Vitronectin binding to urokinase receptor in human breast cancer(430 views) Carriero MV, Del Vecchio S, Franco P, Potena MI, Chiaradonna F, Botti G, Stoppelli MP, Salvatore M
Clin Cancer Res (ISSN: 1078-0432, 1078-0432linking, 1078-0432print), 1997 Sep; 3(8): 1299-1308.
Affiliations: Ist. Naz. Stud. e la Cura dei Tumori, Via M. Semmola, 80131 Napoli, Italy
Centro Medicina Nucleare, Consiglio Nazionale delle Ricerche, Universitá Federico II, Via Pansini 5, 80131 Napoli, Italy
Ist. Internazionale Genet. e Biofis., Via Marconi 10, 80131 Napoli, Italy
Istituto Nazionale Tumori, Via Jannelli 554, 80131 Napoli, Italy
References: Vassalli, J.D., The Urokinase Receptor (1994) Fibrinolysis, 8, pp. 172-18
Hynes, R.O., Integrins: Versatility, modulation, and signaling in cell adhesion (1992) Cell, 69, pp. 11-25
Dano, K., Behrendt, N., Brunner, N., Ellis, V., Ploug, M., Pyke, C., The urokinase receptor: Protein structure and role in plasminogen activation and cancer invasion (1994) Fibrinolysis, 8, pp. 189-203
Pepper, M.S., Vassalli, J.D., Montesano, R., Orci, L., Urokinase-type plasminogen activator is induced in migrating capillary endothelial cells (1987) J. Cell Biol., 105, pp. 2535-2541
Strickland, S., Reich, E., Scherman, M.I., Plasminogen activator in early embryogenesis: Enzyme production by trophoblast and parietal endoderm (1976) Cell, 15, pp. 393-403
Blasi, F., Vassalli, J.D., Dano, K., Urokinase-type plasminogen activator: Pro-enzyme, receptor, and inhibitors (1987) J. Cell Biol., 104, pp. 801-804
Camiolo, S.M., Markus, G., Evers, J.L., Plasminogen activator content of neoplastic and benign human prostate tissues: Fibrin augmentation of an activator activity (1981) Int. J. Cancer, 27, pp. 191-198
Grondahl-Hansen, J., Christensen, I.J., Rosenquist, C., Brunner, N., Mouridsen, H.T., Dano, K., Blichert-Toft, M., High levels of urokinase-type plasminogen activator and its inhibitor PAI-1 in cytosolic extracts of breast carcinomas are associated with poor prognosis (1993) Cancer Res., 53, pp. 2513-2521
Vassalli, J.D., Baccino, D., Belin, D., A cellular binding site for the Mr 55,000 form of the human plasminogen activator urokinase (1985) J. Cell Biol., 100, pp. 86-92
Stoppelli, M.P., Corti, A., Soffientini, A., Cassani, G., Blasi, F., Assoian, R.K., Differentiation-enhanced binding of the amino-terminal fragment of human urokinase plasminogen activator to a specific receptor on U937 monocytes (1985) Proc. Natl. Acad. Sci. USA, 82, pp. 4939-4943
Ellis, V., Behrendt, N., Dano, K., Plasminogen activation by receptor-bound urokinase (1991) J. Biol. Chem., 266, pp. 12752-12758
Stoppelli, M.P., Tacchetti, C., Cubellis, M.V., Corti, A., Hearing, V.J., Cassani, G., Appella, E., Blasi, F., Autocrine saturation of pro-urokinase receptors on human A431 cells (1986) Cell, 45, pp. 675-684
Kook, Y.H., Adamski, J., Zelent, A., Ossowski, L., The effect of antisense inhibition of urokinase receptor in human squamous cell carcinoma on malignancy (1994) EMBO J., 13, pp. 3983-3991
Pyke, C., Graem, N., Ralfkiaer, E., Ronne, E., Hoyer-Hansen, G., Brunner, N., Dano, K., Receptor for urokinase is present in tumor-associated macrophages in ductal breast carcinoma (1993) Cancer Res., 53, pp. 1911-1915
Bianchi, E., Cohen, R.L., Thor, A.T., Todd III, R.F., Mizukami, I.F., Lawrence, D.A., Ljung, B.M., Smith, H.S., The urokinase receptor is expressed in invasive breast cancer but not in normal breast tissue (1994) Cancer Res., 54, pp. 861-866
Foekens, J.A., Schmitt, M., Van Putten, W.L.J., Peters, H.A., Bontenbal, M., Janicke, F., Klijn, J.G.M., Prognostic value of urokinase-type plasminogen activator in 671 primary breast cancer patients (1992) Cancer Res., 52, pp. 6101-6105
Ronne, E., Hayer-Hansen, G., Brunner, N., Pedersen, H., Rank, F., Osborne, C.K., Clark, G.M., Grondahl-Hansen, J., Urokinase receptor in breast tissue extracts. Enzyme-linked immunosorbent assay with a combination of mono- And polyclonal antibodies (1995) Breast Cancer Res. Treat., 33, pp. 199-207
Pedersen, H., Brumer, N., Francis, D., Osterlind, K., Ronne, E., Hansen, H.H., Dano, K., Grondahl-Hansen, J., Prognostic impact of urokinase, urokinase receptor and type 1 plasminogen activator inhibitor in squamous and large cell lung cancer tissue (1994) Cancer Res., 54, pp. 4671-4675
Nielsen, L.S., Kellerman, G.M., Behrendt, N., Picone, R., Dano, K., Blasi, F., A 55,000-60,000 Mr receptor protein for urokinase-type-plasminogen activator (1988) J. Biol. Chem., 263, pp. 2358-2363
Myohanen, H.T., Stephens, R.W., Hedman, K., Tapiovaara, H., Ronne, E., Hoier-Hansen, G., Dano, K., Vaheri, A., Distribution and lateral mobility of the urokinase-receptor complex at the cell surface (1993) J. Histochem. Cytochem., 41, pp. 1291-1301
Franco, P., Iaccarino, C., Chiaradonna, F., Brandazza, A., Iavarone, C., Mastronicola, M.R., Stoppelli, M.P., Phosphorylation of human pro-urokinase on Ser138/303 impairs its receptor-dependent ability to promote myelomonocytic adherence and motility (1997) J. Cell Biol., 137, pp. 779-790
Waltz, D.A., Sailor, L.Z., Chapman, H.A., Cytokines induce urokinase-dependent adhesion of human myeloid cells (1993) J. Clin. Invest., 91, pp. 1541-1552
Wei, Y., Waltz, D.A., Rao, N., Drummond, R.J., Rosemberg, S., Chapman, H.A., Identification of the urokinase receptor as an adhesion receptor for vitronectin (1994) J. Biol. Chem., 269, pp. 32380-32388
Moser, T.L., Enghild, J.J., Pizzo, S.V., Stack, M.S., Specific binding of urinary-type plasminogen activator (u-PA) to vitronectin and its role in mediating u-PA-dependent adhesion of U937 cells (1995) Biochem. J., 307, pp. 867-873
Tomasini, B.R., Mosher, D.F., Conformational states of vitronectin: Preferential expression of an antigenic epitope when vitronectin is covalently and noncovalently complexed with thrombin-antithrombin III or treated with urea (1988) Blood, 72, pp. 903-912
Cheresh, D.A., Smith, J.W., Cooper, H.M., Quaranta, V., A novel vitronectin receptor integrin (αvβx) is responsible for distinct adhesive properties of carcinoma cells (1989) Cell, 57, pp. 59-69
Zutter, M.M., Mazoujian, G., Santoro, S.A., Decreased expression of integrin adhesive protein receptors in adenocarcinoma of the breast (1990) Am. J. Pathol., 137, pp. 863-870
Pollanen, J., Hedman, K., Nielsen, L.S., Vaheri, A., Ultrastructural localization of plasma membrane-associated urokinase-type plasminogen activator at focal contacts (1988) J. Cell Biol., 106, pp. 87-95
Ciambrone, G.J., Mc Keown-Longo, P., Vitronectin regulates the synthesis and localization of urokinase-type plasminogen activator in HT1080 Cells (1992) J. Biol. Chem., 267, pp. 13617-13622
Salonen, E.M., Vaheri, A., Pollanen, J., Stephens, R., Andreasen, P., Mayer, M., Dano, K., Ruoslahti, E., Interaction of plasminogen activator inhibitor (PAI-1) with vitronectin (1989) J. Biol. Chem., 264, pp. 6339-6343
Steingrimur, S., Lawrence, D.A., The serpin PAI-1 inhibits cell migration by blocking integrin αvβ3 binding to vitronectin (1996) Nature (Lond.), 383, pp. 441-443
Waltz, D.A., Chapman, H.A., Reversible cellular adhesion to vitronectin linked to urokinase receptor occupancy (1994) J. Biol. Chem., 269, pp. 14746-14750
Carriero, M.V., Franco, P., Del Vecchio, S., Massa, O., Botti, G., D'Aiuto, G., Stoppelli, M.P., Salvatore, M., Tissue distribution of soluble and receptor-bound urokinase in human breast cancer using a panel of monoclonal antibodies (1994) Cancer Res., 54, pp. 5445-5454
Nolli, M.L., Corti, A., Soffientini, A., Cassani, G., A monoclonal antibody that recognizes the receptor binding region of human urokinase-type plasminogen activator (1986) Thromb. Haemostasis, 56, pp. 214-218
Roldan, A., Cubellis, M.V., Masucci, M.T., Behrendt, N., Lund, L.R., Dano, K., Appella, E., Blasi, F., Cloning and expression of the receptor for human urokinase plasminogen activator, a central molecule in cell surface, plasmin dependent proteolysis (1990) EMBO J., 9, pp. 467-474
Tortora, G., Pepe, S., Bianco, C., Baldassarre, G., Budillon, A., Clair, T., Cho-Chung, Y.S., Ciardiello, F., The RIα subunit of protein kinase a controls serum dependency and entry into cell cycle of human mammary epithelial cells (1994) Oncogene, 9, pp. 3233-3240
Del Vecchio, S., Stoppelli, M.P., Carriero, M.V., Fonti, R., Massa, O., Li, P.Y., Botti, G., Salvatore, M., Human urokinase receptor concentration in malignant and benign breast tumors by in vitro quantitative autoradiography: Comparison with urokinase levels (1993) Cancer Res., 53, pp. 3198-3206
Bittorf, S.V., Williams, E.C., Mosher, D.F., Alteration of vitronectin (1993) J. Biol. Chem., 268, pp. 24838-24846
Christensen, L., Wiborg, Simonsen, A.C., Heegaard, C.W., Moestrup, S.K., Andersen, J.A., Andreasen, P.A., Immunohistochemical localization of urokinase plasminogen activator, urokinase plasminogen activator type-1 inhibitor, urokinase receptor and α2-macroglobulin receptor in human breast carcinomas (1996) Int. J. Cancer, 66, pp. 441-452
Kohn, E.C., Liotta, L.A., Molecular insights into cancer invasion: Strategies for prevention and intervention (1995) Cancer Res., 55, pp. 1856-1862
Cajot, J.F., Schleuning, W.D., Medcalf, R.L., Bamat, J., Testuz, J., Lieberman, L., Sordat, B., Mouse L cells expressing pro-urokinase-type plasminogen activator: Effects on extracellular matrix degradation and invasion (1989) J. Cell Biol., 109, pp. 915-925
Betsholtz, C., Westermark, E., Heldin, C.H., Coexpression of a PDGF-like growth factor and PDGF receptors in a human osteosarcoma cell line: Implications for autocrine receptor activation (1984) Cell, 39, pp. 447-457. , B
Coffey, R.J., Goustin, A.S., Soderquist, A.M., Shipley, G.D., Wolfshohl, J., Carpenter, G., Moses, H.L., Transforming growth factor α and β expression in human colon cancer lines: Implications for an autocrine model (1987) Cancer Res., 47, pp. 4590-4594
Pourmir, L., Epelbaum, J., Regional stimulatory and inhibitory effects of guanine nucleotides on 125I-galanin binding in rat brain: Relationship with the rate of occupancy of galanin receptors by endogeneous galanin (1992) Neuroscience, 49, pp. 829-847
Zutter, M.M., Krigman, H.R., Santoro, S.A., Altered integrin expression in adenocarcinoma of the breast (1993) Am. J. Pathol., 142, pp. 1439-1448
Glukhova, M., Koteliansky, V., Sastre, X., Thiery, J.P., Adhesion system in normal breast and in invasive carcinoma (1995) Am. J. Pathol., 146, pp. 706-716
Busso, N., Masur, S.K., Lazega, D., Waxman, S., Ossowski, L., Induction of cell migration by pro-urokinase binding to its receptor: Possible mechanism for signal transduction in human epithelial cells (1994) J. Cell Biol., 126, pp. 259-270
Vassalli, J. D., The Urokinase Receptor (1994) Fibrinolysis, 8, pp. 172-18
Hynes, R. O., Integrins: Versatility, modulation, and signaling in cell adhesion (1992) Cell, 69, pp. 11-25
Pepper, M. S., Vassalli, J. D., Montesano, R., Orci, L., Urokinase-type plasminogen activator is induced in migrating capillary endothelial cells (1987) J. Cell Biol., 105, pp. 2535-2541
Camiolo, S. M., Markus, G., Evers, J. L., Plasminogen activator content of neoplastic and benign human prostate tissues: Fibrin augmentation of an activator activity (1981) Int. J. Cancer, 27, pp. 191-198
Vassalli, J. D., Baccino, D., Belin, D., A cellular binding site for the Mr 55, 000 form of the human plasminogen activator urokinase (1985) J. Cell Biol., 100, pp. 86-92
Stoppelli, M. P., Corti, A., Soffientini, A., Cassani, G., Blasi, F., Assoian, R. K., Differentiation-enhanced binding of the amino-terminal fragment of human urokinase plasminogen activator to a specific receptor on U937 monocytes (1985) Proc. Natl. Acad. Sci. USA, 82, pp. 4939-4943
Stoppelli, M. P., Tacchetti, C., Cubellis, M. V., Corti, A., Hearing, V. J., Cassani, G., Appella, E., Blasi, F., Autocrine saturation of pro-urokinase receptors on human A431 cells (1986) Cell, 45, pp. 675-684
Kook, Y. H., Adamski, J., Zelent, A., Ossowski, L., The effect of antisense inhibition of urokinase receptor in human squamous cell carcinoma on malignancy (1994) EMBO J., 13, pp. 3983-3991
Foekens, J. A., Schmitt, M., Van Putten, W. L. J., Peters, H. A., Bontenbal, M., Janicke, F., Klijn, J. G. M., Prognostic value of urokinase-type plasminogen activator in 671 primary breast cancer patients (1992) Cancer Res., 52, pp. 6101-6105
Nielsen, L. S., Kellerman, G. M., Behrendt, N., Picone, R., Dano, K., Blasi, F., A 55, 000-60, 000 Mr receptor protein for urokinase-type-plasminogen activator (1988) J. Biol. Chem., 263, pp. 2358-2363
Myohanen, H. T., Stephens, R. W., Hedman, K., Tapiovaara, H., Ronne, E., Hoier-Hansen, G., Dano, K., Vaheri, A., Distribution and lateral mobility of the urokinase-receptor complex at the cell surface (1993) J. Histochem. Cytochem., 41, pp. 1291-1301
Waltz, D. A., Sailor, L. Z., Chapman, H. A., Cytokines induce urokinase-dependent adhesion of human myeloid cells (1993) J. Clin. Invest., 91, pp. 1541-1552
Wei, Y., Waltz, D. A., Rao, N., Drummond, R. J., Rosemberg, S., Chapman, H. A., Identification of the urokinase receptor as an adhesion receptor for vitronectin (1994) J. Biol. Chem., 269, pp. 32380-32388
Moser, T. L., Enghild, J. J., Pizzo, S. V., Stack, M. S., Specific binding of urinary-type plasminogen activator (u-PA) to vitronectin and its role in mediating u-PA-dependent adhesion of U937 cells (1995) Biochem. J., 307, pp. 867-873
Tomasini, B. R., Mosher, D. F., Conformational states of vitronectin: Preferential expression of an antigenic epitope when vitronectin is covalently and noncovalently complexed with thrombin-antithrombin III or treated with urea (1988) Blood, 72, pp. 903-912
Cheresh, D. A., Smith, J. W., Cooper, H. M., Quaranta, V., A novel vitronectin receptor integrin (v x) is responsible for distinct adhesive properties of carcinoma cells (1989) Cell, 57, pp. 59-69
Zutter, M. M., Mazoujian, G., Santoro, S. A., Decreased expression of integrin adhesive protein receptors in adenocarcinoma of the breast (1990) Am. J. Pathol., 137, pp. 863-870
Ciambrone, G. J., Mc Keown-Longo, P., Vitronectin regulates the synthesis and localization of urokinase-type plasminogen activator in HT1080 Cells (1992) J. Biol. Chem., 267, pp. 13617-13622
Salonen, E. M., Vaheri, A., Pollanen, J., Stephens, R., Andreasen, P., Mayer, M., Dano, K., Ruoslahti, E., Interaction of plasminogen activator inhibitor (PAI-1) with vitronectin (1989) J. Biol. Chem., 264, pp. 6339-6343
Waltz, D. A., Chapman, H. A., Reversible cellular adhesion to vitronectin linked to urokinase receptor occupancy (1994) J. Biol. Chem., 269, pp. 14746-14750
Carriero, M. V., Franco, P., Del Vecchio, S., Massa, O., Botti, G., D'Aiuto, G., Stoppelli, M. P., Salvatore, M., Tissue distribution of soluble and receptor-bound urokinase in human breast cancer using a panel of monoclonal antibodies (1994) Cancer Res., 54, pp. 5445-5454
Nolli, M. L., Corti, A., Soffientini, A., Cassani, G., A monoclonal antibody that recognizes the receptor binding region of human urokinase-type plasminogen activator (1986) Thromb. Haemostasis, 56, pp. 214-218
Bittorf, S. V., Williams, E. C., Mosher, D. F., Alteration of vitronectin (1993) J. Biol. Chem., 268, pp. 24838-24846
Kohn, E. C., Liotta, L. A., Molecular insights into cancer invasion: Strategies for prevention and intervention (1995) Cancer Res., 55, pp. 1856-1862
Cajot, J. F., Schleuning, W. D., Medcalf, R. L., Bamat, J., Testuz, J., Lieberman, L., Sordat, B., Mouse L cells expressing pro-urokinase-type plasminogen activator: Effects on extracellular matrix degradation and invasion (1989) J. Cell Biol., 109, pp. 915-925
Coffey, R. J., Goustin, A. S., Soderquist, A. M., Shipley, G. D., Wolfshohl, J., Carpenter, G., Moses, H. L., Transforming growth factor and expression in human colon cancer lines: Implications for an autocrine model (1987) Cancer Res., 47, pp. 4590-4594
Zutter, M. M., Krigman, H. R., Santoro, S. A., Altered integrin expression in adenocarcinoma of the breast (1993) Am. J. Pathol., 142, pp. 1439-1448
Vitronectin binding to urokinase receptor in human breast cancer
Functional assembly of the plasminogen-dependent proteolytic system on the cell surface requires multiple interactions involving urokinase (uPA), urokinase receptor (uPAR), plasminogen activator inhibitors, and other molecules that mediate cell migration and adhesion. We analyzed the in vitro interaction of uPAR-containing particulate cell fractions with the amino- terminal fragment (ATF) of human urokinase and the matrix-like form of vitronectin. Binding and cross-linking of 125I-labeled ATF to crude membrane extracts from LB6-19 mouse cells overexpressing human uPARs in the presence of 25 nM urea-denatured vitronectin led to the formation of M(r) 137,000, 92,000, and 82,000 covalent complexes. Immunoprecipitation of the preformed cross-linked 125I-labeled complexes with anti-vitronectin, anti- uPA, or anti-uPAR antibodies revealed that the M(r) 82,000 and 92,000 species do contain ATF and vitronectin and identified the M(r) 137,000 species as a ternary complex formed by ATF, uPAR, and vitronectin. A similar electrophoretic pattern was displayed by acid-pretreated membranes extracted from MCF-7 breast carcinoma or HT1080 fibrosarcoma cell lines, as well as a ductal breast carcinoma specimen; the latter exhibited complex formation at concentrations of vitronectin lower than 10 nM. Finally uPAR-vitronectin interaction was further documented by the decreased reactivity of an anti- uPAR polyclonal antibody to acid-pretreated sections of 10 breast carcinomas that had been preincubated with vitronectin. Our findings highlight the ability of uPAR to interact simultaneously with vitronectin and uPA in breast cancer, supporting a dynamic coupling of the molecular mechanisms underlying plasminogen-dependent matrix degradation and cell adhesion.
Vitronectin binding to urokinase receptor in human breast cancer
Santulli G, Cipolletta E, Sorriento D, Del Giudice C, Anastasio A, Monaco S, Maione AS, Condorelli G, Puca A, Trimarco B, Illario M, Iaccarino G * CaMK4 gene deletion induces hypertension(349 views) J Am Heart Assoc Journal Of The American Heart Association (ISSN: 2047-9980), 2012; 1(4): N/D-N/D. Impact Factor:2.882 ViewExport to BibTeXExport to EndNote
Cusanno F, Cisbani E, Colilli S, Fratoni R, Garibaldi F, Giuliani F, Gricia M, Lucentini M, Magliozzi ML, Santarivenere F, Torrioli S, Cinti MN, Pani R, Pellegrini R, Simonetti G, Schillaci O, Del Vecchio S, Salvatore M, Majewski S, De Vincentis G, Scopinaro F * Results of clinical trials with SPEM(278 views) Nucl Instrum Methods Phys Res Sect A, 2007 Feb 1; 497(1): 46-50. Impact Factor:3.221 ViewExport to BibTeXExport to EndNote
Aloj L, Aurilio M, Rinaldi V, D'Ambrosio L, Tesauro D, Peitl PK, Maina T, Mansi R, Von Guggenberg E, Joosten L, Sosabowski JK, Breeman WA, De Blois E, Koelewijn S, Melis M, Waser B, Beetschen K, Reubi JC, De Jong M * The EEE project(449 views) Proc Int Cosm Ray Conf Icrc Universidad Nacional Autonoma De Mexico, 2007; 5(HEPART2): 977-980. Impact Factor:0 ViewExport to BibTeXExport to EndNote