The magic spot ppGpp influences in vitro the molecular and functional properties of the elongation factor 1α from the archaeon Sulfolobus solfataricus (349 views)
Extremophiles (ISSN: 1431-0651, 1433-4909, 1433-4909electronic), 2012 Sep; 16(5): 743-749.
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Paper type: Journal Article,
Impact factor: 2.203, 5-year impact factor: 2.46
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84865830329&partnerID=40&md5=1610afae0c51bad27dbaceed895a77a3
Keywords: Elongation Factor 1α, Intrinsic Gtpase, Magic Spot, Ppgpp, Stringent Control, Sulfolobus Solfataricus, Aminoacyl Transfer Rna, Archaeal Protein, Archaeal Rna, Guanosine 3, Diphosphate 5, Article, Genetics, Metabolism, Physiology, Protein Synthesis, Guanosine Tetraphosphate, Peptide Elongation Factor 1, Protein Biosynthesis, Amino Acyl, Bacteria (microorganisms), Eukaryota,
Affiliations:
*** IBB - CNR ***
Dipartimento di Studi delle Istituzioni e dei Sistemi Territoriali, Università degli Studi di Napoli Parthenope, Via Medina 40, 80133 Naples, Italy
Dipartimento di Scienze Farmacobiologiche, Università degli Studi Magna Graecia di Catanzaro, Complesso Ninì Barbieri, 88021 Roccelletta di Borgia (CZ), Italy
Dipartimento di Biochimica e Biotecnologie Mediche, Università degli Studi di Napoli Federico II, Via S. Pansini 5, 80131 Naples, Italy
Istituto di Biostrutture e Bioimmagini, CNR, Via Mezzocannone 16, 80134 Naples, Italy
CEINGE Biotecnologie Avanzate s.c.a r.l, Via G. Salvatore 486, 80145 Naples, Italy
References:
Atkinson, G.C., Tenson, T., Hauryliuk, V., The RelA/SpoT homolog (RSH) superfamily: distribution and functional evolution of ppGpp synthetases and hydrolases across the tree of life (2011) PLoS One, 6, pp. e2347
Battesti, A., Bouveret, E., Acyl carrier protein/SpoT interaction, the switch linking SpoT-dependent stress response to fatty acid metabolism (2006) Mol Microbiol, 62, pp. 1048-1063
Braeken, K., Moris, M., Daniels, R., Vanderleyden, J., Michiels, J., New horizons for (p)ppGpp in bacterial and plant physiology (2006) J Trends Microbiol, 14, pp. 45-54
Cashel, M., Gentry, D.R., Hernandez, V.J., Vinella, D., The stringent response (1996) Escherichia Coli and Salmonella: Cellular and Molecular Biology, pp. 1458-1496. , 2nd edn., F. C. Neidhardt, R. CurtissIII, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. Reznicoff, M. Riley, M. Schaechter, and A. E. Umbarger (Eds.), Washington DC: ASM Press
Cellini, A., Scoarughi, G.L., Poggiali, P., Santino, I., Sessa, R., Donini, P., Cimmino, C., Stringent control in the archaeal genus Sulfolobus (2004) Res Microbiol, 155, pp. 98-104
Cimmino, C., Scoarughi, G.L., Donini, P., Stringency and relaxation among the halobacteria (1993) J Bacteriol, 175, pp. 6659-6662
de Vendittis, E., de Paola, B., Gogliettino, M.A., Adinolfi, B.S., Fiengo, A., Duvold, T., Bocchini, V., Fusidic and helvolic acid inhibition of elongation factor 2 from the archaeon Sulfolobus solfataricus (2002) Biochemistry, 41, pp. 14879-14884
Dix, D.B., Thompson, R.C., Elongation factor Tu·guanosine 3′-diphosphate 5′-diphosphate complex increases the fidelity of proofreading in protein biosynthesis: mechanism for reducing translational errors introduced by amino acid starvation (1986) Proc Natl Acad Sci USA, 83, pp. 2027-2031
Granata, V., Graziano, G., Ruggiero, A., Raimo, G., Masullo, M., Arcari, P., Vitagliano, L., Zagari, A., Chemical denaturation of the elongation factor 1alpha isolated from the hyperthermophilic archaeon Sulfolobus solfataricus (2006) Biochemistry, 45, pp. 719-726
Ianniciello, G., Masullo, M., Gallo, M., Arcari, P., Bocchini, V., Expression in Escherichia coli of thermostable elongation factor 1 alpha from the archaeon Sulfolobus solfataricus (1996) Biotechnol Appl Biochem, 23, pp. 41-45
Iglesias-Gato, D., MartÃn-Marcos, P., Santos, M.A., Hinnebusch, A.G., Tamame, M., Guanine nucleotide pool imbalance impairs multiple steps of protein synthesis and disrupts GCN4 translational control in Saccharomyces cerevisiae (2011) Genetics, 187, pp. 105-122
Kasai, K., Usami, S., Yamada, T., Endo, Y., Ochi, K., Tozawa, Y., A RelA-SpoT homolog (Cr-RSH) identified in Chlamydomonas reinhardtii generates stringent factor in vivo and localizes to chloroplasts in vitro (2002) Nucleic Acids Res, 30, pp. 4985-4992
Legault, L., Jeantet, C., Gros, F., Inhibition of in vitro protein synthesis by ppGpp (1972) FEBS Lett, 27, pp. 71-75
Lombardo, B., Raimo, G., Bocchini, V., Molecular and functional properties of an archaeal phenylalanyl-tRNA synthetase from the hyperthermophile Sulfolobus solfataricus (2002) Biochim Biophys Acta, 1596, pp. 246-252
Magnusson, L.U., Farewell, A., Nyström, T., ppGpp: a global regulator in Escherichia coli (2005) Trends Microbiol, 13, pp. 236-242
Manzocchi, L.A., Tarragó, A., Allende, J.E., The effect of ppGpp on in vitro protein synthesis by a wheat embryo system (1973) FEBS Lett, 29, pp. 309-312
Masullo, M., Raimo, G., Parente, A., Gambacorta, A., de Rosa, M., Bocchini, V., Properties of the elongation factor 1 alpha in the thermoacidophilic archaebacterium Sulfolobus solfataricus (1991) Eur J Biochem, 18, pp. 529-537
Masullo, M., de Vendittis, E., Bocchini, V., Archaebacterial elongation factor 1 alpha carries the catalytic site for GTP hydrolysis (1994) J Biol Chem, 269, pp. 20376-20379
Masullo, M., Ianniciello, G., Arcari, P., Bocchini, V., Properties of truncated forms of the elongation factor 1alpha from the archaeon Sulfolobus solfataricus (1997) Eur J Biochem, 243, pp. 468-473
Masullo, M., Cantiello, P., de Paola, B., Fiengo, A., Vitagliano, L., Zagari, A., Arcari, P., Valine 114 replacements in archaeal elongation factor 1 alpha enhanced its ability to interact with aminoacyl-tRNA and kirromycin (2002) Biochemistry, 41, pp. 14482-14488
Milon, P., Tischenko, E., Tomsic, J., Caserta, E., Folkers, G., La Teana, A., Rodnina, M.V., Gualerzi, C.O., The nucleotide-binding site of bacterial translation initiation factor 2 (IF2) as a metabolic sensor (2006) Proc Natl Acad Sci USA, 103, pp. 13962-13967
Mitkevich, V.A., Ermakov, A., Kulikova, A.A., Tankov, S., Shyp, V., Soosaar, A., Tenson, T., Hauryliuk, V., Thermodynamic characterization of ppGpp binding to EF-G or IF2 and of initiator tRNA binding to free IF2 in the presence of GDP, GTP, or ppGpp (2010) J Mol Biol, 402, pp. 838-846
Mizusawa, K., Masuda, S., Ohta, H., Expression profiling of four RelA/SpoT-like proteins, homologues of bacterial stringent factors, in Arabidopsis thaliana (2008) Planta, 228, pp. 553-562
Nomura, Y., Takabayashi, T., Kuroda, H., Yukawa, Y., Sattasuk, K., Akita, M., Nozawa, A., Tozawa, Y., ppGpp inhibits peptide elongation cycle of chloroplast translation system in vitro (2012) Plant Mol Biol, 78, pp. 185-196
Potrykus, K., Cashel, M., (p)ppGpp still magical? (2008) Annu Rev Microbiol, 62, pp. 35-51
Rojas, A.M., Ehrenberg, M., Andersson, S.G., Kurland, C.G., ppGpp inhibition of elongation factors Tu, G and Ts during polypeptide synthesis (1984) Mol Gen Genet, 197, pp. 36-45
Sun, D., Lee, G., Lee, J.H., Kim, H.Y., Rhee, H.W., Park, S.Y., Kim, K.J., Chung, J., A metazoan ortholog of SpoT hydrolyzes ppGpp and functions in starvation responses (2010) Nat Struct Mol Biol, 17, pp. 1188-1194
Takahashi, K., Kasai, K., Ochi, K., Identification of the bacterial alarmone guanosine 5′-diphosphate 3′-diphosphate (ppGpp) in plants (2004) Proc Natl Acad Sci USA, 101, pp. 4320-4324
Underfriend, S., (1969) Fluorescence Assay in Biology and Medicine, , London: Academic Press
van der Biezen, E.A., Sun, J., Coleman, M.J., Bibb, M.J., Jones, J.D., Arabidopsis RelA/SpoT homologs implicate (p)ppGpp in plant signaling (2000) Proc Natl Acad Sci USA, 28, pp. 3747-3752
Vitagliano, L., Masullo, M., Sica, F., Zagari, A., Bocchini, V., The crystal structure of Sulfolobus solfataricus elongation factor 1alpha in complex with GDP reveals novel features in nucleotide binding and exchange (2001) EMBO J, 20, pp. 5305-5311
Vitagliano, L., Ruggiero, A., Masullo, M., Cantiello, P., Arcari, P., Zagari, A., The crystal structure of Sulfolobus solfataricus elongation factor 1 alpha in complex with magnesium and GDP (2004) Biochemistry, 43, pp. 6630-6636
Yoshida, M., Travers, A., Clark, B.F., Inhibition of translation initiation complex formation by MS1 (1972) FEBS Lett, 23, pp. 163-166
Atkinson, G. C., Tenson, T., Hauryliuk, V., The RelA/SpoT homolog (RSH) superfamily: distribution and functional evolution of ppGpp synthetases and hydrolases across the tree of life (2011) PLoS One, 6, pp. e2347
Dix, D. B., Thompson, R. C., Elongation factor Tu guanosine 3 -diphosphate 5 -diphosphate complex increases the fidelity of proofreading in protein biosynthesis: mechanism for reducing translational errors introduced by amino acid starvation (1986) Proc Natl Acad Sci USA, 83, pp. 2027-2031
Magnusson, L. U., Farewell, A., Nystr m, T., ppGpp: a global regulator in Escherichia coli (2005) Trends Microbiol, 13, pp. 236-242
Manzocchi, L. A., Tarrag, A., Allende, J. E., The effect of ppGpp on in vitro protein synthesis by a wheat embryo system (1973) FEBS Lett, 29, pp. 309-312
Mitkevich, V. A., Ermakov, A., Kulikova, A. A., Tankov, S., Shyp, V., Soosaar, A., Tenson, T., Hauryliuk, V., Thermodynamic characterization of ppGpp binding to EF-G or IF2 and of initiator tRNA binding to free IF2 in the presence of GDP, GTP, or ppGpp (2010) J Mol Biol, 402, pp. 838-846
Rojas, A. M., Ehrenberg, M., Andersson, S. G., Kurland, C. G., ppGpp inhibition of elongation factors Tu, G and Ts during polypeptide synthesis (1984) Mol Gen Genet, 197, pp. 36-45
van der Biezen, E. A., Sun, J., Coleman, M. J., Bibb, M. J., Jones, J. D., Arabidopsis RelA/SpoT homologs implicate (p) ppGpp in plant signaling (2000) Proc Natl Acad Sci USA, 28, pp. 3747-3752
Extremophiles (ISSN: 1431-0651, 1433-4909, 1433-4909electronic), 2012 Sep; 16(5): 743-749.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 2.203, 5-year impact factor: 2.46
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84865830329&partnerID=40&md5=1610afae0c51bad27dbaceed895a77a3
Keywords: Elongation Factor 1α, Intrinsic Gtpase, Magic Spot, Ppgpp, Stringent Control, Sulfolobus Solfataricus, Aminoacyl Transfer Rna, Archaeal Protein, Archaeal Rna, Guanosine 3, Diphosphate 5, Article, Genetics, Metabolism, Physiology, Protein Synthesis, Guanosine Tetraphosphate, Peptide Elongation Factor 1, Protein Biosynthesis, Amino Acyl, Bacteria (microorganisms), Eukaryota,
Affiliations:
*** IBB - CNR ***
Dipartimento di Studi delle Istituzioni e dei Sistemi Territoriali, Università degli Studi di Napoli Parthenope, Via Medina 40, 80133 Naples, Italy
Dipartimento di Scienze Farmacobiologiche, Università degli Studi Magna Graecia di Catanzaro, Complesso Ninì Barbieri, 88021 Roccelletta di Borgia (CZ), Italy
Dipartimento di Biochimica e Biotecnologie Mediche, Università degli Studi di Napoli Federico II, Via S. Pansini 5, 80131 Naples, Italy
Istituto di Biostrutture e Bioimmagini, CNR, Via Mezzocannone 16, 80134 Naples, Italy
CEINGE Biotecnologie Avanzate s.c.a r.l, Via G. Salvatore 486, 80145 Naples, Italy
References:
Atkinson, G.C., Tenson, T., Hauryliuk, V., The RelA/SpoT homolog (RSH) superfamily: distribution and functional evolution of ppGpp synthetases and hydrolases across the tree of life (2011) PLoS One, 6, pp. e2347
Battesti, A., Bouveret, E., Acyl carrier protein/SpoT interaction, the switch linking SpoT-dependent stress response to fatty acid metabolism (2006) Mol Microbiol, 62, pp. 1048-1063
Braeken, K., Moris, M., Daniels, R., Vanderleyden, J., Michiels, J., New horizons for (p)ppGpp in bacterial and plant physiology (2006) J Trends Microbiol, 14, pp. 45-54
Cashel, M., Gentry, D.R., Hernandez, V.J., Vinella, D., The stringent response (1996) Escherichia Coli and Salmonella: Cellular and Molecular Biology, pp. 1458-1496. , 2nd edn., F. C. Neidhardt, R. CurtissIII, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. Reznicoff, M. Riley, M. Schaechter, and A. E. Umbarger (Eds.), Washington DC: ASM Press
Cellini, A., Scoarughi, G.L., Poggiali, P., Santino, I., Sessa, R., Donini, P., Cimmino, C., Stringent control in the archaeal genus Sulfolobus (2004) Res Microbiol, 155, pp. 98-104
Cimmino, C., Scoarughi, G.L., Donini, P., Stringency and relaxation among the halobacteria (1993) J Bacteriol, 175, pp. 6659-6662
de Vendittis, E., de Paola, B., Gogliettino, M.A., Adinolfi, B.S., Fiengo, A., Duvold, T., Bocchini, V., Fusidic and helvolic acid inhibition of elongation factor 2 from the archaeon Sulfolobus solfataricus (2002) Biochemistry, 41, pp. 14879-14884
Dix, D.B., Thompson, R.C., Elongation factor Tu·guanosine 3′-diphosphate 5′-diphosphate complex increases the fidelity of proofreading in protein biosynthesis: mechanism for reducing translational errors introduced by amino acid starvation (1986) Proc Natl Acad Sci USA, 83, pp. 2027-2031
Granata, V., Graziano, G., Ruggiero, A., Raimo, G., Masullo, M., Arcari, P., Vitagliano, L., Zagari, A., Chemical denaturation of the elongation factor 1alpha isolated from the hyperthermophilic archaeon Sulfolobus solfataricus (2006) Biochemistry, 45, pp. 719-726
Ianniciello, G., Masullo, M., Gallo, M., Arcari, P., Bocchini, V., Expression in Escherichia coli of thermostable elongation factor 1 alpha from the archaeon Sulfolobus solfataricus (1996) Biotechnol Appl Biochem, 23, pp. 41-45
Iglesias-Gato, D., MartÃn-Marcos, P., Santos, M.A., Hinnebusch, A.G., Tamame, M., Guanine nucleotide pool imbalance impairs multiple steps of protein synthesis and disrupts GCN4 translational control in Saccharomyces cerevisiae (2011) Genetics, 187, pp. 105-122
Kasai, K., Usami, S., Yamada, T., Endo, Y., Ochi, K., Tozawa, Y., A RelA-SpoT homolog (Cr-RSH) identified in Chlamydomonas reinhardtii generates stringent factor in vivo and localizes to chloroplasts in vitro (2002) Nucleic Acids Res, 30, pp. 4985-4992
Legault, L., Jeantet, C., Gros, F., Inhibition of in vitro protein synthesis by ppGpp (1972) FEBS Lett, 27, pp. 71-75
Lombardo, B., Raimo, G., Bocchini, V., Molecular and functional properties of an archaeal phenylalanyl-tRNA synthetase from the hyperthermophile Sulfolobus solfataricus (2002) Biochim Biophys Acta, 1596, pp. 246-252
Magnusson, L.U., Farewell, A., Nyström, T., ppGpp: a global regulator in Escherichia coli (2005) Trends Microbiol, 13, pp. 236-242
Manzocchi, L.A., Tarragó, A., Allende, J.E., The effect of ppGpp on in vitro protein synthesis by a wheat embryo system (1973) FEBS Lett, 29, pp. 309-312
Masullo, M., Raimo, G., Parente, A., Gambacorta, A., de Rosa, M., Bocchini, V., Properties of the elongation factor 1 alpha in the thermoacidophilic archaebacterium Sulfolobus solfataricus (1991) Eur J Biochem, 18, pp. 529-537
Masullo, M., de Vendittis, E., Bocchini, V., Archaebacterial elongation factor 1 alpha carries the catalytic site for GTP hydrolysis (1994) J Biol Chem, 269, pp. 20376-20379
Masullo, M., Ianniciello, G., Arcari, P., Bocchini, V., Properties of truncated forms of the elongation factor 1alpha from the archaeon Sulfolobus solfataricus (1997) Eur J Biochem, 243, pp. 468-473
Masullo, M., Cantiello, P., de Paola, B., Fiengo, A., Vitagliano, L., Zagari, A., Arcari, P., Valine 114 replacements in archaeal elongation factor 1 alpha enhanced its ability to interact with aminoacyl-tRNA and kirromycin (2002) Biochemistry, 41, pp. 14482-14488
Milon, P., Tischenko, E., Tomsic, J., Caserta, E., Folkers, G., La Teana, A., Rodnina, M.V., Gualerzi, C.O., The nucleotide-binding site of bacterial translation initiation factor 2 (IF2) as a metabolic sensor (2006) Proc Natl Acad Sci USA, 103, pp. 13962-13967
Mitkevich, V.A., Ermakov, A., Kulikova, A.A., Tankov, S., Shyp, V., Soosaar, A., Tenson, T., Hauryliuk, V., Thermodynamic characterization of ppGpp binding to EF-G or IF2 and of initiator tRNA binding to free IF2 in the presence of GDP, GTP, or ppGpp (2010) J Mol Biol, 402, pp. 838-846
Mizusawa, K., Masuda, S., Ohta, H., Expression profiling of four RelA/SpoT-like proteins, homologues of bacterial stringent factors, in Arabidopsis thaliana (2008) Planta, 228, pp. 553-562
Nomura, Y., Takabayashi, T., Kuroda, H., Yukawa, Y., Sattasuk, K., Akita, M., Nozawa, A., Tozawa, Y., ppGpp inhibits peptide elongation cycle of chloroplast translation system in vitro (2012) Plant Mol Biol, 78, pp. 185-196
Potrykus, K., Cashel, M., (p)ppGpp still magical? (2008) Annu Rev Microbiol, 62, pp. 35-51
Rojas, A.M., Ehrenberg, M., Andersson, S.G., Kurland, C.G., ppGpp inhibition of elongation factors Tu, G and Ts during polypeptide synthesis (1984) Mol Gen Genet, 197, pp. 36-45
Sun, D., Lee, G., Lee, J.H., Kim, H.Y., Rhee, H.W., Park, S.Y., Kim, K.J., Chung, J., A metazoan ortholog of SpoT hydrolyzes ppGpp and functions in starvation responses (2010) Nat Struct Mol Biol, 17, pp. 1188-1194
Takahashi, K., Kasai, K., Ochi, K., Identification of the bacterial alarmone guanosine 5′-diphosphate 3′-diphosphate (ppGpp) in plants (2004) Proc Natl Acad Sci USA, 101, pp. 4320-4324
Underfriend, S., (1969) Fluorescence Assay in Biology and Medicine, , London: Academic Press
van der Biezen, E.A., Sun, J., Coleman, M.J., Bibb, M.J., Jones, J.D., Arabidopsis RelA/SpoT homologs implicate (p)ppGpp in plant signaling (2000) Proc Natl Acad Sci USA, 28, pp. 3747-3752
Vitagliano, L., Masullo, M., Sica, F., Zagari, A., Bocchini, V., The crystal structure of Sulfolobus solfataricus elongation factor 1alpha in complex with GDP reveals novel features in nucleotide binding and exchange (2001) EMBO J, 20, pp. 5305-5311
Vitagliano, L., Ruggiero, A., Masullo, M., Cantiello, P., Arcari, P., Zagari, A., The crystal structure of Sulfolobus solfataricus elongation factor 1 alpha in complex with magnesium and GDP (2004) Biochemistry, 43, pp. 6630-6636
Yoshida, M., Travers, A., Clark, B.F., Inhibition of translation initiation complex formation by MS1 (1972) FEBS Lett, 23, pp. 163-166
Atkinson, G. C., Tenson, T., Hauryliuk, V., The RelA/SpoT homolog (RSH) superfamily: distribution and functional evolution of ppGpp synthetases and hydrolases across the tree of life (2011) PLoS One, 6, pp. e2347
Dix, D. B., Thompson, R. C., Elongation factor Tu guanosine 3 -diphosphate 5 -diphosphate complex increases the fidelity of proofreading in protein biosynthesis: mechanism for reducing translational errors introduced by amino acid starvation (1986) Proc Natl Acad Sci USA, 83, pp. 2027-2031
Magnusson, L. U., Farewell, A., Nystr m, T., ppGpp: a global regulator in Escherichia coli (2005) Trends Microbiol, 13, pp. 236-242
Manzocchi, L. A., Tarrag, A., Allende, J. E., The effect of ppGpp on in vitro protein synthesis by a wheat embryo system (1973) FEBS Lett, 29, pp. 309-312
Mitkevich, V. A., Ermakov, A., Kulikova, A. A., Tankov, S., Shyp, V., Soosaar, A., Tenson, T., Hauryliuk, V., Thermodynamic characterization of ppGpp binding to EF-G or IF2 and of initiator tRNA binding to free IF2 in the presence of GDP, GTP, or ppGpp (2010) J Mol Biol, 402, pp. 838-846
Rojas, A. M., Ehrenberg, M., Andersson, S. G., Kurland, C. G., ppGpp inhibition of elongation factors Tu, G and Ts during polypeptide synthesis (1984) Mol Gen Genet, 197, pp. 36-45
van der Biezen, E. A., Sun, J., Coleman, M. J., Bibb, M. J., Jones, J. D., Arabidopsis RelA/SpoT homologs implicate (p) ppGpp in plant signaling (2000) Proc Natl Acad Sci USA, 28, pp. 3747-3752
The magic spot ppGpp influences in vitro the molecular and functional properties of the elongation factor 1α from the archaeon Sulfolobus solfataricus
Guanosine tetra-phosphate (ppGpp), also known as "magic spot I", is a key molecule in the stringent control of most eubacteria and some eukarya. Here, we show that ppGpp affects the functional and molecular properties of the archaeal elongation factor 1α from Sulfolobus solfataricus (SsEF-1α). Indeed, ppGpp inhibited archaeal protein synthesis in vitro, even though the concentration required to get inhibition was higher than that required for the eubacterial and eukaryal systems. Regarding the partial reactions catalysed by SsEF-1α the effect produced by ppGpp on the affinity for aa-tRNA was lower than that measured in the presence of GTP but higher than that for GDP. Magic spot I was also able to bind SsEF-1α with an intermediate affinity in comparison to that displayed by GDP and GTP. Furthermore, ppGpp inhibited the intrinsic GTPase of SsEF-1α with a competitive behaviour. Finally, the binding of ppGpp to SsEF-1α rendered the elongation factor more resistant to heat treatment and the analysis of the molecular model of the complex between SsEF-1α and ppGpp suggests that this stabilisation arises from the charge optimisation on the surface of the protein. © 2012 Springer.
Guanosine tetra-phosphate (ppGpp), also known as "magic spot I", is a key molecule in the stringent control of most eubacteria and some eukarya. Here, we show that ppGpp affects the functional and molecular properties of the archaeal elongation factor 1α from Sulfolobus solfataricus (SsEF-1α). Indeed, ppGpp inhibited archaeal protein synthesis in vitro, even though the concentration required to get inhibition was higher than that required for the eubacterial and eukaryal systems. Regarding the partial reactions catalysed by SsEF-1α the effect produced by ppGpp on the affinity for aa-tRNA was lower than that measured in the presence of GTP but higher than that for GDP. Magic spot I was also able to bind SsEF-1α with an intermediate affinity in comparison to that displayed by GDP and GTP. Furthermore, ppGpp inhibited the intrinsic GTPase of SsEF-1α with a competitive behaviour. Finally, the binding of ppGpp to SsEF-1α rendered the elongation factor more resistant to heat treatment and the analysis of the molecular model of the complex between SsEF-1α and ppGpp suggests that this stabilisation arises from the charge optimisation on the surface of the protein. © 2012 Springer.
The magic spot ppGpp influences in vitro the molecular and functional properties of the elongation factor 1α from the archaeon Sulfolobus solfataricus
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The magic spot ppGpp influences in vitro the molecular and functional properties of the elongation factor 1α from the archaeon Sulfolobus solfataricus
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Palmieri G, Langella E, Gogliettino M, Saviano M, Pocsfalvi G, Rossi M
* A novel class of protease targets of phosphatidylethanolamine-binding proteins (PEBP): A study of the acylpeptide hydrolase and the PEBP inhibitor from the archaeon Sulfolobus solfataricus (376 views)
Mol Biosyst (ISSN: 1742-206x), 2010; 6(12): 2498-2507.
Impact Factor: 3.825
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Ruggiero A, Masullo M, Marasco D, Ruocco MR, Grimaldi P, Arcari P, Zagari A, Vitagliano L
* The dimeric structure of Sulfolobus solfataricus thioredoxin A2 and the basis of its thermostability (316 views)
Proteins (ISSN: 0887-3585, 1097-0134, 1097-0134electronic), 2009 Dec; 77(4): 1004-1008.
Impact Factor: 3.085
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D'Ambrosio K, Limauro D, Pedone E, Galdi I, Pedone C, Bartolucci S, De Simone G
* Insights into the catalytic mechanism of the Bcp family: functional and structural analysis of Bcp1 from Sulfolobus solfataricus (469 views)
Proteins (ISSN: 1097-0134, 0887-3585, 1097-0134electronic), 2009 Sep; 76(4): 995-1006.
Impact Factor: 3.085
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Pennacchio A, Esposito L, Zagari A, Rossi M, Raia CA
* Role of Tryptophan 95 in substrate specificity and structural stability of Sulfolobus solfataricus alcohol dehydrogenase (298 views)
Extremophiles (ISSN: 1431-0651, 1433-4909, 1433-4909electronic), 2009 Sep; 13(5): 751-761.
Impact Factor: 2
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Ruggiero I, Cantiello P, Lamberti A, Sorrentino A, Martucci NM, Ruggiero A, Arcone R, Vitagliano L, Arcari P, Masullo M
* Biochemical Characterisation Of The D60a Mutant Of The Elongation Factor 1 Alpha From The Archaeon Sulfolobus Solfataricus (307 views)
Biochimie (ISSN: 0300-9084, 1638-6183, 1638-6183electronic), 2009 Jul; 91(7): 835-842.
Impact Factor: 3.897
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Ruggiero A, Lanzotti MA, Ruocco MR, Grimaldi P, Marasco D, Arcari P, Masullo M, Zagari A, Vitagliano L
* Crystallization and preliminary X-ray crystallographic analysis of two dimeric hyperthermostable thioredoxins isolated from Sulfolobus solfataricus (337 views)
Nucleosides Nucleotides Nucleic Acids (ISSN: 1744-3091, 1744-4309), 2009 Jun; 65: 604-607.
Impact Factor: 0.551
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Di Fiore A, Fiorentino G, Vitale RM, Ronca R, Amodeo P, Pedone C, Bartolucci S, De Simone G
* Structural analysis of BldR from Sulfolobus solfataricus provides insights into the molecular basis of transcriptional activation in Archaea by MarR family proteins (470 views)
J Mol Biol (ISSN: 1089-8638, 0022-2836, 1089-8638electronic), 2009 May 8; 388(3): 559-569.
Impact Factor: 3.871
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Ruggiero A, Masullo M, Ruocco MR, Grimaldi P, Lanzotti MA, Arcari P, Zagari A, Vitagliano L
* Structure and stability of a thioredoxin reductase from Sulfolobus solfataricus: A thermostable protein with two functions (347 views)
Bba-Gen Subjects (ISSN: 1570-9639, 0006-3002, 0925-4439), 2009 Mar; 1794(3): 554-562.
Impact Factor: 2.48
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Palmieri G, Catara G, Saviano M, Langella E, Gogliettino M, Rossi M
* First archaeal PEPB-serine protease inhibitor from sulfolobus solfataricus with noncanonical amino acid sequence in the reactive-site loop (396 views)
J Proteome Res (ISSN: 1535-3893), 2009 Jan; 8(1): 327-334.
Impact Factor: 5.132
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Limauro D, Saviano M, Galdi I, Rossi M, Bartolucci S, Pedone E
* Sulfolobus solfataricus protein disulphide oxidoreductase: insight into the roles of its redox sites (234 views)
Protein Engineering Design & Selection (ISSN: 1741-0126), 2009 Jan; 22(1): 19-26.
Impact Factor: 2.596
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Grimaldi P, Ruocco MR, Lanzotti MA, Ruggiero A, Ruggiero I, Arcari P, Vitagliano L, Masullo M
* Characterisation of the components of the thioredoxin system in the archaeon Sulfolobus solfataricus (356 views)
Extremophiles (ISSN: 1431-0651, 1433-4909, 1433-4909electronic), 2008 Jul; 12(4): 553-562.
Impact Factor: 1.782
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Limauro D, Pedone E, Galdi I, Bartolucci S
* Peroxiredoxins as cellular guardians in Sulfolobus solfataricus - characterization of Bcp1, Bcp3 and Bcp4 (366 views)
Febs Journal (ISSN: 1742-464x), 2008 May; 275(9): 2067-2077.
Impact Factor: 3.139
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Granata V, Graziano G, Ruggiero A, Raimo G, Masullo M, Arcari P, Vitagliano L, Zagari A
* Stability Against Temperature Of Sulfolobus Solfataricus Elongation Factor 1 Alpha, A Multi-Domain Protein (372 views)
Bba-Gen Subjects (ISSN: 1570-9639, 0006-3002, 0925-4439), 2008 Apr; 1784(4): 573-581.
Impact Factor: 2.233
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Prato S, Vitale RM, Contursi P, Lipps G, Saviano M, Rossi M, Bartolucci S
* Molecular modeling and functional characterization of the monomeric primase-polymerase domain from the Sulfolobus solfataricus plasmid pIT3 (398 views)
Febs J (ISSN: 1742-464x), 2008; 275(17): 4389-4402.
Impact Factor: 3.139
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Pedone E, Limauro D, D'Alterio R, Rossi M, Bartolucci S
* Characterization of a multifunctional protein disulfide oxidoreductase from Sulfolobus solfataricus (328 views)
Febs Journal (ISSN: 1742-464x), 2006 Dec; 273(23): 5407-5420.
Impact Factor: 3.033
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Limauro D, Pedone E, Pirone L, Bartolucci S
* Identification and characterization of 1-Cys peroxiredoxin from Sulfolobus solfataricus and its involvement in the response to oxidative stress (362 views)
Febs Journal (ISSN: 1742-464x), 2006 Feb; 273(4): 721-731.
Impact Factor: 3.033
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Granata V, Graziano G, Ruggiero A, Raimo G, Masullo M, Arcari P, Vitagliano L, Zagari A
* Chemical Denaturation Of The Elongation Factor 1 Alpha Isolated From The Hyperthermophilic Archaeon Sulfolobus Solfataricus (343 views)
Biochemistry (ISSN: 0006-2960, 1520-4995, 1520-4995electronic), 2006 Jan 24; 45(3): 719-726.
Impact Factor: 3.633
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Ruggiero A, Masullo M, Arcari P, Raimo G, Vitagliano L, Zagari A
* Crystallization And Preliminary X-Ray Crystallographic Analysis Of The Sulfolobus Solfataricus Nucleotide-Exchange Factor 1 Beta (340 views)
Nucleosides Nucleotides Nucleic Acids (ISSN: 1744-3091, 1744-4309), 2005 Nov; 61(11): 1000-1002.
Impact Factor: 0.568
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Ruggiero A, Ruocco MR, Grimaldi P, Arcari P, Masullo M, Zagari A, Vitagliano L
* Crystallization and preliminary X-ray crystallographic analysis of Sulfolobus solfataricus thioredoxin reductase (319 views)
Nucleosides Nucleotides Nucleic Acids (ISSN: 1744-3091, 1744-4309), 2005 Oct; 61: 906-909.
Impact Factor: 0.568
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Merlino A, Graziano G, Mazzarella L
* Structural And Dynamic Effects Of Alpha-Helix Deletion In Sso7d: Implications For Protein Thermal Stability (375 views)
Proteins (ISSN: 0887-3585, 1097-0134, 1097-0134electronic), 2004 Dec 1; 57(4): 692-701.
Impact Factor: 4.429
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Vitagliano L, Ruggiero A, Masullo M, Cantiello P, Arcari P, Zagari A
* The Crystal Structure Of Sulfolobus Solfataricus Elongation Factor 1 Alpha In Complex With Magnesium And Gdp (478 views)
Biochemistry (ISSN: 0006-2960, 1520-4995, 1520-4995electronic), 2004; 43(21): 6630-6636.
Impact Factor: 4.008
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Esposito L, Bruno I, Sica F, Raia CA, Giordano A, Rossi M, Mazzarella L, Zagari A
* Crystal structure of a ternary complex of the alcohol dehydrogenase from Sulfolobus solfataricus (529 views)
Biochemistry (ISSN: 0006-2960, 1520-4995, 1520-4995electronic), 2003 Dec 16; 42(49): 14397-14407.
Impact Factor: 3.922
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Esposito L, Bruno I, Sica F, Raia CA, Giordano A, Rossi M, Mazzarella L, Zagari A
* Structural study of a single-point mutant of Sulfolobus solfataricus alcohol dehydrogenase with enhanced activity (313 views)
Febs Lett (ISSN: 0014-5793, 0014-5793print, 1873-3468electronic), 2003 Mar 27; 539(1-3): 14-18.
Impact Factor: 3.609
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Esposito L, Sica F, Raia CA, Giordano A, Rossi M, Mazzarella L, Zagari A
* Crystal Structure Of The Alcohol Dehydrogenase From The Hyperthermophilic Archaeon Sulfolobus Solfataricus At 1. 85 Angstrom Resolution (435 views)
J Mol Biol (ISSN: 0022-2836, 1089-8638, 1089-8638electronic), 2002 Apr 26; 318(2): 463-477.
Impact Factor: 5.359
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Masullo M, Cantiello P, Paola B, Fiengo A, Vitagliano L, Zagari A, Arcari P
* Valine114 Replacements In The Archaeal Elongation Factor 1a Enhanced Its Ability To Interact With Aminoacyl-Trna And Kirromycin (352 views)
Biochemistry (ISSN: 0006-2960, 1520-4995, 1520-4995electronic), 2002; 41(49): 14482-14488.
Impact Factor: 4.064
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Vitagliano L, Masullo M, Sica F, Zagari A, Bocchini V
* The crystal structure of Sulfolobus solfataricus elongation factor 1α in complex with GDP reveals novel features in nucleotide binding and exchange (452 views)
Embo J (ISSN: 0261-4189, 0261-4189linking), 2001 Oct 1; 20(19): 5305-5311.
Impact Factor: 12.459
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 Esposito L, Sica F, Sorrentino G, Berisio R, Carotenuto L, Giordano A, Raia C, Rossi M, Lamzin VS, Willson KS, Zagari A
Protein Crystal Growth In The Advance Protein Crystallisation Facility On The Lms Mission: A Comparison Of Sulfolobus Solfataricus Alchool Dehydrogenase Crystals Grown On The Ground And In Microgravity (253 views)
Acta Crystallogr D Biol Crystallogr, 1998; 54: 386-390.
Impact Factor: 0
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Esposito L, Sica F, Sorrentino G, Berisio R, Carotenuto L, Giordano A, Raia CA, Rossi M, Lamzin VS, Wilson KS, Zagari A
Protein crystal growth in the advanced protein crystallization facility on the LMS mission: A comparison of Sulfolobus solfataricus alcohol dehydrogenase crystals grown on the ground and in microgravity (433 views)
Acta Crystallogr D Biol Crystallogr (ISSN: 0907-4449, 0907-4449linking), 1998; 54(3): 386-390.
Impact Factor: 2.244
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PEARL L, DEMASI D, HEMMINGS A, SICA F, MAZZARELLA L, RAIA C, DAURIA S, ROSSI M
CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF AN NAD+-DEPENDENT ALCOHOL-DEHYDROGENASE FROM THE EXTREME THERMOPHILIC ARCHAEBACTERIUM SULFOLOBUS-SOLFATARICUS (444 views)
J Mol Biol (ISSN: 0022-2836, 1089-8638, 1089-8638electronic), 1993 Feb 5; 229(3): 782-784.
Impact Factor: 5.673
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* Ultra-rapid glutathionylation of chymotrypsinogen in its molten globule-like conformation: A comparison to archaeal proteins (62 views)
Sci Rep (ISSN: 2045-2322linking, 2045-2322electronic), 2020 Jun 2; 10(1): 8943-8943.
Impact Factor: 3.998
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Ruggiero A, Smaldone G, Esposito L, Balasco N, Vitagliano L
* Loop size optimization induces a strong thermal stabilization of the thioredoxin fold (193 views)
Febs J (ISSN: 1742-464xlinking), 2019 May; 286(9): 1752-1764.
Impact Factor: 3.129
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Pedone E, Fiorentino G, Pirone L, Contursi P, Bartolucci S, Limauro D
* Functional and structural characterization of protein disulfide oxidoreductase from Thermus thermophilus HB27 (343 views)
Extremophiles (ISSN: 1431-0651, 1433-4909, 1433-4909electronic), 2014 Jul; 18(4): 723-731.
Impact Factor: 2.306
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Limauro D, De Simone G, Pirone L, Bartolucci S, D'Ambrosio K, Pedone E
* Sulfolobus solfataricus thiol redox puzzle: characterization of an atypical protein disulfide oxidoreductase (429 views)
Extremophiles (ISSN: 1431-0651, 1433-4909, 1433-4909electronic), 2014 Mar; 18(2): 219-228.
Impact Factor: 2.306
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Esposito L, Ruggiero A, Masullo M, Ruocco MR, Lamberti A, Arcari P, Zagari A, Vitagliano L
* Crystallographic and spectroscopic characterizations of Sulfolobus solfataricus TrxA1 provide insights into the determinants of thioredoxin fold stability (300 views)
J Struct Biol (ISSN: 1047-8477, 1047-8477linking), 2012 Feb; 177(2): 506-512.
Impact Factor: 3.361
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Palmieri G, Bergamo P, Luini A, Ruvo M, Gogliettino M, Langella E, Saviano M, Hegde RN, Sandomenico A, Rossi M
* Acylpeptide hydrolase inhibition as targeted strategy to induce proteasomal down-regulation (490 views)
Plosone (ISSN: 1932-6203, 1932-6203electronic, 1932-6203linking), 2011 Oct 10; 6(10): e25888-e25888.
Impact Factor: 4.092
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Limauro D, D'Ambrosio K, Langella E, De Simone G, Galdi I, Pedone C, Pedone E, Bartolucci S
* Exploring the catalytic mechanism of the first dimeric Bcp: Functional, structural and docking analyses of Bcp4 from Sulfolobus solfataricus (373 views)
Biochimie (ISSN: 1638-6183, 0300-9084, 1638-6183electronic), 2010 Oct; 92(10): 1435-1444.
Impact Factor: 3.787
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Palmieri G, Langella E, Gogliettino M, Saviano M, Pocsfalvi G, Rossi M
* A novel class of protease targets of phosphatidylethanolamine-binding proteins (PEBP): A study of the acylpeptide hydrolase and the PEBP inhibitor from the archaeon Sulfolobus solfataricus (376 views)
Mol Biosyst (ISSN: 1742-206x), 2010; 6(12): 2498-2507.
Impact Factor: 3.825
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Ruggiero A, Masullo M, Marasco D, Ruocco MR, Grimaldi P, Arcari P, Zagari A, Vitagliano L
* The dimeric structure of Sulfolobus solfataricus thioredoxin A2 and the basis of its thermostability (316 views)
Proteins (ISSN: 0887-3585, 1097-0134, 1097-0134electronic), 2009 Dec; 77(4): 1004-1008.
Impact Factor: 3.085
View Export to BibTeX Export to EndNote
D'Ambrosio K, Limauro D, Pedone E, Galdi I, Pedone C, Bartolucci S, De Simone G
* Insights into the catalytic mechanism of the Bcp family: functional and structural analysis of Bcp1 from Sulfolobus solfataricus (469 views)
Proteins (ISSN: 1097-0134, 0887-3585, 1097-0134electronic), 2009 Sep; 76(4): 995-1006.
Impact Factor: 3.085
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Pennacchio A, Esposito L, Zagari A, Rossi M, Raia CA
* Role of Tryptophan 95 in substrate specificity and structural stability of Sulfolobus solfataricus alcohol dehydrogenase (298 views)
Extremophiles (ISSN: 1431-0651, 1433-4909, 1433-4909electronic), 2009 Sep; 13(5): 751-761.
Impact Factor: 2
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Ruggiero I, Cantiello P, Lamberti A, Sorrentino A, Martucci NM, Ruggiero A, Arcone R, Vitagliano L, Arcari P, Masullo M
* Biochemical Characterisation Of The D60a Mutant Of The Elongation Factor 1 Alpha From The Archaeon Sulfolobus Solfataricus (307 views)
Biochimie (ISSN: 0300-9084, 1638-6183, 1638-6183electronic), 2009 Jul; 91(7): 835-842.
Impact Factor: 3.897
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Ruggiero A, Lanzotti MA, Ruocco MR, Grimaldi P, Marasco D, Arcari P, Masullo M, Zagari A, Vitagliano L
* Crystallization and preliminary X-ray crystallographic analysis of two dimeric hyperthermostable thioredoxins isolated from Sulfolobus solfataricus (337 views)
Nucleosides Nucleotides Nucleic Acids (ISSN: 1744-3091, 1744-4309), 2009 Jun; 65: 604-607.
Impact Factor: 0.551
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Di Fiore A, Fiorentino G, Vitale RM, Ronca R, Amodeo P, Pedone C, Bartolucci S, De Simone G
* Structural analysis of BldR from Sulfolobus solfataricus provides insights into the molecular basis of transcriptional activation in Archaea by MarR family proteins (470 views)
J Mol Biol (ISSN: 1089-8638, 0022-2836, 1089-8638electronic), 2009 May 8; 388(3): 559-569.
Impact Factor: 3.871
View Export to BibTeX Export to EndNote
Ruggiero A, Masullo M, Ruocco MR, Grimaldi P, Lanzotti MA, Arcari P, Zagari A, Vitagliano L
* Structure and stability of a thioredoxin reductase from Sulfolobus solfataricus: A thermostable protein with two functions (347 views)
Bba-Gen Subjects (ISSN: 1570-9639, 0006-3002, 0925-4439), 2009 Mar; 1794(3): 554-562.
Impact Factor: 2.48
View Export to BibTeX Export to EndNote
Palmieri G, Catara G, Saviano M, Langella E, Gogliettino M, Rossi M
* First archaeal PEPB-serine protease inhibitor from sulfolobus solfataricus with noncanonical amino acid sequence in the reactive-site loop (396 views)
J Proteome Res (ISSN: 1535-3893), 2009 Jan; 8(1): 327-334.
Impact Factor: 5.132
View Export to BibTeX Export to EndNote
Limauro D, Saviano M, Galdi I, Rossi M, Bartolucci S, Pedone E
* Sulfolobus solfataricus protein disulphide oxidoreductase: insight into the roles of its redox sites (234 views)
Protein Engineering Design & Selection (ISSN: 1741-0126), 2009 Jan; 22(1): 19-26.
Impact Factor: 2.596
View Export to BibTeX Export to EndNote
Grimaldi P, Ruocco MR, Lanzotti MA, Ruggiero A, Ruggiero I, Arcari P, Vitagliano L, Masullo M
* Characterisation of the components of the thioredoxin system in the archaeon Sulfolobus solfataricus (356 views)
Extremophiles (ISSN: 1431-0651, 1433-4909, 1433-4909electronic), 2008 Jul; 12(4): 553-562.
Impact Factor: 1.782
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Limauro D, Pedone E, Galdi I, Bartolucci S
* Peroxiredoxins as cellular guardians in Sulfolobus solfataricus - characterization of Bcp1, Bcp3 and Bcp4 (366 views)
Febs Journal (ISSN: 1742-464x), 2008 May; 275(9): 2067-2077.
Impact Factor: 3.139
View Export to BibTeX Export to EndNote
Granata V, Graziano G, Ruggiero A, Raimo G, Masullo M, Arcari P, Vitagliano L, Zagari A
* Stability Against Temperature Of Sulfolobus Solfataricus Elongation Factor 1 Alpha, A Multi-Domain Protein (372 views)
Bba-Gen Subjects (ISSN: 1570-9639, 0006-3002, 0925-4439), 2008 Apr; 1784(4): 573-581.
Impact Factor: 2.233
View Export to BibTeX Export to EndNote
Prato S, Vitale RM, Contursi P, Lipps G, Saviano M, Rossi M, Bartolucci S
* Molecular modeling and functional characterization of the monomeric primase-polymerase domain from the Sulfolobus solfataricus plasmid pIT3 (398 views)
Febs J (ISSN: 1742-464x), 2008; 275(17): 4389-4402.
Impact Factor: 3.139
View Export to BibTeX Export to EndNote
Pedone E, Limauro D, D'Alterio R, Rossi M, Bartolucci S
* Characterization of a multifunctional protein disulfide oxidoreductase from Sulfolobus solfataricus (328 views)
Febs Journal (ISSN: 1742-464x), 2006 Dec; 273(23): 5407-5420.
Impact Factor: 3.033
View Export to BibTeX Export to EndNote
Limauro D, Pedone E, Pirone L, Bartolucci S
* Identification and characterization of 1-Cys peroxiredoxin from Sulfolobus solfataricus and its involvement in the response to oxidative stress (362 views)
Febs Journal (ISSN: 1742-464x), 2006 Feb; 273(4): 721-731.
Impact Factor: 3.033
View Export to BibTeX Export to EndNote
Granata V, Graziano G, Ruggiero A, Raimo G, Masullo M, Arcari P, Vitagliano L, Zagari A
* Chemical Denaturation Of The Elongation Factor 1 Alpha Isolated From The Hyperthermophilic Archaeon Sulfolobus Solfataricus (343 views)
Biochemistry (ISSN: 0006-2960, 1520-4995, 1520-4995electronic), 2006 Jan 24; 45(3): 719-726.
Impact Factor: 3.633
View Export to BibTeX Export to EndNote
Ruggiero A, Masullo M, Arcari P, Raimo G, Vitagliano L, Zagari A
* Crystallization And Preliminary X-Ray Crystallographic Analysis Of The Sulfolobus Solfataricus Nucleotide-Exchange Factor 1 Beta (340 views)
Nucleosides Nucleotides Nucleic Acids (ISSN: 1744-3091, 1744-4309), 2005 Nov; 61(11): 1000-1002.
Impact Factor: 0.568
View Export to BibTeX Export to EndNote
Ruggiero A, Ruocco MR, Grimaldi P, Arcari P, Masullo M, Zagari A, Vitagliano L
* Crystallization and preliminary X-ray crystallographic analysis of Sulfolobus solfataricus thioredoxin reductase (319 views)
Nucleosides Nucleotides Nucleic Acids (ISSN: 1744-3091, 1744-4309), 2005 Oct; 61: 906-909.
Impact Factor: 0.568
View Export to BibTeX Export to EndNote
Merlino A, Graziano G, Mazzarella L
* Structural And Dynamic Effects Of Alpha-Helix Deletion In Sso7d: Implications For Protein Thermal Stability (375 views)
Proteins (ISSN: 0887-3585, 1097-0134, 1097-0134electronic), 2004 Dec 1; 57(4): 692-701.
Impact Factor: 4.429
View Export to BibTeX Export to EndNote
Vitagliano L, Ruggiero A, Masullo M, Cantiello P, Arcari P, Zagari A
* The Crystal Structure Of Sulfolobus Solfataricus Elongation Factor 1 Alpha In Complex With Magnesium And Gdp (478 views)
Biochemistry (ISSN: 0006-2960, 1520-4995, 1520-4995electronic), 2004; 43(21): 6630-6636.
Impact Factor: 4.008
View Export to BibTeX Export to EndNote
Esposito L, Bruno I, Sica F, Raia CA, Giordano A, Rossi M, Mazzarella L, Zagari A
* Crystal structure of a ternary complex of the alcohol dehydrogenase from Sulfolobus solfataricus (529 views)
Biochemistry (ISSN: 0006-2960, 1520-4995, 1520-4995electronic), 2003 Dec 16; 42(49): 14397-14407.
Impact Factor: 3.922
View Export to BibTeX Export to EndNote
Esposito L, Bruno I, Sica F, Raia CA, Giordano A, Rossi M, Mazzarella L, Zagari A
* Structural study of a single-point mutant of Sulfolobus solfataricus alcohol dehydrogenase with enhanced activity (313 views)
Febs Lett (ISSN: 0014-5793, 0014-5793print, 1873-3468electronic), 2003 Mar 27; 539(1-3): 14-18.
Impact Factor: 3.609
View Export to BibTeX Export to EndNote
Esposito L, Sica F, Raia CA, Giordano A, Rossi M, Mazzarella L, Zagari A
* Crystal Structure Of The Alcohol Dehydrogenase From The Hyperthermophilic Archaeon Sulfolobus Solfataricus At 1. 85 Angstrom Resolution (435 views)
J Mol Biol (ISSN: 0022-2836, 1089-8638, 1089-8638electronic), 2002 Apr 26; 318(2): 463-477.
Impact Factor: 5.359
View Export to BibTeX Export to EndNote
Masullo M, Cantiello P, Paola B, Fiengo A, Vitagliano L, Zagari A, Arcari P
* Valine114 Replacements In The Archaeal Elongation Factor 1a Enhanced Its Ability To Interact With Aminoacyl-Trna And Kirromycin (352 views)
Biochemistry (ISSN: 0006-2960, 1520-4995, 1520-4995electronic), 2002; 41(49): 14482-14488.
Impact Factor: 4.064
View Export to BibTeX Export to EndNote
Vitagliano L, Masullo M, Sica F, Zagari A, Bocchini V
* The crystal structure of Sulfolobus solfataricus elongation factor 1α in complex with GDP reveals novel features in nucleotide binding and exchange (452 views)
Embo J (ISSN: 0261-4189, 0261-4189linking), 2001 Oct 1; 20(19): 5305-5311.
Impact Factor: 12.459
View Export to BibTeX Export to EndNote
 Esposito L, Sica F, Sorrentino G, Berisio R, Carotenuto L, Giordano A, Raia C, Rossi M, Lamzin VS, Willson KS, Zagari A
Protein Crystal Growth In The Advance Protein Crystallisation Facility On The Lms Mission: A Comparison Of Sulfolobus Solfataricus Alchool Dehydrogenase Crystals Grown On The Ground And In Microgravity (253 views)
Acta Crystallogr D Biol Crystallogr, 1998; 54: 386-390.
Impact Factor: 0
View Export to BibTeX Export to EndNote
Esposito L, Sica F, Sorrentino G, Berisio R, Carotenuto L, Giordano A, Raia CA, Rossi M, Lamzin VS, Wilson KS, Zagari A
Protein crystal growth in the advanced protein crystallization facility on the LMS mission: A comparison of Sulfolobus solfataricus alcohol dehydrogenase crystals grown on the ground and in microgravity (433 views)
Acta Crystallogr D Biol Crystallogr (ISSN: 0907-4449, 0907-4449linking), 1998; 54(3): 386-390.
Impact Factor: 2.244
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PEARL L, DEMASI D, HEMMINGS A, SICA F, MAZZARELLA L, RAIA C, DAURIA S, ROSSI M
CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF AN NAD+-DEPENDENT ALCOHOL-DEHYDROGENASE FROM THE EXTREME THERMOPHILIC ARCHAEBACTERIUM SULFOLOBUS-SOLFATARICUS (444 views)
J Mol Biol (ISSN: 0022-2836, 1089-8638, 1089-8638electronic), 1993 Feb 5; 229(3): 782-784.
Impact Factor: 5.673
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36 Records (36 excluding Abstracts).
Total impact factor: 120.396 (120.396 excluding Abstracts).
Total 5 year impact factor: 117.197 (117.197 excluding Abstracts).
Total impact factor: 120.396 (120.396 excluding Abstracts).
Total 5 year impact factor: 117.197 (117.197 excluding Abstracts).
349 views. Last view on Saturday 14 January 2023, 17:13:24
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