Synthesis, Characterization And Hybridization Studies Of New Nucleo-Gamma-Peptides Based On Diaminobutyric Acid(670 views) Roviello GN, Moccia M, Sapio R, Valente M, Bucci EM, Castiglione M, Pedone C, Perretta G, Benedetti E, Musumeci D
Keywords: γ-Peptides, Daba, Dna Analogue, Amino Acid Derivative, C Peptide, Complementary Dna, Diagnostic Agent, Diaminobutyric Acid, Gamma Nucleopeptide, Natural Product, Nucleic Acid Base, Nucleoprotein, Prebiotic Agent, Article, Biotechnology, Chemical Bond, Chemical Modification, Chirality, Dna Binding, Enzyme Degradation, Hybridization, Nucleic Acid Probe, Oligomerization, Peptide Synthesis, Priority Journal, Protein Analysis, Protein Stability, Reaction Analysis, Base Sequence, Chromatography, High Pressure Liquid, Circular Dichroism, Molecular Conformation, Molecular Structure, Peptide Nucleic Acids, Spectrometry, Electrospray Ionization, Spectrophotometry, Ultraviolet, Temperature,
Affiliations: *** IBB - CNR ***
Istituto di Biostrutture e Bioimmagini-CNR, Via Mezzocannone 16, Napoli 80134, Italy
Università degli Studi di Napoli 'Federico ll', Dip. Scienze Biologiche sez. Biostrutture, via Mezzocannone 16, Napoli 80134, Italy
Bionucleon Srl, via Ribes 5, Colleretto Giacosa, TO 10010, Italy
Universit degli Studi di Napoli 'Federico ll', Dip. Scienze Biologiche sez. Biostrutture, via Mezzocannone 16, Napoli 80134, Italy
References: Not available.
Synthesis, Characterization And Hybridization Studies Of New Nucleo-Gamma-Peptides Based On Diaminobutyric Acid
In the present work, we report the synthesis and the characterization of a new chiral nucleoaminoacid, in which a diaminobutyric moiety is connected to the DNA nucleobase by an amidic bond, and its oligomerization to give the corresponding nucleo-gamma-peptide. The ability of this synthetic polymer to bind complementary DNA was studied in order to explore its possible use in antigene/antisense or diagnostic applications. Our interest in the presented DNA analogue was also supported by the importance of gamma-aminoacid-containing compounds in natural products of biological activity and by the known stability of gamma-peptides to enzymatic degradation. Furthermore, our work could contribute to the study of the role of nucleopeptides as prebiotic material in a PNA world that could successively lead to the actual DNA/RNA/protein world, as recently assumed. Copyright (c) 2006 European Peptide Society and John Wiley & Sons, Ltd
Synthesis, Characterization And Hybridization Studies Of New Nucleo-Gamma-Peptides Based On Diaminobutyric Acid
No results.
Synthesis, Characterization And Hybridization Studies Of New Nucleo-Gamma-Peptides Based On Diaminobutyric Acid