Resistance of decoy PNA-DNA chimeras to enzymatic degradation in cellular extracts and serum (372 views)
Oncology Research (ISSN: 0965-0407), 2003; 13(5): 279-287.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 1.794, 5-year impact factor: 1.193
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-0842305853&partnerID=40&md5=10517ce6415c4947d843c73d317532fd
Keywords: Decoy, Peptide Nucleic Acid (pna), Peptide Nucleic Acid (pna)-Dna Chimeras, Cell Extract, Endonuclease, Exonuclease, Immunoglobulin Enhancer Binding Protein, Liposome, Protein P50, Transcription Factor, Transcription Factor Decoy, Transcription Factor Sp1, Unclassified Drug, Exodeoxyribonuclease, Oligonucleotide, Phosphatase, Article, Binding Site, Cancer Invasion, Controlled Study, Dna Binding, Dna Flanking Region, Enzyme Activity, Enzyme Degradation, Gene Targeting, Human, Human Cell, In Vitro Study, In Vivo Study, Priority Journal, Protein Localization, Tumor Growth, Animal, Blood, Cattle, Cell Line, Chemistry, Dose Response, Enzyme Activation, Metabolism, Physiology, Dose-Response Relationship, Phosphoric Monoester Hydrolases,
Affiliations:
*** IBB - CNR ***
Dept. of Biochem. and Molec. Biology, Ferrara University, Ferrara, Italy
Inst. of Biostructure and Bioimaging, CNR, Napoli, Italy
Dept. Pharmaceut. Chem. and Technol., University of Perugia, Perugia, Italy
Biotechnology Center, Ferrara University, Ferrara, Italy
Dipto. di Biochim. e Biol. Molec., Via L. Borsari n.46, 44100 Ferrara, Italy
References:
Morishita, R., Sugimoto, T., Aoki, M., Kida, I., Tomita, N., Moriguchi, A., Maeda, K., Ogihara, T., In vivo transfection of cis element "decoy" against nuclear factor-kappaB binding site prevents myocardial infarction (1997) Nat. Med., 3, pp. 894-89
Tomita, N., Morishita, R., Tomita, S., Yamamoto, K., Aoki, M., Matsushita, H., Hayashi, S., Ogihara, T., Transcription factor decoy for nuclear factor-kappaB inhibits tumor necrosis factor-alpha-induced expression of interleukin-6 and intracellular adhesion molecule-1 in endothelial cells (1998) J. Hypertens., 16, pp. 993-1000
Hosoya, T., Takeuchi, H., Kanesaka, Y., Yamakawa, H., Miyano-Kurosaki, N., Takai, K., Yamamoto, N., Takaku, H., Sequence-specific inhibition of a transcription factor by circular dumbbell DNA oligonucleotides (1999) FEBS Lett., 461, pp. 136-140
Park, Y.G., Nesterova, M., Agrawal, S., Cho-Chung, Y.S., Dual blockade of cyclic AMP response element (CRE)- and AP-1-directed transcription by CRE-transcription factor decoy oligonucleotide gene-specific inhibition of tumor growth (1999) J. Biol. Chem., 274, pp. 1573-1580
Cho-Chung, Y.S., Park, Y.G., Nesterova, M., Lee, Y.N., Cho, Y.S., CRE-decoy oligonucleotide-inhibition of gene expression and tumor growth (2000) Mol. Cell. Biochem., 212, pp. 29-34
Ishibashi, H., Nakagawa, K., Onimaru, M., Castellanous, E.J., Kaneda, Y., Nakashima, Y., Shirasuna, K., Sueishi, K., Sp1 decoy transfected to carcinoma cells suppresses the expression of vascular endothelial growth factor, transforming growth factor beta1, and tissue factor and also cell growth and invasion activities (2000) Cancer Res., 60, pp. 6531-6536
Kawauchi, M., Suzuki, J., Morishita, R., Wada, Y., Izawa, A., Tomita, N., Amano, J., Isobe, M., Gene therapy for attenuating cardiac allograft arteriopathy using ex vivo E2F decoy transfection by HVJ-AVE-liposome method in mice and nonhuman primates (2000) Circ. Res., 87, pp. 1063-1068
Lee, Y.N., Park, Y.G., Choi, Y.H., Cho, Y.S., Cho-Chung, Y.S., CRE-transcription factor decoy oligonucleotide inhibition of MCF-7 breast cancer cells: Cross-talk with p53 signaling pathway (2000) Biochemistry, 39, pp. 4863-4868
Vos, I.H., Govers, R., Grone, H.J., Kleij, L., Schurink, M., De Wager, R.A., Goldschmeding, R., Robelink, T.J., NFkappaB decoy oligodeoxynucleotides reduce monocyte infiltration in renal allografts (2000) FASEB J., 14, pp. 815-822
Clusel, C., Meguenni, S., Elias, I., Vasseur, M., Blumenfeld, M., Inhibition of HSV-1 proliferation by decoy phosphodiester oligonucleotides containing ICP4 recognition sequences (1994) Gene Expr., 4, pp. 301-309
Morishita, R., Gibbons, G.H., Horiuchi, M., Ellison, K.E., Nakama, M., Zhang, L., Kaneda, Y., Dzan, V.J., A gene therapy strategy using a transcription factor decoy of the E2F binding site inhibits smooth muscle proliferation in vivo (1995) Proc. Natl. Acad. Sci. USA, 92, pp. 5855-5859
Kozlov, I.A., Kubareva, E.A., Ivanovskaya, M.G., Shabarova, Z.A., Design of new reagents on the base of DNA duplexes for irreversible inhibition of transcription factor NF-kappa B (1997) Antisense Nucleic Acid Drug Dev., 7, pp. 279-289
Lim, C.S., Jabrane-Ferrat, N., Fontes, J.D., Okamoto, H., Garovoy, M.R., Peterlin, B.M., Hunt, C.A., Sequence-independent inhibition of RNA transcription by DNA dumbbells and other decoys (1997) Nucleic Acids Res., 25, pp. 575-580
Yamashita, J., Yoshimasa, T., Arai, H., Hiraoka, J., Takaya, K., Miyamoto, Y., Ogawa, Y., Nakao, K., Identification of cis-elements of the human endothelin-A receptor gene and inhibition of the gene expression by the decoy strategy (1998) J. Biol. Chem., 273, pp. 15993-15999
Mischiati, C., Feriotto, G., Borgatti, M., Giacomini, P., Gambari, R., Characterization of a major histocompatibility complex class II X-box-binding protein enhancing tat-induced transcription directed by the human immunodeficiency virus type 1 long terminal repeat (2000) J. Virol., 74, pp. 8989-9001
Kawamura, I., Morishita, R., Tomita, N., Lacey, E., Aketa, M., Tsujimoto, S., Manda, T., Ogihara, T., Intratumoral injection of oligonucleotides to the NF kappa B binding site inhibits cachexia in a mouse tumor model (1999) Gene Ther., 6, pp. 91-97
Romano, M.F., Lamberti, A., Bisogni, R., Tassone, P., Pagnini, D., Storti, G., Del Vecchio, L., Venuta, S., Enhancement of cytosine arabinoside-induced apoptosis in human myeloblastic leukemia cells by NF-kappa B/Rel-specific decoy oligodeoxynucleotides (2000) Gene Ther., 7, pp. 1234-1237
Sharma, H.W., Narayanan, R., The NF-kappaB transcription factor in oncogenesis (1996) Anticancer Res., 16, pp. 589-596
Sharma, H.W., Perez, J.R., Higgins-Sochaski, K., Hsiao, R., Narayanan, R., Transcription factor decoy approach to decipher the role of NF-kappa B in oncogenesis (1996) Anticancer Res., 16, pp. 61-69
Cho-Chung, Y.S., Nesterova, M., Becker, K.G., Srivastava, R., Park, Y.G., Lee, Y.N., Cho, Y.S., Cheadle, C., Dissecting the circuitry of protein kinase A and cAMP signaling in cancer genesis: Antisense, microarray, gene overexpression, and transcription factor decoy (2002) Ann. NY Acad. Sci., 968, pp. 22-36
Piva, R., Gambari, R., Transcription factor decoy (TFD) in breast cancer research and treatment (2002) Technol. Cancer Res. Treat., 1, pp. 405-416
Nielsen, P.E., Egholm, M., Berg, R.H., Buchardt, O., Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide (1991) Science, 254, pp. 1497-1500
Egholm, M., Buchardt, O., Nielsen, P.E., Berg, R.H., Peptide nucleic acids (PNA): Oligonucleotide analogues with an achiral peptide backbone (1992) J. Am. Chem. Soc., 114, pp. 1895-1897
Demidov, V.V., Potaman, V.N., Frank-Kamenetskii, M.D., Egholm, M., Buchard, O., Sonnichsen, S.H., Nielsen, P.E., Stability of peptide nucleic acids in human serum and cellular extracts (1994) Biochem. Pharmacol., 48, pp. 1310-1313
Scarfí, S., Giovine, M., Gasparini, A., Damonte, G., Millo, E., Pozzolini, M., Benatti, U., Modified peptide nucleic acids are internalized in mouse macrophages RAW 264.7 and inhibit inducible nitric oxide synthase (1999) FEBS Lett., 451, pp. 264-268
Gambari, R., Peptide nucleic acids (PNAs): A tool for the development of gene expression modifiers (2001) Curr. Pharm. Des., 7, pp. 1839-1862
Mischiati, C., Borgatti, M., Bianchi, N., Rutigliano, C., Tomassetti, M., Feriotto, G., Gambari, R., Interaction of the human NF-kappaB p52 transcription factor with DNA-PNA hybrids mimicking the NF-kappaB binding sites of the human immunodeficiency virus type 1 promoter (1999) J. Biol. Chem., 274, pp. 33114-33122
Saviano, M., Romanelli, A., Bucci, E., Pedone, C., Mischiati, C., Bianchi, N., Feriotto, G., Gambari, R., Computational procedures to explain the different biological activity of DNA/DNA. DNA/PNA and PNA/PNA hybrid molecules mimicking NF-kappaB binding sites (2000) J. Biomol. Struct. Dyn., 18, pp. 353-362
Romanelli, A., Pedone, C., Saviano, M., Bianchi, N., Borgatti, M., Mischiati, C., Gambari, R., Molecular interactions between nuclear factor κB (NF-κB) transcription factors and a PNA-DNA chimera mimicking NF-κB binding sites (2001) Eur. J. Biochem., 268, pp. 1-11
Borgatti, M., Breda, L., Cortesi, R., Nastruzzi, C., Romanelli, A., Saviano, M., Bianchi, N., Gambari, R., Cationic liposomes as delivery systems for double-stranded peptide nucleic acid (PNA)-DNA chimeras exhibiting decoy activity against nuclear factor-κB transcription factors (2002) Biochem. Pharmacol., 64, pp. 609-616
Vinayak, R., Van Der Laan, A.C., Brill, R., Otteson, K., Andrus, A., Kuyl-Yeheskiely, E., Van Boom, J.H., Automated chemical synthesis of PNA and PNA-DNA chimera on a nucleic acid synthesizer (1997) Nucleosides Nucleotides, 16, pp. 1653-1656
Nastruzzi, C., Cortesi, R., Esposito, E., Gambari, R., Borgatti, M., Bianchi, N., Feriotto, G., Mischiati, C., Liposomes as carriers for DNA-PNA hybrids (2000) J. Controlled Release, 68, pp. 237-249
Szklarczyk, A., Kaczmarek, L., Antisense oligodeoxyribonucleotides: Stability and distribution after intracerebral injection into rat brain (1995) J. Neurosci. Methods, 60, pp. 181-187
Ueno, Y., Kumagai, I., Haginoya, N., Matsuda, A., Effects of 5-(N-aminohexyl)carbamoyl-2′-deoxyuridine on endonuclease stability and the ability of oligodeoxynucleotide to activate RNase H (1997) Nucleic Acids Res., 25, pp. 3777-3782
Uhlmann, E., Ryte, A., Peyman, A., Studies on the mechanism of stabilization of partially phosphorothioated oligonucleotides against nucleolytic degradation (1997) Antisense Nucleic Acid Drug Dev., 7, pp. 345-350
Piva, R., Lambertini, E., Penolazzi, L., Facciolo, M.C., Lodi, A., Aguiari, G., Nastruzzi, C., Del Senno, L., In vitro stability of polymerase chain reaction-generated DNA fragments in serum and cell extracts (1998) Biochem. Pharmacol., 56, pp. 703-708
Park, Y. G., Nesterova, M., Agrawal, S., Cho-Chung, Y. S., Dual blockade of cyclic AMP response element (CRE) - and AP-1-directed transcription by CRE-transcription factor decoy oligonucleotide gene-specific inhibition of tumor growth (1999) J. Biol. Chem., 274, pp. 1573-1580
Cho-Chung, Y. S., Park, Y. G., Nesterova, M., Lee, Y. N., Cho, Y. S., CRE-decoy oligonucleotide-inhibition of gene expression and tumor growth (2000) Mol. Cell. Biochem., 212, pp. 29-34
Lee, Y. N., Park, Y. G., Choi, Y. H., Cho, Y. S., Cho-Chung, Y. S., CRE-transcription factor decoy oligonucleotide inhibition of MCF-7 breast cancer cells: Cross-talk with p53 signaling pathway (2000) Biochemistry, 39, pp. 4863-4868
Vos, I. H., Govers, R., Grone, H. J., Kleij, L., Schurink, M., De Wager, R. A., Goldschmeding, R., Robelink, T. J., NFkappaB decoy oligodeoxynucleotides reduce monocyte infiltration in renal allografts (2000) FASEB J., 14, pp. 815-822
Kozlov, I. A., Kubareva, E. A., Ivanovskaya, M. G., Shabarova, Z. A., Design of new reagents on the base of DNA duplexes for irreversible inhibition of transcription factor NF-kappa B (1997) Antisense Nucleic Acid Drug Dev., 7, pp. 279-289
Lim, C. S., Jabrane-Ferrat, N., Fontes, J. D., Okamoto, H., Garovoy, M. R., Peterlin, B. M., Hunt, C. A., Sequence-independent inhibition of RNA transcription by DNA dumbbells and other decoys (1997) Nucleic Acids Res., 25, pp. 575-580
Romano, M. F., Lamberti, A., Bisogni, R., Tassone, P., Pagnini, D., Storti, G., Del Vecchio, L., Venuta, S., Enhancement of cytosine arabinoside-induced apoptosis in human myeloblastic leukemia cells by NF-kappa B/Rel-specific decoy oligodeoxynucleotides (2000) Gene Ther., 7, pp. 1234-1237
Sharma, H. W., Narayanan, R., The NF-kappaB transcription factor in oncogenesis (1996) Anticancer Res., 16, pp. 589-596
Sharma, H. W., Perez, J. R., Higgins-Sochaski, K., Hsiao, R., Narayanan, R., Transcription factor decoy approach to decipher the role of NF-kappa B in oncogenesis (1996) Anticancer Res., 16, pp. 61-69
Cho-Chung, Y. S., Nesterova, M., Becker, K. G., Srivastava, R., Park, Y. G., Lee, Y. N., Cho, Y. S., Cheadle, C., Dissecting the circuitry of protein kinase A and cAMP signaling in cancer genesis: Antisense, microarray, gene overexpression, and transcription factor decoy (2002) Ann. NY Acad. Sci., 968, pp. 22-36
Nielsen, P. E., Egholm, M., Berg, R. H., Buchardt, O., Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide (1991) Science, 254, pp. 1497-1500
Demidov, V. V., Potaman, V. N., Frank-Kamenetskii, M. D., Egholm, M., Buchard, O., Sonnichsen, S. H., Nielsen, P. E., Stability of peptide nucleic acids in human serum and cellular extracts (1994) Biochem. Pharmacol., 48, pp. 1310-1313
Scarf, S., Giovine, M., Gasparini, A., Damonte, G., Millo, E., Pozzolini, M., Benatti, U., Modified peptide nucleic acids are internalized in mouse macrophages RAW 264. 7 and inhibit inducible nitric oxide synthase (1999) FEBS Lett., 451, pp. 264-268
Oncology Research (ISSN: 0965-0407), 2003; 13(5): 279-287.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 1.794, 5-year impact factor: 1.193
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-0842305853&partnerID=40&md5=10517ce6415c4947d843c73d317532fd
Keywords: Decoy, Peptide Nucleic Acid (pna), Peptide Nucleic Acid (pna)-Dna Chimeras, Cell Extract, Endonuclease, Exonuclease, Immunoglobulin Enhancer Binding Protein, Liposome, Protein P50, Transcription Factor, Transcription Factor Decoy, Transcription Factor Sp1, Unclassified Drug, Exodeoxyribonuclease, Oligonucleotide, Phosphatase, Article, Binding Site, Cancer Invasion, Controlled Study, Dna Binding, Dna Flanking Region, Enzyme Activity, Enzyme Degradation, Gene Targeting, Human, Human Cell, In Vitro Study, In Vivo Study, Priority Journal, Protein Localization, Tumor Growth, Animal, Blood, Cattle, Cell Line, Chemistry, Dose Response, Enzyme Activation, Metabolism, Physiology, Dose-Response Relationship, Phosphoric Monoester Hydrolases,
Affiliations:
*** IBB - CNR ***
Dept. of Biochem. and Molec. Biology, Ferrara University, Ferrara, Italy
Inst. of Biostructure and Bioimaging, CNR, Napoli, Italy
Dept. Pharmaceut. Chem. and Technol., University of Perugia, Perugia, Italy
Biotechnology Center, Ferrara University, Ferrara, Italy
Dipto. di Biochim. e Biol. Molec., Via L. Borsari n.46, 44100 Ferrara, Italy
References:
Morishita, R., Sugimoto, T., Aoki, M., Kida, I., Tomita, N., Moriguchi, A., Maeda, K., Ogihara, T., In vivo transfection of cis element "decoy" against nuclear factor-kappaB binding site prevents myocardial infarction (1997) Nat. Med., 3, pp. 894-89
Tomita, N., Morishita, R., Tomita, S., Yamamoto, K., Aoki, M., Matsushita, H., Hayashi, S., Ogihara, T., Transcription factor decoy for nuclear factor-kappaB inhibits tumor necrosis factor-alpha-induced expression of interleukin-6 and intracellular adhesion molecule-1 in endothelial cells (1998) J. Hypertens., 16, pp. 993-1000
Hosoya, T., Takeuchi, H., Kanesaka, Y., Yamakawa, H., Miyano-Kurosaki, N., Takai, K., Yamamoto, N., Takaku, H., Sequence-specific inhibition of a transcription factor by circular dumbbell DNA oligonucleotides (1999) FEBS Lett., 461, pp. 136-140
Park, Y.G., Nesterova, M., Agrawal, S., Cho-Chung, Y.S., Dual blockade of cyclic AMP response element (CRE)- and AP-1-directed transcription by CRE-transcription factor decoy oligonucleotide gene-specific inhibition of tumor growth (1999) J. Biol. Chem., 274, pp. 1573-1580
Cho-Chung, Y.S., Park, Y.G., Nesterova, M., Lee, Y.N., Cho, Y.S., CRE-decoy oligonucleotide-inhibition of gene expression and tumor growth (2000) Mol. Cell. Biochem., 212, pp. 29-34
Ishibashi, H., Nakagawa, K., Onimaru, M., Castellanous, E.J., Kaneda, Y., Nakashima, Y., Shirasuna, K., Sueishi, K., Sp1 decoy transfected to carcinoma cells suppresses the expression of vascular endothelial growth factor, transforming growth factor beta1, and tissue factor and also cell growth and invasion activities (2000) Cancer Res., 60, pp. 6531-6536
Kawauchi, M., Suzuki, J., Morishita, R., Wada, Y., Izawa, A., Tomita, N., Amano, J., Isobe, M., Gene therapy for attenuating cardiac allograft arteriopathy using ex vivo E2F decoy transfection by HVJ-AVE-liposome method in mice and nonhuman primates (2000) Circ. Res., 87, pp. 1063-1068
Lee, Y.N., Park, Y.G., Choi, Y.H., Cho, Y.S., Cho-Chung, Y.S., CRE-transcription factor decoy oligonucleotide inhibition of MCF-7 breast cancer cells: Cross-talk with p53 signaling pathway (2000) Biochemistry, 39, pp. 4863-4868
Vos, I.H., Govers, R., Grone, H.J., Kleij, L., Schurink, M., De Wager, R.A., Goldschmeding, R., Robelink, T.J., NFkappaB decoy oligodeoxynucleotides reduce monocyte infiltration in renal allografts (2000) FASEB J., 14, pp. 815-822
Clusel, C., Meguenni, S., Elias, I., Vasseur, M., Blumenfeld, M., Inhibition of HSV-1 proliferation by decoy phosphodiester oligonucleotides containing ICP4 recognition sequences (1994) Gene Expr., 4, pp. 301-309
Morishita, R., Gibbons, G.H., Horiuchi, M., Ellison, K.E., Nakama, M., Zhang, L., Kaneda, Y., Dzan, V.J., A gene therapy strategy using a transcription factor decoy of the E2F binding site inhibits smooth muscle proliferation in vivo (1995) Proc. Natl. Acad. Sci. USA, 92, pp. 5855-5859
Kozlov, I.A., Kubareva, E.A., Ivanovskaya, M.G., Shabarova, Z.A., Design of new reagents on the base of DNA duplexes for irreversible inhibition of transcription factor NF-kappa B (1997) Antisense Nucleic Acid Drug Dev., 7, pp. 279-289
Lim, C.S., Jabrane-Ferrat, N., Fontes, J.D., Okamoto, H., Garovoy, M.R., Peterlin, B.M., Hunt, C.A., Sequence-independent inhibition of RNA transcription by DNA dumbbells and other decoys (1997) Nucleic Acids Res., 25, pp. 575-580
Yamashita, J., Yoshimasa, T., Arai, H., Hiraoka, J., Takaya, K., Miyamoto, Y., Ogawa, Y., Nakao, K., Identification of cis-elements of the human endothelin-A receptor gene and inhibition of the gene expression by the decoy strategy (1998) J. Biol. Chem., 273, pp. 15993-15999
Mischiati, C., Feriotto, G., Borgatti, M., Giacomini, P., Gambari, R., Characterization of a major histocompatibility complex class II X-box-binding protein enhancing tat-induced transcription directed by the human immunodeficiency virus type 1 long terminal repeat (2000) J. Virol., 74, pp. 8989-9001
Kawamura, I., Morishita, R., Tomita, N., Lacey, E., Aketa, M., Tsujimoto, S., Manda, T., Ogihara, T., Intratumoral injection of oligonucleotides to the NF kappa B binding site inhibits cachexia in a mouse tumor model (1999) Gene Ther., 6, pp. 91-97
Romano, M.F., Lamberti, A., Bisogni, R., Tassone, P., Pagnini, D., Storti, G., Del Vecchio, L., Venuta, S., Enhancement of cytosine arabinoside-induced apoptosis in human myeloblastic leukemia cells by NF-kappa B/Rel-specific decoy oligodeoxynucleotides (2000) Gene Ther., 7, pp. 1234-1237
Sharma, H.W., Narayanan, R., The NF-kappaB transcription factor in oncogenesis (1996) Anticancer Res., 16, pp. 589-596
Sharma, H.W., Perez, J.R., Higgins-Sochaski, K., Hsiao, R., Narayanan, R., Transcription factor decoy approach to decipher the role of NF-kappa B in oncogenesis (1996) Anticancer Res., 16, pp. 61-69
Cho-Chung, Y.S., Nesterova, M., Becker, K.G., Srivastava, R., Park, Y.G., Lee, Y.N., Cho, Y.S., Cheadle, C., Dissecting the circuitry of protein kinase A and cAMP signaling in cancer genesis: Antisense, microarray, gene overexpression, and transcription factor decoy (2002) Ann. NY Acad. Sci., 968, pp. 22-36
Piva, R., Gambari, R., Transcription factor decoy (TFD) in breast cancer research and treatment (2002) Technol. Cancer Res. Treat., 1, pp. 405-416
Nielsen, P.E., Egholm, M., Berg, R.H., Buchardt, O., Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide (1991) Science, 254, pp. 1497-1500
Egholm, M., Buchardt, O., Nielsen, P.E., Berg, R.H., Peptide nucleic acids (PNA): Oligonucleotide analogues with an achiral peptide backbone (1992) J. Am. Chem. Soc., 114, pp. 1895-1897
Demidov, V.V., Potaman, V.N., Frank-Kamenetskii, M.D., Egholm, M., Buchard, O., Sonnichsen, S.H., Nielsen, P.E., Stability of peptide nucleic acids in human serum and cellular extracts (1994) Biochem. Pharmacol., 48, pp. 1310-1313
Scarfí, S., Giovine, M., Gasparini, A., Damonte, G., Millo, E., Pozzolini, M., Benatti, U., Modified peptide nucleic acids are internalized in mouse macrophages RAW 264.7 and inhibit inducible nitric oxide synthase (1999) FEBS Lett., 451, pp. 264-268
Gambari, R., Peptide nucleic acids (PNAs): A tool for the development of gene expression modifiers (2001) Curr. Pharm. Des., 7, pp. 1839-1862
Mischiati, C., Borgatti, M., Bianchi, N., Rutigliano, C., Tomassetti, M., Feriotto, G., Gambari, R., Interaction of the human NF-kappaB p52 transcription factor with DNA-PNA hybrids mimicking the NF-kappaB binding sites of the human immunodeficiency virus type 1 promoter (1999) J. Biol. Chem., 274, pp. 33114-33122
Saviano, M., Romanelli, A., Bucci, E., Pedone, C., Mischiati, C., Bianchi, N., Feriotto, G., Gambari, R., Computational procedures to explain the different biological activity of DNA/DNA. DNA/PNA and PNA/PNA hybrid molecules mimicking NF-kappaB binding sites (2000) J. Biomol. Struct. Dyn., 18, pp. 353-362
Romanelli, A., Pedone, C., Saviano, M., Bianchi, N., Borgatti, M., Mischiati, C., Gambari, R., Molecular interactions between nuclear factor κB (NF-κB) transcription factors and a PNA-DNA chimera mimicking NF-κB binding sites (2001) Eur. J. Biochem., 268, pp. 1-11
Borgatti, M., Breda, L., Cortesi, R., Nastruzzi, C., Romanelli, A., Saviano, M., Bianchi, N., Gambari, R., Cationic liposomes as delivery systems for double-stranded peptide nucleic acid (PNA)-DNA chimeras exhibiting decoy activity against nuclear factor-κB transcription factors (2002) Biochem. Pharmacol., 64, pp. 609-616
Vinayak, R., Van Der Laan, A.C., Brill, R., Otteson, K., Andrus, A., Kuyl-Yeheskiely, E., Van Boom, J.H., Automated chemical synthesis of PNA and PNA-DNA chimera on a nucleic acid synthesizer (1997) Nucleosides Nucleotides, 16, pp. 1653-1656
Nastruzzi, C., Cortesi, R., Esposito, E., Gambari, R., Borgatti, M., Bianchi, N., Feriotto, G., Mischiati, C., Liposomes as carriers for DNA-PNA hybrids (2000) J. Controlled Release, 68, pp. 237-249
Szklarczyk, A., Kaczmarek, L., Antisense oligodeoxyribonucleotides: Stability and distribution after intracerebral injection into rat brain (1995) J. Neurosci. Methods, 60, pp. 181-187
Ueno, Y., Kumagai, I., Haginoya, N., Matsuda, A., Effects of 5-(N-aminohexyl)carbamoyl-2′-deoxyuridine on endonuclease stability and the ability of oligodeoxynucleotide to activate RNase H (1997) Nucleic Acids Res., 25, pp. 3777-3782
Uhlmann, E., Ryte, A., Peyman, A., Studies on the mechanism of stabilization of partially phosphorothioated oligonucleotides against nucleolytic degradation (1997) Antisense Nucleic Acid Drug Dev., 7, pp. 345-350
Piva, R., Lambertini, E., Penolazzi, L., Facciolo, M.C., Lodi, A., Aguiari, G., Nastruzzi, C., Del Senno, L., In vitro stability of polymerase chain reaction-generated DNA fragments in serum and cell extracts (1998) Biochem. Pharmacol., 56, pp. 703-708
Park, Y. G., Nesterova, M., Agrawal, S., Cho-Chung, Y. S., Dual blockade of cyclic AMP response element (CRE) - and AP-1-directed transcription by CRE-transcription factor decoy oligonucleotide gene-specific inhibition of tumor growth (1999) J. Biol. Chem., 274, pp. 1573-1580
Cho-Chung, Y. S., Park, Y. G., Nesterova, M., Lee, Y. N., Cho, Y. S., CRE-decoy oligonucleotide-inhibition of gene expression and tumor growth (2000) Mol. Cell. Biochem., 212, pp. 29-34
Lee, Y. N., Park, Y. G., Choi, Y. H., Cho, Y. S., Cho-Chung, Y. S., CRE-transcription factor decoy oligonucleotide inhibition of MCF-7 breast cancer cells: Cross-talk with p53 signaling pathway (2000) Biochemistry, 39, pp. 4863-4868
Vos, I. H., Govers, R., Grone, H. J., Kleij, L., Schurink, M., De Wager, R. A., Goldschmeding, R., Robelink, T. J., NFkappaB decoy oligodeoxynucleotides reduce monocyte infiltration in renal allografts (2000) FASEB J., 14, pp. 815-822
Kozlov, I. A., Kubareva, E. A., Ivanovskaya, M. G., Shabarova, Z. A., Design of new reagents on the base of DNA duplexes for irreversible inhibition of transcription factor NF-kappa B (1997) Antisense Nucleic Acid Drug Dev., 7, pp. 279-289
Lim, C. S., Jabrane-Ferrat, N., Fontes, J. D., Okamoto, H., Garovoy, M. R., Peterlin, B. M., Hunt, C. A., Sequence-independent inhibition of RNA transcription by DNA dumbbells and other decoys (1997) Nucleic Acids Res., 25, pp. 575-580
Romano, M. F., Lamberti, A., Bisogni, R., Tassone, P., Pagnini, D., Storti, G., Del Vecchio, L., Venuta, S., Enhancement of cytosine arabinoside-induced apoptosis in human myeloblastic leukemia cells by NF-kappa B/Rel-specific decoy oligodeoxynucleotides (2000) Gene Ther., 7, pp. 1234-1237
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Resistance of decoy PNA-DNA chimeras to enzymatic degradation in cellular extracts and serum
Double-stranded molecules based on peptide nucleic acids (PNAs)-DNA chimeras carrying binding sites for known transcription factors could be of great interest in decoy pharmacotherapy of neoplastic diseases. For instance, decoy molecules recognizing Sp1 and NF-kappaB transcription factors were found to inhibit tumor cell growth and invasion activity. In this respect, we have recently found that double-stranded PNA-DNA chimeras carrying NF-kappaB binding sites inhibit the binding of NF-kappaB p52 and p50 transcription factors to target DNA molecules. In this article we determined the resistance of double-stranded decoy molecules based on PNA-DNA chimeras to exonucleases (both 3'-->5' and 5'-->3' exonucleases), endonucleases, and 5'-phosphatases. In addition, we performed experiments aimed at determining the resistance of these molecules in cellular extracts and serum. Finally, we used liposomes as protective agents in experimental conditions in which the decoy molecules employed were found to be unstable (high concentrations of enzymes, cellular extracts, or serum). The results obtained demonstrated that decoy molecules based on PNA-DNA chimeras are more resistant than DNA-based decoys to exo- and endonucleases, serum, and cellular extracts. In addition, the resistance of DNA/PNA hybrids in the presence of high concentrations of serum and cellular extracts was increased after complexation to cationic liposomes, due to the fact that double-stranded PNA-DNA-PNA chimeras bind to these delivery systems. The results obtained in the present study support the proposal of molecules based on PNA-DNA chimeras for an efficient decoy treatment of tumor cells both in vitro and in vivo.
Double-stranded molecules based on peptide nucleic acids (PNAs)-DNA chimeras carrying binding sites for known transcription factors could be of great interest in decoy pharmacotherapy of neoplastic diseases. For instance, decoy molecules recognizing Sp1 and NF-kappaB transcription factors were found to inhibit tumor cell growth and invasion activity. In this respect, we have recently found that double-stranded PNA-DNA chimeras carrying NF-kappaB binding sites inhibit the binding of NF-kappaB p52 and p50 transcription factors to target DNA molecules. In this article we determined the resistance of double-stranded decoy molecules based on PNA-DNA chimeras to exonucleases (both 3'-->5' and 5'-->3' exonucleases), endonucleases, and 5'-phosphatases. In addition, we performed experiments aimed at determining the resistance of these molecules in cellular extracts and serum. Finally, we used liposomes as protective agents in experimental conditions in which the decoy molecules employed were found to be unstable (high concentrations of enzymes, cellular extracts, or serum). The results obtained demonstrated that decoy molecules based on PNA-DNA chimeras are more resistant than DNA-based decoys to exo- and endonucleases, serum, and cellular extracts. In addition, the resistance of DNA/PNA hybrids in the presence of high concentrations of serum and cellular extracts was increased after complexation to cationic liposomes, due to the fact that double-stranded PNA-DNA-PNA chimeras bind to these delivery systems. The results obtained in the present study support the proposal of molecules based on PNA-DNA chimeras for an efficient decoy treatment of tumor cells both in vitro and in vivo.
Resistance of decoy PNA-DNA chimeras to enzymatic degradation in cellular extracts and serum
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Resistance of decoy PNA-DNA chimeras to enzymatic degradation in cellular extracts and serum
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Moccia M, Mercurio FA, Langella E, Piacenti V, Leone M, Adamo MFA, Saviano M
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Poletto M, Vascotto C, Scognamiglio PL, Lirussi L, Marasco D, Tell G
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Lirussi L, Antoniali G, Vascotto C, D'Ambrosio C, Poletto M, Romanello M, Marasco D, Leone M, Quadrifoglio F, Bhakat KK, Scaloni A, Tell G
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Poletto M, Di Loreto C, Marasco D, Poletto E, Puglisi F, Damante G, Tell G
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Fantini D, Vascotto C, Marasco D, D'Ambrosio C, Romanello M, Vitagliano L, Pedone C, Poletto M, Cesaratto L, Quadrifoglio F, Scaloni A, Radicella JP, Tell G
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D'Agata R, Breveglieri G, Zanoli L, Borgatti M, Spoto G, Gambari R
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Mai A, Rotili D, Tarantino D, Ornaghi P, Tosi F, Vicidomini C, Sbardella G, Nebbioso A, Miceli M, Altucci L, Filetici P
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Copani A, Hoozemans JJM, Caraci F, Calafiore M, Van Haastert ES, Veerhuis R, Rozemuller AJM, Aronica E, Sortino MA, Nicoletti F
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Mischiati C, Sereni A, Finotti A, Breda L, Cortesi R, Nastruzzi C, Romanelli A, Saviano M, Bianchi N, Pedone C, Borgatti M, Gambari R
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Menchise V, De Simone G, Tedeschi T, Corradini R, Sforza S, Marchelli R, Capasso D, Saviano M, Pedone C
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Impact Factor: 10.272
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Borgatti M, Lampronti I, Romanelli A, Pedone C, Saviano M, Bianchi N, Mischiati C, Gambari R
* Transcription factor decoy molecules based on a peptide nucleic acid (PNA)-DNA chimera mimicking Sp1 binding sites (538 views)
Jbc Papers (ISSN: 0021-9258, 1083-351x, 0021-9258linking), 2003 Feb 28; 278(9): 7500-7509.
Impact Factor: 6.482
View Export to BibTeX Export to EndNote
Borgatti M, Breda L, Cortesi R, Nastruzzi C, Romanelli A, Saviano M, Bianchi N, Mischiati C, Pedone C, Gambari R
* Cationic Liposomes As Delivery Systems For Double-Stranded Pna-Dna Chimeras Exhibiting Decoy Activity Against Nf-Kappab Transcription Factors (416 views)
Biochem Pharmacol Biochemical Pharmacology (ISSN: 1873-2968, 0006-2952), 2002 Sep 15; 64(4): 609-616.
Impact Factor: 3.542
View Export to BibTeX Export to EndNote
* Structural Insights on Tiny Peptide Nucleic Acid (PNA) Analogues of miRNA-34a: An in silico and Experimental Integrated Approach (74 views)
Front Chem (ISSN: 2296-2646linking), 2020 Nov 23; 8: 568575-568575.
Impact Factor: 3.693
View Export to BibTeX Export to EndNote
Piacenti V, Langella E, Autiero I, Nolan JC, Piskareva O, Adamo MFA, Saviano M, Moccia M
* A combined experimental and computational study on peptide nucleic acid (PNA) analogues of tumor suppressive miRNA-34a (222 views)
Bioorg Chem (ISSN: 0045-2068linking, 1090-2120electronic), 2019 Oct; 91: 103165-103165.
Impact Factor: 3.9
View Export to BibTeX Export to EndNote
Sandomenico A, Focà A, Sanguigno L, Caporale A, Focà G, Pignalosa A, Corvino G, Caragnano A, Beltrami AP, Antoniali G, Tell G, Leonardi A, Ruvo M
* Monoclonal antibodies against pools of mono- and polyacetylated peptides selectively recognize acetylated lysines within the context of the original antigen (391 views) (PDF 219 views)
Mabs-Austin (ISSN: 1942-0862), 2016 Aug; 8: 1575-1589.
Impact Factor: 4.881
View Export to BibTeX Export to EndNote
Poletto M, Vascotto C, Scognamiglio PL, Lirussi L, Marasco D, Tell G
* Role Of The Unstructured N-Terminal Domain Of The Human Apurinic/Apyrimidinic Endonuclease 1 (hape1) In The Modulation Of Its Interaction With Nucleic Acids And Nucleophosmin (npm1) (495 views)
Biochem J (ISSN: 0264-6021, 1470-8728electronic, 0264-6021linking), 2013; 452(3): 545-557.
Impact Factor: 4.779
View Export to BibTeX Export to EndNote
Lirussi L, Antoniali G, Vascotto C, D'Ambrosio C, Poletto M, Romanello M, Marasco D, Leone M, Quadrifoglio F, Bhakat KK, Scaloni A, Tell G
* Nucleolar accumulation of APE1 depends on charged lysine residues that undergo acetylation upon genotoxic stress and modulate its BER activity in cells (675 views)
Mol Biol Cell (ISSN: 1059-1524), 2012 Oct 15; 23(20): 4079-4096.
Impact Factor: 4.803
View Export to BibTeX Export to EndNote
Poletto M, Di Loreto C, Marasco D, Poletto E, Puglisi F, Damante G, Tell G
* Acetylation on critical lysine residues of Apurinic/apyrimidinic endonuclease 1 (APE1) in triple negative breast cancers (482 views)
Biochemical And Biophysical Research Communications (ISSN: 0006-291x), 2012 Jul 20; 424(1): 34-39.
Impact Factor: 2.406
View Export to BibTeX Export to EndNote
Fantini D, Vascotto C, Marasco D, D'Ambrosio C, Romanello M, Vitagliano L, Pedone C, Poletto M, Cesaratto L, Quadrifoglio F, Scaloni A, Radicella JP, Tell G
* Critical lysine residues within the overlooked N-terminal domain of human APE1 regulate its biological functions (414 views)
Nucleic Acids Res (ISSN: 1362-4962, 0305-1048, 1362-4962electronic), 2010 Dec; 38(22): 8239-8256.
Impact Factor: 7.836
View Export to BibTeX Export to EndNote
D'Agata R, Breveglieri G, Zanoli L, Borgatti M, Spoto G, Gambari R
* Surface Plasmon Resonance Imaging (SPR-I), peptide nucleic acid (PNA) probes and nanoparticle-enhancement for PCR-free ultrasensitive detection of beta-thalassemia mutations in human genomic DNA (298 views)
International Journal Of Molecular Medicine (ISSN: 1107-3756), 2010; 26: N/D-N/D.
Impact Factor: 1.814
View Export to BibTeX Export to EndNote
Mai A, Rotili D, Tarantino D, Ornaghi P, Tosi F, Vicidomini C, Sbardella G, Nebbioso A, Miceli M, Altucci L, Filetici P
* Small-molecule inhibitors of histone acetyltransferase activity: Identification and biological properties (523 views)
J Med Chem (ISSN: 0022-2623, 1520-4804, 0022-2623print), 2006 Nov 16; 49(23): 6897-6907.
Impact Factor: 5.115
View Export to BibTeX Export to EndNote
Copani A, Hoozemans JJM, Caraci F, Calafiore M, Van Haastert ES, Veerhuis R, Rozemuller AJM, Aronica E, Sortino MA, Nicoletti F
* Dna Polymerase-Beta Is Expressed Early In Neurons Of Alzheimer'S Disease Brain And Is Loaded Into Dna Replication Forks In Neurons Challenged With Beta-Amyloid (491 views)
J Neurosci (ISSN: 0270-6474, 0361-803x, 1529-2401), 2006; 26(43): 10949-10957.
Impact Factor: 2.117
View Export to BibTeX Export to EndNote
Mischiati C, Sereni A, Finotti A, Breda L, Cortesi R, Nastruzzi C, Romanelli A, Saviano M, Bianchi N, Pedone C, Borgatti M, Gambari R
* Complexation to cationic microspheres of double-stranded peptide nucleic acid-DNA chimeras exhibiting decoy activity (361 views)
Journal Of Biomedical Science (ISSN: 1021-7770), 2004 Sep; 11(5): 697-704.
Impact Factor: 1.567
View Export to BibTeX Export to EndNote
Menchise V, De Simone G, Tedeschi T, Corradini R, Sforza S, Marchelli R, Capasso D, Saviano M, Pedone C
* Insights into peptide nucleic acid (PNA) structural features: The crystal structure of a D-lysine-based chiral PNA-DNA duplex (519 views)
P Natl Acad Sci Usa (ISSN: 0027-8424, 1091-6490, 0027-8424print), 2003 Oct 14; 100(21): 12021-12026.
Impact Factor: 10.272
View Export to BibTeX Export to EndNote
Borgatti M, Lampronti I, Romanelli A, Pedone C, Saviano M, Bianchi N, Mischiati C, Gambari R
* Transcription factor decoy molecules based on a peptide nucleic acid (PNA)-DNA chimera mimicking Sp1 binding sites (538 views)
Jbc Papers (ISSN: 0021-9258, 1083-351x, 0021-9258linking), 2003 Feb 28; 278(9): 7500-7509.
Impact Factor: 6.482
View Export to BibTeX Export to EndNote
Borgatti M, Breda L, Cortesi R, Nastruzzi C, Romanelli A, Saviano M, Bianchi N, Mischiati C, Pedone C, Gambari R
* Cationic Liposomes As Delivery Systems For Double-Stranded Pna-Dna Chimeras Exhibiting Decoy Activity Against Nf-Kappab Transcription Factors (416 views)
Biochem Pharmacol Biochemical Pharmacology (ISSN: 1873-2968, 0006-2952), 2002 Sep 15; 64(4): 609-616.
Impact Factor: 3.542
View Export to BibTeX Export to EndNote
14 Records (13 excluding Abstracts).
Total impact factor: 63.207 (61.393 excluding Abstracts).
Total 5 year impact factor: 66.097 (64.278 excluding Abstracts).
Total impact factor: 63.207 (61.393 excluding Abstracts).
Total 5 year impact factor: 66.097 (64.278 excluding Abstracts).
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