Measurement of P-glycoprotein expression in human neuroblastoma xenografts using in vitro quantitative autoradiography (327 views)
Nucl Med Biol (ISSN: 0969-8051, 0883-2897), 1999 Jan; 26(1): 35-41.
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Paper type: Journal Article,
Impact factor: 1.623, 5-year impact factor: 2.442
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-0032891335&partnerID=40&md5=4f03b29e8e055cd92d87b0175450d18b
Keywords: Mrk16, Multidrug Resistance, P-Glycoprotein, Quantitative Autoradiography, Self-Competitive Binding Assay, Iodine 125, Monoclonal Antibody Mrk 16, Animal Experiment, Article, Cancer Cell Culture, Cancer Transplantation, Human, Human Cell, Isotope Labeling, Mouse, Neuroblastoma, Nonhuman, Nude Mouse, Protein Determination, Protein Expression, Quantitative Assay, Radioassay, Tissue Distribution, Antibodies, Multiple, Iodine Radioisotopes, Inbred Balb C, Neoplasm Transplantation, Heterologous, Tumor Cells,
Affiliations:
Department of Nuclear Medicine, Mem. Sloan-Kettering Cancer C., New York, NY, United States
Inst. of Molec. and Cell. Biosci., University of Tokyo, Tokyo, Japan
References:
Not available.
Nucl Med Biol (ISSN: 0969-8051, 0883-2897), 1999 Jan; 26(1): 35-41.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 1.623, 5-year impact factor: 2.442
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-0032891335&partnerID=40&md5=4f03b29e8e055cd92d87b0175450d18b
Keywords: Mrk16, Multidrug Resistance, P-Glycoprotein, Quantitative Autoradiography, Self-Competitive Binding Assay, Iodine 125, Monoclonal Antibody Mrk 16, Animal Experiment, Article, Cancer Cell Culture, Cancer Transplantation, Human, Human Cell, Isotope Labeling, Mouse, Neuroblastoma, Nonhuman, Nude Mouse, Protein Determination, Protein Expression, Quantitative Assay, Radioassay, Tissue Distribution, Antibodies, Multiple, Iodine Radioisotopes, Inbred Balb C, Neoplasm Transplantation, Heterologous, Tumor Cells,
Affiliations:
Department of Nuclear Medicine, Mem. Sloan-Kettering Cancer C., New York, NY, United States
Inst. of Molec. and Cell. Biosci., University of Tokyo, Tokyo, Japan
References:
Not available.
Measurement of P-glycoprotein expression in human neuroblastoma xenografts using in vitro quantitative autoradiography
P-glycoprotein (P-gp) has a role in multidrug resistance (MDR) encountered in human cancers. In this study, we used the colchicine-resistant cell line BE(2)-C/CHCb(0.2), a strain of neuroblastoma cell line BE(2)-C, as a model to measure variations of P-gp expression in cells grown in vitro and in vivo. Cells were cultured in the medium supplemented with colchicine. At the beginning of the study the drug was withdrawn and, after 22 days, added back to the culture medium. Cells were harvested at various time points and xenografted in nude mice. P-gp COntent in cells was measured by self- competitive binding assay and in tumors, by quantitative autoradiography (QAR). Both assays were carried out using 125I-labeled monoclonal antibody MRK16, reactive with P-gp. Concentration of P-gp in cells varied from a maximum of 1,361 pmol/g in the presence of colchicine to a minimum of 374 pmol/g in the absence of colchicine in the culture medium. P-gp concentration in the tumors ranged from 929 to 188 pmol/g, which correlated with P-gp content in the cells at the time of their injection in the mice. QAR is an accurate and reliable method to quantify P-gp expression in tumors. Changes in colchicine concentration in the ambient medium of BE(2)-C/CHCb(0.2) cells growing in vitro resulted in a change in phenotype of P-gp expression, which was stable under conditions of in vivo growth over approximately 9 cell divisions in nude mice xenografts. Therefore, P-gp content in xenografts depends only on the level of resistance of the cells at the time of their injection in the mice.
P-glycoprotein (P-gp) has a role in multidrug resistance (MDR) encountered in human cancers. In this study, we used the colchicine-resistant cell line BE(2)-C/CHCb(0.2), a strain of neuroblastoma cell line BE(2)-C, as a model to measure variations of P-gp expression in cells grown in vitro and in vivo. Cells were cultured in the medium supplemented with colchicine. At the beginning of the study the drug was withdrawn and, after 22 days, added back to the culture medium. Cells were harvested at various time points and xenografted in nude mice. P-gp COntent in cells was measured by self- competitive binding assay and in tumors, by quantitative autoradiography (QAR). Both assays were carried out using 125I-labeled monoclonal antibody MRK16, reactive with P-gp. Concentration of P-gp in cells varied from a maximum of 1,361 pmol/g in the presence of colchicine to a minimum of 374 pmol/g in the absence of colchicine in the culture medium. P-gp concentration in the tumors ranged from 929 to 188 pmol/g, which correlated with P-gp content in the cells at the time of their injection in the mice. QAR is an accurate and reliable method to quantify P-gp expression in tumors. Changes in colchicine concentration in the ambient medium of BE(2)-C/CHCb(0.2) cells growing in vitro resulted in a change in phenotype of P-gp expression, which was stable under conditions of in vivo growth over approximately 9 cell divisions in nude mice xenografts. Therefore, P-gp content in xenografts depends only on the level of resistance of the cells at the time of their injection in the mice.
Measurement of P-glycoprotein expression in human neuroblastoma xenografts using in vitro quantitative autoradiography
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Measurement of P-glycoprotein expression in human neuroblastoma xenografts using in vitro quantitative autoradiography
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View Export to BibTeX Export to EndNote
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View Export to BibTeX Export to EndNote
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View Export to BibTeX Export to EndNote
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J Nucl Med (ISSN: 1535-5667, 0161-5505, 1535-5667electronic), 2006 Mar; 47(3): 555-555.
Impact Factor: 4.986
View Export to BibTeX Export to EndNote
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Pace L, Del Vecchio S, Salvatore M
* Technetium 99m sestamibi in multiple myeloma (277 views)
Radiology (ISSN: 0033-8419, 1527-1315, 1527-1315electronic), 2005 Jan; 234(1): 312-313.
Impact Factor: 5.377
View Export to BibTeX Export to EndNote
Pace L, Del Vecchio S, Salvatore M, Walker RC, Angtuaco E, Sethi R, Fassas A, Barlogie B
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Impact Factor: 5.377
View Export to BibTeX Export to EndNote
Del Vecchio S, Salvatore M
* Tc-99m-MIBI in the evaluation of breast cancer biology (632 views) (PDF 182 views)
Eur J Nucl Med (ISSN: 1619-7070, 1619-7089, 0340-6997), 2004 Jun; 31(SUPPL.1): S88-S88.
Impact Factor: 3.935
View Export to BibTeX Export to EndNote
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Impact Factor: 1.091
View Export to BibTeX Export to EndNote
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Impact Factor: 2.863
View Export to BibTeX Export to EndNote
Del Vecchio S, Zannetti A, Aloj L, Salvatore M
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Impact Factor: 2.222
View Export to BibTeX Export to EndNote
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Impact Factor: 3.568
View Export to BibTeX Export to EndNote
Del Vecchio S, Ciarmiello A, Salvatore M
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Impact Factor: 0.989
View Export to BibTeX Export to EndNote
Del Vecchio S, Ciarmiello A, Salvatore M
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Q J Nucl Med (ISSN: 1124-3937, 1125-0135), 1999 Jun; 43(2): 125-131.
Impact Factor: 2.125
View Export to BibTeX Export to EndNote
Renda A, Iovino F, Capasso L, Ricciardelli L, Tammaro V, Acampa W
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Impact Factor: 1.045
View Export to BibTeX Export to EndNote
Salvatore M, Del Vecchio S
Dynamic imaging: Scintimammography (399 views)
Eur J Radiol Elsevier Sci Ireland Ltd (ISSN: 0720-048x), 1998 May; 27(SUPPL.2): S259-S259.
Impact Factor: 0.537
View Export to BibTeX Export to EndNote
Mansi L, Rambaldi PF, Cuccurullo V, Pecori B, Quarantelli M, Fallanca F, Del Vecchio E
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Q J Nucl Med (ISSN: 1124-3937, 1125-0135), 1997 Sep; 41(3): 239-250.
Impact Factor: 2.125
View Export to BibTeX Export to EndNote
Del Vecchio S, Ciarmiello A, Pace L, Potena MI, Carriero MV, Mainolfi C, Thomas R, D'Aiuto G, Tsuruo T, Salvatore M
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J Nucl Med (ISSN: 0161-5505, 1535-5667, 1535-5667electronic), 1997 Sep; 38(9): 1348-1351.
Impact Factor: 3.491
View Export to BibTeX Export to EndNote
Carriero MV, Del Vecchio S, Franco P, Potena MI, Chiaradonna F, Botti G, Stoppelli MP, Salvatore M
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Impact Factor: 3.419
View Export to BibTeX Export to EndNote
Levchenko A, Mehta B, Spengler BA, Narkar A, Fonti R, Biedler JL, Tsuruo T, Larson SM
Measurement of P-glycoprotein expression in multidrug-resistant human neuroblastoma cell lines using self-competitive binding assay (284 views)
Anal Biochem (ISSN: 0003-2697, 1096-0309electronic, 0003-2697linking), 1996 May 1; 236(2): 338-343.
Impact Factor: 2.017
View Export to BibTeX Export to EndNote
Li PY, Del Vecchio S, Fonti R, Carriero MV, Potena MI, Botti G, Miotti S, Lastoria S, Menard S, Colnaghi MI, Salvatore M
Local concentration of folate binding protein GP38 in sections of human ovarian carcinoma by in vitro quantitative autoradiography (468 views)
J Nucl Med (ISSN: 0161-5505, 1535-5667, 1535-5667electronic), 1996 Apr; 37(4-6): 665-672.
Impact Factor: 3.491
View Export to BibTeX Export to EndNote
Del Vecchio S, Ciarmiello A, Potena MI, Carriero MV, Mainolfi C, Botti G, Tsuruo T, Salvatore M
Quantitation of P-glycoprotein in sections of human breast carcinoma (299 views)
J Nucl Med (ISSN: 0161-5505, 1535-5667, 1535-5667electronic), 1995; 36(5SUPPL.): N/D-N/D.
Impact Factor: 3.491
View Export to BibTeX Export to EndNote
Ciarmiello A, Del Vecchio S, Potena MI, Mainolfi C, Carriero MV, Tsuruo T, Marone A, Salvatore M
Tc-99m-SESTAMIBI efflux and P-glycoprotein expression in human breast carcinoma (261 views)
J Nucl Med (ISSN: 0161-5505, 1535-5667, 1535-5667electronic), 1995; 36(5SUPPL.): N/D-N/D.
Impact Factor: 3.491
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Del Vecchio S, Stoppelli MP, Carriero MV, Fonti R, Massa O, Li PY, Botti G, Cerra M, D'Aiuto G, Esposito G, Salvatore M
IN-VITRO RECEPTOR IMAGING FOR CHARACTERIZATION OF HUMAN SOLID TUMORS (271 views)
Nucl Med Biol (ISSN: 0883-2897, 0969-8051), 1994 Jul; 21(5): 771-774.
Impact Factor: 1.042
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Correale P, Caraglia M, Pepe S, Ricciardi B, Barile C, Montagnani S, Pacelli R, Bianco A, Tagliaferri P
PHORBOL 12-MYRISTATE 13-ACETATE (PMA) INDUCES NEUROENDOCRINE-LIKE DIFFERENTIATION AND REVERSES DOXORUBICIN-RESISTANCE OF HUMAN COLON-CARCINOMA CELLS IN-VITRO (303 views)
Int J Oncol (ISSN: 1019-6439, 1791-2423electronic, 1019-6439linking), 1994 Feb; 4(2): 423-427.
Impact Factor: 1.181
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Del Vecchio S, Stoppelli MP, Carriere MV, Fonti R, Massa O, Li PY, Botti G, Cerra M, D'Aiuto G, Esposito G, Salvatore M
Human urokinase receptor concentration in malignant and benign breast tumors by in vitro quantitative autoradiography: Comparison with urokinase levels (293 views)
Cancer Res (ISSN: 0008-5472, 1538-7445, 1538-7445electronic), 1993 Jul 1; 53(13): 3198-3206.
Impact Factor: 8.426
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Del Vecchio S, Reynolds JC, Blasberg RG, Neumann RD, Carrasquillo JA, Hellström I, Larson SM
Measurement of local Mr 97, 000 and 250, 000 protein antigen concentration in sections of human melanoma tumor using in vitro quantitative autoradiography (528 views)
Cancer Res (ISSN: 0008-5472, 1538-7445, 1538-7445electronic), 1988; 48(19): 5475-5481.
Impact Factor: 8.426
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Trischitta V, Benzi L, Brunetti A, Cecchetti P, Marchetti P, Vigneri R, Navalesi R
Intracellular insulin processing is altered in monocytes from patients with type II diabetes mellitus (971 views)
J Clin Endocrinol Metab (ISSN: 0021-972x), 1987 May; 64(5): 914-920.
Impact Factor: 4.575
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31 Records (26 excluding Abstracts).
Total impact factor: 99.917 (83.477 excluding Abstracts).
Total 5 year impact factor: 122.281 (98.653 excluding Abstracts).
Total impact factor: 99.917 (83.477 excluding Abstracts).
Total 5 year impact factor: 122.281 (98.653 excluding Abstracts).
327 views. Last view on Sunday 18 June 2023, 7:23:40
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