High Affinity vs. Native Fibronectin in the Modulation of αvβ3 Integrin Conformational Dynamics: Insights from Computational Analyses and Implications for Molecular Design
High Affinity vs. Native Fibronectin in the Modulation of αvβ3 Integrin Conformational Dynamics: Insights from Computational Analyses and Implications for Molecular Design(211 views) Paladino A, Civera M, Belvisi L, Colombo G
Paper type: Journal Article
, Research Support, Non-U. S. Gov'T
Impact factor: 0, 5-year impact factor: 0
Url: Not available.
Keywords: Binding Sites
, Drug Discovery Methods
, Fibronectins Chemistry Ultrastructure
, Integrin Alphavbeta3 Chemistry Ultrastructure
, Models, Chemical
, Molecular Dynamics Simulation
, Protein Binding
, Protein Conformation
, Protein Domains
Affiliations: Istituto di Chimica del Riconoscimento Molecolare CNR, Milan, Italy.
Dipartimento di Chimica, Università degli Studi di Milano, Milan, Italy.
References: Not available.
High Affinity vs. Native Fibronectin in the Modulation of αvβ3 Integrin Conformational Dynamics: Insights from Computational Analyses and Implications for Molecular Design
Understanding how binding events modulate functional motions of multidomain proteins is a major issue in chemical biology. We address several aspects of this problem by analyzing the differential dynamics of αvβ3 integrin bound to wild type (wtFN10, agonist) or high affinity (hFN10, antagonist) mutants of fibronectin. We compare the dynamics of complexes from large-scale domain motions to inter-residue coordinated fluctuations to characterize the distinctive traits of conformational evolution and shed light on the determinants of differential αvβ3 activation induced by different FN sequences. We propose an allosteric model for ligand-based integrin modulation: the conserved integrin binding pocket anchors the ligand, while different residues on the two FN10's act as the drivers that reorganize relevant interaction networks, guiding the shift towards inactive (hFN10-bound) or active states (wtFN10-bound). We discuss the implications of results for the design of integrin inhibitors.
High Affinity vs. Native Fibronectin in the Modulation of αvβ3 Integrin Conformational Dynamics: Insights from Computational Analyses and Implications for Molecular Design
High Affinity vs. Native Fibronectin in the Modulation of αvβ3 Integrin Conformational Dynamics: Insights from Computational Analyses and Implications for Molecular Design