Anti-amyloidogenic property of human gastrokine (531 views)
Biochimie (ISSN: 0300-9084, 1638-6183, 0300-9084linking), 2014 Nov; 106C: 91-100.
Export to BibTeX Export to EndNote
Paper type: Journal Article, Research Support, Non-U. S. Gov'T,
Impact factor: 2.963, 5-year impact factor: 3.124
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84908005465&partnerID=40&md5=8f0ecc092b7da77c3db3ba71919b5454
Keywords: Amyloidogenesis, Aβ Peptide, Brichos Domain, Gastric Cancer, Gastrokine 1, Amyloid Beta Protein[1-40], Amyloid Precursor Protein, Chaperone, Cystatin, Nitrilotriacetate Nickel, Polypeptide, Recombinant Gastrokine 1, Recombinant Protein, Surfactant Protein C, Thioflavine, Unclassified Drug, Article, Binding Assay, Confocal Microscopy, Controlled Study, Drug Activity, Fiber, Human, Human Cell, Hydrolysis, Mass Spectrometry, Neuroblastoma Cell Line, Polyacrylamide Gel Electrophoresis, Polymerization, Protein Aggregation, Protein Binding, Protein Expression, Protein Protein Interaction, Protein Secondary Structure, Stomach Adenocarcinoma, Surface Plasmon Resonance, Z Peptide, Aß Peptide, A Peptide, Amyloid Beta Protein [1-40], Amino Acid Sequence , Amyloid Chemistry Metabolism , Amyloid Beta-Peptides Chemistry Metabolism , Amyloid Beta-Protein Precursor Metabolism , Tumor , Molecular Sequence Data , Peptide Fragments Chemistry Metabolism , Peptide Hormones Genetics Metabolism Pharmacology , Protein Aggregates Drug Effects , Recombinant Proteins Metabolism Pharmacology , Sequence Homology, Matrix-Assisted Laser Desorption-Ionization,
Affiliations:
*** IBB - CNR ***
Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, Via S. Pansini 5Naples, Italy
Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of NaplesCaserta, Italy
Institute of Biostructures and Bioimaging-IBB, CNRNaples, Italy
IRCCS MultimedicaMilan, Italy
Laboratory of Biotechnology, Department of Anesthesia, Surgical and Emergency Sciences, Second University of Naples, Via Costantinopoli 16Naples, Italy
Department of Motor Sciences and Wellness, University of ParthenopeNaples, Italy
CEINGE, Advanced Biotechnology Scarl, Via Gaetano Salvatore 486Naples, Italy
IRCCS Multimedica, Milan, Italy
References:
Not available.
Biochimie (ISSN: 0300-9084, 1638-6183, 0300-9084linking), 2014 Nov; 106C: 91-100.
Export to BibTeX Export to EndNote
Paper type: Journal Article, Research Support, Non-U. S. Gov'T,
Impact factor: 2.963, 5-year impact factor: 3.124
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84908005465&partnerID=40&md5=8f0ecc092b7da77c3db3ba71919b5454
Keywords: Amyloidogenesis, Aβ Peptide, Brichos Domain, Gastric Cancer, Gastrokine 1, Amyloid Beta Protein[1-40], Amyloid Precursor Protein, Chaperone, Cystatin, Nitrilotriacetate Nickel, Polypeptide, Recombinant Gastrokine 1, Recombinant Protein, Surfactant Protein C, Thioflavine, Unclassified Drug, Article, Binding Assay, Confocal Microscopy, Controlled Study, Drug Activity, Fiber, Human, Human Cell, Hydrolysis, Mass Spectrometry, Neuroblastoma Cell Line, Polyacrylamide Gel Electrophoresis, Polymerization, Protein Aggregation, Protein Binding, Protein Expression, Protein Protein Interaction, Protein Secondary Structure, Stomach Adenocarcinoma, Surface Plasmon Resonance, Z Peptide, Aß Peptide, A Peptide, Amyloid Beta Protein [1-40], Amino Acid Sequence , Amyloid Chemistry Metabolism , Amyloid Beta-Peptides Chemistry Metabolism , Amyloid Beta-Protein Precursor Metabolism , Tumor , Molecular Sequence Data , Peptide Fragments Chemistry Metabolism , Peptide Hormones Genetics Metabolism Pharmacology , Protein Aggregates Drug Effects , Recombinant Proteins Metabolism Pharmacology , Sequence Homology, Matrix-Assisted Laser Desorption-Ionization,
Affiliations:
*** IBB - CNR ***
Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, Via S. Pansini 5Naples, Italy
Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of NaplesCaserta, Italy
Institute of Biostructures and Bioimaging-IBB, CNRNaples, Italy
IRCCS MultimedicaMilan, Italy
Laboratory of Biotechnology, Department of Anesthesia, Surgical and Emergency Sciences, Second University of Naples, Via Costantinopoli 16Naples, Italy
Department of Motor Sciences and Wellness, University of ParthenopeNaples, Italy
CEINGE, Advanced Biotechnology Scarl, Via Gaetano Salvatore 486Naples, Italy
IRCCS Multimedica, Milan, Italy
References:
Not available.
Anti-amyloidogenic property of human gastrokine
Gastrokine 1 (GKN1) is a stomach-specific protein expressed in normal gastric tissue but absent in gastric cancer. GKN1 plays a major role in maintaining gastric mucosa integrity and is characterized by the presence of a BRICHOS domain consisting of about 100 amino acids also found in several unrelated proteins associated with major human diseases like BRI2, related to familial British and Danish dementia and surfactant protein C (SP-C), associated with respiratory distress syndrome. It was reported that recombinant BRICHOS domains from BRI2 and SP-C precursor (proSP-C) prevent fibrils formation of amyloid-beta peptide (A beta), that is the major component of extracellular amyloid deposits in Alzheimer's disease. Here we investigated on the interaction between human recombinant GKN1 (rGKN1) and A beta peptide (1-40) that derives from the partial hydrolysis of the amyloid precursor protein (APP). GKN1 prevented amyloid aggregation and fibrils formation by inhibiting A beta (1-40) polymerization, as evaluated by SDS-PAGE, thioflavin-T binding assay and gel filtration experiments. Mass spectrometry showed the formation of a prevailing 1: 1 complex between GKN1 and A beta (1-40). SPR analysis of GKN1/A beta interaction led to calculate a dissociation constant (K-D) of 34 mu M. Besides its interaction with A beta (1-40), GKN1 showed also to interact with APP as evaluated by confocal microscopy and Ni-NTA pull-down. Data strongly suggest that GKN1 has anti-amyloidogenic properties thus functioning as a chaperone directed against unfolded segments and with the ability to recognize amyloidogenic polypeptides and prevent their aggregation. (C) 2014 Elsevier B. V. and Societe francaise de biochimie et biologie Moleculaire (SFBBM). All rights reserved
Gastrokine 1 (GKN1) is a stomach-specific protein expressed in normal gastric tissue but absent in gastric cancer. GKN1 plays a major role in maintaining gastric mucosa integrity and is characterized by the presence of a BRICHOS domain consisting of about 100 amino acids also found in several unrelated proteins associated with major human diseases like BRI2, related to familial British and Danish dementia and surfactant protein C (SP-C), associated with respiratory distress syndrome. It was reported that recombinant BRICHOS domains from BRI2 and SP-C precursor (proSP-C) prevent fibrils formation of amyloid-beta peptide (A beta), that is the major component of extracellular amyloid deposits in Alzheimer's disease. Here we investigated on the interaction between human recombinant GKN1 (rGKN1) and A beta peptide (1-40) that derives from the partial hydrolysis of the amyloid precursor protein (APP). GKN1 prevented amyloid aggregation and fibrils formation by inhibiting A beta (1-40) polymerization, as evaluated by SDS-PAGE, thioflavin-T binding assay and gel filtration experiments. Mass spectrometry showed the formation of a prevailing 1: 1 complex between GKN1 and A beta (1-40). SPR analysis of GKN1/A beta interaction led to calculate a dissociation constant (K-D) of 34 mu M. Besides its interaction with A beta (1-40), GKN1 showed also to interact with APP as evaluated by confocal microscopy and Ni-NTA pull-down. Data strongly suggest that GKN1 has anti-amyloidogenic properties thus functioning as a chaperone directed against unfolded segments and with the ability to recognize amyloidogenic polypeptides and prevent their aggregation. (C) 2014 Elsevier B. V. and Societe francaise de biochimie et biologie Moleculaire (SFBBM). All rights reserved
Anti-amyloidogenic property of human gastrokine
No results. |
Anti-amyloidogenic property of human gastrokine
Other filter: ([btitle,keywords] "Amyloidogenesis" OR ...
Russi S, Verma HK, Laurino S, Mazzone P, Storto G, Nardelli A, Zoppoli P, Calice G, La Rocca F, Sgambato A, Lucci V, Falco G, Ruggieri V
* Adapting and Surviving: Intra and Extra-Cellular Remodeling in Drug-Resistant Gastric Cancer Cells (186 views)
Int J Mol Sc (ISSN: 1422-0067linking, 1661-6596, 1422-0067electronic), 2019 Jul 31; 20(15): N/D-N/D.
Impact Factor: 4.183
View Export to BibTeX Export to EndNote
Ribaldone DG, Fagoonee S, Astegiano M, Durazzo M, Morgando A, Sprujevnik T, Giordanino C, Baronio M, De Angelis C, Saracco GM, Pellicano R
* Rifabutin-Based Rescue Therapy for Helicobacter pylori Eradication: A Long-Term Prospective Study in a Large Cohort of Difficult-to-Treat Patients (293 views)
J Clin Med (ISSN: 2077-0383linking, 2077-0383electronic), 2019 Feb 6; 8(2): N/D-N/D.
Impact Factor: 5.583
View Export to BibTeX Export to EndNote
Pellicano R, Fagoonee S
* Helicobacter pylori and Prevention of Gastric Cancer (232 views)
N Engl J Med (ISSN: 0028-4793linking, 1533-4406electronic), 2018 Jun 7; 378(23): 2244-2244.
Impact Factor: 72.406
View Export to BibTeX Export to EndNote
Fagoonee S, Durazzo M
* HOTAIR and gastric cancer: a lesson from two meta-analyses (155 views)
Panminerva Med (ISSN: 0031-0808, 1827-1898electronic, 0031-0808linking), 2017 Sep; 59(3): 201-202.
Impact Factor: 2.102
View Export to BibTeX Export to EndNote
Kim YH, Shin SW, Pellicano R, Fagoonee S, Choi IJ, Kim YI, Park B, Choi JM, Kim SG, Choi J, Park JY, Oh S, Yang HJ, Lim JH, Im JP, Kim JS, Jung HC, Ponzetto A, Figura N, Malfertheiner P, Choi IJ, Kook MC, Kim YI, Cho SJ, Lee JY, Kim CG, Park B, Nam BH, Bae SE, Choi KD, Choe J, Kim SO, Na HK, Choi JY, Ahn JY, Jung KW, Lee J, Kim DH, Chang HS, Song HJ, Lee GH, Jung HY, Seta T, Takahashi Y, Noguchi Y, Shikata S, Sakai T, Sakai K, Yamashita Y, Nakayama T, Leja M, Park JY, Murillo R, Liepniece-karele I, Isajevs S, Kikuste I, Rudzite D, Krike P, Parshutin S, Polaka I, Kirsners A, Santare D, Folkmanis V, Daugule I, Plummer M, Herrero R, Tsukamoto T, Nakagawa M, Kiriyama Y, Toyoda T, Cao X, Corral JE, Mera R, Dye CW, Morgan DR, Lee YC, Lin JT, Garcia Martin R, Matia Cubillo A, Lee SH, Park JM, Han YM, Ko WJ, Hahm KB, Leontiadis GI, Ford AC, Ichinose M, Sugano K, Jeong M, Park JM, Han YM, Park KY, Lee DH, Yoo JH, Cho JY, Hahm KB, Bang CS, Baik GH, Shin IS, Kim JB, Suk KT, Yoon JH, Kim YS, Kim DJ
* Helicobacter pylori Eradication for Prevention of Metachronous Recurrence after Endoscopic Resection of Early Gastric Cancer (413 views)
N Engl J Med (ISSN: 0028-4793, 0028-4793linking, 1533-4406electronic), 2015 Jun; 30642104201566393291: 749-756.
Impact Factor: 59.558
View Export to BibTeX Export to EndNote
Pannuzzo M, Milardi D, Raudino A, Karttunen M, La Rosa C
* Analytical model and multiscale simulations of Aβ peptide aggregation in lipid membranes: Towards a unifying description of conformational transitions, oligomerization and membrane damage (780 views)
Phys Chem Chem Phys (ISSN: 1463-9076, 1463-3907, 1463-9084electronic), 2013; 15(23): 8940-8951.
Impact Factor: 4.198
View Export to BibTeX Export to EndNote
Esposito G, Tempesta A, Galati MG, De Bellis M, Rossi GB, Potena MI, Marone P, Daniele B, Del Vecchio S
Mucosal expression of carcinoembryonic antigen and carbohydrate antigen 19-9 in patients with gastritis and gastric cancer (324 views)
Cancer Detect Prev (ISSN: 0361-090x, 0361-090xlinking), 1999; 23(2): 116-122.
Impact Factor: 0.927
View Export to BibTeX Export to EndNote
* Adapting and Surviving: Intra and Extra-Cellular Remodeling in Drug-Resistant Gastric Cancer Cells (186 views)
Int J Mol Sc (ISSN: 1422-0067linking, 1661-6596, 1422-0067electronic), 2019 Jul 31; 20(15): N/D-N/D.
Impact Factor: 4.183
View Export to BibTeX Export to EndNote
Ribaldone DG, Fagoonee S, Astegiano M, Durazzo M, Morgando A, Sprujevnik T, Giordanino C, Baronio M, De Angelis C, Saracco GM, Pellicano R
* Rifabutin-Based Rescue Therapy for Helicobacter pylori Eradication: A Long-Term Prospective Study in a Large Cohort of Difficult-to-Treat Patients (293 views)
J Clin Med (ISSN: 2077-0383linking, 2077-0383electronic), 2019 Feb 6; 8(2): N/D-N/D.
Impact Factor: 5.583
View Export to BibTeX Export to EndNote
Pellicano R, Fagoonee S
* Helicobacter pylori and Prevention of Gastric Cancer (232 views)
N Engl J Med (ISSN: 0028-4793linking, 1533-4406electronic), 2018 Jun 7; 378(23): 2244-2244.
Impact Factor: 72.406
View Export to BibTeX Export to EndNote
Fagoonee S, Durazzo M
* HOTAIR and gastric cancer: a lesson from two meta-analyses (155 views)
Panminerva Med (ISSN: 0031-0808, 1827-1898electronic, 0031-0808linking), 2017 Sep; 59(3): 201-202.
Impact Factor: 2.102
View Export to BibTeX Export to EndNote
Kim YH, Shin SW, Pellicano R, Fagoonee S, Choi IJ, Kim YI, Park B, Choi JM, Kim SG, Choi J, Park JY, Oh S, Yang HJ, Lim JH, Im JP, Kim JS, Jung HC, Ponzetto A, Figura N, Malfertheiner P, Choi IJ, Kook MC, Kim YI, Cho SJ, Lee JY, Kim CG, Park B, Nam BH, Bae SE, Choi KD, Choe J, Kim SO, Na HK, Choi JY, Ahn JY, Jung KW, Lee J, Kim DH, Chang HS, Song HJ, Lee GH, Jung HY, Seta T, Takahashi Y, Noguchi Y, Shikata S, Sakai T, Sakai K, Yamashita Y, Nakayama T, Leja M, Park JY, Murillo R, Liepniece-karele I, Isajevs S, Kikuste I, Rudzite D, Krike P, Parshutin S, Polaka I, Kirsners A, Santare D, Folkmanis V, Daugule I, Plummer M, Herrero R, Tsukamoto T, Nakagawa M, Kiriyama Y, Toyoda T, Cao X, Corral JE, Mera R, Dye CW, Morgan DR, Lee YC, Lin JT, Garcia Martin R, Matia Cubillo A, Lee SH, Park JM, Han YM, Ko WJ, Hahm KB, Leontiadis GI, Ford AC, Ichinose M, Sugano K, Jeong M, Park JM, Han YM, Park KY, Lee DH, Yoo JH, Cho JY, Hahm KB, Bang CS, Baik GH, Shin IS, Kim JB, Suk KT, Yoon JH, Kim YS, Kim DJ
* Helicobacter pylori Eradication for Prevention of Metachronous Recurrence after Endoscopic Resection of Early Gastric Cancer (413 views)
N Engl J Med (ISSN: 0028-4793, 0028-4793linking, 1533-4406electronic), 2015 Jun; 30642104201566393291: 749-756.
Impact Factor: 59.558
View Export to BibTeX Export to EndNote
Pannuzzo M, Milardi D, Raudino A, Karttunen M, La Rosa C
* Analytical model and multiscale simulations of Aβ peptide aggregation in lipid membranes: Towards a unifying description of conformational transitions, oligomerization and membrane damage (780 views)
Phys Chem Chem Phys (ISSN: 1463-9076, 1463-3907, 1463-9084electronic), 2013; 15(23): 8940-8951.
Impact Factor: 4.198
View Export to BibTeX Export to EndNote
Esposito G, Tempesta A, Galati MG, De Bellis M, Rossi GB, Potena MI, Marone P, Daniele B, Del Vecchio S
Mucosal expression of carcinoembryonic antigen and carbohydrate antigen 19-9 in patients with gastritis and gastric cancer (324 views)
Cancer Detect Prev (ISSN: 0361-090x, 0361-090xlinking), 1999; 23(2): 116-122.
Impact Factor: 0.927
View Export to BibTeX Export to EndNote
7 Records (7 excluding Abstracts).
Total impact factor: 148.957 (148.957 excluding Abstracts).
Total 5 year impact factor: 122.327 (122.327 excluding Abstracts).
Total impact factor: 148.957 (148.957 excluding Abstracts).
Total 5 year impact factor: 122.327 (122.327 excluding Abstracts).
531 views. Last view on Sunday 09 July 2023, 2:15:41
ibb.cnr.it
