Urotensin-II receptor is over-expressed in colon cancer cell lines and in colon carcinoma in humans(444 views) Federico A, Zappavigna S, Romano M, Grieco P, Luce A, Marra M, Gravina AG, Stiuso P, D'Armiento FP, Vitale G, Tuccillo C, Novellino E, Loguercio C, Caraglia M
Gastroenterology Unit, Department of Clinical and Experimental Medicine and Surgery, Second University of Naples, Naples, Italy
Department of Biochemistry, Biophysics and General Pathology, Second University of Naples, Naples, Italy
Department of Pharmacy, University of Naples Federico II, Naples, Italy
Department of Biomorphological and Functional Sciences, University Federico II, Naples, Italy
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Ames, R. S., Sarau, H. M., Chambers, J. K., Willette, R. N., Aiyar, N. V., Romanic, A. M., Human urotensin-II is a potent vasoconstrictor and agonist for the orphan receptor GPR14 (1999) Nature, 401, pp. 282-286
Beauvillain, J. C., Conlon, J. M., Bern, H. A., Vaudry, H., Cloning of the cDNA encoding the urotensin II precursor in frog and human reveals intense expression of the urotensin II gene in motoneurons of the spinal cord (1998) Proc Natl Acad Sci USA, 95, pp. 15803-15808
Elshourbagy, N. A., Douglas, S. A., Shabon, U., Harrison, S., Duddy, G., Sechler, J. L., Molecular and pharmacological characterization of genes encoding urotensin II-related peptides and their cognate G-protein coupled receptors from the mouse and monkey (2002) Br J Pharmacol, 136, pp. 9-22
Kinney, W. A., Almond, H. R., Qi, J., Smith, C. E., Santulli, R. J., de Garavilla, L., Structure-function analysis of urotensin II and its use in the construction of a ligand-receptor working model (2002) Angew Chem Int Ed Engl, 41, pp. 2940-2944
Coy, D. H., Rossowski, W. J., Cheng, B. L., Taylor, J. E., Structural requirements at the N-terminus of urotensin II octapeptides (2002) Peptides, 23, pp. 2259-2264
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Bosman, F. T., (2010) WHO Classification of Tumours of the Digestive System, , Organization WH and Cancer, IAFRO. Lyon: International Agency for Research on Cancer
Wu, Y. Q., Song, Z., Zhou, C. H., Xing, S. H., Pei, D. S., Zheng, J. N., Expression of urotensin II and its receptor in human lung adenocarcinoma A549 cells and the effect of urotensin II on lung adenocarcinoma growth in vitro and in vivo (2010) Oncol Rep, 24, pp. 1179-1184
Kristof, A. S., You, Z., Han, Y. S., Giaid, A., Protein expression of urotensin II, urotensin-related peptide and their receptor in the lungs of patients with lymphangioleiomyomatosis (2010) Peptides, 31, pp. 1511-1516
Zeng, Z. P., Liu, G. Q., Li, H. Z., Fan, X. R., Liu, D. M., Tong, A. L., The effects of urotensin-II on proliferation of pheochromocytoma cells and mRNA expression of urotensin-II and its receptor in pheochromocytoma tissues (2006) Ann N Y Acad Sci, 1073, pp. 284-289
Urotensin-II receptor is over-expressed in colon cancer cell lines and in colon carcinoma in humans
BackgroundUrotensin (U)-II receptor (UTR) has been previously reported to be over-expressed in a number of tumours. Whether UTR-related pathway plays a role in colon carcinogenesis is unknown. MethodsWe evaluated UTR protein and mRNA expression in human epithelial colon cancer cell lines and in normal colon tissue, adenomatous polyps and colon cancer. U-II protein expression was assessed in cancer cell lines. Moreover, we evaluated the effects of U-II(4-11) (an UTR agonist), antagonists and knockdown of UTR protein expression through a specific shRNA, on proliferation, invasion and motility of human colon cancer cells. ResultsCancer cell lines expressed U-II protein and UTR protein and mRNA. By immunohistochemistry, UTR was expressed in 5-30% of epithelial cells in 45 normal controls, in 30-48% in 21 adenomatous polyps and in 65-90% in 48 colon adenocarcinomas. UTR mRNA expression was increased by threefold in adenomatous polyps and eightfold in colon cancer, compared with normal colon. U-II(4-11) induced a 20-40% increase in cell growth while the blockade of the receptor with specific antagonists caused growth inhibition of 20-40%. Moreover, the knock down of UTR with a shRNA or the inhibition of UTR with the antagonist urantide induced an approximately 50% inhibition of both motility and invasion. ConclusionsUTR appears to be involved in the regulation of colon cancer cell invasion and motility. These data suggest that UTR-related pathway may play a role in colon carcinogenesis and that UTR may function as a target for therapeutic intervention in colon cancer.
Urotensin-II receptor is over-expressed in colon cancer cell lines and in colon carcinoma in humans
No results.
Urotensin-II receptor is over-expressed in colon cancer cell lines and in colon carcinoma in humans
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