Anti-amyloidogenic property of human gastrokine (843 views)
Biochimie (ISSN: 0300-9084, 1638-6183, 0300-9084linking), 2014 Nov; 106C: 91-100.
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Paper type: Journal Article, Research Support, Non-U. S. Gov'T,
Impact factor: 2.963, 5-year impact factor: 3.124
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84908005465&partnerID=40&md5=8f0ecc092b7da77c3db3ba71919b5454
Keywords: Amyloidogenesis, Aβ Peptide, Brichos Domain, Gastric Cancer, Gastrokine 1, Amyloid Beta Protein[1-40], Amyloid Precursor Protein, Chaperone, Cystatin, Nitrilotriacetate Nickel, Polypeptide, Recombinant Gastrokine 1, Recombinant Protein, Surfactant Protein C, Thioflavine, Unclassified Drug, Article, Binding Assay, Confocal Microscopy, Controlled Study, Drug Activity, Fiber, Human, Human Cell, Hydrolysis, Mass Spectrometry, Neuroblastoma Cell Line, Polyacrylamide Gel Electrophoresis, Polymerization, Protein Aggregation, Protein Binding, Protein Expression, Protein Protein Interaction, Protein Secondary Structure, Stomach Adenocarcinoma, Surface Plasmon Resonance, Z Peptide, Aß Peptide, A Peptide, Amyloid Beta Protein [1-40], Amino Acid Sequence , Amyloid Chemistry Metabolism , Amyloid Beta-Peptides Chemistry Metabolism , Amyloid Beta-Protein Precursor Metabolism , Tumor , Molecular Sequence Data , Peptide Fragments Chemistry Metabolism , Peptide Hormones Genetics Metabolism Pharmacology , Protein Aggregates Drug Effects , Recombinant Proteins Metabolism Pharmacology , Sequence Homology, Matrix-Assisted Laser Desorption-Ionization,
Affiliations:
*** IBB - CNR ***
Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, Via S. Pansini 5Naples, Italy
Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of NaplesCaserta, Italy
Institute of Biostructures and Bioimaging-IBB, CNRNaples, Italy
IRCCS MultimedicaMilan, Italy
Laboratory of Biotechnology, Department of Anesthesia, Surgical and Emergency Sciences, Second University of Naples, Via Costantinopoli 16Naples, Italy
Department of Motor Sciences and Wellness, University of ParthenopeNaples, Italy
CEINGE, Advanced Biotechnology Scarl, Via Gaetano Salvatore 486Naples, Italy
IRCCS Multimedica, Milan, Italy
References:
Not available.
Biochimie (ISSN: 0300-9084, 1638-6183, 0300-9084linking), 2014 Nov; 106C: 91-100.
Export to BibTeX Export to EndNote
Paper type: Journal Article, Research Support, Non-U. S. Gov'T,
Impact factor: 2.963, 5-year impact factor: 3.124
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84908005465&partnerID=40&md5=8f0ecc092b7da77c3db3ba71919b5454
Keywords: Amyloidogenesis, Aβ Peptide, Brichos Domain, Gastric Cancer, Gastrokine 1, Amyloid Beta Protein[1-40], Amyloid Precursor Protein, Chaperone, Cystatin, Nitrilotriacetate Nickel, Polypeptide, Recombinant Gastrokine 1, Recombinant Protein, Surfactant Protein C, Thioflavine, Unclassified Drug, Article, Binding Assay, Confocal Microscopy, Controlled Study, Drug Activity, Fiber, Human, Human Cell, Hydrolysis, Mass Spectrometry, Neuroblastoma Cell Line, Polyacrylamide Gel Electrophoresis, Polymerization, Protein Aggregation, Protein Binding, Protein Expression, Protein Protein Interaction, Protein Secondary Structure, Stomach Adenocarcinoma, Surface Plasmon Resonance, Z Peptide, Aß Peptide, A Peptide, Amyloid Beta Protein [1-40], Amino Acid Sequence , Amyloid Chemistry Metabolism , Amyloid Beta-Peptides Chemistry Metabolism , Amyloid Beta-Protein Precursor Metabolism , Tumor , Molecular Sequence Data , Peptide Fragments Chemistry Metabolism , Peptide Hormones Genetics Metabolism Pharmacology , Protein Aggregates Drug Effects , Recombinant Proteins Metabolism Pharmacology , Sequence Homology, Matrix-Assisted Laser Desorption-Ionization,
Affiliations:
*** IBB - CNR ***
Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, Via S. Pansini 5Naples, Italy
Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of NaplesCaserta, Italy
Institute of Biostructures and Bioimaging-IBB, CNRNaples, Italy
IRCCS MultimedicaMilan, Italy
Laboratory of Biotechnology, Department of Anesthesia, Surgical and Emergency Sciences, Second University of Naples, Via Costantinopoli 16Naples, Italy
Department of Motor Sciences and Wellness, University of ParthenopeNaples, Italy
CEINGE, Advanced Biotechnology Scarl, Via Gaetano Salvatore 486Naples, Italy
IRCCS Multimedica, Milan, Italy
References:
Not available.
Anti-amyloidogenic property of human gastrokine
Gastrokine 1 (GKN1) is a stomach-specific protein expressed in normal gastric tissue but absent in gastric cancer. GKN1 plays a major role in maintaining gastric mucosa integrity and is characterized by the presence of a BRICHOS domain consisting of about 100 amino acids also found in several unrelated proteins associated with major human diseases like BRI2, related to familial British and Danish dementia and surfactant protein C (SP-C), associated with respiratory distress syndrome. It was reported that recombinant BRICHOS domains from BRI2 and SP-C precursor (proSP-C) prevent fibrils formation of amyloid-beta peptide (A beta), that is the major component of extracellular amyloid deposits in Alzheimer's disease. Here we investigated on the interaction between human recombinant GKN1 (rGKN1) and A beta peptide (1-40) that derives from the partial hydrolysis of the amyloid precursor protein (APP). GKN1 prevented amyloid aggregation and fibrils formation by inhibiting A beta (1-40) polymerization, as evaluated by SDS-PAGE, thioflavin-T binding assay and gel filtration experiments. Mass spectrometry showed the formation of a prevailing 1: 1 complex between GKN1 and A beta (1-40). SPR analysis of GKN1/A beta interaction led to calculate a dissociation constant (K-D) of 34 mu M. Besides its interaction with A beta (1-40), GKN1 showed also to interact with APP as evaluated by confocal microscopy and Ni-NTA pull-down. Data strongly suggest that GKN1 has anti-amyloidogenic properties thus functioning as a chaperone directed against unfolded segments and with the ability to recognize amyloidogenic polypeptides and prevent their aggregation. (C) 2014 Elsevier B. V. and Societe francaise de biochimie et biologie Moleculaire (SFBBM). All rights reserved
Gastrokine 1 (GKN1) is a stomach-specific protein expressed in normal gastric tissue but absent in gastric cancer. GKN1 plays a major role in maintaining gastric mucosa integrity and is characterized by the presence of a BRICHOS domain consisting of about 100 amino acids also found in several unrelated proteins associated with major human diseases like BRI2, related to familial British and Danish dementia and surfactant protein C (SP-C), associated with respiratory distress syndrome. It was reported that recombinant BRICHOS domains from BRI2 and SP-C precursor (proSP-C) prevent fibrils formation of amyloid-beta peptide (A beta), that is the major component of extracellular amyloid deposits in Alzheimer's disease. Here we investigated on the interaction between human recombinant GKN1 (rGKN1) and A beta peptide (1-40) that derives from the partial hydrolysis of the amyloid precursor protein (APP). GKN1 prevented amyloid aggregation and fibrils formation by inhibiting A beta (1-40) polymerization, as evaluated by SDS-PAGE, thioflavin-T binding assay and gel filtration experiments. Mass spectrometry showed the formation of a prevailing 1: 1 complex between GKN1 and A beta (1-40). SPR analysis of GKN1/A beta interaction led to calculate a dissociation constant (K-D) of 34 mu M. Besides its interaction with A beta (1-40), GKN1 showed also to interact with APP as evaluated by confocal microscopy and Ni-NTA pull-down. Data strongly suggest that GKN1 has anti-amyloidogenic properties thus functioning as a chaperone directed against unfolded segments and with the ability to recognize amyloidogenic polypeptides and prevent their aggregation. (C) 2014 Elsevier B. V. and Societe francaise de biochimie et biologie Moleculaire (SFBBM). All rights reserved
Anti-amyloidogenic property of human gastrokine
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Anti-amyloidogenic property of human gastrokine
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7 Records (7 excluding Abstracts).
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Total impact factor: 148.957 (148.957 excluding Abstracts).
Total 5 year impact factor: 122.327 (122.327 excluding Abstracts).
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