Solution and solid state structure of an aib-containing cyclodecapeptide inhibiting the cholate uptake in hepatocytes(1298 views) Rossi F, Saviano M, Di Talia P, Di Blasio B, Pedone C, Zanotti G, Mosca M, Saviano G, Tancredi T, Ziegler K, Benedetti E
Keywords: Conformation, Cyclic Decapeptides, Cyclolinopeptide A, X-Ray, Amino Acids, Biochemistry, Computer Simulation, Enzymes, Hydrogen Bonds, Molecular Dynamics, Molecular Structure, Nuclear Magnetic Resonance Spectroscopy, Solutions, Synthesis (chemical), X Ray Diffraction, Chocolate Uptake, Cyclodecapeptide, Hepatocyte, Physicochemical Techniques, Biopolymers, Cholic Acid, Peptide Derivative, Article, Liver Cell, Molecular Interaction, Protein Conformation, Protein Structure, Animals, Cultured, Chloroform, Crystallography, Hydrogen Bonding, Models, Temperature,
Affiliations: Dipartimento di Chimica, Ctro. Studio Biocristallog. del CNR, Universita di Napoli 'Federico II', Via Mezzocannone, 4, I-80134 Napoli, Italy
References: Not available.
Solution and solid state structure of an aib-containing cyclodecapeptide inhibiting the cholate uptake in hepatocytes
The conformational analysis of synthetic cyclodecapeptide c(Pro-Phr-Phe-Aib-Leu)(2) related to the cyclolinopeptide A, in the solid state and solution, has been carried out by I-ray diffraction and nmr spectroscopy. The structure of the monoclinic form obtained om methanol [ a = 11.351 (5) Angstrom, b = 27.455 (2) Angstrom, c = 12.716(8) Angstrom, beta = 99.65(3)degrees; space group P2(1),; Z = 2] shows the prescnce of six intramolecular NH ... CO hydrogen bonds, with formation of four turns (three of type I and one of type IIl) and two C-16 ring structures. All peptide units are trans. The solution structure, as found by nmr; indicates that, at room temperature, the peptide is conformationally homogeneous; the structure determined is perfectly symmetrical and topological similar to that found in the solid state. The cyclodecapeptide exhibits similar biological activity to cyclolinopeptide A. (C) 1997 John Wiley & Sons, Inc.
Solution and solid state structure of an aib-containing cyclodecapeptide inhibiting the cholate uptake in hepatocytes
Kállay C, Dávid A, Timári S, Nagy EM, Sanna D, Garribba E, Micera G, De Bona P, Pappalardo G, Rizzarelli E, Sóvágó I * Copper(II) complexes of rat amylin fragments(659 views) Dalton T (ISSN: 1477-9234, 1477-9226, 1477-9234electronic), 2011 Oct 14; 40(38): 9711-9721. Impact Factor:3.838 ViewExport to BibTeXExport to EndNote