Dual assessment of kidney perfusion and pH by exploiting a dynamic CEST-MRI approach in an acute kidney ischemia-reperfusion injury murine model (74 views)
Nmr Biomed (ISSN: 0952-3480linking, 1099-1492electronic), 2020 Jun; 33(6): e4287-N/D.
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Paper type: Journal Article, Research Support, Non-U. S. Gov'T,
Impact factor: 3.221, 5-year impact factor: 3.372
Url: Not available.
Keywords: Aki, Cest, Dce-Mri, Acute Kidney Injury, Iopamidol, Ischemia-Reperfusion Injury,
Affiliations:
*** IBB - CNR ***
Università degli Studi della Campania "Luigi Vanvitelli", Napoli, Italy.
Istituto di Biostrutture e Bioimmagini (IBB), Consiglio Nazionale delle Ricerche (CNR), Torino, Italy.
Centro di Imaging Molecolare, Dipartimento di Biotecnologie Molecolari e Scienze per la Salute, Università degli Studi di Torino, Torino, Italy.
Computer Assisted Clinical Medicine, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
References:
Not available.
Nmr Biomed (ISSN: 0952-3480linking, 1099-1492electronic), 2020 Jun; 33(6): e4287-N/D.
Export to BibTeX Export to EndNote
Paper type: Journal Article, Research Support, Non-U. S. Gov'T,
Impact factor: 3.221, 5-year impact factor: 3.372
Url: Not available.
Keywords: Aki, Cest, Dce-Mri, Acute Kidney Injury, Iopamidol, Ischemia-Reperfusion Injury,
Affiliations:
*** IBB - CNR ***
Università degli Studi della Campania "Luigi Vanvitelli", Napoli, Italy.
Istituto di Biostrutture e Bioimmagini (IBB), Consiglio Nazionale delle Ricerche (CNR), Torino, Italy.
Centro di Imaging Molecolare, Dipartimento di Biotecnologie Molecolari e Scienze per la Salute, Università degli Studi di Torino, Torino, Italy.
Computer Assisted Clinical Medicine, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
References:
Not available.
Dual assessment of kidney perfusion and pH by exploiting a dynamic CEST-MRI approach in an acute kidney ischemia-reperfusion injury murine model
Several factors can lead to acute kidney injury, but damage following ischemia and reperfusion injuries is the main risk factor and usually develops into chronic disease. MRI has often been proposed as a method with which to assess renal function. It does so by measuring the renal perfusion of an injected Gd-based contrast agent. The use of pH-responsive agents as part of the CEST (chemical exchange saturation transfer)-MRI technique has recently shown that pH homeostasis is also an important indicator of kidney functionality. However, there is still a need for methods that can provide more than one type of information following the injection of a single contrast agent for the characterization of renal function. Herein we propose, for the first time, dynamic CEST acquisition following iopamidol injection to quantify renal function by assessing both perfusion and pH homeostasis. The aim of this study is to assess renal functionality in a murine unilateral ischemia-reperfusion injury model at two time points (3 and 7 days) after acute kidney injury. The renal-perfusion estimates measured with iopamidol were compared with those obtained with a gadolinium-based agent, via a dynamic contrast enhanced (DCE)-MRI approach, to validate the proposed method. Compared with the contralateral kidneys, the clamped ones showed a significant decrease in renal perfusion, as measured using the DCE-MRI approach, which is consistent with reduced filtration capability. Dynamic CEST-MRI findings provided similar results, indicating that the clamped kidneys displayed significantly reduced renal filtration that persisted up to 7 days after the damage. In addition, CEST-MRI pH imaging showed that the clamped kidneys displayed significantly increased pH values, reflecting the disturbance to pH homeostasis. Our results demonstrate that a single CEST-MRI contrast agent can provide multiple types of information related to renal function and can discern healthy kidneys from pathological ones by combining perfusion measurements with renal pH mapping.
Several factors can lead to acute kidney injury, but damage following ischemia and reperfusion injuries is the main risk factor and usually develops into chronic disease. MRI has often been proposed as a method with which to assess renal function. It does so by measuring the renal perfusion of an injected Gd-based contrast agent. The use of pH-responsive agents as part of the CEST (chemical exchange saturation transfer)-MRI technique has recently shown that pH homeostasis is also an important indicator of kidney functionality. However, there is still a need for methods that can provide more than one type of information following the injection of a single contrast agent for the characterization of renal function. Herein we propose, for the first time, dynamic CEST acquisition following iopamidol injection to quantify renal function by assessing both perfusion and pH homeostasis. The aim of this study is to assess renal functionality in a murine unilateral ischemia-reperfusion injury model at two time points (3 and 7 days) after acute kidney injury. The renal-perfusion estimates measured with iopamidol were compared with those obtained with a gadolinium-based agent, via a dynamic contrast enhanced (DCE)-MRI approach, to validate the proposed method. Compared with the contralateral kidneys, the clamped ones showed a significant decrease in renal perfusion, as measured using the DCE-MRI approach, which is consistent with reduced filtration capability. Dynamic CEST-MRI findings provided similar results, indicating that the clamped kidneys displayed significantly reduced renal filtration that persisted up to 7 days after the damage. In addition, CEST-MRI pH imaging showed that the clamped kidneys displayed significantly increased pH values, reflecting the disturbance to pH homeostasis. Our results demonstrate that a single CEST-MRI contrast agent can provide multiple types of information related to renal function and can discern healthy kidneys from pathological ones by combining perfusion measurements with renal pH mapping.
Dual assessment of kidney perfusion and pH by exploiting a dynamic CEST-MRI approach in an acute kidney ischemia-reperfusion injury murine model
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Dual assessment of kidney perfusion and pH by exploiting a dynamic CEST-MRI approach in an acute kidney ischemia-reperfusion injury murine model
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Pohlmann A, Back SJ, Fekete A, Friedli I, Hectors S, Jerome NP, Ku MC, Longo DL, Meier M, Millward JM, Periquito JS, Seeliger E, Serai SD, Waiczies S, Sourbron S, Laustsen C, Niendorf T
* Recommendations for Preclinical Renal MRI: A Comprehensive Open-Access Protocol Collection to Improve Training, Reproducibility, and Comparability of Studies (90 views)
Methods Mol Biol (ISSN: 1064-3745linking, 1940-6029electronic), 2021; 2216: 3-23.
Impact Factor: 1.17
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Longo DL, Irrera P, Consolino L, Sun PZ, Mcmahon MT
* Renal pH Imaging Using Chemical Exchange Saturation Transfer (CEST) MRI: Basic Concept (196 views)
Methods Mol Biol (ISSN: 1064-3745linking, 1940-6029electronic), 2021; 2216: 241-256.
Impact Factor: 1.17
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Longo DL, Cutrin JC, Michelotti F, Irrera P, Aime S
* Noninvasive evaluation of renal pH homeostasis after ischemia reperfusion injury by CEST-MRI (439 views)
Nmr Biomed (ISSN: 0952-3480, 1099-1492electronic, 0952-3480linking), 2017; 30(7): N/D-N/D.
Impact Factor: 3.031
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De Chiara L, Fagoonee S, Ranghino A, Bruno S, Camussi G, Tolosano E, Silengo L, Altruda F
* Renal cells from spermatogonial germline stem cells protect against kidney injury (411 views)
J Am Soc Nephrol (ISSN: 1046-6673), 2014 Feb; 25(2): 316-328.
Impact Factor: 9.343
View Export to BibTeX Export to EndNote
* Recommendations for Preclinical Renal MRI: A Comprehensive Open-Access Protocol Collection to Improve Training, Reproducibility, and Comparability of Studies (90 views)
Methods Mol Biol (ISSN: 1064-3745linking, 1940-6029electronic), 2021; 2216: 3-23.
Impact Factor: 1.17
View Export to BibTeX Export to EndNote
Longo DL, Irrera P, Consolino L, Sun PZ, Mcmahon MT
* Renal pH Imaging Using Chemical Exchange Saturation Transfer (CEST) MRI: Basic Concept (196 views)
Methods Mol Biol (ISSN: 1064-3745linking, 1940-6029electronic), 2021; 2216: 241-256.
Impact Factor: 1.17
View Export to BibTeX Export to EndNote
Longo DL, Cutrin JC, Michelotti F, Irrera P, Aime S
* Noninvasive evaluation of renal pH homeostasis after ischemia reperfusion injury by CEST-MRI (439 views)
Nmr Biomed (ISSN: 0952-3480, 1099-1492electronic, 0952-3480linking), 2017; 30(7): N/D-N/D.
Impact Factor: 3.031
View Export to BibTeX Export to EndNote
De Chiara L, Fagoonee S, Ranghino A, Bruno S, Camussi G, Tolosano E, Silengo L, Altruda F
* Renal cells from spermatogonial germline stem cells protect against kidney injury (411 views)
J Am Soc Nephrol (ISSN: 1046-6673), 2014 Feb; 25(2): 316-328.
Impact Factor: 9.343
View Export to BibTeX Export to EndNote
4 Records (3 excluding Abstracts).
Total impact factor: 14.714 (5.371 excluding Abstracts).
Total 5 year impact factor: 21.435 (11.814 excluding Abstracts).
Total impact factor: 14.714 (5.371 excluding Abstracts).
Total 5 year impact factor: 21.435 (11.814 excluding Abstracts).
74 views. Last view on Wednesday 25 September 2024, 6:54:57
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