Structural and enzymatic properties of an in vivo proteolytic form of PD-S2, type 1 ribosome-inactivating protein from seeds of Phytolacca dioica
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Structural and enzymatic properties of an in vivo proteolytic form of PD-S2, type 1 ribosome-inactivating protein from seeds of Phytolacca dioica (387 views)

Di Maro A, Berisio R, Ruggiero A, Tamburino R, Severino V, Zacchia E, Parente A

Biochem Biophys Res Commun (ISSN: 0006-291x), 2012 May 11; 421(3): 514-520.

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Paper type: Journal Article,

Impact factor: 2.406, 5-year impact factor: 2.5

Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84860689194&partnerID=40&md5=f12517afb49dfb88683dc49d25587059

Keywords: 3d Modeling, Circular Dichroic Spectroscopy, Phytolacca Dioica, Proteolytic Regulation, Ribosome-Inactivating Proteins, Phytolacca Dioica S2 Protein, Unclassified Drug, Vegetable Protein, Article, Controlled Study, Enzyme Activity, In Vitro Study, In Vivo Study, Nonhuman, Plant Seed, Pokeweed, Priority Journal, Protein Degradation, Protein Folding, Protein Synthesis, Purification, Seed Development, Thermostability, Amino Acids, Essential, Germination, Protein Biosynthesis, Protein Conformation, Protein Stability, Proteolysis, Ribosome Inactivating Proteins, Type 1,

Affiliations:
*** IBB - CNR ***

Dipartimento di Scienze della Vita, Seconda Università di Napoli, Via Vivaldi 43, I-81100 Caserta, Italy
Istituto di Biostrutture e Bioimmagini, C.N.R., Via Mezzocannone 16, I-80134 Napoli, Italy


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Structural and enzymatic properties of an in vivo proteolytic form of PD-S2, type 1 ribosome-inactivating protein from seeds of Phytolacca dioica

PD-S2, type 1 ribosome-inactivating protein from Phytolacca dioica L. seeds, is an N-β-glycosidase likely involved in plant defence. In this work, we purified and characterized an in vivo proteolytic form of PD-S2, named cutPD-S2. Spectroscopic characterization of cutPD-S2 showed that the proteolytic cleavage between Asn195 and Arg196 does not alter the protein fold, but significantly affects its thermal stability. Most importantly, the proteolytic cleavage induces a 370-fold decrease of PD-S2 capacity of inhibiting in vitro protein biosynthesis.Our data catch the turning point from a typical role of PD-S2 as a defence protein to that of supplier of essential amino acids during seedling development. © 2012 Elsevier Inc.
Structural and enzymatic properties of an in vivo proteolytic form of PD-S2, type 1 ribosome-inactivating protein from seeds of Phytolacca dioica

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Structural and enzymatic properties of an in vivo proteolytic form of PD-S2, type 1 ribosome-inactivating protein from seeds of Phytolacca dioica

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Severino V, Chambery A, Di Maro A, Marasco D, Ruggiero A, Berisio R, Giansanti F, Ippoliti R, Parente A
* The role of the glycan moiety on the structure-function relationships of PD-L1, type 1 ribosome-inactivating protein from P. dioica leaves (407 views)
Mol Biosyst (ISSN: 1742-206x), 2010; 6(3): 570-579.
Impact Factor: 3.825
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Ruggiero A, Di Maro A, Severino V, Chambery A, Berisio R
* Crystal structure of PD-L1, a ribosome inactivating protein from Phytolacca dioica L. leaves with the property to induce DNA cleavage (417 views)
Biopolymers (ISSN: 0006-3525, 0006-6352, 0006-3525print), 2009 Dec; 91(12): 1135-1142.
Impact Factor: 2.605
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Ruggiero A, Chambery A, Di Maro A, Parente A, Berisio R
* Atomic Resolution (1. 1 Angstrom) Structure Of The Ribosome-Inactivating Protein Pd-L4 From Phytolacca Dioica L. Leaves (457 views)
Proteins (ISSN: 0887-3585, 1097-0134, 1097-0134electronic), 2008 Apr; 71(1): 8-15.
Impact Factor: 3.419
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Chambery A, Pisante M, Di Maro A, Di Zazzo E, Ruvo M, Costantini S, Colonna G, Parente A
* Invariant Ser211 is involved in the catalysis of PD-L4, type I RIP from Phytolacca dioica leaves (366 views)
Proteins (ISSN: 0887-3585, 1097-0134, 1097-0134electronic), 2007 Apr 1; 67(1): 209-218.
Impact Factor: 3.354
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Ruggiero A, Chambery A, Di Maro A, Mastroianni A, Parente A, Berisio R
* Crystallization and preliminary X-ray diffraction analysis of PD-L1, a highly glycosylated ribosome inactivating protein with DNase activity (617 views)
Protein Pept Lett (ISSN: 0929-8665, 1875-5305), 2007; 14(4): 407-409.
Impact Factor: 1.097
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Ruggiero A, Chambery A, Di Maro A, Pisante M, Parente A, Berisio R
* Crystallization and preliminary x-ray diffraction analysis of PD-L4, a ribosome inactivating protein from Phytolacca dioica L. leaves (371 views)
Protein Pept Lett (ISSN: 0929-8665, 1875-5305), 2007; 14(1): 97-100.
Impact Factor: 1.097
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6 Records (6 excluding Abstracts).
Total impact factor: 15.397 (15.397 excluding Abstracts).
Total 5 year impact factor: 16.422 (16.422 excluding Abstracts).

Last modified by Rita Berisio on Sunday 12 July 2020, 13:14:56
387 views. Last view on Monday 07 August 2023, 14:31:21