A recent update on the use of microbial transglutaminase for the generation of biotherapeutics (166 views)
World J Microbiol Biotechnol (ISSN: 0959-3993linking), 2020 Mar 14; 36(4): 53-53.
Export to BibTeX Export to EndNote
Paper type: Journal Article, Review,
Impact factor: 2.477, 5-year impact factor: 0
Url: Not available.
Keywords: Bacteria Enzymology, Bacterial Proteins Metabolism, Biocatalysis, Biotechnology, Fungal Proteins Metabolism, Fungi Enzymology, Immunoconjugates Metabolism, Protein Engineering Methods, Substrate Specificity, Transglutaminases Metabolism, Antibody–drug Conjugate, Chemoenzymatic Reactions, Microbial Transglutaminase, Protein Bioconjugation,
Affiliations:
*** IBB - CNR ***
Institute of Biostructure and Bioimaging, CNR (IBB-CNR), Via Mezzocannone, 16, 80134, Naples, Italy. nunzianna.doti@cnr.it.
Institute of Crystallography, CNR (IC-CNR), c/o Area Science Park s.s. 14 Km 163.5, Basovizza, 34149, Trieste, Italy.
Department of Environmental, Biological and Pharmaceutical Sciences and Technologies (DiSTABIF), University L. Vanvitelli, Via Vivaldi, 43, 80100, Caserta, Italy.
BioVIIIx R&D, Via B. Brin, 59C, 80142, Naples, Italy.
References:
Not available.
World J Microbiol Biotechnol (ISSN: 0959-3993linking), 2020 Mar 14; 36(4): 53-53.
Export to BibTeX Export to EndNote
Paper type: Journal Article, Review,
Impact factor: 2.477, 5-year impact factor: 0
Url: Not available.
Keywords: Bacteria Enzymology, Bacterial Proteins Metabolism, Biocatalysis, Biotechnology, Fungal Proteins Metabolism, Fungi Enzymology, Immunoconjugates Metabolism, Protein Engineering Methods, Substrate Specificity, Transglutaminases Metabolism, Antibody–drug Conjugate, Chemoenzymatic Reactions, Microbial Transglutaminase, Protein Bioconjugation,
Affiliations:
*** IBB - CNR ***
Institute of Biostructure and Bioimaging, CNR (IBB-CNR), Via Mezzocannone, 16, 80134, Naples, Italy. nunzianna.doti@cnr.it.
Institute of Crystallography, CNR (IC-CNR), c/o Area Science Park s.s. 14 Km 163.5, Basovizza, 34149, Trieste, Italy.
Department of Environmental, Biological and Pharmaceutical Sciences and Technologies (DiSTABIF), University L. Vanvitelli, Via Vivaldi, 43, 80100, Caserta, Italy.
BioVIIIx R&D, Via B. Brin, 59C, 80142, Naples, Italy.
References:
Not available.
A recent update on the use of microbial transglutaminase for the generation of biotherapeutics
The recent scientific progresses on the use of enzyme-mediated reactions in organic, non-aqueous and aqueous media have significantly supported the growing demand of new biotechnological and/or pharmacological products. Today, a plethora of microbial enzymes, used as biocatalysts, are available. Among these, microbial transglutaminases (MTGs) are broadly used for their ability to catalyse the formation of an isopeptide bond between the γ-amide group of glutamines and the ε-amino group of lysine. Due to their promiscuity towards primary amine-containing substrates and the more stringent specificity for glutamine-containing peptide sequences, several combined approaches can be tailored for different settings, making MTGs very attractive catalysts for generating protein-protein and protein small molecule's conjugates. The present review offers a recent update on the modifications attainable by MTG-catalysed bioreactions as reported between 2014 and 2019. In particular, we present a detailed and comparative overview on the MTG-based methods for proteins and antibodies engineering, with a particular outlook on the synthesis of homogeneous antibody-drug conjugates.
The recent scientific progresses on the use of enzyme-mediated reactions in organic, non-aqueous and aqueous media have significantly supported the growing demand of new biotechnological and/or pharmacological products. Today, a plethora of microbial enzymes, used as biocatalysts, are available. Among these, microbial transglutaminases (MTGs) are broadly used for their ability to catalyse the formation of an isopeptide bond between the γ-amide group of glutamines and the ε-amino group of lysine. Due to their promiscuity towards primary amine-containing substrates and the more stringent specificity for glutamine-containing peptide sequences, several combined approaches can be tailored for different settings, making MTGs very attractive catalysts for generating protein-protein and protein small molecule's conjugates. The present review offers a recent update on the modifications attainable by MTG-catalysed bioreactions as reported between 2014 and 2019. In particular, we present a detailed and comparative overview on the MTG-based methods for proteins and antibodies engineering, with a particular outlook on the synthesis of homogeneous antibody-drug conjugates.
A recent update on the use of microbial transglutaminase for the generation of biotherapeutics
| ▼ Design and screening of molecular repertoires as a source for new bioactive compounds |
A recent update on the use of microbial transglutaminase for the generation of biotherapeutics
Other filter: ([btitle,keywords] "Bacteria Enzymology" ...
Baker S, Gilhen-baker M, Roviello GN
* The Role of Nutrition and Forest-Bathing in the Physical Rehabilitation of Physically Inactive Patients: From the Molecular Aspects to New Nature-Inspired Techniques (150 views)
International Journal Of Environmental Research And Public Health, 2023 Jan; 20: 793-805.
Impact Factor: 4.614
View Export to BibTeX Export to EndNote
Berisio R, Delogu G
* PGRS domain structures: Doomed to sail the mycomembrane (127 views)
Plos Pathog (ISSN: 1553-7366linking), 2022 Sep 1; 18(9): e1010760-N/D.
Impact Factor: 7.5
View Export to BibTeX Export to EndNote
Squeglia F, Marasco D, Ruggiero A, Testa G, Esposito L, Berisio R
* Structural and Binding Properties of the Active Cell Wall Hydrolase RipA from M. tuberculosis, a Promising Biosensing Molecule for Early Warning Bacterial Detection (86 views)
Curr Med Chem (ISSN: 0929-8673linking, 1875-533xelectronic), 2022; 29(24): 4282-4292.
Impact Factor: 4.74
View Export to BibTeX Export to EndNote
De Simone G, Di Fiore A, Truppo E, Langella E, Vullo D, Supuran CT, Monti SM
* Exploration of the residues modulating the catalytic features of human carbonic anhydrase XIII by a site-specific mutagenesis approach (296 views)
J Enzym Inhib Med Ch (ISSN: 1475-6366linking, 1475-6374electronic), 2019 Dec; 34(1): 1506-1510.
Impact Factor: 4.027
View Export to BibTeX Export to EndNote
Caporale A, Monti A, Selis F, Sandomenico A, Tonon G, Ruvo M, Doti N
* A comparative analysis of catalytic activity and stability of microbial transglutaminase in controlled denaturing conditions (218 views)
J Biotechnol (ISSN: 0168-1656linking), 2019 Aug 20; 302: 48-57.
Impact Factor: 3.163
View Export to BibTeX Export to EndNote
Squeglia F, Moreira M, Ruggiero A, Berisio R
* The Cell Wall Hydrolytic NlpC/P60 Endopeptidases in Mycobacterial Cytokinesis: A Structural Perspective (362 views)
Cells (ISSN: 2073-4409linking, 2073-4409electronic), 2019 Jun 18; 8(6): N/D-N/D.
Impact Factor: 5.7
View Export to BibTeX Export to EndNote
Zambrano G, Ruggiero E, Malafronte A, Chino M, Maglio O, Pavone V, Nastri F, Lombardi A
* Artificial Heme Enzymes for the Construction of Gold-Based Biomaterials (454 views)
Int J Mol Sc (ISSN: 1422-0067electronic, 1422-0067linking, 1661-6596), 2018 Sep 24; 19(10): N/D-N/D.
Impact Factor: 3.687
View Export to BibTeX Export to EndNote
Squeglia F, Berisio R, Ruggiero A
* The novel thermostable cellulose-degrading enzyme DtCel5H from Dictyoglomus thermophilum: crystallization and X-ray crystallographic analysis (416 views)
Acta Crystallogr F Struct Biol Commun (ISSN: 2053-230xelectronic, 2053-230xlinking), 2018 Jan 1; 74(Pt1): 1-5.
Impact Factor: 0.8
View Export to BibTeX Export to EndNote
Caserta G, Chino M, Firpo V, Zambrano G, Leone L, D'Alonzo D, Nastri F, Maglio O, Pavone V, Lombardi A
* Enhancement of Peroxidase Activity in Artificial Mimochrome VI Catalysts through Rational Design (344 views)
Chembiochem, 2018; 19: 1823-1826.
Impact Factor: 2.774
View Export to BibTeX Export to EndNote
Alterio V, Langella E, De Simone G, Monti SM
* Cadmium-containing carbonic anhydrase CDCA1 in marine diatom Thalassiosira weissflogii (848 views)
Mar Drugs (ISSN: 1660-3397, 1660-3397linking), 2015 Mar 25; 13(4): 1688-1697.
Impact Factor: 3.345
View Export to BibTeX Export to EndNote
Caporale A, Selis F, Pain D, Sandomenico A, Jotti S, Tonon G, Ruvo M
* The LQSP tetrapeptide is a new highly efficient substrate of microbial transglutaminase for the site-specific derivatization of peptides and proteins (820 views)
Biotechnol J (ISSN: 1860-6768), 2015 Jan; 10(1): 154-161.
Impact Factor: 3.781
View Export to BibTeX Export to EndNote
Galdiero S, Falanga A, Cantisani M, Vitiello M, Morelli G, Galdiero M
* Peptide-Lipid Interactions: Experiments and Applications (1417 views)
Int J Mol Sc (ISSN: 1422-0067, 1661-6596, 1422-0067electronic), 2013 Sep; 14(9): 18758-18789.
Impact Factor: 2.339
View Export to BibTeX Export to EndNote
Berisio R
* Learning from pathogens: Exploiting host-pathogen liaison for therapeutic development (682 views)
Curr Protein Pept Sci (ISSN: 1389-2037, 1389-2037linking), 2012 Dec; 13(8): 697-698.
Impact Factor: 2.326
View Export to BibTeX Export to EndNote
De Simone G, Supuran CT
* (In)organic anions as carbonic anhydrase inhibitors (520 views)
J Chem Res (ISSN: 0162-0134, 1873-3344, 0162-0134print), 2012 Jun; 111: 117-129.
Impact Factor: 3.197
View Export to BibTeX Export to EndNote
Limauro D, D'Ambrosio K, Langella E, De Simone G, Galdi I, Pedone C, Pedone E, Bartolucci S
* Exploring the catalytic mechanism of the first dimeric Bcp: Functional, structural and docking analyses of Bcp4 from Sulfolobus solfataricus (566 views)
Biochimie (ISSN: 1638-6183, 0300-9084, 1638-6183electronic), 2010 Oct; 92(10): 1435-1444.
Impact Factor: 3.787
View Export to BibTeX Export to EndNote
Castellano I, Merlino A, Rossi M, La Cara F
* Biochemical and structural properties of gamma-glutamyl transpeptidase from Geobacillus thermodenitrificans: An enzyme specialized in hydrolase activity (748 views)
Biochimie (ISSN: 0300-9084, 1638-6183, 1638-6183electronic), 2010 May; 92(5): 464-474.
Impact Factor: 3.787
View Export to BibTeX Export to EndNote
Moccia M, Musumeci D, Roviello GN, Fusco S, Valente M, Bucci EM, Sapio R, Pedonea C, Netti PA
* Preliminary studies on noncovalent hyperbranched polymers based on PNA and DNA building blocks (1225 views)
J Pept Sci (ISSN: 1075-2617, 1099-1387, 1075-2617print), 2009 Oct; 15(10): 647-653.
Impact Factor: 1.807
View Export to BibTeX Export to EndNote
Roviello GN, Musumeci D, Moccia M, Castiglione M, Cesarani A, Bucci EM, Saviano M, Pedone C, Benedetti E
* Evidences Of Complex Formation Between Daba-Based Nucleo-Gamma-Peptides With Alternate Configuration Backbone (638 views)
J Pept Sci (ISSN: 1075-2617, 1099-1387, 1075-2617print), 2009 Mar; 15(3): 147-154.
Impact Factor: 1.807
View Export to BibTeX Export to EndNote
Lucignani G, Salvatore M
* Biotechnology in molecular and cellular imaging (476 views)
Minerva Biotecnol (ISSN: 1120-4826), 2009; 21(1): 1-3.
Impact Factor: 0.268
View Export to BibTeX Export to EndNote
Roviello GN, Moccia M, Sapio R, Valente M, Bucci EM, Castiglione M, Pedone C, Perretta G, Benedetti E, Musumeci D
* Synthesis, Characterization And Hybridization Studies Of New Nucleo-Gamma-Peptides Based On Diaminobutyric Acid (614 views)
J Pept Sci (ISSN: 1075-2617, 1099-1387, 1075-2617print), 2006 Dec; 12(12): 829-835.
Impact Factor: 1.801
View Export to BibTeX Export to EndNote
Galdiero S, Vitiello M, Amodeo P, D'Isanto M, Cantisani M, Pedone C, Galdiero M
* Structural requirements for proinflammatory activity of porin P2 loop 7 from Haemophilus influenzae (685 views)
Biochemistry (ISSN: 0006-2960, 1520-4995, 1520-4995electronic), 2006 Apr 11; 45(14): 4491-4501.
Impact Factor: 3.633
View Export to BibTeX Export to EndNote
Grasso G, D'Agata R, Rizzarelli E, Spoto G, D'Andrea LD, Pedone C, Picardi A, Romanelli A, Fragai M, Yeo KJ
* Activity of anchored human matrix metalloproteinase-1 catalytic domain on Au(111) surfaces monitored by ESI-MS (706 views)
J Mass Spectrom (ISSN: 1076-5174, 1096-9888), 2005 Dec; 40(12): 1565-1571.
Impact Factor: 3.574
View Export to BibTeX Export to EndNote
Fassina G, Ruvo M, Palombo G, Verdoliva A, Marino M
* Novel ligands for the affinity-chromatographic purification of antibodies (1508 views)
J Biochem Biophys Methods (ISSN: 0165-022x, 0165-022xlinking), 2001 Oct 30; 49(1-3): 481-490.
Impact Factor: 1.218
View Export to BibTeX Export to EndNote
Gomez Palacios LR, Bracamonte AG
Development of nano- and microdevices for the next generation of biotechnology, wearables and miniaturized instrumentation (58 views)
Rsc Adv (ISSN: 2046-2069electronic), N/D; 12 (20 ): 12806-12822.
Impact Factor: 3.84
View Export to BibTeX Export to EndNote
* The Role of Nutrition and Forest-Bathing in the Physical Rehabilitation of Physically Inactive Patients: From the Molecular Aspects to New Nature-Inspired Techniques (150 views)
International Journal Of Environmental Research And Public Health, 2023 Jan; 20: 793-805.
Impact Factor: 4.614
View Export to BibTeX Export to EndNote
Berisio R, Delogu G
* PGRS domain structures: Doomed to sail the mycomembrane (127 views)
Plos Pathog (ISSN: 1553-7366linking), 2022 Sep 1; 18(9): e1010760-N/D.
Impact Factor: 7.5
View Export to BibTeX Export to EndNote
Squeglia F, Marasco D, Ruggiero A, Testa G, Esposito L, Berisio R
* Structural and Binding Properties of the Active Cell Wall Hydrolase RipA from M. tuberculosis, a Promising Biosensing Molecule for Early Warning Bacterial Detection (86 views)
Curr Med Chem (ISSN: 0929-8673linking, 1875-533xelectronic), 2022; 29(24): 4282-4292.
Impact Factor: 4.74
View Export to BibTeX Export to EndNote
De Simone G, Di Fiore A, Truppo E, Langella E, Vullo D, Supuran CT, Monti SM
* Exploration of the residues modulating the catalytic features of human carbonic anhydrase XIII by a site-specific mutagenesis approach (296 views)
J Enzym Inhib Med Ch (ISSN: 1475-6366linking, 1475-6374electronic), 2019 Dec; 34(1): 1506-1510.
Impact Factor: 4.027
View Export to BibTeX Export to EndNote
Caporale A, Monti A, Selis F, Sandomenico A, Tonon G, Ruvo M, Doti N
* A comparative analysis of catalytic activity and stability of microbial transglutaminase in controlled denaturing conditions (218 views)
J Biotechnol (ISSN: 0168-1656linking), 2019 Aug 20; 302: 48-57.
Impact Factor: 3.163
View Export to BibTeX Export to EndNote
Squeglia F, Moreira M, Ruggiero A, Berisio R
* The Cell Wall Hydrolytic NlpC/P60 Endopeptidases in Mycobacterial Cytokinesis: A Structural Perspective (362 views)
Cells (ISSN: 2073-4409linking, 2073-4409electronic), 2019 Jun 18; 8(6): N/D-N/D.
Impact Factor: 5.7
View Export to BibTeX Export to EndNote
Zambrano G, Ruggiero E, Malafronte A, Chino M, Maglio O, Pavone V, Nastri F, Lombardi A
* Artificial Heme Enzymes for the Construction of Gold-Based Biomaterials (454 views)
Int J Mol Sc (ISSN: 1422-0067electronic, 1422-0067linking, 1661-6596), 2018 Sep 24; 19(10): N/D-N/D.
Impact Factor: 3.687
View Export to BibTeX Export to EndNote
Squeglia F, Berisio R, Ruggiero A
* The novel thermostable cellulose-degrading enzyme DtCel5H from Dictyoglomus thermophilum: crystallization and X-ray crystallographic analysis (416 views)
Acta Crystallogr F Struct Biol Commun (ISSN: 2053-230xelectronic, 2053-230xlinking), 2018 Jan 1; 74(Pt1): 1-5.
Impact Factor: 0.8
View Export to BibTeX Export to EndNote
Caserta G, Chino M, Firpo V, Zambrano G, Leone L, D'Alonzo D, Nastri F, Maglio O, Pavone V, Lombardi A
* Enhancement of Peroxidase Activity in Artificial Mimochrome VI Catalysts through Rational Design (344 views)
Chembiochem, 2018; 19: 1823-1826.
Impact Factor: 2.774
View Export to BibTeX Export to EndNote
Alterio V, Langella E, De Simone G, Monti SM
* Cadmium-containing carbonic anhydrase CDCA1 in marine diatom Thalassiosira weissflogii (848 views)
Mar Drugs (ISSN: 1660-3397, 1660-3397linking), 2015 Mar 25; 13(4): 1688-1697.
Impact Factor: 3.345
View Export to BibTeX Export to EndNote
Caporale A, Selis F, Pain D, Sandomenico A, Jotti S, Tonon G, Ruvo M
* The LQSP tetrapeptide is a new highly efficient substrate of microbial transglutaminase for the site-specific derivatization of peptides and proteins (820 views)
Biotechnol J (ISSN: 1860-6768), 2015 Jan; 10(1): 154-161.
Impact Factor: 3.781
View Export to BibTeX Export to EndNote
Galdiero S, Falanga A, Cantisani M, Vitiello M, Morelli G, Galdiero M
* Peptide-Lipid Interactions: Experiments and Applications (1417 views)
Int J Mol Sc (ISSN: 1422-0067, 1661-6596, 1422-0067electronic), 2013 Sep; 14(9): 18758-18789.
Impact Factor: 2.339
View Export to BibTeX Export to EndNote
Berisio R
* Learning from pathogens: Exploiting host-pathogen liaison for therapeutic development (682 views)
Curr Protein Pept Sci (ISSN: 1389-2037, 1389-2037linking), 2012 Dec; 13(8): 697-698.
Impact Factor: 2.326
View Export to BibTeX Export to EndNote
De Simone G, Supuran CT
* (In)organic anions as carbonic anhydrase inhibitors (520 views)
J Chem Res (ISSN: 0162-0134, 1873-3344, 0162-0134print), 2012 Jun; 111: 117-129.
Impact Factor: 3.197
View Export to BibTeX Export to EndNote
Limauro D, D'Ambrosio K, Langella E, De Simone G, Galdi I, Pedone C, Pedone E, Bartolucci S
* Exploring the catalytic mechanism of the first dimeric Bcp: Functional, structural and docking analyses of Bcp4 from Sulfolobus solfataricus (566 views)
Biochimie (ISSN: 1638-6183, 0300-9084, 1638-6183electronic), 2010 Oct; 92(10): 1435-1444.
Impact Factor: 3.787
View Export to BibTeX Export to EndNote
Castellano I, Merlino A, Rossi M, La Cara F
* Biochemical and structural properties of gamma-glutamyl transpeptidase from Geobacillus thermodenitrificans: An enzyme specialized in hydrolase activity (748 views)
Biochimie (ISSN: 0300-9084, 1638-6183, 1638-6183electronic), 2010 May; 92(5): 464-474.
Impact Factor: 3.787
View Export to BibTeX Export to EndNote
Moccia M, Musumeci D, Roviello GN, Fusco S, Valente M, Bucci EM, Sapio R, Pedonea C, Netti PA
* Preliminary studies on noncovalent hyperbranched polymers based on PNA and DNA building blocks (1225 views)
J Pept Sci (ISSN: 1075-2617, 1099-1387, 1075-2617print), 2009 Oct; 15(10): 647-653.
Impact Factor: 1.807
View Export to BibTeX Export to EndNote
Roviello GN, Musumeci D, Moccia M, Castiglione M, Cesarani A, Bucci EM, Saviano M, Pedone C, Benedetti E
* Evidences Of Complex Formation Between Daba-Based Nucleo-Gamma-Peptides With Alternate Configuration Backbone (638 views)
J Pept Sci (ISSN: 1075-2617, 1099-1387, 1075-2617print), 2009 Mar; 15(3): 147-154.
Impact Factor: 1.807
View Export to BibTeX Export to EndNote
Lucignani G, Salvatore M
* Biotechnology in molecular and cellular imaging (476 views)
Minerva Biotecnol (ISSN: 1120-4826), 2009; 21(1): 1-3.
Impact Factor: 0.268
View Export to BibTeX Export to EndNote
Roviello GN, Moccia M, Sapio R, Valente M, Bucci EM, Castiglione M, Pedone C, Perretta G, Benedetti E, Musumeci D
* Synthesis, Characterization And Hybridization Studies Of New Nucleo-Gamma-Peptides Based On Diaminobutyric Acid (614 views)
J Pept Sci (ISSN: 1075-2617, 1099-1387, 1075-2617print), 2006 Dec; 12(12): 829-835.
Impact Factor: 1.801
View Export to BibTeX Export to EndNote
Galdiero S, Vitiello M, Amodeo P, D'Isanto M, Cantisani M, Pedone C, Galdiero M
* Structural requirements for proinflammatory activity of porin P2 loop 7 from Haemophilus influenzae (685 views)
Biochemistry (ISSN: 0006-2960, 1520-4995, 1520-4995electronic), 2006 Apr 11; 45(14): 4491-4501.
Impact Factor: 3.633
View Export to BibTeX Export to EndNote
Grasso G, D'Agata R, Rizzarelli E, Spoto G, D'Andrea LD, Pedone C, Picardi A, Romanelli A, Fragai M, Yeo KJ
* Activity of anchored human matrix metalloproteinase-1 catalytic domain on Au(111) surfaces monitored by ESI-MS (706 views)
J Mass Spectrom (ISSN: 1076-5174, 1096-9888), 2005 Dec; 40(12): 1565-1571.
Impact Factor: 3.574
View Export to BibTeX Export to EndNote
Fassina G, Ruvo M, Palombo G, Verdoliva A, Marino M
* Novel ligands for the affinity-chromatographic purification of antibodies (1508 views)
J Biochem Biophys Methods (ISSN: 0165-022x, 0165-022xlinking), 2001 Oct 30; 49(1-3): 481-490.
Impact Factor: 1.218
View Export to BibTeX Export to EndNote
Gomez Palacios LR, Bracamonte AG
Development of nano- and microdevices for the next generation of biotechnology, wearables and miniaturized instrumentation (58 views)
Rsc Adv (ISSN: 2046-2069electronic), N/D; 12 (20 ): 12806-12822.
Impact Factor: 3.84
View Export to BibTeX Export to EndNote
24 Records (24 excluding Abstracts).
Total impact factor: 77.515 (77.515 excluding Abstracts).
Total 5 year impact factor: 72.05 (72.05 excluding Abstracts).
Total impact factor: 77.515 (77.515 excluding Abstracts).
Total 5 year impact factor: 72.05 (72.05 excluding Abstracts).
166 views. Last view on Wednesday 09 October 2024, 7:00:12
ibb.cnr.it
