The crystal structure of an EST2 mutant unveils structural insights on the H group of the carboxylesterase/lipase family(839 views) De Simone G, Menchise V, Alterio V, Mandrich L, Rossi M, Manco G, Pedone C
Keywords: Crystal Structure, Domain Swapping, Hsl, Lipase, Thermophilic Carboxylesterase, Dimer, Esterase Ii, Mutant Protein, Triacylglycerol Lipase, Unclassified Drug, Amino Terminal Sequence, Article, Crystallography, Enzyme Binding, Enzyme Mechanism, Enzyme Specificity, Enzyme Structure, Enzyme Substrate, Isomerization, Priority Journal, Protein Domain, Protein Family, Site Directed Mutagenesis, Structure Analysis, Three Dimensional Imaging, Amino Acid Sequence, Bacillus, Binding Sites, Chromatography, X-Ray, Dimerization, Enzyme Stability, Models, Molecular, Site-Directed, Mutation, Protein Conformation, Protein Engineering, Protein Structure, Secondary, Tertiary, Substrate Specificity, Temperature, Water, Alicyclobacillus, Alicyclobacillus Acidocaldarius,
Affiliations: *** IBB - CNR ***
Dipto. Chim. Biol.-Sezione B., University of Naples Federico II, Via Mezzocannone 16, 80134 N., Italy
Bioindustry Park del Canavese, Via Ribes 5, Colleretto Giocosa I-10010, Italy, Italy
Ist. di Biochim. Delle Proteine-CNR, Via P. Castellino 111, 80131 Naples, Italy, Italy
Dipto. Chim. Biol. -Sezione B., University of Naples Federico II, Via Mezzocannone 16, 80134 N., Italy
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Ollis, D. L., Cheah, E., Cygler, M., Dijkstra, B., Frolow, F., Franken, S. M., The alpha/beta hydrolase fold (1992) Protein Eng., 5, pp. 197-211
Br nger, A. T., Adams, P. D., Clore, G. M., De Lano, W. L., Gros, P., Grosse-Kunstleve, R. W., Crystallography & NMR system: A new software suite for macromolecular structure determination (1998) Acta Crystallog. Sect. D, 54, pp. 905-921
Ali, Y. B., Chahinian, H., Petry, S., Muller, G., Carri re, F., Verger, R., Abousalham, A., Might the kinetic behavior of hormone-sensitive lipase reflect the absence of the lid domain? (2004) Biochemistry, 43, pp. 9298-9306
Contreras, J. A., Karlsson, M., Osterlund, T., Laurell, H., Svenson, A., Holm, C., Hormone sensitive lipase is structurally related to acetylcholinesterase, bile salt stimulated lipase, and several fungal lipases. Building of a three dimensional model for the catalytic domain of hormone-sensitive lipase (1996) J. Biol. Chem., 271, pp. 31426-31430
Laskowski, R. A., MacArthur, M. W., Moss, D. S., Thornton, J. M., PROCHECK: A program to check the stereochemical quality of protein structures (1993) J. Appl. Crystallog., 26, pp. 283-291
Bennett, M. J., Choe, S., Eisenberg, D., The refined structure of dimeric diphtheria toxin at 2. 0 resolution (1994) Protein Sci., 3, pp. 1444-1463
Story, R. M., Weber, I. T., Steitz, T. A., The structure of the E. coli recA protein monomer and polymer (1992) Nature, 355, pp. 318-325
Lewis, R. J., Muchov, K., Brannigan, J. A., Bar k, I., Leonard, G., Wilkinson, A. J., Domain swapping in sporulation response regulator Spo0A (2000) J. Mol. Biol., 297, pp. 757-770
Newcomer, M. E., Protein folding and three-dimensional domain swapping: A strained relationship? (2002) Curr. Opin. Struct. Biol., 12, pp. 48-53
Jones, T. A., Zou, J. Y., Cowan, S. W., Kjeldgaard, M., Improved methods for building protein models in electron density maps and the location of errors in these models (1991) Acta Crystallog. Sect. a, 47, pp. 110-119
Thompson, J. D., Higgins, D. G., Gibson, T. J., CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice (1994) Nucl. Acids Res., 22, pp. 4673-4680
The crystal structure of an EST2 mutant unveils structural insights on the H group of the carboxylesterase/lipase family