Identification of a β-lactoglobulin lactosylation site (448 views)
Biochim Biophys Acta Protein Struct Mol Enzymol (ISSN: 0167-4838), 1998 Nov 10; 1388(2): 295-304.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 1.687, 5-year impact factor: 0
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-0032506267&partnerID=40&md5=239f7289fb31abd3dd1cdaaafb62f264
Keywords: β-Lactoglobulin, Amadori Compound, Glycosylation, Lactosylation, Liquid Chromatography-Mass Spectrometry, Maillard Reaction, Milk, Thermal Treatment, Beta Lactoglobulin, Lysine, Organic Solvent, Trypsin, Amino Acid Sequence, Article, Glycation, Heating, Ligand Binding, Molecular Model, Priority Journal, Protein Structure, Binding Sites, High Pressure Liquid, Milk Proteins, Molecular Sequence Data, Peptide Fragments,
Affiliations:
Dipto. di Scienza degli Alimenti, Univ. Napoli 'Federico II', Parco G., 80055 Portici, Naples, Italy
CRISCEB-Ctro. Ric. I., Seconda Univ. Napoli, Via C., 80138 Naples, Italy
References:
Not available.
Biochim Biophys Acta Protein Struct Mol Enzymol (ISSN: 0167-4838), 1998 Nov 10; 1388(2): 295-304.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 1.687, 5-year impact factor: 0
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-0032506267&partnerID=40&md5=239f7289fb31abd3dd1cdaaafb62f264
Keywords: β-Lactoglobulin, Amadori Compound, Glycosylation, Lactosylation, Liquid Chromatography-Mass Spectrometry, Maillard Reaction, Milk, Thermal Treatment, Beta Lactoglobulin, Lysine, Organic Solvent, Trypsin, Amino Acid Sequence, Article, Glycation, Heating, Ligand Binding, Molecular Model, Priority Journal, Protein Structure, Binding Sites, High Pressure Liquid, Milk Proteins, Molecular Sequence Data, Peptide Fragments,
Affiliations:
Dipto. di Scienza degli Alimenti, Univ. Napoli 'Federico II', Parco G., 80055 Portici, Naples, Italy
CRISCEB-Ctro. Ric. I., Seconda Univ. Napoli, Via C., 80138 Naples, Italy
References:
Not available.
Identification of a β-lactoglobulin lactosylation site
Thermal treatment of milk leads to non-enzymatic glycosylation of proteins through Maillard reaction. Free NH2 groups of basic amino acids react with the reducing carbonyl group of lactose forming the so-called Amadori products. Electrospray mass spectrometry analysis shows that β-lactoglobulin (β-LG), the major whey protein, undergoes lactosylation under industrial thermal treatment. In order to investigate the specificity of reactive sites for lactose binding the analysis of trypsin hydrolysates of β-LG isolated from different industrial milks was performed. Results demonstrate that Lys-100 is a preferential lactosylation site of β-LG during industrial milk treatment. These results were confirmed by an analysis of the three-dimensional model of the protein which showed that Lys-100 had the highest solvent accessibility and proximity to another amino group making Lys-100 the best candidate to lactosylation. Lys-47, previously identified by other authors, showed a good proximity to another Lys residue, but an intermediate level of exposition to solvent. Copyright (C) 1998 Elsevier Science B.V.
Thermal treatment of milk leads to non-enzymatic glycosylation of proteins through Maillard reaction. Free NH2 groups of basic amino acids react with the reducing carbonyl group of lactose forming the so-called Amadori products. Electrospray mass spectrometry analysis shows that β-lactoglobulin (β-LG), the major whey protein, undergoes lactosylation under industrial thermal treatment. In order to investigate the specificity of reactive sites for lactose binding the analysis of trypsin hydrolysates of β-LG isolated from different industrial milks was performed. Results demonstrate that Lys-100 is a preferential lactosylation site of β-LG during industrial milk treatment. These results were confirmed by an analysis of the three-dimensional model of the protein which showed that Lys-100 had the highest solvent accessibility and proximity to another amino group making Lys-100 the best candidate to lactosylation. Lys-47, previously identified by other authors, showed a good proximity to another Lys residue, but an intermediate level of exposition to solvent. Copyright (C) 1998 Elsevier Science B.V.
Identification of a β-lactoglobulin lactosylation site
No results. |
Identification of a β-lactoglobulin lactosylation site
Other filter: ([btitle,keywords] "β-Lactoglobulin" OR ...
Balasco N, Ferraro G, Loreto D, Iacobucci I, Monti M, Merlino A
* Cisplatin binding to β-lactoglobulin: a structural study (40 views)
Dalton T (ISSN: 1477-9226linking, 1477-9234), 2020 Sep 15; 49(35): 12450-12457.
Impact Factor: 4.174
View Export to BibTeX Export to EndNote
Di Gaetano S, Bedini E, Landolfi A, Pedone E, Pirone L, Saviano M, Traboni S, Capasso D, Iadonisi A
* Synthesis of diglycosylated (di)sulfides and comparative evaluation of their antiproliferative effect against tumor cell lines: A focus on the nature of sugar-recognizing mediators involved (139 views)
Carbohyd Res (ISSN: 0008-6215linking), 2019 Aug 1; 482: 107740-107740.
Impact Factor: 1.91
View Export to BibTeX Export to EndNote
Calce E, Digilio G, Menchise V, Saviano M, De Luca S
* Chemoselective Glycosylation of Peptides through S-Alkylation Reaction (714 views) (PDF 166 views)
Chemistry (ISSN: 1521-3765electronic, 0947-6539linking), 2018 Apr 20; 24(23): 6231-6238.
Impact Factor: 5.476
View Export to BibTeX Export to EndNote
Leiria Campo V, Riul TB, Oliveira Bortot L, Martins-teixeira MB, Fiori Marchiori M, Iaccarino E, Ruvo M, Dias-baruffi M, Carvalho I
* A Synthetic MUC1 Glycopeptide Bearing betaGalNAc-Thr as a Tn Antigen Isomer Induces the Production of Antibodies against Tumor Cells (1344 views)
Chembiochem (ISSN: 1439-4227, 1439-7633, 1439-4227linking), 2017 Mar 16; 18(6): 527-538.
Impact Factor: 2.774
View Export to BibTeX Export to EndNote
Roviello GN, Iannitti R, Roviello V, Palumbo R, Simonyan H, Vicidomini C
* Synthesis and biological evaluation of a novel Amadori compound (444 views)
Amino Acids (ISSN: 0939-4451, 1438-2199, 1438-2199electronic), 2017 Feb; 49(2): 327-335.
Impact Factor: 2.906
View Export to BibTeX Export to EndNote
Troise AD, Buonanno M, Fiore A, Monti SM, Fogliano V
* Evolution of protein bound Maillard reaction end-products and free Amadori compounds in low lactose milk in presence of fructosamine oxidase (339 views)
Food Chem (ISSN: 0308-8146, 0308-8146print), 2016 Dec 1; 212: 722-729.
Impact Factor: 4.529
View Export to BibTeX Export to EndNote
Foca A, Sanguigno L, Foca G, Strizzi L, Iannitti R, Palumbo R, Hendrix MJ, Leonardi A, Ruvo M, Sandomenico A
* New Anti-Nodal Monoclonal Antibodies Targeting the Nodal Pre-Helix Loop Involved in Cripto-1 Binding (732 views) (PDF 267 views)
Int J Mol Sc (ISSN: 1422-0067, 1661-6596, 1422-0067linking), 2015 Sep 7; 16(9): 21342-21362.
Impact Factor: 3.257
View Export to BibTeX Export to EndNote
Comegna D, de Paola I, Saviano M, Del Gatto A, Zaccaro L
* Straightforward entry to s-glycosylated fmoc-amino acids and their application to solid phase synthesis of glycopeptides and glycopeptidomimetics (519 views)
Org Lett (ISSN: 1523-7060, 1523-7052, 1523-7052electronic), 2015; 17(3): 640-643.
Impact Factor: 6.732
View Export to BibTeX Export to EndNote
Andreotti G, Pedone E, Giordano A, Cubellis MV
* Biochemical phenotype of a common disease-causing mutation and a possible therapeutic approach for the phosphomannomutase 2-associated disorder of glycosylation (271 views)
Molecular Genetics & Genomic Medicine (ISSN: 2324-9269), 2013 May; 1(1): 32-44.
Impact Factor: 2.082
View Export to BibTeX Export to EndNote
Bellia F, Pietropaolo A, Grasso G
* Formation of insulin fragments by insulin-degrading enzyme: The role of zinc(II) and cystine bridges (484 views)
J Mass Spectrom (ISSN: 1076-5174, 1096-9888), 2013; 48(2): 135-140.
Impact Factor: 2.709
View Export to BibTeX Export to EndNote
Castellano I, Merlino A
* γ-glutamyltranspeptidases: Sequence, structure, biochemical properties, and biotechnological applications (431 views)
Cell Mol Life Sci (ISSN: 1420-682x), 2012; 69(20): 3381-3394.
Impact Factor: 5.615
View Export to BibTeX Export to EndNote
Severino V, Chambery A, Di Maro A, Marasco D, Ruggiero A, Berisio R, Giansanti F, Ippoliti R, Parente A
* The role of the glycan moiety on the structure-function relationships of PD-L1, type 1 ribosome-inactivating protein from P. dioica leaves (407 views)
Mol Biosyst (ISSN: 1742-206x), 2010; 6(3): 570-579.
Impact Factor: 3.825
View Export to BibTeX Export to EndNote
Ruggiero A, Di Maro A, Severino V, Chambery A, Berisio R
* Crystal structure of PD-L1, a ribosome inactivating protein from Phytolacca dioica L. leaves with the property to induce DNA cleavage (417 views)
Biopolymers (ISSN: 0006-3525, 0006-6352, 0006-3525print), 2009 Dec; 91(12): 1135-1142.
Impact Factor: 2.605
View Export to BibTeX Export to EndNote
Hilvo M, Baranauskiene L, Salzano AM, Scaloni A, Matulis D, Innocenti A, Scozzafava A, Monti SM, Di Fiore A, De Simone G, Lindfors M, Janis J, Valjakka J, Pastorekova S, Pastorek J, Kulomaa MS, Nordlund HR, Supuran CT, Parkkila S
* Biochemical characterization of CA IX, one of the most active carbonic anhydrase isozymes (544 views)
Jbc Papers (ISSN: 0021-9258, 1083-351x, 0021-9258linking), 2008 Oct 10; 283(41): 27799-27809.
Impact Factor: 5.52
View Export to BibTeX Export to EndNote
Ruggiero A, Chambery A, Di Maro A, Mastroianni A, Parente A, Berisio R
* Crystallization and preliminary X-ray diffraction analysis of PD-L1, a highly glycosylated ribosome inactivating protein with DNase activity (618 views)
Protein Pept Lett (ISSN: 0929-8665, 1875-5305), 2007; 14(4): 407-409.
Impact Factor: 1.097
View Export to BibTeX Export to EndNote
Fogliano V, Monti SM, Ritieni A, Marchisano C, Peluso G, Randazzo G
An immunological approach to monitor protein lactosylation of heated food model systems (352 views)
Food Chem (ISSN: 0308-8146, 0308-8146print), 1997; 58(1-2): 53-58.
Impact Factor: 0.812
View Export to BibTeX Export to EndNote
Latella G, Fonti R, Caprilli R, Marcheggiano A, Magliocca F, Das KM, Gambús G, Sambuy Y
Characterization of the mucins produced by normal human colonocytes in primary culture (282 views)
International Journal Of Colorectal Disease (ISSN: 0179-1958, 0179-1958linking), 1996; 11(2): 76-83.
Impact Factor: 0.732
View Export to BibTeX Export to EndNote
* Cisplatin binding to β-lactoglobulin: a structural study (40 views)
Dalton T (ISSN: 1477-9226linking, 1477-9234), 2020 Sep 15; 49(35): 12450-12457.
Impact Factor: 4.174
View Export to BibTeX Export to EndNote
Di Gaetano S, Bedini E, Landolfi A, Pedone E, Pirone L, Saviano M, Traboni S, Capasso D, Iadonisi A
* Synthesis of diglycosylated (di)sulfides and comparative evaluation of their antiproliferative effect against tumor cell lines: A focus on the nature of sugar-recognizing mediators involved (139 views)
Carbohyd Res (ISSN: 0008-6215linking), 2019 Aug 1; 482: 107740-107740.
Impact Factor: 1.91
View Export to BibTeX Export to EndNote
Calce E, Digilio G, Menchise V, Saviano M, De Luca S
* Chemoselective Glycosylation of Peptides through S-Alkylation Reaction (714 views) (PDF 166 views)
Chemistry (ISSN: 1521-3765electronic, 0947-6539linking), 2018 Apr 20; 24(23): 6231-6238.
Impact Factor: 5.476
View Export to BibTeX Export to EndNote
Leiria Campo V, Riul TB, Oliveira Bortot L, Martins-teixeira MB, Fiori Marchiori M, Iaccarino E, Ruvo M, Dias-baruffi M, Carvalho I
* A Synthetic MUC1 Glycopeptide Bearing betaGalNAc-Thr as a Tn Antigen Isomer Induces the Production of Antibodies against Tumor Cells (1344 views)
Chembiochem (ISSN: 1439-4227, 1439-7633, 1439-4227linking), 2017 Mar 16; 18(6): 527-538.
Impact Factor: 2.774
View Export to BibTeX Export to EndNote
Roviello GN, Iannitti R, Roviello V, Palumbo R, Simonyan H, Vicidomini C
* Synthesis and biological evaluation of a novel Amadori compound (444 views)
Amino Acids (ISSN: 0939-4451, 1438-2199, 1438-2199electronic), 2017 Feb; 49(2): 327-335.
Impact Factor: 2.906
View Export to BibTeX Export to EndNote
Troise AD, Buonanno M, Fiore A, Monti SM, Fogliano V
* Evolution of protein bound Maillard reaction end-products and free Amadori compounds in low lactose milk in presence of fructosamine oxidase (339 views)
Food Chem (ISSN: 0308-8146, 0308-8146print), 2016 Dec 1; 212: 722-729.
Impact Factor: 4.529
View Export to BibTeX Export to EndNote
Foca A, Sanguigno L, Foca G, Strizzi L, Iannitti R, Palumbo R, Hendrix MJ, Leonardi A, Ruvo M, Sandomenico A
* New Anti-Nodal Monoclonal Antibodies Targeting the Nodal Pre-Helix Loop Involved in Cripto-1 Binding (732 views) (PDF 267 views)
Int J Mol Sc (ISSN: 1422-0067, 1661-6596, 1422-0067linking), 2015 Sep 7; 16(9): 21342-21362.
Impact Factor: 3.257
View Export to BibTeX Export to EndNote
Comegna D, de Paola I, Saviano M, Del Gatto A, Zaccaro L
* Straightforward entry to s-glycosylated fmoc-amino acids and their application to solid phase synthesis of glycopeptides and glycopeptidomimetics (519 views)
Org Lett (ISSN: 1523-7060, 1523-7052, 1523-7052electronic), 2015; 17(3): 640-643.
Impact Factor: 6.732
View Export to BibTeX Export to EndNote
Andreotti G, Pedone E, Giordano A, Cubellis MV
* Biochemical phenotype of a common disease-causing mutation and a possible therapeutic approach for the phosphomannomutase 2-associated disorder of glycosylation (271 views)
Molecular Genetics & Genomic Medicine (ISSN: 2324-9269), 2013 May; 1(1): 32-44.
Impact Factor: 2.082
View Export to BibTeX Export to EndNote
Bellia F, Pietropaolo A, Grasso G
* Formation of insulin fragments by insulin-degrading enzyme: The role of zinc(II) and cystine bridges (484 views)
J Mass Spectrom (ISSN: 1076-5174, 1096-9888), 2013; 48(2): 135-140.
Impact Factor: 2.709
View Export to BibTeX Export to EndNote
Castellano I, Merlino A
* γ-glutamyltranspeptidases: Sequence, structure, biochemical properties, and biotechnological applications (431 views)
Cell Mol Life Sci (ISSN: 1420-682x), 2012; 69(20): 3381-3394.
Impact Factor: 5.615
View Export to BibTeX Export to EndNote
Severino V, Chambery A, Di Maro A, Marasco D, Ruggiero A, Berisio R, Giansanti F, Ippoliti R, Parente A
* The role of the glycan moiety on the structure-function relationships of PD-L1, type 1 ribosome-inactivating protein from P. dioica leaves (407 views)
Mol Biosyst (ISSN: 1742-206x), 2010; 6(3): 570-579.
Impact Factor: 3.825
View Export to BibTeX Export to EndNote
Ruggiero A, Di Maro A, Severino V, Chambery A, Berisio R
* Crystal structure of PD-L1, a ribosome inactivating protein from Phytolacca dioica L. leaves with the property to induce DNA cleavage (417 views)
Biopolymers (ISSN: 0006-3525, 0006-6352, 0006-3525print), 2009 Dec; 91(12): 1135-1142.
Impact Factor: 2.605
View Export to BibTeX Export to EndNote
Hilvo M, Baranauskiene L, Salzano AM, Scaloni A, Matulis D, Innocenti A, Scozzafava A, Monti SM, Di Fiore A, De Simone G, Lindfors M, Janis J, Valjakka J, Pastorekova S, Pastorek J, Kulomaa MS, Nordlund HR, Supuran CT, Parkkila S
* Biochemical characterization of CA IX, one of the most active carbonic anhydrase isozymes (544 views)
Jbc Papers (ISSN: 0021-9258, 1083-351x, 0021-9258linking), 2008 Oct 10; 283(41): 27799-27809.
Impact Factor: 5.52
View Export to BibTeX Export to EndNote
Ruggiero A, Chambery A, Di Maro A, Mastroianni A, Parente A, Berisio R
* Crystallization and preliminary X-ray diffraction analysis of PD-L1, a highly glycosylated ribosome inactivating protein with DNase activity (618 views)
Protein Pept Lett (ISSN: 0929-8665, 1875-5305), 2007; 14(4): 407-409.
Impact Factor: 1.097
View Export to BibTeX Export to EndNote
Fogliano V, Monti SM, Ritieni A, Marchisano C, Peluso G, Randazzo G
An immunological approach to monitor protein lactosylation of heated food model systems (352 views)
Food Chem (ISSN: 0308-8146, 0308-8146print), 1997; 58(1-2): 53-58.
Impact Factor: 0.812
View Export to BibTeX Export to EndNote
Latella G, Fonti R, Caprilli R, Marcheggiano A, Magliocca F, Das KM, Gambús G, Sambuy Y
Characterization of the mucins produced by normal human colonocytes in primary culture (282 views)
International Journal Of Colorectal Disease (ISSN: 0179-1958, 0179-1958linking), 1996; 11(2): 76-83.
Impact Factor: 0.732
View Export to BibTeX Export to EndNote
17 Records (17 excluding Abstracts).
Total impact factor: 56.755 (56.755 excluding Abstracts).
Total 5 year impact factor: 60.612 (60.612 excluding Abstracts).
Total impact factor: 56.755 (56.755 excluding Abstracts).
Total 5 year impact factor: 60.612 (60.612 excluding Abstracts).
448 views. Last view on Tuesday 23 May 2023, 2:48:04
ibb.cnr.it
