Local concentration of folate binding protein GP38 in sections of human ovarian carcinoma by in vitro quantitative autoradiography(450 views) Li PY, Del Vecchio S, Fonti R, Carriero MV, Potena MI, Botti G, Miotti S, Lastoria S, Menard S, Colnaghi MI, Salvatore M
Affiliations: Medicina Nucleare, II Facoltá di Medicina, Via S. Pansini, 5, 80131 Naples, Italy
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Antony, A. C., The biological chemistry of folate receptors (1992) Blood, 79, pp. 2807-282
Kamen, B. A., Wang, M. T., Streckfuss, A. J., Peryea, X., Anderson, R. G. W., Delivery of folates to the cytoplasm of MA104 cells is mediated by a surface membrane receptor that recycles (1988) J Biol Chem, 263, pp. 13602-13609
Luhrs, C. A., Slomiany, B. L., A human membrane-associated folate binding protein is anchored by a glycosylphosphatidylinositol tail (1989) J Biol Chem, 264, pp. 21446-21449
Weitman, S. D., Lark, R. H., Coney, L. R., Distribution of the folate receptor GP38 in normal and malignant cell lines and tissues (1992) Cancer Res, 52, pp. 3396-3401
Kamen, B. A., Capdevila, A., Receptor-mediated folate accumulation is regulated by the cellular folate content (1986) Proc Natl Acad Sci USA, 83, pp. 5983-5987
Kane, M. A., Elwood, P. C., Portillo, R. M., Influence of immunoreactive folate-binding proteins of extracellular folate concentration on cultured human cells (1988) J Clin Invest, 81, pp. 1398-1406
Coney, L. R., Tomassetti, A., Carayannopoulos, L., Cloning of a tumor-associated antigen: MOv18 and MOv19 antibodies recognize a folate binding protein (1991) Cancer Res, 51, pp. 6125-6132
Campbell, I. G., Jones, T. A., Foulkes, W. D., Trowsdale, J., Folate binding protein is a marker for ovarian carcinoma (1991) Cancer Res, 51, pp. 5329-5338
Mamovani, L. T., Miotti, S., Menard, S., Folate binding protein distribution in normal tissues and biological fluids from ovarian carcinoma patients as detected by the monoclonal antibodies MOv18 and MOv19 (1994) Eur J Cancer, 30, pp. 363-369
Weitman, S. D., Weinberg, A. G., Coney, L. R., Zurawski, V. R., Jennings, D. S., Kamen, B. A., Cellular localization of the folate receptor: Potential role in drug toxicity and folate homeostasis (1992) Cancer Res, 52, pp. 6708-6711
Molthoff, C. J. M., Buist, M. R., Kenemans, P., Pinedo, H. M., Boven, E., Experimental and clinical analysis of the characteristics of a chimeric monoclonal antibody, MOv18, reactive with an ovarian cancer-associated antigen (1992) J Nucl Med, 33, pp. 2000-2005
Bolhuis, R. L. H., Lamers, C. H. J., Goey, S. H., Adoptive immunotherapy of ovarian carcinoma with BS-MAb-targeted lymphocytes: A multicenter study (1992) Int J Cancer, 7 (SUPPL.), pp. 78-81
Westerhof, G. R., Jansen, G., Van Emmerik, N., Membrane transport of natural folates and antifolate compounds in murine L1210 leukemia cells, role of carrier- and receptor-mediated transport systems (1991) Cancer Res, 51, pp. 5507-5513
Calvert, A. H., Newell, D. R., Jackman, A. L., Recent preclinical and clinical studies with the thymidylate synthase inhibitor N10-propargyl-5, 8-dideazafolic acid (CB3717) (1987) Natl Cancer Inst Monogr, 5, pp. 213-218
Cantwell, B. M. J., Macaulay, V., Harris, A. L., Phase II study of the antifolate N10-propargyl-5, 8-dideazafolic acid (CB3717) in advanced breast cancer (1988) Eur J Cancer Clin Oncol, 24, pp. 733-736
Jackman, A. L., Newell, D. R., Jodrell, D. I., In vitro and in vivo studies with 2-desamino-2-CH3-N10-propargyl-5, 8-dideazafolate (ICI 198583), an inhibitor of thymidylate synthase (1989) Chemistry and Biology of Pieridines, pp. 1023-1026. , Curtius HC, Gishla S, Blau N, eds. Berlin: Walter de Gruyter & Co
Young, A. B., Frey, K. A., Agranoff, B. W., Receptor assay: In vitro and in vivo (1986) Positron Emission Tomography and Autoradiography: Principles and Applications for the Brain and Heart, pp. 73-111. , Phelps M, Mazziotta J, Schelbert H, eds. New York: Raven Press
Fraker, P. J., Speck, J. C., Protein and cell membrane iodinations with a sparingly soluble chloramide 1, 3, 4, 6 tetrachloro-3 alpha, 6 alpha-diphenylglycouril (1978) Biochim Biophys Res Commun, 80, pp. 49-857
Hsu, S. M., Raine, L., Fanger, H., Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: A comparison between ABC and unlabeled antibody (PAP) procedures (1981) J Histochem Cytochem, 29, pp. 577-580
Silva, C. M., Tully, D. B., Petch, L. A., Jewell, M. C., Cidlowski, J. A., Application of a protein-blotting procedure to the study of human glucocorticoid receptor interactions with DNA (1987) Proc Natl Acad Sci USA, 84, pp. 1744-1748
Shockley, T. R., Lin, K., Sung, C., A quantitative analysis of tumor specific monoclonal antibody uptake by human melanoma xenografts: Effects of antibody immunological properties and tumor antigen expression levels (1992) Cancer Res, 52, pp. 357-366
Ross, J. F., Chaudhuri, P. K., Ratnam, M., Differential regulation of folate receptor isoforms in normal and malignant tissues in vivo and in established cell lines (1994) Cancer, 73, pp. 2432-2443
Page, T. S., Owen, W. C., Price, K., Elwood, P. C., Expression of the human placental folate receptor transcripts is regulated in human tissues (1993) J Mol Biol, 229, pp. 1175-1183
Shen, F., Ross, J. F., Wang, X., Ratnam, M., Identification of a novel folate receptor, a truncated receptor, and receptor type in hematopoietic cells: CDNA cloning, expression, immunoreactivity, and tissue specificity (1994) Biochemistry, 33, pp. 1209-1215
Local concentration of folate binding protein GP38 in sections of human ovarian carcinoma by in vitro quantitative autoradiography
Folate binding protein (FBP) GP38 is a membrane-associated glycoprotein that mediates the intracellular transport of folates. The enhanced expression of FBP in ovarian carcinomas provided a rational basis for clinical studies with specific monoclonal antibodies and some newly synthesized antifolate drugs. Because the outcome of these clinical studies ultimately depends on the degree of FBP expression, we measured the local concentration of FBP using I-125-MOv18 monoclonal antibody and quantitative autoradiography. Methods: Tissue sections from 37 specimens of ovarian carcinoma and 13 nonmalignant ovaries were incubated with increasing concentrations of I-125-MOv18 with and without an excess of unlabeled antibody. Tissue-bound radioactivity was measured by quantitative autoradiography. Results: Folate binding protein was found to be overexpressed in 91% of nonmucinous ovarian carcinomas, with local concentrations ranging between 1.14 and 82.84 pmole/g, Adjacent tumor sections simultaneously studied with I-125-MOv18 and a I-125-labeled folio add derivative showed matching and superimposable regional distributions of bound radioactivity of the two radioligands, indicating that the antigen, specifically recognized by I-125-MOv18 in nonmucinous ovarian carcinomas, is capable of binding folates. Nonmalignant ovaries did not contain measurable amounts of antigen when assayed with (125)MOv18 but showed a limited and specific binding of the I-125-folic acid derivative to tissue sections, The autoradiographic findings were confirmed by testing sections from mixtures of antigen-positive and antigen-negative cells, by immunoperoxidase staining with MOv18 on tumor sections and by biochemical identification of FBP in membrane fractions from tissue samples. Conclusion: Folate binding protein is overexpressed up to 80-90-fold in nonmucinous ovarian carcinomas compared with nonmalignant ovaries. Quantitation of FBP may provide a useful tool in the design of clinical studies with specific monoclonal antibodies and certain antifolate drugs that enter the cell through FBP.
Local concentration of folate binding protein GP38 in sections of human ovarian carcinoma by in vitro quantitative autoradiography
No results.
Local concentration of folate binding protein GP38 in sections of human ovarian carcinoma by in vitro quantitative autoradiography