani T, Yasuma R, Tudisco L, Cicatiello V, Bastos-carvalho A, Kerur N, Hirano Y, Baffi JZ, Tarallo V, Li S, Yasuma T, Arpitha P, Fowler BJ, Wright CB, Apicella I, Greco A, Brunetti A, Ruvo M, Sandomenico A, Nozaki M, Ijima R, Kaneko H, Ogura Y, Terasaki H, Ambati BK, Leusen JH, Langdon WY, Clark MR, Armour KL, Bruhns P, Verbeek JS, Gelfand BD, De Falco S, Ambati J
* Human IgG1 antibodies suppress angiogenesis in a target-independent manner (652 views)
Signal Transduct Target Ther (ISSN: 2059-3635print), 2016; 1: N/D-N/D.
Impact Factor: 0
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Palladino P, Aura AM, Spoto G
* Surface plasmon resonance for the label-free detection of Alzheimer's β-amyloid peptide aggregation (589 views)
Anal Bioanal Chem (ISSN: 1618-2642), 2016; 408(3): 849-854.
Impact Factor: 3.431
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Foca A, Sanguigno L, Foca G, Strizzi L, Iannitti R, Palumbo R, Hendrix MJ, Leonardi A, Ruvo M, Sandomenico A
* New Anti-Nodal Monoclonal Antibodies Targeting the Nodal Pre-Helix Loop Involved in Cripto-1 Binding (802 views) (PDF 290 views)
Int J Mol Sc (ISSN: 1422-0067, 1661-6596, 1422-0067linking), 2015 Sep 7; 16(9): 21342-21362.
Impact Factor: 3.257
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Klauzinska M, Bertolette D, Tippireddy S, Strizzi L, Gray PC, Gonzales M, Duroux M, Ruvo M, Wechselberger C, Castro NP, Rangel MC, Focà A, Sandomenico A, Hendrix MJC, Salomon D, Cuttitta F
* Cripto-1: an extracellular protein – connecting the sequestered biological dots (471 views)
Connect Tissue Res (ISSN: 0300-8207), 2015; N/D: N/D-N/D.
Impact Factor: 1.411
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Comegna D, de Paola I, Saviano M, Del Gatto A, Zaccaro L
* Straightforward entry to s-glycosylated fmoc-amino acids and their application to solid phase synthesis of glycopeptides and glycopeptidomimetics (672 views)
Org Lett (ISSN: 1523-7060, 1523-7052, 1523-7052electronic), 2015; 17(3): 640-643.
Impact Factor: 6.732
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Lanzillo R, Liuzzi R, Vallefuoco L, Moccia M, Amato L, Vacca G, Vacchiano V, Portella G, Brescia Morra V
* JC virus antibody index in natalizumab-treated patients: correlations with John Cunningham virus DNA and C-reactive protein level (500 views)
Ther Clin Risk Manag (ISSN: 1178-203x), 2014 Oct 9; 10: 807-814.
Impact Factor: 1.469
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Gallicchio R, Mansueto G, Simeon V, Nardelli A, Guariglia R, Capacchione D, Soscia E, Pedicini P, Gattozzi D, Musto P, Storto G
* F-18 FDG PET/CT quantization parameters as predictors of outcome in patients with diffuse large B-cell lymphoma (738 views)
Eur J Haematol (ISSN: 0902-4441), 2014 May; 92(5): 382-389.
Impact Factor: 2.066
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Lanzillo R, Bonavita S, Quarantelli M, Vacca G, Lus G, Amato L, Carotenuto A, Tedeschi G, Orefice G, Brescia Morra V
* Natalizumab is effective in multiple sclerosis patients switching from other disease modifying therapies in clinical practice (412 views)
Neurol Sci (ISSN: 1590-1874, 1590-0187, 1590-3478), 2013 Apr; 34(4): 521-528.
Impact Factor: 0.38
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Calce E, Monfregola L, Sandomenico A, Saviano M, De Luca S
* Fluorescence study for selecting specific ligands toward HER2 receptor: An example of receptor fragment approach (565 views)
Eur J Med Chem (ISSN: 0223-5234, 1768-3254, 1768-3254electronic), 2013 Mar; 61: 116-121.
Impact Factor: 3.432
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Causa F, Della Moglie R, Iaccino E, Mimmi S, Marasco D, Scognamiglio PL, Battista E, Palmieri C, Cosenza C, Sanguigno L, Quinto I, Scala G, Netti PA
* Evolutionary screening and adsorption behavior of engineered M13 bacteriophage and derived dodecapeptide for selective decoration of gold interfaces (542 views)
J Colloid Interface Sci (ISSN: 0021-9797, 1095-7103electronic, 0021-9797linking), 2013 Jan 1; 389(1): 220-229.
Impact Factor: 1.646
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Rosa R, Marciano R, Malapelle U, Formisano L, Nappi L, D'Amato C, D'Amato V, Damiano V, Marf G, Del Vecchio S, Zannetti A, Greco A, De Stefano A, Carlomagno C, Veneziani BM, Troncone G, De Placido S, Bianco R
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Impact Factor: 8.193
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Lanzillo R, Quarantelli M, Bonavita S, Ventrella G, Lus G, Vacca G, Prinster A, Orefice G, Tedeschi G, Brescia Morra V
* Natalizumab vs interferon beta 1a in relapsing-remitting multiple sclerosis: A head-to-head retrospective study (485 views)
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Impact Factor: 2.474
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Tagliamonte M, Marasco D, Ruggiero A, de Stradis A, Tornesello ML, Totrov M, Buonaguro FM, Buonaguro L
* HIV p24 as Scaffold for Presenting Conformational HIV Env Antigens (598 views)
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Impact Factor: 3.73
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Risitano AM, Imbriaco M, Marando L, Seneca E, Soscia E, Malcovati L, Iori AP, Pane F, Notaro R, Matarazzo M
* From perpetual haemosiderinuria to possible iron overload: iron redistribution in paroxysmal nocturnal haemoglobinuria patients on eculizumab by magnetic resonance imaging (431 views)
Br J Haematol (ISSN: 0007-1048, 0007-1048linking, 1365-2141electronic), 2012 Sep; 158(3): 415-418.
Impact Factor: 4.942
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Pedicini P, Caivano R, Jereczek-Fossa BA, Strigari L, Vischioni B, Alterio D, Cremonesi M, Botta F, Nappi A, Improta G, Storto G, Benassi M, Orecchia R
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Impact Factor: 1.461
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Troise F, Monti M, Merlino A, Cozzolino F, Fedele C, Russo Krauss I, Sica F, Pucci P, D'Alessio G, De Lorenzo C
* A novel ErbB2 epitope targeted by human antitumor immunoagents (505 views)
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Impact Factor: 3.79
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Lanzillo R, Orefice G, Prinster A, Ventrella G, Liuzzi R, Scarano V, Florio C, Vacca G, Brunetti A, Alfano B, Brescia Morra V, Bonavita V
* Predictive factors of neutralizing antibodies development in relapsing-remitting multiple sclerosis patients on interferon Beta-1b therapy (395 views) (PDF 241 views)
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Impact Factor: 1.315
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Palmieri C, Falcone C, Iaccino E, Tuccillo FM, Gaspari M, Trimboli F, De Laurentiis A, Luberto L, Pontoriero M, Pisano A, Vecchio E, Fierro O, Panico M, Larobina M, Gargiulo S, Costa N, Dal Piaz F, Schiavone M, Arra C, Giudice A, Palma G, Barbieri A, Quinto I, Scala G
* In vivo targeting and growth inhibition of the A20 murine B-cell lymphoma by an idiotype-specific peptide binder (786 views)
Blood (ISSN: 0006-4971, 0006-6497, 0390-6078), 2010 Jul 15; 116(2): 226-238.
Impact Factor: 10.558
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Alberobello AT, D'Esposito V, Marasco D, Doti N, Ruvo M, Bianco R, Tortora G, Esposito I, Fiory F, Miele C, Beguinot F, Formisano P
* Selective Disruption of Insulin-like Growth Factor-1 (IGF-1) Signaling via Phosphoinositide-dependent Kinase-1 Prevents the Protective Effect of IGF-1 on Human Cancer Cell Death (728 views)
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Impact Factor: 5.328
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Storto G, De Renzo A, Pellegrino T, Perna F, De Falco T, Erra P, Nardelli A, Speranza A, Klain M, Rotoli B, Pace L
* Assessment of Metabolic Response to Radioimmunotherapy with (90)Y-Ibritumomab Tiuxetan in Patients with Relapsed or Refractory B-Cell Non-Hodgkin Lymphoma (505 views)
Radiology (ISSN: 0033-8419, 1527-1315, 0033-8419linking), 2010 Jan; 254(1): 245-252.
Impact Factor: 6.069
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Sciacca MFM, Carbone V, Pappalardo M, Milardi D, La Rosa C, Grasso DM
* Interaction of human amylin with phosphatidylcholine and phosphatidylserine membranes (438 views)
Mol Cryst Liq Cryst (ISSN: 1542-1406), 2009; 500: 73-81.
Impact Factor: 0.451
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Paladino A, Costantini S, Colonna G, Facchiano AM
Molecular modelling of miraculin: Structural analyses and functional hypotheses (27 views)
Biochem Biophys Res Commun (ISSN: 0006-291xlinking), 2008 Feb 29; 367 (1 ): 26-32.
Impact Factor:
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Palmieri G, Marino M, Salvatore M, Budillon A, Meo G, Caraglia M, Montella L
* Cetuximab is an active treatment of metastatic and chemorefractory thymoma (500 views)
Frontiers In Bioscience (ISSN: 1093-9946, 1093-4715), 2007 Jan 1; 12(2): 757-761.
Impact Factor: 2.989
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Sciacca MFM, Milardi D, Pappalardo M, La Rosa C, Grasso DM
* Role of electrostatics in the thermal stability of ubiquitin: A combined DSC and MM study (334 views)
Journal Of Thermal Analysis And Calorimetry (ISSN: 1388-6150), 2006 Nov; 86(2): 311-314.
Impact Factor: 1.438
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Verdoliva A, Marasco D, De Capua A, Saporito A, Bellofiore P, Manfredi V, Fattorusso R, Pedone C, Ruvo M
* A new ligand for immunoglobulin G subdomains by screening of a synthetic peptide library (614 views)
Chembiochem (ISSN: 1439-4227, 1439-7633, 1439-7633electronic), 2005 Jul; 6(7): 1242-1253.
Impact Factor: 3.94
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Herga S, Brutus A, Vitale RM, Miche H, Perrier J, Puigserver A, Scaloni A, Giardina T
* Site-directed mutagenesis and molecular modelling studies show the role of Asp82 and cysteines in rat acylase 1, a member of the M20 family (458 views)
Biochem Biophys Res Commun (ISSN: 0006-291xprint, 0006-291xlinking), 2005 May 6; 330(2): 540-546.
Impact Factor: 3
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Esposito G, Vitagliano L, Costanzo P, Borrelli L, Barone R, Pavone L, Izzo P, Zagari A, Salvatore F
* Human aldolase A natural mutants: relationship between flexibility of the C-terminal region and enzyme function (476 views)
Biochem J (ISSN: 0264-6021, 1470-8728electronic, 0264-6021linking), 2004 May 15; 380: 51-56.
Impact Factor: 4.278
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Saviano M, Improta R, Benedetti E, Carrozzini B, Cascarano GL, Didierjean C, Toniolo C, Crisma M
* Benzophenone photophore flexibility and proximity: Molecular and crystal-state structure of a Bpa-containing trichogin dodecapeptide analogue (511 views)
Chembiochem (ISSN: 1439-4227, 1439-7633, 1439-7633electronic), 2004 Apr 2; 5(4): 541-544.
Impact Factor: 3.474
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Russo M, Palumbo R, Mupo A, Tosto M, Iacomino G, Scognamiglio A, Tedesco I, Galano G, Russo GL
* Flavonoid quercetin sensitizes a CD95-resistant cell line to apoptosis by activating protein kinase Cα (735 views)
Oncogene (ISSN: 0950-9232, 1476-5594), 2003; 22(21): 3330-3342.
Impact Factor: 6.495
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Wiesehan K, Buder K, Linke RP, Patt S, Stoldt M, Unger E, Schmitt B, Bucci E, Willbold D
* Selection of D-amino-acid peptides that bind to Alzheimer's disease amyloid peptide Aβ1-42 by mirror image phage display (876 views)
Chembiochem (ISSN: 1439-4227, 1439-7633, 1439-7633electronic), 2003; 4(8): 748-753.
Impact Factor: 3.992
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Fassina G, Ruvo M, Palombo G, Verdoliva A, Marino M
* Novel ligands for the affinity-chromatographic purification of antibodies (1505 views)
J Biochem Biophys Methods (ISSN: 0165-022x, 0165-022xlinking), 2001 Oct 30; 49(1-3): 481-490.
Impact Factor: 1.218
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Fonti R, Levchenko A, Mehta BM, Zhang J, Tsuruo T, Larson SM
Measurement of P-glycoprotein expression in human neuroblastoma xenografts using in vitro quantitative autoradiography (378 views)
Nucl Med Biol (ISSN: 0969-8051, 0883-2897), 1999 Jan; 26(1): 35-41.
Impact Factor: 1.623
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Monti SM, Fogliano V, Randazzo G, Peluso G, Logrieco A, Ritieni A
Polyclonal antibodies against fusaproliferin (361 views)
Can J Microbiol (ISSN: 0008-4166), 1999 Jan; 45(1): 45-50.
Impact Factor: 1.072
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Fonti R, Cheung N-KV, Bridger GJ, Guo H-F, Abrams MJ, Larson SM
99mTc-monoclonal antibody radiolabeled via hydrazino nicotinamide derivative for imaging disialoganglioside G(D2)-positive tumors (549 views)
Nucl Med Biol (ISSN: 0969-8051, 0883-2897), 1999; 26(6): 681-686.
Impact Factor: 1.623
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Renda A, Iovino F, Capasso L, Ricciardelli L, Tammaro V, Acampa W
Radioimmunoguided surgery in colorectal cancer: A 6-year experience with four different technical solutions (401 views)
Semin Surg Oncol Wiley Liss Inc (ISSN: 8756-0437), 1998 Dec; 15(4): 226-230.
Impact Factor: 1.045
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Pace L, Catalano L, Pinto A, De Renzo A, Di Gennaro F, Califano C, Del Vecchio S, Rotoli B, Salvatore M
Different patterns of technetium-99m sestamibi uptake in multiple myeloma (481 views)
Eur J Nucl Med (ISSN: 0340-6997, 1619-7070, 1619-7089), 1998 Jul; 25(7): 714-720.
Impact Factor: 2.564
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Levchenko A, Mehta B, Spengler BA, Narkar A, Fonti R, Biedler JL, Tsuruo T, Larson SM
Measurement of P-glycoprotein expression in multidrug-resistant human neuroblastoma cell lines using self-competitive binding assay (368 views)
Anal Biochem (ISSN: 0003-2697, 1096-0309electronic, 0003-2697linking), 1996 May 1; 236(2): 338-343.
Impact Factor: 2.017
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Li PY, Del Vecchio S, Fonti R, Carriero MV, Potena MI, Botti G, Miotti S, Lastoria S, Menard S, Colnaghi MI, Salvatore M
Local concentration of folate binding protein GP38 in sections of human ovarian carcinoma by in vitro quantitative autoradiography (545 views)
J Nucl Med (ISSN: 0161-5505, 1535-5667, 1535-5667electronic), 1996 Apr; 37(4-6): 665-672.
Impact Factor: 3.491
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Carriero MV, Franco P, Del Vecchio S, Massa O, Botti G, D'Aiuto G, Stoppelli MP, Salvatore M
Tissue distribution of soluble and receptor-bound urokinase in human breast cancer using a panel of monoclonal antibodies (565 views)
Cancer Res (ISSN: 0008-5472, 1538-7445, 1538-7445electronic), 1994 Oct 15; 54(20): 5445-5454.
Impact Factor: 8.426
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Caraglia M, Tagliaferri P, Correale P, Genua G, Pinto A, Del Vecchio S, Esposito G, Bianco AR
CYTOSINE-ARABINOSIDE INCREASES THE BINDING OF I-125 LABELED EPIDERMAL GROWTH-FACTOR AND I-125 TRANSFERRIN AND ENHANCES THE IN-VITRO TARGETING OF HUMAN TUMOR-CELLS WITH ANTI-(GROWTH FACTOR-RECEPTOR) MAB (364 views)
Cancer Immunol Immunother (ISSN: 0340-7004, 0340-7004linking), 1993 Sep; 37(3): 150-156.
Impact Factor: 1.269
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Caraglia M, Tagliaferri P, Correale P, Genua G, Pinto A, Del Vecchio S, Esposito G, Bianco AR
Cytosine arabinoside increases the binding of 125I-labelled epidermal growth factor and 125I-transferrin and enhances the in vitro targeting of human tumour cells with anti-(growth factor receptor) mAb (341 views)
Cancer Immunol Immunother (ISSN: 0340-7004linking), 1993 Aug; 37 (3 ): 150-156.
Impact Factor: 4.293
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D'Aiuto G, Del Vecchio S, Mansi L, D'Aprile M, Botti G, Salvatore M
Malignant melanoma of the nipple: A case studied with radiolabeled monoclonal antibody (728 views)
Tumori (ISSN: 0300-8916, 0300-8916print, 0300-8916linking), 1991 Oct 31; 77(5): 449-451.
Impact Factor: 0.408
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Manna R, Salvatore M, Di Leo MA, Scuderi F, Greco AV, Ghirlanda G, Gambassi G
Relationship between urinary neopterin excretion and islet cell antibodies in type 1 (insulin-dependent) diabetes (596 views)
Diabetes Res (ISSN: 0265-5985), 1991 May; 17(1): 33-36.
Impact Factor: 0
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Camera L, Del Vecchio S, Petrillo A, Esposito G, Frasci G, Iaffaioli RV, Bianco AR, Salvatore M
Evaluation of therapeutic response using iodine-131-B72. 3 monoclonal antibody in patients with ovarian carcinoma (603 views)
Eur J Nucl Med (ISSN: 0340-6997, 1619-7070, 1619-7089), 1991 Apr; 18(4): 269-273.
Impact Factor: 2.692
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Del Vecchio S, Camera L, Petrillo A, Esposito G, Frasci G, Iaffaioli RV, Bianco AR, Salvatore M
Clinical studies with B72. 3 in ovarian cancer and probe-guided tumor localization (763 views)
Nucl Med Biol (ISSN: 0883-2897, 0969-8051), 1991; 18(1): 85-87.
Impact Factor: 1.042
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Del Vecchio S, Mansi L, Petrillo A, Camera L, Sofia M, Marra A, Carratú L, Salvatore M
Endobronchial administration of iodine-131 B72. 3 monoclonal antibody in patients with lung cancer (349 views)
Eur J Nucl Med (ISSN: 0340-6997, 1619-7070, 1619-7089), 1991; 18(2): 129-132.
Impact Factor: 2.692
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Papa V, Reese CC, Brunetti A, Vigneri R, Siiteri PK, Goldfine ID
Progestins increase insulin receptor content and insulin stimulation of growth in human breast carcinoma cells (593 views)
Cancer Res (ISSN: 0008-5472, 1538-7445, 1538-7445electronic), 1990 Dec 15; 50(24): 7858-7862.
Impact Factor: 8.426
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Valenza V, Maussier ML, D'Errico G, Lemmo GF, Frontera D, Ferrante A, Saletnich I, Valli A, Quarantelli M
Cancer of the pancreas and the periampullary region: The usefulness of tumor marker products with the monoclonal antibody technique (378 views)
J Nucl Med Allied Sci (ISSN: 0392-0208), 1990 Oct; 34(4SUPPL.): 131-134.
Impact Factor: 1.91
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Del Vecchio S, Reynolds JC
NONUNIFORM DISTRIBUTION OF RADIOLABELED MONOCLONAL-ANTIBODY IN TUMOR (337 views)
J Nucl Med (ISSN: 0161-5505, 1535-5667, 1535-5667electronic), 1990 Jul; 31(7): 1252-1252.
Impact Factor: 3.491
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Steis RG, Carrasquillo JA, Mccabe R, Bookman MA, Reynolds JC, Larson SM, Smith JW, Clark JW, Dailey V, Del Vecchio S
Toxicity, immunogenicity, and tumor radioimmunodetecting ability of two human monoclonal antibodies in patients with metastatic colorectal carcinoma (367 views)
J Clin Oncol (ISSN: 0732-183x, 0732-183xlinking), 1990 Mar; 8(3): 476-490.
Impact Factor: 7.878
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Trischitta V, Wong KY, Brunetti A, Scalisi R, Vigneri R, Goldfine ID
Endocytosis, recycling, and degradation of the insulin receptor. Studies with monoclonal antireceptor antibodies that do not activate receptor kinase (366 views)
Jbc Papers (ISSN: 0021-9258, 1083-351x, 0021-9258linking), 1989 Mar 25; 264(9): 5041-5046.
Impact Factor: 6.963
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d'Onofrio G, Menichella G, Zini G, Rumi C, Pierelli L, Quarantelli M, Puggioni PL, Mango G
Lymphocyte subset counting by immunoperoxidase using an automated routine hematologic analyzer (477 views)
Haematologica (ISSN: 0390-6078, 0006-4971, 0006-6497), 1989 Mar; 74(2): 137-142.
Impact Factor: 2.56
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Reynolds JC, Del Vecchio S, Sakahara H, Lora ME, Carrasquillo JA, Neumann RD, Larson SM
ANTI-MURINE ANTIBODY RESPONSE TO MOUSE MONOCLONAL ANTIBODIES CLINICAL FINDINGS AND IMPLICATIONS (922 views)
Nucl Med Biol (ISSN: 0969-8051, 0883-2897), 1989; 16(2): 121-125.
Impact Factor: 1.042
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Salvatore M, Lastoria S, Del Vecchio S, Mansi L
Detection of colon cancer with radiolabelled monoclonal antibodies (460 views)
Nucl Med Biol (ISSN: 0883-2897, 0969-8051), 1989; 16(2): 103-104.
Impact Factor: 1.042
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Del Vecchio S, Reynolds JC, Carrasquillo JA, Blasberg RG, Neumann RD, Lotze MT, Bryant GJ, Farkas RJ, Larson SM
Local distribution and concentration of intravenously injected 131I-9. 2. 27 monoclonal antibody in human malignant melanoma (426 views)
Cancer Res (ISSN: 0008-5472, 1538-7445, 1538-7445electronic), 1989; 49(10): 2783-2789.
Impact Factor: 8.426
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Manna R, Salvatore M, Scuderi F, Papa G, Marietti G, Greco AV, Ghirlanda G, Gambassi G
Negative correlation between ICA persistence and beta cell restoration after IDDM diagnosis (407 views)
Diabetes Res (ISSN: 0265-5985), 1988 Nov; 9(3): 101-103.
Impact Factor: 0
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Del Vecchio S, Reynolds JC, Blasberg RG, Neumann RD, Carrasquillo JA, Hellström I, Larson SM
Measurement of local Mr 97, 000 and 250, 000 protein antigen concentration in sections of human melanoma tumor using in vitro quantitative autoradiography (569 views)
Cancer Res (ISSN: 0008-5472, 1538-7445, 1538-7445electronic), 1988; 48(19): 5475-5481.
Impact Factor: 8.426
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74 Records (71 excluding Abstracts).
Total impact factor: 254.517 (245.835 excluding Abstracts).
Total 5 year impact factor: 270.793 (259.497 excluding Abstracts).
Total impact factor: 254.517 (245.835 excluding Abstracts).
Total 5 year impact factor: 270.793 (259.497 excluding Abstracts).
230 views. Last view on Thursday 10 October 2024, 20:02:47
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