Characterization of carbonic anhydrase ix interactome reveals proteins assisting its nuclear localization in hypoxic cells (792 views)
J Proteome Res (ISSN: 1535-3893), 2013 Jan; 12(1): 282-292.
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Paper type: Journal Article,
Impact factor: 5.001, 5-year impact factor: 4.894
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84874104439&partnerID=40&md5=90ec0f8baeb71a319ded2d781dfef702
Keywords: Arm Repeat, Cancer, Heat Repeat, Hypoxia, Nuclear Export Signal, Nuclear Localization Signal, Carbonate Dehydratase Ix, Exportin 1, Karyopherin, Ubiquitin Protein Ligase, Article, Carboxy Terminal Sequence, Cell Nucleus, Cell Strain Hek293, Cell Surface, Cellular Distribution, Complex Formation, Cytoplasm, Down Regulation, Enzyme Analysis, Enzyme Binding, Enzyme Stability, Gene Overexpression, Genetic Transcription, Human, Hypoxic Cell, Kidney Carcinoma, Kidney Cell, Nucleotide Sequence, Priority Journal, Protein Domain, Protein Function, Protein Localization, Protein Phosphorylation, Protein Protein Interaction, Protein Stability, Protein Targeting, Antigens, Neoplasm, Carbonic Anhydrases, Renal Cell, Cell Hypoxia, Hek293 Cells, Protein Interaction Maps, Transcription Factors,
Affiliations:
*** IBB - CNR ***
CEINGE Biotecnologie Avanzate SCaRL, Naples, Italy
Proteomics and Mass Spectrometry Laboratory, ISPAAM, CNR, Naples, Italy
Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Universitä di Napoli Federico II, Italy
Istituto di Biostrutture e Bioimmagini, CNR, Naples, Italy
Dipartimento di Biologia e Patologia Cellulare e Molecolare, Universitä di Napoli Federico II, Italy
Department of Public Health, Section of Pathology, Seconda Universitä di Napoli, Italy
Dipartimento di Scienze Biomorfologiche e Funzionali, Universitä di Napoli Federico II, Italy
Department of Molecular Medicine, Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovakia
Laboratorio di Chimica Bioinorganica, Universitä di Firenze, Italy
References:
Not available.
J Proteome Res (ISSN: 1535-3893), 2013 Jan; 12(1): 282-292.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 5.001, 5-year impact factor: 4.894
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84874104439&partnerID=40&md5=90ec0f8baeb71a319ded2d781dfef702
Keywords: Arm Repeat, Cancer, Heat Repeat, Hypoxia, Nuclear Export Signal, Nuclear Localization Signal, Carbonate Dehydratase Ix, Exportin 1, Karyopherin, Ubiquitin Protein Ligase, Article, Carboxy Terminal Sequence, Cell Nucleus, Cell Strain Hek293, Cell Surface, Cellular Distribution, Complex Formation, Cytoplasm, Down Regulation, Enzyme Analysis, Enzyme Binding, Enzyme Stability, Gene Overexpression, Genetic Transcription, Human, Hypoxic Cell, Kidney Carcinoma, Kidney Cell, Nucleotide Sequence, Priority Journal, Protein Domain, Protein Function, Protein Localization, Protein Phosphorylation, Protein Protein Interaction, Protein Stability, Protein Targeting, Antigens, Neoplasm, Carbonic Anhydrases, Renal Cell, Cell Hypoxia, Hek293 Cells, Protein Interaction Maps, Transcription Factors,
Affiliations:
*** IBB - CNR ***
CEINGE Biotecnologie Avanzate SCaRL, Naples, Italy
Proteomics and Mass Spectrometry Laboratory, ISPAAM, CNR, Naples, Italy
Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Universitä di Napoli Federico II, Italy
Istituto di Biostrutture e Bioimmagini, CNR, Naples, Italy
Dipartimento di Biologia e Patologia Cellulare e Molecolare, Universitä di Napoli Federico II, Italy
Department of Public Health, Section of Pathology, Seconda Universitä di Napoli, Italy
Dipartimento di Scienze Biomorfologiche e Funzionali, Universitä di Napoli Federico II, Italy
Department of Molecular Medicine, Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovakia
Laboratorio di Chimica Bioinorganica, Universitä di Firenze, Italy
References:
Not available.
Characterization of carbonic anhydrase ix interactome reveals proteins assisting its nuclear localization in hypoxic cells
Carbonic anhydrase IX (CA IX) is a transmembrane protein affecting pH regulation, cell migration/invasion, and survival in hypoxic tumors. Although the pathways related to CA IX have begun to emerge, molecular partners mediating its functions remain largely unknown. Here we characterize the CA IX interactome in hypoxic HEK-293 cells. Most of the identified CA IX-binding partners contain the HEAT/ARM repeat domain and belong to the nuclear transport machinery. We show that the interaction with two of these proteins, namely XPO1 exportin and TNPO1 importin, occurs via the C-terminal region of CA IX and increases with protein phosphorylation. We also demonstrate that nuclear CA IX is enriched in hypoxic cells and is present in renal cell carcinoma tissues. These data place CA IX among the cell-surface signal transducers undergoing nuclear translocation. Accordingly, CA IX interactome involves also CAND1, which participates in both gene transcription and assembly of SCF ubiquitin ligase complexes. It is noteworthy that down-regulation of CAND1 leads to decreased CA IX protein levels apparently via affecting its stability. Our findings provide the first evidence that CA IX interacts with proteins involved in nuclear/cytoplasmic transport, gene transcription, and protein stability, and suggest the existence of nuclear CA IX protein subpopulations with a potential intracellular function, distinct from the crucial CA IX role at the cell surface. © 2012 American Chemical Society.
Carbonic anhydrase IX (CA IX) is a transmembrane protein affecting pH regulation, cell migration/invasion, and survival in hypoxic tumors. Although the pathways related to CA IX have begun to emerge, molecular partners mediating its functions remain largely unknown. Here we characterize the CA IX interactome in hypoxic HEK-293 cells. Most of the identified CA IX-binding partners contain the HEAT/ARM repeat domain and belong to the nuclear transport machinery. We show that the interaction with two of these proteins, namely XPO1 exportin and TNPO1 importin, occurs via the C-terminal region of CA IX and increases with protein phosphorylation. We also demonstrate that nuclear CA IX is enriched in hypoxic cells and is present in renal cell carcinoma tissues. These data place CA IX among the cell-surface signal transducers undergoing nuclear translocation. Accordingly, CA IX interactome involves also CAND1, which participates in both gene transcription and assembly of SCF ubiquitin ligase complexes. It is noteworthy that down-regulation of CAND1 leads to decreased CA IX protein levels apparently via affecting its stability. Our findings provide the first evidence that CA IX interacts with proteins involved in nuclear/cytoplasmic transport, gene transcription, and protein stability, and suggest the existence of nuclear CA IX protein subpopulations with a potential intracellular function, distinct from the crucial CA IX role at the cell surface. © 2012 American Chemical Society.
Characterization of carbonic anhydrase ix interactome reveals proteins assisting its nuclear localization in hypoxic cells
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Characterization of carbonic anhydrase ix interactome reveals proteins assisting its nuclear localization in hypoxic cells
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Supuran CT, Alterio V, Di Fiore A, D'Ambrosio K, Carta F, Monti SM, De Simone G
* Inhibition of carbonic anhydrase IX targets primary tumors, metastases, and cancer stem cells: Three for the price of one (1010 views)
Med Res Rev (ISSN: 1098-1128electronic, 0198-6325linking), 2018 Sep; 38(6): 1799-1836.
Impact Factor: 8.29
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Monti SM, Supuran CT, De Simone G
* DPP-4 inhibitors: a patent review (2012 - 2014) (916 views)
Expert Opin Ther Pat (ISSN: 1354-3776, 1744-7674electronic), 2015 Feb; 25(2): 209-236.
Impact Factor: 4.626
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Monti SM, Supuran CT, De Simone G
* Anticancer carbonic anhydrase inhibitors: A patent review (2008-2013) (808 views)
Expert Opin Ther Pat (ISSN: 1354-3776, 1744-7674electronic), 2013 Jun; 23(6): 737-749.
Impact Factor: 3.441
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Costantini S, Sharma A, Raucci R, Costantini M, Autiero I, Colonna G
Genealogy of an ancient protein family: the Sirtuins, a family of disordered members (442 views)
Bmc Evol Biol (ISSN: 1471-2148linking), 2013 Mar 5; 13: 60-60.
Impact Factor:
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Monti SM, Supuran CT, De Simone G
* Carbonic anhydrase IX as a target for designing novel anticancer drugs (1084 views)
Curr Med Chem (ISSN: 0929-8673, 1875-533x, 1875-533xelectronic), 2012 Feb; 19(6): 821-830.
Impact Factor: 4.07
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De Simone G, Supuran CT
* Carbonic anhydrase IX: Biochemical and crystallographic characterization of a novel antitumor target (837 views)
Bba-Gen Subjects (ISSN: 1570-9639, 0006-3002, 0925-4439), 2010 Feb; 1804(2): 404-409.
Impact Factor: 2.773
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Alterio V, Hilvo M, Di Fiore A, Supuran CT, Pan P, Parkkila S, Scaloni A, Pastorek J, Pastorekova S, Pedone C, Scozzafava A, Monti SM, De Simone G
* Crystal structure of the catalytic domain of the tumor-associated human carbonic anhydrase IX (1006 views)
P Natl Acad Sci Usa (ISSN: 0027-8424, 1091-6490, 0027-8424print), 2009 Sep 22; 106(38): 16233-16238.
Impact Factor: 9.432
View Export to BibTeX Export to EndNote
* Inhibition of carbonic anhydrase IX targets primary tumors, metastases, and cancer stem cells: Three for the price of one (1010 views)
Med Res Rev (ISSN: 1098-1128electronic, 0198-6325linking), 2018 Sep; 38(6): 1799-1836.
Impact Factor: 8.29
View Export to BibTeX Export to EndNote
Monti SM, Supuran CT, De Simone G
* DPP-4 inhibitors: a patent review (2012 - 2014) (916 views)
Expert Opin Ther Pat (ISSN: 1354-3776, 1744-7674electronic), 2015 Feb; 25(2): 209-236.
Impact Factor: 4.626
View Export to BibTeX Export to EndNote
Monti SM, Supuran CT, De Simone G
* Anticancer carbonic anhydrase inhibitors: A patent review (2008-2013) (808 views)
Expert Opin Ther Pat (ISSN: 1354-3776, 1744-7674electronic), 2013 Jun; 23(6): 737-749.
Impact Factor: 3.441
View Export to BibTeX Export to EndNote
Costantini S, Sharma A, Raucci R, Costantini M, Autiero I, Colonna G
Genealogy of an ancient protein family: the Sirtuins, a family of disordered members (442 views)
Bmc Evol Biol (ISSN: 1471-2148linking), 2013 Mar 5; 13: 60-60.
Impact Factor:
View Export to BibTeX Export to EndNote
Monti SM, Supuran CT, De Simone G
* Carbonic anhydrase IX as a target for designing novel anticancer drugs (1084 views)
Curr Med Chem (ISSN: 0929-8673, 1875-533x, 1875-533xelectronic), 2012 Feb; 19(6): 821-830.
Impact Factor: 4.07
View Export to BibTeX Export to EndNote
De Simone G, Supuran CT
* Carbonic anhydrase IX: Biochemical and crystallographic characterization of a novel antitumor target (837 views)
Bba-Gen Subjects (ISSN: 1570-9639, 0006-3002, 0925-4439), 2010 Feb; 1804(2): 404-409.
Impact Factor: 2.773
View Export to BibTeX Export to EndNote
Alterio V, Hilvo M, Di Fiore A, Supuran CT, Pan P, Parkkila S, Scaloni A, Pastorek J, Pastorekova S, Pedone C, Scozzafava A, Monti SM, De Simone G
* Crystal structure of the catalytic domain of the tumor-associated human carbonic anhydrase IX (1006 views)
P Natl Acad Sci Usa (ISSN: 0027-8424, 1091-6490, 0027-8424print), 2009 Sep 22; 106(38): 16233-16238.
Impact Factor: 9.432
View Export to BibTeX Export to EndNote
7 Records (7 excluding Abstracts).
Total impact factor: 32.632 (32.632 excluding Abstracts).
Total 5 year impact factor: 24.047 (24.047 excluding Abstracts).
Total impact factor: 32.632 (32.632 excluding Abstracts).
Total 5 year impact factor: 24.047 (24.047 excluding Abstracts).
792 views. Last view on Tuesday 13 May 2025, 18:59:02
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