A facile solid-phase strategy for the synthesis of oligonucleotide- tetraphenylporphyrin conjugates(417 views) De Napoli L, De Luca S, Di Fabio G, Messere A, Montesarchio D, Morelli G, Piccialli G, Tesauro D
European Journal Of Organic Chemistry (ISSN: 1434-193x), 2000; (6): 1013-1018.
Affiliations: Dipto. di Chim. Organica e Biologica, Univ. Studi di Napoli Federico II, Via Mezzocannone 16, 80134 Napoli, Italy
Ctro. Interuniversitario Ric. P., Ctro. di Studi di Biocristallografia, Via Mezzocannone 4, 80134 Napoli, Italy
Università del Molise, Facoltà di Scienze, Via Mazzini 8, 86170 Isernia, Italy
Universit del Molise, Facolt di Scienze, Via Mazzini 8, 86170 Isernia, Italy
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A facile solid-phase strategy for the synthesis of oligonucleotide- tetraphenylporphyrin conjugates
A convenient solid-phase synthesis of oligonucleotides conjugated at the 3'-end with a tetraphenylporphyrin residue, by means of a new polymeric support bearing as a linker a lysine derivative, has been developed. A porphyrin linked 17-mer, designed for antisense experiments, has been prepared in good yields, and its hybridization properties with a complementary DNA fragment evaluated by UV thermal analysis.
A facile solid-phase strategy for the synthesis of oligonucleotide- tetraphenylporphyrin conjugates