Acetylation on critical lysine residues of Apurinic/apyrimidinic endonuclease 1 (APE1) in triple negative breast cancers (919 views)
Biochemical And Biophysical Research Communications (ISSN: 0006-291x), 2012 Jul 20; 424(1): 34-39.
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Paper type: Journal Article,
Impact factor: 2.406, 5-year impact factor: 2.5
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84864083812&partnerID=40&md5=1133b5d5cf41896e4c4d8429ce8533f2
Keywords: Acetylation, Apurinic Apyrimidinic Endonuclease Redox Effector Factor 1 (ape1 Ref-1), Base Excision Repair (ber), Triple Negative Breast Cancer (tnbc), Lysine Derivative, Polyclonal Antibody, Article, Cancer Prognosis, Cohort Analysis, Controlled Study, Female, Human, Human Cell, Immunofluorescence, Immunohistochemistry, Priority Journal, Protein Synthesis, Amino Acid Sequence, Breast Neoplasms, Dna-(apurinic Or Apyrimidinic Site) Lyase, Molecular Sequence Data, Protein Processing, Post-Translational, Tumor Cells, Cultured, Mammalia,
Affiliations:
*** IBB - CNR ***
Department of Medical and Biological Sciences, University of Udine, 33100 Udine, Italy
Department of Biological Sciences, University of Naples Federico II, 80134 Naples, Italy
Institute of Biostructures and Bioimaging, National Research Council, 80134 Naples, Italy
References:
Not available.
Biochemical And Biophysical Research Communications (ISSN: 0006-291x), 2012 Jul 20; 424(1): 34-39.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 2.406, 5-year impact factor: 2.5
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84864083812&partnerID=40&md5=1133b5d5cf41896e4c4d8429ce8533f2
Keywords: Acetylation, Apurinic Apyrimidinic Endonuclease Redox Effector Factor 1 (ape1 Ref-1), Base Excision Repair (ber), Triple Negative Breast Cancer (tnbc), Lysine Derivative, Polyclonal Antibody, Article, Cancer Prognosis, Cohort Analysis, Controlled Study, Female, Human, Human Cell, Immunofluorescence, Immunohistochemistry, Priority Journal, Protein Synthesis, Amino Acid Sequence, Breast Neoplasms, Dna-(apurinic Or Apyrimidinic Site) Lyase, Molecular Sequence Data, Protein Processing, Post-Translational, Tumor Cells, Cultured, Mammalia,
Affiliations:
*** IBB - CNR ***
Department of Medical and Biological Sciences, University of Udine, 33100 Udine, Italy
Department of Biological Sciences, University of Naples Federico II, 80134 Naples, Italy
Institute of Biostructures and Bioimaging, National Research Council, 80134 Naples, Italy
References:
Not available.
Acetylation on critical lysine residues of Apurinic/apyrimidinic endonuclease 1 (APE1) in triple negative breast cancers
Protein acetylation plays many roles within living cells, modulating metabolism, signaling and cell response to environmental stimuli, as well as having an impact on pathological conditions, such as cancer pathogenesis and progression. The Apurinic/apyrimidinic endonuclease APE1 is a vital protein that exerts many functions in mammalian cells, acting as a pivotal enzyme in the base excision repair (BER) pathway of DNA lesions, as transcriptional modulator and being also involved in RNA metabolism. As an eclectic and abundant protein, APE1 is extensively modulated through post-translational modifications, including acetylation. Many findings have linked APE1 to cancer development and onset of chemo- and radio-resistance. Here, we focus on APE1 acetylation pattern in triple negative breast cancer (TNBC). We describe the validation and characterization of a polyclonal antibody that is specific for the acetylation on lysine 35 of the protein. Finally, we use the new antibody to analyze the APE1 acetylation pattern on a cohort of TNBC specimens, exploiting immunohistochemistry. Our findings reveal a profound deregulation of APE1 acetylation status in TNBC, opening new perspectives for future improvements on treatment and prognosis of this molecular subtype of breast carcinomas. (C) 2012 Elsevier Inc. All rights reserved.
Protein acetylation plays many roles within living cells, modulating metabolism, signaling and cell response to environmental stimuli, as well as having an impact on pathological conditions, such as cancer pathogenesis and progression. The Apurinic/apyrimidinic endonuclease APE1 is a vital protein that exerts many functions in mammalian cells, acting as a pivotal enzyme in the base excision repair (BER) pathway of DNA lesions, as transcriptional modulator and being also involved in RNA metabolism. As an eclectic and abundant protein, APE1 is extensively modulated through post-translational modifications, including acetylation. Many findings have linked APE1 to cancer development and onset of chemo- and radio-resistance. Here, we focus on APE1 acetylation pattern in triple negative breast cancer (TNBC). We describe the validation and characterization of a polyclonal antibody that is specific for the acetylation on lysine 35 of the protein. Finally, we use the new antibody to analyze the APE1 acetylation pattern on a cohort of TNBC specimens, exploiting immunohistochemistry. Our findings reveal a profound deregulation of APE1 acetylation status in TNBC, opening new perspectives for future improvements on treatment and prognosis of this molecular subtype of breast carcinomas. (C) 2012 Elsevier Inc. All rights reserved.
Acetylation on critical lysine residues of Apurinic/apyrimidinic endonuclease 1 (APE1) in triple negative breast cancers
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Acetylation on critical lysine residues of Apurinic/apyrimidinic endonuclease 1 (APE1) in triple negative breast cancers
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View Export to BibTeX Export to EndNote
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Impact Factor: 3.348
View Export to BibTeX Export to EndNote
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Impact Factor: 4.881
View Export to BibTeX Export to EndNote
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Impact Factor: 3.274
View Export to BibTeX Export to EndNote
Poletto M, Vascotto C, Scognamiglio PL, Lirussi L, Marasco D, Tell G
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Impact Factor: 4.779
View Export to BibTeX Export to EndNote
Lirussi L, Antoniali G, Vascotto C, D'Ambrosio C, Poletto M, Romanello M, Marasco D, Leone M, Quadrifoglio F, Bhakat KK, Scaloni A, Tell G
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Impact Factor: 4.803
View Export to BibTeX Export to EndNote
Calabrese V, Cornelius C, Cuzzocrea S, Iavicoli I, Rizzarelli E, Calabrese EJ
* Hormesis, cellular stress response and vitagenes as critical determinants in aging and longevity (1292 views)
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Impact Factor: 9.97
View Export to BibTeX Export to EndNote
Milite C, Castellano S, Benedetti R, Tosco A, Ciliberti C, Vicidomini C, Boully L, Franci G, Altucci L, Mai A, Sbardella G
* Modulation of the activity of histone acetyltransferases by long chain alkylidenemalonates (LoCAMs) (607 views)
Bioorg Med Chem (ISSN: 0968-0896, 1464-3391, 1464-3391electronic), 2011 Jun 15; 19(12): 3690-3701.
Impact Factor: 2.921
View Export to BibTeX Export to EndNote
Fantini D, Vascotto C, Marasco D, D'Ambrosio C, Romanello M, Vitagliano L, Pedone C, Poletto M, Cesaratto L, Quadrifoglio F, Scaloni A, Radicella JP, Tell G
* Critical lysine residues within the overlooked N-terminal domain of human APE1 regulate its biological functions (814 views)
Nucleic Acids Res (ISSN: 1362-4962, 0305-1048, 1362-4962electronic), 2010 Dec; 38(22): 8239-8256.
Impact Factor: 7.836
View Export to BibTeX Export to EndNote
Canettieri G, Di Marcotullio L, Greco A, Coni S, Antonucci L, Infante P, Pietrosanti L, De Smaele E, Ferretti E, Miele E, Pelloni M, De Simone G, Pedone E, Gallinari P, Giorgi A, Steinkuhler C, Vitagliano L, Pedone C, Schinin ME, Screpanti I, Gulino A
* Histone deacetylase and Cullin3-REN(KCTD11) ubiquitin ligase interplay regulates Hedgehog signalling through Gli acetylation (1252 views)
Nature Cell Biol Nature Cell Biology (ISSN: 1476-4679, 1465-7392), 2010 Feb; 12(2): 132-142.
Impact Factor: 19.407
View Export to BibTeX Export to EndNote
Chiechio S, Zammataro M, Morales ME, Busceti CL, Drago F, Th Gereau RW, Copani A, Nicoletti F
* Epigenetic modulation of mGlu2 receptors by histone deacetylase inhibitors in the treatment of inflammatory pain (1108 views)
Mol Pharmacol (ISSN: 0026-895x), 2009; 75(5): 1014-1020.
Impact Factor: 4.531
View Export to BibTeX Export to EndNote
Sbardella G, Castellano S, Vicidomini C, Rotili D, Nebbioso A, Miceli M, Altucci L, Mai A
* Identification of long chain alkylidenemalonates as novel small molecule modulators of histone acetyltransferases (838 views)
Bioorganic & Medicinal Chemistry Letters (ISSN: 0960-894x), 2008 May 1; 18(9): 2788-2792.
Impact Factor: 2.531
View Export to BibTeX Export to EndNote
Mai A, Rotili D, Tarantino D, Ornaghi P, Tosi F, Vicidomini C, Sbardella G, Nebbioso A, Miceli M, Altucci L, Filetici P
* Small-molecule inhibitors of histone acetyltransferase activity: Identification and biological properties (1003 views)
J Med Chem (ISSN: 0022-2623, 1520-4804, 0022-2623print), 2006 Nov 16; 49(23): 6897-6907.
Impact Factor: 5.115
View Export to BibTeX Export to EndNote
Mai A, Rotili D, Ornaghi P, Tosi F, Vicidomini C, Sardella G, Nebbioso A, Altucci L, Filatici P
* Identification of small molecules inhibitors of GCN5 histone acetyltransferase activity (604 views)
Arkivoc (ISSN: 1424-6376), 2006; 6(VIII): 24-37.
Impact Factor: 0.8
View Export to BibTeX Export to EndNote
Brown DR, Guantieri V, Grasso G, Impellizzeri G, Pappalardo G, Rizzarelli E
* Copper(II) complexes of peptide fragments of the prion protein. Conformation changes induced by copper(II) and the binding motif in C-terminal protein region (1079 views)
J Chem Res (ISSN: 0162-0134, 1873-3344, 0162-0134print), 2004 Jan; 98(1): 133-143.
Impact Factor: 2.225
View Export to BibTeX Export to EndNote
De Napoli L, De Luca S, Di Fabio G, Messere A, Montesarchio D, Morelli G, Piccialli G, Tesauro D
A facile solid-phase strategy for the synthesis of oligonucleotide- tetraphenylporphyrin conjugates (782 views)
European Journal Of Organic Chemistry (ISSN: 1434-193x), 2000; (6): 1013-1018.
Impact Factor: 2.15
View Export to BibTeX Export to EndNote
18 Records (18 excluding Abstracts).
Total impact factor: 95.145 (95.145 excluding Abstracts).
Total 5 year impact factor: 99.849 (99.849 excluding Abstracts).
Total impact factor: 95.145 (95.145 excluding Abstracts).
Total 5 year impact factor: 99.849 (99.849 excluding Abstracts).
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