The Collagen Binding Domain of Gelatinase A Modulates Degradation of Collagen IV by Gelatinase (523 views)
J Mol Biol (ISSN: 0022-2836, 1089-8638, 1089-8638electronic), 2009; 386(2): 419-434.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 3.871, 5-year impact factor: 4.303
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-59149094419&partnerID=40&md5=bb0287fbd44ec55112d6b69147937514
Keywords: Collagen Binding Domain, Gelatinase B, Kinetics, Matrix Metalloproteinases, Type Iv Collagen Fragmentation, Collagen Type 4, Fibronectin, Matrix Metalloproteinase Inhibitor, Analytic Method, Article, Binding Site, Catalysis, Cell Migration, Collagen Degradation, Conformational Transition, Controlled Study, Edman Sequencing, Enzyme Activity, Human, Human Cell, Mass Spectrometry, Neutrophil, Placenta, Priority Journal, Protein Binding, Protein Degradation, Protein Domain, Surface Plasmon Resonance, Environmental Monitoring, Peptides, Protein Interaction Mapping, Sequence Analysis, Temperature,
Affiliations:
*** IBB - CNR ***
Department of Experimental Medicine and Biochemical Sciences, University of Roma Tor Vergata, Via Montpellier 1, I-00133 Rome, Italy
Department of Oral Biological and Medical Sciences, Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC V6T1Z3, Canada
Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC V6T1Z3, Canada
Interuniversity Consortium for the Research on the Chemistry of Metals in Biological Systems, P.za Umberto I 1, I-70100 Bari, Italy
Rega Institute for Medical Research, Laboratory of Immunobiology, University of Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium
Department of Chemistry, University of Catania, V.le A. Doria, Catania, Italy
Institute of Biostructure and Bioimaging, CNR, V.le A. Doria, Catania, Italy
References:
Not available.
J Mol Biol (ISSN: 0022-2836, 1089-8638, 1089-8638electronic), 2009; 386(2): 419-434.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 3.871, 5-year impact factor: 4.303
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-59149094419&partnerID=40&md5=bb0287fbd44ec55112d6b69147937514
Keywords: Collagen Binding Domain, Gelatinase B, Kinetics, Matrix Metalloproteinases, Type Iv Collagen Fragmentation, Collagen Type 4, Fibronectin, Matrix Metalloproteinase Inhibitor, Analytic Method, Article, Binding Site, Catalysis, Cell Migration, Collagen Degradation, Conformational Transition, Controlled Study, Edman Sequencing, Enzyme Activity, Human, Human Cell, Mass Spectrometry, Neutrophil, Placenta, Priority Journal, Protein Binding, Protein Degradation, Protein Domain, Surface Plasmon Resonance, Environmental Monitoring, Peptides, Protein Interaction Mapping, Sequence Analysis, Temperature,
Affiliations:
*** IBB - CNR ***
Department of Experimental Medicine and Biochemical Sciences, University of Roma Tor Vergata, Via Montpellier 1, I-00133 Rome, Italy
Department of Oral Biological and Medical Sciences, Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC V6T1Z3, Canada
Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC V6T1Z3, Canada
Interuniversity Consortium for the Research on the Chemistry of Metals in Biological Systems, P.za Umberto I 1, I-70100 Bari, Italy
Rega Institute for Medical Research, Laboratory of Immunobiology, University of Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium
Department of Chemistry, University of Catania, V.le A. Doria, Catania, Italy
Institute of Biostructure and Bioimaging, CNR, V.le A. Doria, Catania, Italy
References:
Not available.
The Collagen Binding Domain of Gelatinase A Modulates Degradation of Collagen IV by Gelatinase
Type IV collagen remodeling plays a critical role in inflammatory responses, angiogenesis and metastasis. Its remodeling is executed by a family of matrix metalloproteinases (MMPs), of which the constitutive gelatinase A (MMP2) and the inducible gelatinase B (MMP9) are key examples. Thus, in many pathological conditions, both gelatinases act together. Kinetic data are reported for the enzymatic processing at 37 °C of type IV collagen from human placenta by MMP9 and its modulation by the fibronectin-like collagen binding domain (CBD) of MMP2. The α1 and α2 chain components of type IV collagen were cleaved by gelatinases and identified by mass spectrometry as well as Edman sequencing. Surface plasmon resonance interaction assays showed that CBD bound type IV collagen at two topologically distinct sites. On the basis of linked-function analysis, we demonstrated that CBD of MMP2 tuned the cleavage of collagen IV by MMP9, presumably by inducing a ligand-linked structural change on the type IV collagen. At low concentrations, the CBD bound the first site and thereby allosterically modulated the binding of MMP9 to collagen IV, thus enhancing the collagenolytic activity of MMP9. At high concentrations, CBD binding to the second site interfered with MMP9 binding to collagen IV, acting as a competitive inhibitor. Interestingly, modulation of collagen IV degradation by inactive forms of MMP2 also occurred in a cell-based system, revealing that this interrelationship affected neutrophil migration across a collagen IV membrane. The regulation of the proteolytic processing by a catalytically inactive domain (i.e., CBD) suggests that the two gelatinases might cooperate in degrading substrates even when either one is inactive. This observation reinforces the idea of exosite targets for MMP inhibitors, which should include all macromolecular substrate recognition sites. © 2008 Elsevier Ltd. All rights reserved.
Type IV collagen remodeling plays a critical role in inflammatory responses, angiogenesis and metastasis. Its remodeling is executed by a family of matrix metalloproteinases (MMPs), of which the constitutive gelatinase A (MMP2) and the inducible gelatinase B (MMP9) are key examples. Thus, in many pathological conditions, both gelatinases act together. Kinetic data are reported for the enzymatic processing at 37 °C of type IV collagen from human placenta by MMP9 and its modulation by the fibronectin-like collagen binding domain (CBD) of MMP2. The α1 and α2 chain components of type IV collagen were cleaved by gelatinases and identified by mass spectrometry as well as Edman sequencing. Surface plasmon resonance interaction assays showed that CBD bound type IV collagen at two topologically distinct sites. On the basis of linked-function analysis, we demonstrated that CBD of MMP2 tuned the cleavage of collagen IV by MMP9, presumably by inducing a ligand-linked structural change on the type IV collagen. At low concentrations, the CBD bound the first site and thereby allosterically modulated the binding of MMP9 to collagen IV, thus enhancing the collagenolytic activity of MMP9. At high concentrations, CBD binding to the second site interfered with MMP9 binding to collagen IV, acting as a competitive inhibitor. Interestingly, modulation of collagen IV degradation by inactive forms of MMP2 also occurred in a cell-based system, revealing that this interrelationship affected neutrophil migration across a collagen IV membrane. The regulation of the proteolytic processing by a catalytically inactive domain (i.e., CBD) suggests that the two gelatinases might cooperate in degrading substrates even when either one is inactive. This observation reinforces the idea of exosite targets for MMP inhibitors, which should include all macromolecular substrate recognition sites. © 2008 Elsevier Ltd. All rights reserved.
The Collagen Binding Domain of Gelatinase A Modulates Degradation of Collagen IV by Gelatinase
No results. |
The Collagen Binding Domain of Gelatinase A Modulates Degradation of Collagen IV by Gelatinase
Other filter: ([btitle,keywords] "Collagen Binding Dom ...
Squeglia F, Ruggiero A, Berisio R
* Collagen degradation in tuberculosis pathogenesis: the biochemical consequences of hosting an undesired guest (574 views)
Biochem J (ISSN: 1470-8728electronic, 0264-6021linking, 0264-6021print), 2018 Oct 11; 475(19): 3123-3140.
Impact Factor: 4.897
View Export to BibTeX Export to EndNote
Zinnhardt B, Viel T, Wachsmuth L, Vrachimis A, Wagner S, Breyholz HJ, Faust A, Hermann S, Kopka K, Faber C, Dolle F, Pappata S, Planas AM, Tavitian B, Schafers M, Sorokin LM, Kuhlmann MT, Jacobs AH
* Multimodal imaging reveals temporal and spatial microglia and matrix metalloproteinase activity after experimental stroke (418 views)
J Cerebr Blood F Met (ISSN: 0271-678x), 2015 Nov; 35(11): 1711-1721.
Impact Factor: 4.929
View Export to BibTeX Export to EndNote
Nold-Petry CA, Rudloff I, Baumer Y, Ruvo M, Marasco D, Botti P, Farkas L, Cho SX, Zepp JA, Azam T, Dinkel H, Palmer BE, Boisvert WA, Cool CD, Taraseviciene-Stewart L, Heinhuis B, Joosten LA, Dinarello CA, Voelkel NF, Nold MF
* IL-32 Promotes Angiogenesis (1033 views)
J Immunol (ISSN: 0022-1767), 2014 Jan 15; 192(2): 589-602.
Impact Factor: 4.922
View Export to BibTeX Export to EndNote
Catanzaro V, Gringeri CV, Menchise V, Padovan S, Boffa C, Dastru W, Chaabane L, Digilio G, Aime S
* A R2p/R1p ratiometric procedure to assess matrix metalloproteinase-2 activity by magnetic resonance imaging (570 views)
Angew Chem Int Ed (ISSN: 1433-7851, 1521-3773electronic, 1433-7851linking), 2013 Apr 2; 52(14): 3926-3930.
Impact Factor: 11.336
View Export to BibTeX Export to EndNote
Gringeri CV, Menchise V, Rizzitelli S, Cittadino E, Catanzaro V, Dati G, Chaabane L, Digilio G, Aime S
* Novel Gd(III)-based probes for MR molecular imaging of matrix metalloproteinases (646 views)
Contrast Media Mol Imaging (ISSN: 1555-4309, 1555-4309linking), 2012 Mar; 7(2): 175-184.
Impact Factor: 2.872
View Export to BibTeX Export to EndNote
Del Vecchio S, Zannetti A, Iommelli F, Lettieri A, Brunetti A, Salvatore M
* Molecular imaging of tumor microenvironment (911 views)
Q J Nucl Med Mol Im (ISSN: 1824-4785, 1824-4478, 1824-4785linking), 2010 Jun; 54(3): 243-258.
Impact Factor: 1.724
View Export to BibTeX Export to EndNote
Del Vecchio S, Zannetti A, Iommelli F, Lettieri A, Brunetti A, Salvatore M
* Molecular imaging of tumor microenvironment: Challenges and perspectives (723 views)
Q J Nucl Med Mol Im (ISSN: 1824-4785, 1824-4478, 1824-4785linking), 2010 Jun; 54(3): 249-258.
Impact Factor: 1.724
View Export to BibTeX Export to EndNote
Migliore T, Parrella LS, Caputi A, Silvestri N, Romano R, Pace L, Imbriaco M, Losi MA, Betocchi S
* Pathogenesis of hypertrophic cardiomyopathy. Impact of growth factors on left ventricular anatomy (498 views)
Minerva Cardioangiol (ISSN: 0026-4725), 2008 Feb; 56(1): 13-20.
Impact Factor: 0.481
View Export to BibTeX Export to EndNote
Frasca F, Nucera C, Pellegriti G, Gangemi P, Attard M, Stella M, Loda M, Vella V, Giordano C, Trimarchi F, Mazzoni E, Belfiore A, Vigneri R
* BRAF(V600E) mutation and the biology of papillary thyroid cancer (900 views)
Endocr Relat Cancerendocrine Related Cancer (ISSN: 1351-0088), 2008; 15(1): 191-205.
Impact Factor: 5.236
View Export to BibTeX Export to EndNote
Grasso G, Fragai M, Rizzarelli E, Spoto G, Yeo KJ
* A new methodology for monitoring the activity of cdMMP-12 anchored and freeze-dried on Au (111) (533 views)
Journal Of The American Society For Mass Spectrometry (ISSN: 1044-0305), 2007 May; 18(5): 961-969.
Impact Factor: 3.664
View Export to BibTeX Export to EndNote
Grasso G, Fragai M, Rizzarelli E, Spoto G, Yeo KJ
* In situ AP/MALDI-MS characterization of anchored matrix metalloproteinases (652 views)
J Mass Spectrom (ISSN: 1076-5174, 1096-9888), 2006 Dec; 41(12): 1561-1569.
Impact Factor: 2.945
View Export to BibTeX Export to EndNote
Grasso G, D'Agata R, Rizzarelli E, Spoto G, D'Andrea LD, Pedone C, Picardi A, Romanelli A, Fragai M, Yeo KJ
* Activity of anchored human matrix metalloproteinase-1 catalytic domain on Au(111) surfaces monitored by ESI-MS (736 views)
J Mass Spectrom (ISSN: 1076-5174, 1096-9888), 2005 Dec; 40(12): 1565-1571.
Impact Factor: 3.574
View Export to BibTeX Export to EndNote
* Collagen degradation in tuberculosis pathogenesis: the biochemical consequences of hosting an undesired guest (574 views)
Biochem J (ISSN: 1470-8728electronic, 0264-6021linking, 0264-6021print), 2018 Oct 11; 475(19): 3123-3140.
Impact Factor: 4.897
View Export to BibTeX Export to EndNote
Zinnhardt B, Viel T, Wachsmuth L, Vrachimis A, Wagner S, Breyholz HJ, Faust A, Hermann S, Kopka K, Faber C, Dolle F, Pappata S, Planas AM, Tavitian B, Schafers M, Sorokin LM, Kuhlmann MT, Jacobs AH
* Multimodal imaging reveals temporal and spatial microglia and matrix metalloproteinase activity after experimental stroke (418 views)
J Cerebr Blood F Met (ISSN: 0271-678x), 2015 Nov; 35(11): 1711-1721.
Impact Factor: 4.929
View Export to BibTeX Export to EndNote
Nold-Petry CA, Rudloff I, Baumer Y, Ruvo M, Marasco D, Botti P, Farkas L, Cho SX, Zepp JA, Azam T, Dinkel H, Palmer BE, Boisvert WA, Cool CD, Taraseviciene-Stewart L, Heinhuis B, Joosten LA, Dinarello CA, Voelkel NF, Nold MF
* IL-32 Promotes Angiogenesis (1033 views)
J Immunol (ISSN: 0022-1767), 2014 Jan 15; 192(2): 589-602.
Impact Factor: 4.922
View Export to BibTeX Export to EndNote
Catanzaro V, Gringeri CV, Menchise V, Padovan S, Boffa C, Dastru W, Chaabane L, Digilio G, Aime S
* A R2p/R1p ratiometric procedure to assess matrix metalloproteinase-2 activity by magnetic resonance imaging (570 views)
Angew Chem Int Ed (ISSN: 1433-7851, 1521-3773electronic, 1433-7851linking), 2013 Apr 2; 52(14): 3926-3930.
Impact Factor: 11.336
View Export to BibTeX Export to EndNote
Gringeri CV, Menchise V, Rizzitelli S, Cittadino E, Catanzaro V, Dati G, Chaabane L, Digilio G, Aime S
* Novel Gd(III)-based probes for MR molecular imaging of matrix metalloproteinases (646 views)
Contrast Media Mol Imaging (ISSN: 1555-4309, 1555-4309linking), 2012 Mar; 7(2): 175-184.
Impact Factor: 2.872
View Export to BibTeX Export to EndNote
Del Vecchio S, Zannetti A, Iommelli F, Lettieri A, Brunetti A, Salvatore M
* Molecular imaging of tumor microenvironment (911 views)
Q J Nucl Med Mol Im (ISSN: 1824-4785, 1824-4478, 1824-4785linking), 2010 Jun; 54(3): 243-258.
Impact Factor: 1.724
View Export to BibTeX Export to EndNote
Del Vecchio S, Zannetti A, Iommelli F, Lettieri A, Brunetti A, Salvatore M
* Molecular imaging of tumor microenvironment: Challenges and perspectives (723 views)
Q J Nucl Med Mol Im (ISSN: 1824-4785, 1824-4478, 1824-4785linking), 2010 Jun; 54(3): 249-258.
Impact Factor: 1.724
View Export to BibTeX Export to EndNote
Migliore T, Parrella LS, Caputi A, Silvestri N, Romano R, Pace L, Imbriaco M, Losi MA, Betocchi S
* Pathogenesis of hypertrophic cardiomyopathy. Impact of growth factors on left ventricular anatomy (498 views)
Minerva Cardioangiol (ISSN: 0026-4725), 2008 Feb; 56(1): 13-20.
Impact Factor: 0.481
View Export to BibTeX Export to EndNote
Frasca F, Nucera C, Pellegriti G, Gangemi P, Attard M, Stella M, Loda M, Vella V, Giordano C, Trimarchi F, Mazzoni E, Belfiore A, Vigneri R
* BRAF(V600E) mutation and the biology of papillary thyroid cancer (900 views)
Endocr Relat Cancerendocrine Related Cancer (ISSN: 1351-0088), 2008; 15(1): 191-205.
Impact Factor: 5.236
View Export to BibTeX Export to EndNote
Grasso G, Fragai M, Rizzarelli E, Spoto G, Yeo KJ
* A new methodology for monitoring the activity of cdMMP-12 anchored and freeze-dried on Au (111) (533 views)
Journal Of The American Society For Mass Spectrometry (ISSN: 1044-0305), 2007 May; 18(5): 961-969.
Impact Factor: 3.664
View Export to BibTeX Export to EndNote
Grasso G, Fragai M, Rizzarelli E, Spoto G, Yeo KJ
* In situ AP/MALDI-MS characterization of anchored matrix metalloproteinases (652 views)
J Mass Spectrom (ISSN: 1076-5174, 1096-9888), 2006 Dec; 41(12): 1561-1569.
Impact Factor: 2.945
View Export to BibTeX Export to EndNote
Grasso G, D'Agata R, Rizzarelli E, Spoto G, D'Andrea LD, Pedone C, Picardi A, Romanelli A, Fragai M, Yeo KJ
* Activity of anchored human matrix metalloproteinase-1 catalytic domain on Au(111) surfaces monitored by ESI-MS (736 views)
J Mass Spectrom (ISSN: 1076-5174, 1096-9888), 2005 Dec; 40(12): 1565-1571.
Impact Factor: 3.574
View Export to BibTeX Export to EndNote
12 Records (12 excluding Abstracts).
Total impact factor: 48.304 (48.304 excluding Abstracts).
Total 5 year impact factor: 50.237 (50.237 excluding Abstracts).
Total impact factor: 48.304 (48.304 excluding Abstracts).
Total 5 year impact factor: 50.237 (50.237 excluding Abstracts).
523 views. Last view on Tuesday 10 December 2024, 10:33:23
ibb.cnr.it
