Retinoic acid receptors α, β and γ, and cellular retinol binding protein-I expression in breast fibrocystic disease and cancer(874 views) Pasquali D, Bellastella A, Valente A, Botti G, Capasso I, del Vecchio S, Salvatore M, Colantuoni V, Sinisi AA
Keywords: Estrogen Receptor, Gene Product, Messenger Rna, Progesterone Receptor, Receptor Subtype, Retinoic Acid Receptor, Retinol Binding Protein, Retinol Derivative, Adult, Aged, Article, Breast Carcinoma, Cancer Control, Clinical Article, Controlled Study, Drug Targeting, Fibrocystic Breast Disease, Human, Human Tissue, Priority Journal, Protein Induction, Receptor Upregulation, Reverse Transcription Polymerase Chain Reaction, Breast Neoplasms, Female, Middle Aged, Retinol-Binding Proteins, Genetic,
Affiliations: Istituto di Endocrinologia, Facoltá di Medicina, Seconda Universitá di Napoli, Naples, Italy
Ist. Naz. Tumori Fondazione G. P., Univ.́ di Napoli Federico II, Naples, Italy
Istituto di Medicina Nucleare, Univ.́ di Napoli Federico II, Naples, Italy
Dipto. Biochim. Biotecnologie M., Univ.́ di Napoli Federico II, Naples, Italy
Cattedra di Endocrinologia, Seconda Universita'di Napoli, via Pansini 5, 80131 Naples, Italy
References: Not available.
Retinoic acid receptors α, β and γ, and cellular retinol binding protein-I expression in breast fibrocystic disease and cancer
Retinoids seem to act as agents of chemoprevention and differentiation in breast diseases. Their action is mediated by nuclear receptors, retinoic acid receptors (RARα, RARβ, RARγ) and retinoid X receptors (RXRα, RXRβ, RXRγ,) and modulated by cellular retinol binding proteins (CRBP). There are few published data on CRBP expression. In this study, we evaluated the expression of RARα, β and γ and CRBP type I (CRBP-I) gene expression in fibrocystic disease (FD) and in breast cancer (BC), studying 14 FD and 20 BC surgical samples by reverse transcription (RT)-PCR. We also evaluated mRNA concentrations in cancer samples by a semiquantitative PCR method, co- amplifying RARα, RARβ and CRBP-I genes with an unrelated gene, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), as internal control. All benign and malignant breast tissues expressed RARα, β and γ, and CRBP-I mRNAs. A greater concentration of RARβ mRNA was detected in cancer tissues with lower oestrogen and progesterone receptor concentrations, whereas RARe was detected in variable concentrations that were not related to those of steroid receptors. The CRBP-I concentration was similar in all samples studied. We demonstrated that all three PARs and CRBP-I transcripts are expressed in FD, and that RARβ, RARγ and CRBP-I mRNAs also are present in BC tissues. This indicates that both malignant and benign breast tissues may be target for retinoids, justifying the use of natural and synthetic vitamin A derivatives in the chemoprevention of breast disease.
Retinoic acid receptors α, β and γ, and cellular retinol binding protein-I expression in breast fibrocystic disease and cancer
No results.
Retinoic acid receptors α, β and γ, and cellular retinol binding protein-I expression in breast fibrocystic disease and cancer