DETERMINATION OF ANTIGEN CONCENTRATION AND DISTRIBUTION IN HUMAN TUMORS THE QUANTITATIVE AUTORADIOGRAPHIC APPROACH(602 views) Del Vecchio S, Reynolds JC
Nucl Med Biol (ISSN: 0969-8051, 0883-2897), 1989; 16(2): 143-145.
Keywords: Monoclonal Antibody I 125, Radioisotope, Tumor Antigen, Clinical Article, Histochemistry, Human, Human Cell, Melanoma, Methodology, Priority Journal, Neoplasm, Autoradiography,
Affiliations: Nuclear Medicine Department, Clinical Center, National Institutes of Health, Bethesda, MD 20892, United States
References: Not available.
DETERMINATION OF ANTIGEN CONCENTRATION AND DISTRIBUTION IN HUMAN TUMORS THE QUANTITATIVE AUTORADIOGRAPHIC APPROACH
We developed a technique that uses 125I labeled monoclonal antibody and in vitro quantitative autoradiography to measure antigen concentration in histologic sections of human tumors. Unlike techniques that disrupt tumors such as quantitative analysis of homogenates of fluorescence activated cell sorter analysis, in vitro quantitative autoradiography preserves tissue anatomy. In this way it allows us to study tumors in the same conditions used for immunoperoxidase staining providing additional numerical data on antigen concentration.
DETERMINATION OF ANTIGEN CONCENTRATION AND DISTRIBUTION IN HUMAN TUMORS THE QUANTITATIVE AUTORADIOGRAPHIC APPROACH
No results.
DETERMINATION OF ANTIGEN CONCENTRATION AND DISTRIBUTION IN HUMAN TUMORS THE QUANTITATIVE AUTORADIOGRAPHIC APPROACH
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