Receptor fragment approach to the binding between CCK8 peptide and cholecystokinin receptors: A fluorescence study on type B receptor fragment CCKB-R (352-379)
Receptor fragment approach to the binding between CCK8 peptide and cholecystokinin receptors: A fluorescence study on type B receptor fragment CCKB-R (352-379)(361 views) De Luca S, Sanseverino M, Zocchi I, Pedone C, Morelli G, Ragone R
Keywords: Cholecystokinin Type B Receptor, Fluorescence Spectroscopy, Peptide-Receptor Binding, Surfactant Titration, Cck8 Peptides, Fluorescence Titrations, Receptor Fragment Approach, Binding Energy, Biological Membranes, Dissociation, Proteins, Spectroscopic Analysis, Polypeptides, Cholecystokinin A Receptor, Cholecystokinin B Receptor, Cholecystokinin Octapeptide, Cholecystokinin Receptor, Ligand, Solvent, Analytic Method, Article, Dissociation Constant, Drug Receptor Binding, Equilibrium Constant, Molecular Model, Reliability, Circular Dichroism, Peptide Fragments, Spectrometry,
Affiliations: *** IBB - CNR ***
CIRPEB, Dipartimento di Chimica Biologica, Università Federico II, Via Mezzocannone 6/8, Naples, I-80134, Italy
INBIOS srl, Via Olivetti 1, Pozzuoli (NA), I-80078, Italy
Dipto. di Biochimica e Biofisica, CRISCEB, Seconda Universita di Napoli, Via Costantinopoli 16, I-80138 Naples, Italy
References: Berthold, M., Bartfai, T., (1997) Neurochem Res, 22, pp. 1023-103
Ragone, R., De Luca, S., Tesauro, D., Pedone, C., Morelli, G., (2001) Biopolymers, 56, pp. 47-53
De Luca, S., Ragone, R., Bracco, C., Digilio, G., Aloj, L., Tesauro, D., Saviano, M., Morelli, G., (2003) ChemBioChem, 4, pp. 1176-1187
Edelhoch, H., (1967) Biochemistry, 6, pp. 1948-1954
Pace, C.N., Vajdos, F., Fee, L., Grimsley, G., Gray, T., (1995) Protein Sci, 4, pp. 2411-2423
Gill, S.C., Von Hippel, P.H., (1989) Anal Biochem, 182, pp. 319-326
Ragone, R., Colonna, G., Servillo, L., Irace, G., (1985) Photochem Photobiol, 42, pp. 505-508
Emerson, M.F., Holtzer, A., (1965) J Phys Chem, 69, pp. 3718-3721
Esposito, C., Colicchio, P., Facchiano, A., Ragone, R., (1998) J Colloid Interface Sci, 200, pp. 310-312
Lauterwein, J., Bösch, C., Brown, L.R., Wüthrich, K., (1979) Biochim Biophys Acta, 556, pp. 244-264
Brown, L.R., Bösch, C., Wüthrich, K., (1981) Biochim Biophys Acta, 642, pp. 296-312
Le Maire, M., Champeil, P., Møller, J.V., (2000) Biochim Biophys Acta, 1508, pp. 86-111
Robinson, N.C., Tanford, C., (1975) Biochemistry, 14, pp. 369-378
Grefrath, S.P., Reynolds, J.A., (1974) Proc Natl Acad Sci U S A, 71, pp. 3913-3916
Reynolds, J.A., Stoeckenius, W., (1977) Proc Natl Acad Sci U S A, 74, pp. 2803-2804
Bohm, G., Muhr, R., Jaenicke, R., (1992) Protein Eng, 5, pp. 191-195
Manning, M.C., Woody, R.W., (1991) Biopolymers, 31, pp. 569-586
Kelly, S.M., Price, N.C., (2000) Curr Protein Peptide Sci, 1, pp. 349-384
Mierke, D. F., Giragossian, C., (2001) Med Res Rev, 21, pp. 450-471
Aloj, L., Carac, C., Panico, M., Zannetti, A., Del Vecchio, S., Tesauro, D., De Luca, S., Salvatore, M., (2004) J Nucl Med, 45, pp. 485-494
Reubi, J. C., Waser, B., Schaer, J. C., (1998) Eur J Nucl Med, 25, pp. 481-490
Permyakov, E. A., (1993) Luminescent Spectroscopy of Proteins, , CRC Press: Boca Raton, FL
Pace, C. N., Vajdos, F., Fee, L., Grimsley, G., Gray, T., (1995) Protein Sci, 4, pp. 2411-2423
Gill, S. C., Von Hippel, P. H., (1989) Anal Biochem, 182, pp. 319-326
Emerson, M. F., Holtzer, A., (1965) J Phys Chem, 69, pp. 3718-3721
Lauterwein, J., B sch, C., Brown, L. R., W thrich, K., (1979) Biochim Biophys Acta, 556, pp. 244-264
Brown, L. R., B sch, C., W thrich, K., (1981) Biochim Biophys Acta, 642, pp. 296-312
Robinson, N. C., Tanford, C., (1975) Biochemistry, 14, pp. 369-378
Grefrath, S. P., Reynolds, J. A., (1974) Proc Natl Acad Sci U S A, 71, pp. 3913-3916
Reynolds, J. A., Stoeckenius, W., (1977) Proc Natl Acad Sci U S A, 74, pp. 2803-2804
Manning, M. C., Woody, R. W., (1991) Biopolymers, 31, pp. 569-586
Kelly, S. M., Price, N. C., (2000) Curr Protein Peptide Sci, 1, pp. 349-384
Receptor fragment approach to the binding between CCK8 peptide and cholecystokinin receptors: A fluorescence study on type B receptor fragment CCKB-R (352-379)
Fluorescence titrations in a membrane mimetic solvent system allowed us to estimate that the dissociation constant of the bimolecular complex between CCK8 peptide and cholecystokinin type B receptor fragment CCKB-R (352-379) is in the micromolar range. When considered in the context of the full receptor/ligand model, these experiments demonstrate that the receptor fragment chosen on the basis of previous structural studies represents a reliable model system to monitor the ability of CCK8 or CCK8 analogs to bind the cholecystokinin receptor. Together with previous studies, this confirms that the receptor fragment approach adopted to define the binding mode of the CCK8 fragment of cholecystokinin with its two receptors, CCKA and CCKB can be used to characterize the binding from the equilibrium standpoint. In this context, fluorescence spectros-copy proves to be the favored technique to measure dissociation constants in the nanomolar to micromolar range. (C) 2005 Wiley Periodicals, Inc.
Receptor fragment approach to the binding between CCK8 peptide and cholecystokinin receptors: A fluorescence study on type B receptor fragment CCKB-R (352-379)
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Receptor fragment approach to the binding between CCK8 peptide and cholecystokinin receptors: A fluorescence study on type B receptor fragment CCKB-R (352-379)