Peptide nucleic acid molecular beacons for the detection of PCR amplicons in droplet-based microfluidic devices (663 views)
Anal Bioanal Chem (ISSN: 1618-2642), 2013 Jan; 405(2-3): 615-624.
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Paper type: Journal Article,
Impact factor: 3.578, 5-year impact factor: 3.744
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84873719566&partnerID=40&md5=46179cfcd18a7c4aa29f525ddbd5f1a5
Keywords: Dna Detection, Droplet Microfluidics, Molecular Beacon, Peptide Nucleic Acids, Bioelectric Phenomena, Biomolecules, Dna Sequences, Fluorescence Microscopy, Polymerase Chain Reaction, Plant Dna, Single Stranded Dna, Article, Chemistry, Classification, Equipment, Evaluation, Genetics, Methodology, Microfluidic Analysis, Olive Tree, Single Nucleotide Polymorphism, Soybean, Single-Stranded, Microfluidic Analytical Techniques, Glycine Max, Olea Europaea,
Affiliations:
*** IBB - CNR ***
Scuola Superiore di Catania, C/o Dipartimento di Scienze Chimiche, Università di Catania, Viale Andrea Doria 6, Catania 95125, Italy
Dipartimento di Chimica Organica e Industriale, Università di Parma, V.le G. P. Usberti 17/a, Parma 43100, Italy
Istituto Biostrutture e Bioimmagini, CNR, Viale A. Doria 6, Catania 95125, Italy
Center for Molecular Medicine, Austrian Academy of Sciences, Lazarettgasse 14, Vienna 1090, Austria
References:
Not available.
Anal Bioanal Chem (ISSN: 1618-2642), 2013 Jan; 405(2-3): 615-624.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 3.578, 5-year impact factor: 3.744
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84873719566&partnerID=40&md5=46179cfcd18a7c4aa29f525ddbd5f1a5
Keywords: Dna Detection, Droplet Microfluidics, Molecular Beacon, Peptide Nucleic Acids, Bioelectric Phenomena, Biomolecules, Dna Sequences, Fluorescence Microscopy, Polymerase Chain Reaction, Plant Dna, Single Stranded Dna, Article, Chemistry, Classification, Equipment, Evaluation, Genetics, Methodology, Microfluidic Analysis, Olive Tree, Single Nucleotide Polymorphism, Soybean, Single-Stranded, Microfluidic Analytical Techniques, Glycine Max, Olea Europaea,
Affiliations:
*** IBB - CNR ***
Scuola Superiore di Catania, C/o Dipartimento di Scienze Chimiche, Università di Catania, Viale Andrea Doria 6, Catania 95125, Italy
Dipartimento di Chimica Organica e Industriale, Università di Parma, V.le G. P. Usberti 17/a, Parma 43100, Italy
Istituto Biostrutture e Bioimmagini, CNR, Viale A. Doria 6, Catania 95125, Italy
Center for Molecular Medicine, Austrian Academy of Sciences, Lazarettgasse 14, Vienna 1090, Austria
References:
Not available.
Peptide nucleic acid molecular beacons for the detection of PCR amplicons in droplet-based microfluidic devices
The use of droplet-based microfluidics and peptide nucleic acid molecular beacons for the detection of polymerase chain reaction (PCR)-amplified DNA sequences within nanoliter-sized droplets is described in this work. The nanomolar-attomolar detection capabilities of the method were preliminarily tested by targeting two different single-stranded DNA sequences from the genetically modified Roundup Ready soybean and the Olea europaea genomes and detecting the fluorescence generated by peptide nucleic acid molecular beacons with fluorescence microscopy. Furthermore, the detection of 10 nM solutions of PCR amplicon of DNA extracted from leaves of O. europaea L. encapsulated in nanoliter-sized droplets was performed to demonstrate that peptide nucleic acid molecular beacons can discriminate O. europaea L. cultivar species carrying different single-nucleotide polymorphisms.
The use of droplet-based microfluidics and peptide nucleic acid molecular beacons for the detection of polymerase chain reaction (PCR)-amplified DNA sequences within nanoliter-sized droplets is described in this work. The nanomolar-attomolar detection capabilities of the method were preliminarily tested by targeting two different single-stranded DNA sequences from the genetically modified Roundup Ready soybean and the Olea europaea genomes and detecting the fluorescence generated by peptide nucleic acid molecular beacons with fluorescence microscopy. Furthermore, the detection of 10 nM solutions of PCR amplicon of DNA extracted from leaves of O. europaea L. encapsulated in nanoliter-sized droplets was performed to demonstrate that peptide nucleic acid molecular beacons can discriminate O. europaea L. cultivar species carrying different single-nucleotide polymorphisms.
Peptide nucleic acid molecular beacons for the detection of PCR amplicons in droplet-based microfluidic devices
| ▼ Biomolecular NMR: structural studies of proteins and peptides and drug discovery applications |
Peptide nucleic acid molecular beacons for the detection of PCR amplicons in droplet-based microfluidic devices
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Impact Factor: 4.493
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Impact Factor: 3.578
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View Export to BibTeX Export to EndNote
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Impact Factor: 3.914
View Export to BibTeX Export to EndNote
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Impact Factor: 0
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Impact Factor: 3.659
View Export to BibTeX Export to EndNote
Roviello GN, Musumeci D, Bucci EM, Pedone C
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Impact Factor: 3.35
View Export to BibTeX Export to EndNote
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Impact Factor: 4.106
View Export to BibTeX Export to EndNote
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Impact Factor: 0
View Export to BibTeX Export to EndNote
D'Agata R, Corradini R, Ferretti C, Zanoli L, Gatti M, Marchelli R, Spoto G
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Impact Factor: 5.361
View Export to BibTeX Export to EndNote
Roviello GN, Gröschel S, Pedone C, Diederichsen U
* Synthesis of novel MMT/acyl-protected nucleo alanine monomers for the preparation of DNA/alanyl-PNA chimeras (1437 views)
Amino Acids (ISSN: 1438-2199, 0939-4451, 1438-2199electronic), 2010 May; 38(5): 1301-1309.
Impact Factor: 4.106
View Export to BibTeX Export to EndNote
Bonomo RP, Di Natale G, Rizzarelli E, Tabbì G, Vagliasindi LI
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Impact Factor: 2.908
View Export to BibTeX Export to EndNote
Roviello GN, Musumeci D, Bucci EM, Castiglione M, Cesarani A, Pedone C, Piccialli G
* Evidences for complex formation between l-dabPNA and aegPNA (609 views)
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Impact Factor: 2.531
View Export to BibTeX Export to EndNote
D'Agata R, Corradini R, Grasso G, Marchelli R, Spoto G
* Ultrasensitive detection of DNA by PNA and nanoparticle-enhanced surface plasmon resonance imaging (745 views)
Chembiochem (ISSN: 1439-4227, 1439-7633, 1439-7633electronic), 2008 Sep 1; 9(13): 2067-2070.
Impact Factor: 3.322
View Export to BibTeX Export to EndNote
Breveglieri G, Pensato S, Salvatori F, Saviano M, Pedone C, Romanelli A, Gambari R
* PNA-mediated alteration of mRNA splicing (562 views)
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Impact Factor: 0.1
View Export to BibTeX Export to EndNote
Marchelli R, Saviano M, Licandro E, Spoto G, Borgatti M, Gambari R
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Impact Factor: 0.1
View Export to BibTeX Export to EndNote
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Minerva Biotecnol (ISSN: 1120-4826), 2008; 20(4): 165-174.
Impact Factor: 0.1
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Moccia M, Musumeci D, Valente M, Roviello GN, Sapio R, Pedone C, Bucci EM
* Thermodynamic studies on PNA and PNA/DNA dendrimer formation (552 views)
Nucleosides Nucleotides Nucleic Acids (ISSN: 1525-7770), 2007; 26(8-9): 1173-1176.
Impact Factor: 0.723
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Roviello GN, Moccia M, Sapio R, Valente M, Bucci EM, Castiglione M, Pedone C, Perretta G, Benedetti E, Musumeci D
* Synthesis, Characterization And Hybridization Studies Of New Nucleo-Gamma-Peptides Based On Diaminobutyric Acid (798 views)
J Pept Sci (ISSN: 1075-2617, 1099-1387, 1075-2617print), 2006 Dec; 12(12): 829-835.
Impact Factor: 1.801
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Borgatti M, Boyd DD, Lampronti I, Bianchi N, Fabbri E, Saviano M, Romanelli A, Pedone C, Gambari R
* Decoy molecules based on PNA-DNA chimeras and targeting Sp1 transcription factors inhibit the activity of urokinase-type plasminogen activator receptor (uPAR) promoter (808 views)
Oncology Research (ISSN: 0965-0407), 2005; 15(7-8): 373-383.
Impact Factor: 1.844
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Borgatti M, Finotti A, Romanelli A, Saviano M, Bianchi N, Lampronti I, Lambertini E, Penolazzi L, Nastruzzi C, Mischiati C, Piva R, Pedone C, Gambari R
* Peptide nucleic acids (PNA)-DNA chimeras targeting transcription factors as a tool to modify gene expression (809 views)
Curr Drug Targets (ISSN: 1389-4501), 2004 Nov; 5(8): 735-744.
Impact Factor: 4.104
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Cortesi R, Mischiati C, Borgatti M, Breda L, Romanelli A, Saviano M, Pedone C, Gambari R, Nastruzzi C
* Formulations for natural and peptide nucleic acids based on cationic polymeric submicron particles (624 views)
The Aaps Journal (ISSN: 1522-1059, 1550-7416), 2004 Jan 20; 6(1): N/D-N/D.
Impact Factor: 1.938
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Musumeci D, Roviello GN, Valente M, Sapio R, Pedone C, Bucci EM
* New synthesis of PNA-3′DNA linker monomers, useful building blocks to obtain PNA/DNA chimeras (655 views)
Biopolymers, 2004; 76(6): 535-542.
Impact Factor: 2.248
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Gambari R, Borgatti M, Romanelli A, Capasso D, Saviano M, Bianchi N, Lampronti I, Mischiati C, Pedone C
* Transcription factor decoy (TFD) activity of peptide nucleic acids (PNA)-DNA chimeras (511 views)
International Journal Of Molecular Medicine (ISSN: 1107-3756), 2003; 12(Supplement1): N/D-N/D.
Impact Factor: 1.94
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Romanelli A, Pedone C, Saviano M, Bianchi N, Borgatti M, Mischiati C, Gambari R
* Molecular interactions between nuclear factor κB (NF-κB) transcription factors and a PNA-DNA chimera mimicking NF-κB binding sites (914 views)
Eur J Biochem (ISSN: 0014-2956, 0014-2956print, 0014-2956linking), 2001 Dec; 268(23): 6066-6075.
Impact Factor: 2.849
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Romanelli A, Pedone C, Saviano M, Bianchi N, Borgatti M, Mischiati C, Gambari R
* Molecular interactions with nuclear factor kappaB (NF-kappaB) transcription factors of a PNA-DNA chimera mimicking NF-kappaB binding sites (665 views)
Eur J Biochem (ISSN: 0014-2956print, 0014-2956linking), 2001 Dec; 268(23): 6066-6075.
Impact Factor: 4.779
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34 Records (32 excluding Abstracts).
Total impact factor: 103.468 (99.59 excluding Abstracts).
Total 5 year impact factor: 104.722 (97.85 excluding Abstracts).
Total impact factor: 103.468 (99.59 excluding Abstracts).
Total 5 year impact factor: 104.722 (97.85 excluding Abstracts).
663 views. Last view on Monday 17 March 2025, 21:16:31
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