Keywords: Biomedical Applications, Diagnostic Applications, Direct Detection, Dna Amplification, Genomic Dna, Healthy Individuals, Point Mutations, Surface Plasmon Resonance Imaging, Ultrasensitive, Ultrasensitive Detection, Polymerase Chain Reaction, Gold, Metal Nanoparticle, Beta Thalassemia, Blood, Chemistry, Genetics, Human, Sensitivity And Specificity, Surface Property, Beta-Thalassemia, Surface Properties,
Affiliations: *** IBB - CNR ***
Dipartimento di Scienze Chimiche, Università di Catania, Viale Andrea Doria 6, I-95125 Catania, Italy
Centro di Biotecnologie, Università di Ferrara, via Fossato di Mortara 64b, 44121 Ferrara, Italy
Scuola Superiore di Catania, C/o Dipartimento di Scienze Chimiche, Università di Catania, Viale Andrea Doria 6, Catania, Italy
Istituto Biostrutture e Bioimmagini, CNR, Viale A. Doria 6, Catania, Italy
Dipartimento di Biochimica e Biologia Molecolare, Università di Ferrara, via Fossato di Mortara 74, 44121 Ferrara, Italy
References: Not available.
Direct Detection of Point Mutations in Nonamplified Human Genomic DNA
Ultrasensitive detection protocols not requiring polymerase chain reaction (PCR)-mediated target DNA amplification are expected to significantly improve our possibilities in several research and diagnostic applications for which minute cell quantities are available. For this reason we have tested a nanoparticle-enhanced surface plasmon resonance imaging (SPRI) sensing strategy to detect point mutations in nonamplified genomic DNA. We have used genomic DNAs, not subject to costly, time-consuming, and prone to contamination PCR-based amplification procedures, obtained from both healthy individuals and homozygous or heterozygous patients affected by beta-thalassemia, in order to demonstrate the specificity and the sensitivity of the described sensing strategy. The assay we describe is ultrasensitive and convenient. Attomolar concentrations of target genomic DNA are detected, DNAs from healthy individuals and homozygous or heterozygous patients affected by beta-thalassemia are discriminated, and only simple manipulations of the genetic samples are required before the analysis. The proposed ultrasensitive detection of DNA point mutations involved in genomic disorders possibly represents an important advantage in several biomedical applications.
Direct Detection of Point Mutations in Nonamplified Human Genomic DNA
Malvindi MA, Greco A, Conversano F, Figuerola A, Corti M, Bonora M, Lascialfari A, Doumari HA, Moscardini M, Cingolani R, Gigli G, Casciaro S, Pellegrino T, Ragusa A * MR Contrast Agents(479 views) Small Animal Imaging, 2011 Jul 8; 21(13): 2548-2555. Impact Factor:1.784 ViewExport to BibTeXExport to EndNote