Affiliations: Dipto. di Scienza degli Alimenti, Universita di Napoli Federico II, Via Università 100, 80055, Portici (NA), Italy
Ist. Tossine e M., CNR, Viale Einaudi 51, 70125 Bari, Italy
References: Azcona-Olivera, J.I., Abouzied, M.M., Plattner, R.D., Pestka, J.J., Production of monoclonal antibodies to the mycotoxins fumonisin-B1, fumonisins-B2 and fumonisins-B3 (1992) J. Agric. Food Chem., 40, pp. 531-53
Barna Vetro, I., Gyongyosi, A., Solti, L., Monoclonal antibody-based enzyme-linked immunosorbent assay of Fusarium T-2 and zearalenone toxins in cereals (1994) Appl. Environ. Microbiol., 60, pp. 729-731
Chanh, T.C., Huot, R.I., Schick, M.R., Hewetson, J.F., Anti-idiotypic antibodies against a monoclonal antibody specific for the trichothecene mycotoxin T-2 (1989) Toxicol. Appl. Pharmacol., 100, pp. 201-207
Chanh, T.C., Rappocciolo, G., Hewetson, J.F., Monoclonal anti-idiotype induces protection against the cytotoxicity of the trichothecene mycotoxin T-2 (1990) J. Immunol., 144, pp. 4721-4728
Chanh, T.C., Kennedy, R.C., Hewetson, J.F., Anti-idiotype vaccines in toxicology (1992) Int. J. Clin. Lab. Res., 22, pp. 28-35
Chu, F.S., Ueno, I., Production of antibodies against afla-toxin B1 (1977) Appl. Environ. Microbiol., 33, pp. 1125-1128
Chu, F.S., Chang, F.C., Hinsdill, R.D., Production of antibody against Ochratoxin A (1976) Appl. Environ. Microbiol., 31, pp. 831-835
Chu, F.S., Lau, H.P., Fan, T.S., Zhang, G.S., Ethylenediamine modified bovine serum albumin as protein carrier in the production of antiboby against mycotoxins (1982) J. Immunol. Methods, 55, pp. 73-78
Del Sorbo, G., Evidente, A., Scala, F., Production of polyclonal antibodies for cyclopaldic acid, a major phytotoxic metabolite produced by the plant pathogen Seiridium cupressi (1994) Nat. Toxins, 2, pp. 136-140
Dixon, D.E., Warner, R.L., Ram, B.P., Hart, L.P., Pestka, J.J., Hybridoma cell line production of a specific monoclonal antibody to the mycotoxins zearalenone and α-zearalenol (1987) J. Agric. Food Chem., 35, pp. 122-126
Fasciglione, G.F., Marini, S.J., Bannister, V., Giardina, B., Hapten-carrier interactions and their role in the production of monoclonal antibodies against hydrophobic haptens (1996) Hybridoma, 15, pp. 1-9
Harlow, E., Lane, D., (1988) Antibodies: A Laboratory Manual, , Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Hsu, K.H., Chu, F.S., Production and characterization of anti-idiotype and anti-idiotype antibodies from a monoclonal antibody against aflatoxin (1994) J. Agric. Food Chem., 42, pp. 2353-2359
Laëmmli, U.K., Cleavage of structural protein during the assembly of the head of bacteriofage T4 (1970) Nature (London), 227, pp. 680-685
Lau, H.P., Gaur, P.K., Chu, F.S., Preparation and characterization of aflatoxin B2a-hemiglutarate and its use for the production of antibody aganst aflatoxin B1 (1981) J. Food Saf., 3, pp. 1-13
Leslie, J.F., Pearson, C.A.S., Nelson, P.E., Toussoun, T.A., Fusarium spp. from corn, sorghum and soybean fields in the central and eastern United States (1990) Phytopathology, 80, pp. 343-350
Logrieco, A., Moretti, A., Ritieni, A., Chelkowski, J., Altomare, C., Bottalico, A., Randazzo, G., Natural occurrence of beauvericin in preharvest Fusarium subglutinans infected corn ears in Poland (1993) J. Agric. Food Chem., 41, pp. 2149-2152
McElroy, L.J., Weiss, C.M., The production of polyclonal antibodies against the mycotoxin derivative patulin hemiglutarate (1993) Can. J. Microbiol., 39, pp. 861-863
Moretti, A., Logrieco, A., Bottalico, A., Ritieni, A., Fogliano, V., Randazzo, G., Diversity in beauvericin and fusaproliferin production by different populations of Gibberella fujikuroi (Fusarium section Liseola) (1996) Sydowia, 48, pp. 44-56
Munkvold, G., Stahr, M., Logrieco, A., Moretti, A., Ritieni, A., Occurrence of fusaproliferin and beauvericin in Fusarium-contaminated livestock feed in Iowa (1998) Appl. Environ. Microbiol., 64, pp. 3923-3926
Pestka, J.J., Enhanced surveillance of foodborne mycotoxins by immunochemical assay (1988) J. Assoc. Off. Anal. Chem., 71, pp. 1075-1081
Ram, B.P., Hart, P., Shotwell, O.S., Pestka, J.J., Enzyme linked immunosorbent assay of aflatoxin B1 in naturally contamined corn and cottonseed (1986) J. Assoc. Off. Anal. Chem., 69, pp. 904-907
Randazzo, G., Fogliano, V., Ritieni, A., Mannina, L., Rossi, E., Scarallo, A., Segre, A.L., Proliferin, a new sester-terpene from Fusarium proliferatum (1993) Tetrahedron, 49, pp. 10883-10896
Ritieni, A., Monti, S.M., Randazzo, G., Logrieco, A., Bottalico, A., Peluso, G., Ferracane, R., Fogliano, V., Teratogenic effects of Fusaproliferin on chicken embryos (1997) J. Agric. Food Chem., 45, pp. 3039-3043
Ritieni, A., Moretti, A., Logrieco, A., Bottalico, A., Randazzo, G., Monti, S.M., Ferracane, R., Fogliano, V., Natural occurrence of Fusaproliferin, Fumonisin B1 and Beauvericin in preharvest Fusarium maize ear rot in Italy (1997) J. Agric. Food Chem., 45, pp. 4011-4016
Srobarova, A., Occurrence of Fusarium spp. from Slovakia maize kernels (1997) Cereal Research Communications: Proceedings of the Fifth European Fusarium Seminar, pp. 617-618. , Szeged, Hungary, August 29 - September 5. Cereal Research Institute, Szeged, Hungary. Edited by A. Mesterhazy
Towbin, H., Stahelin, T., Gordon, J., Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications (1979) Proc. Natl. Acad. Sci. U.S.A., 76, pp. 4340-4354
Azcona-Olivera, J. I., Abouzied, M. M., Plattner, R. D., Pestka, J. J., Production of monoclonal antibodies to the mycotoxins fumonisin-B1, fumonisins-B2 and fumonisins-B3 (1992) J. Agric. Food Chem., 40, pp. 531-53
Chanh, T. C., Huot, R. I., Schick, M. R., Hewetson, J. F., Anti-idiotypic antibodies against a monoclonal antibody specific for the trichothecene mycotoxin T-2 (1989) Toxicol. Appl. Pharmacol., 100, pp. 201-207
Chanh, T. C., Rappocciolo, G., Hewetson, J. F., Monoclonal anti-idiotype induces protection against the cytotoxicity of the trichothecene mycotoxin T-2 (1990) J. Immunol., 144, pp. 4721-4728
Chanh, T. C., Kennedy, R. C., Hewetson, J. F., Anti-idiotype vaccines in toxicology (1992) Int. J. Clin. Lab. Res., 22, pp. 28-35
Chu, F. S., Ueno, I., Production of antibodies against afla-toxin B1 (1977) Appl. Environ. Microbiol., 33, pp. 1125-1128
Chu, F. S., Chang, F. C., Hinsdill, R. D., Production of antibody against Ochratoxin A (1976) Appl. Environ. Microbiol., 31, pp. 831-835
Chu, F. S., Lau, H. P., Fan, T. S., Zhang, G. S., Ethylenediamine modified bovine serum albumin as protein carrier in the production of antiboby against mycotoxins (1982) J. Immunol. Methods, 55, pp. 73-78
Dixon, D. E., Warner, R. L., Ram, B. P., Hart, L. P., Pestka, J. J., Hybridoma cell line production of a specific monoclonal antibody to the mycotoxins zearalenone and -zearalenol (1987) J. Agric. Food Chem., 35, pp. 122-126
Fasciglione, G. F., Marini, S. J., Bannister, V., Giardina, B., Hapten-carrier interactions and their role in the production of monoclonal antibodies against hydrophobic haptens (1996) Hybridoma, 15, pp. 1-9
Hsu, K. H., Chu, F. S., Production and characterization of anti-idiotype and anti-idiotype antibodies from a monoclonal antibody against aflatoxin (1994) J. Agric. Food Chem., 42, pp. 2353-2359
La mmli, U. K., Cleavage of structural protein during the assembly of the head of bacteriofage T4 (1970) Nature (London), 227, pp. 680-685
Lau, H. P., Gaur, P. K., Chu, F. S., Preparation and characterization of aflatoxin B2a-hemiglutarate and its use for the production of antibody aganst aflatoxin B1 (1981) J. Food Saf., 3, pp. 1-13
Leslie, J. F., Pearson, C. A. S., Nelson, P. E., Toussoun, T. A., Fusarium spp. from corn, sorghum and soybean fields in the central and eastern United States (1990) Phytopathology, 80, pp. 343-350
McElroy, L. J., Weiss, C. M., The production of polyclonal antibodies against the mycotoxin derivative patulin hemiglutarate (1993) Can. J. Microbiol., 39, pp. 861-863
Pestka, J. J., Enhanced surveillance of foodborne mycotoxins by immunochemical assay (1988) J. Assoc. Off. Anal. Chem., 71, pp. 1075-1081
Ram, B. P., Hart, P., Shotwell, O. S., Pestka, J. J., Enzyme linked immunosorbent assay of aflatoxin B1 in naturally contamined corn and cottonseed (1986) J. Assoc. Off. Anal. Chem., 69, pp. 904-907
Polyclonal antibodies against fusaproliferin
Fusaproliferin (FP), a toxic metabolite of the world-wide maize pathogens Fusarium proliferatum and Fusarium subglutinans, was recently found to be a natural contaminant of maize. Its toxic activity on haematopoietic human cell lines and its teratogenic effects on chicken embryos has been recently proved. Therefore a sensitive, rapid, and inexpensive screening test to detect FP in agricultural commodities is necessary to protect human health. FP-hemiglutarate conjugated to modified bovine serum albumin was synthesized, characterized, and used as an antigen for raising polyclonal antibodies by immunizing rabbits. Indirect and competitive ELISA and immunoblotting analyses were performed to determine antibody specificity towards the mycotoxin. The determination of 10 μg of free FP/mL was achieved using antibodies purified by means of affinity chromatography on a FP- lysine-Sepharose column. This unsatisfactory detection limit is due to high background values; thus, this method is not competitive with traditional UV- HPLC methods.