Cosolute effect on crystallization of two dinucleotide complexes of bovine seminal ribonuclease from concentrated salt solutions(293 views) Sica F, Adinolfi S, Vitagliano L, Zagari A, Capasso S, Mazzarella L
Affiliations: Dipartimento di Chimica, Univ.' Federico II Napoli Centro S., CNR, Via Mezzocannone 4, I-80134 Napoli, Italy
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Cosolute effect on crystallization of two dinucleotide complexes of bovine seminal ribonuclease from concentrated salt solutions
Two complexes of bovine seminal ribonuclease with dinudeotides, uridylyl(2′-5′)adenosine (UpA) and 2′-deoxycytidylyl(3′-5′)-2′-deoxyadenosine (d(CpA)), were crystallized under unusual conditions involving a liquid-liquid phase separation. This phenomenon was induced by adding small aliquots of organic cosolutes to highly concentrated ammonium sulfate solutions. The liquid-liquid interface acts as a source of nucleation centers for growth of large crystals. Among the cosolutes tested in these salt-mediated crystallizations, 2-methyl-2,4-pentanediol was found to be the most efficient.
Cosolute effect on crystallization of two dinucleotide complexes of bovine seminal ribonuclease from concentrated salt solutions
No results.
Cosolute effect on crystallization of two dinucleotide complexes of bovine seminal ribonuclease from concentrated salt solutions