CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF AN NAD+-DEPENDENT ALCOHOL-DEHYDROGENASE FROM THE EXTREME THERMOPHILIC ARCHAEBACTERIUM SULFOLOBUS-SOLFATARICUS
CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF AN NAD+-DEPENDENT ALCOHOL-DEHYDROGENASE FROM THE EXTREME THERMOPHILIC ARCHAEBACTERIUM SULFOLOBUS-SOLFATARICUS(486 views) PEARL L, DEMASI D, HEMMINGS A, SICA F, MAZZARELLA L, RAIA C, DAURIA S, ROSSI M
Keywords: Alcohol Dehydrogenase, Archaebacteria, Extreme Thermophile, Protein Thermostability, Sulfolobus Solfataricus, Nicotinamide Adenine Dinucleotide, Article, Crystal Structure, Nonhuman, Priority Journal, Protein Stability, Thermophilic Bacterium, X Ray Analysis, X-Ray Diffraction,
Affiliations: Biomol. Structure and Modelling Unit, Dept. of Biochem. and Molec. Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom
Dipartimento di Chimica, Univ. Studi di Napoli Federico II, Via Mezzocannone 4, 80134 Napoli, Italy
Department of Crystallography, Birkbeck College, Malet Street, London WC1E 7HX, United Kingdom
Ist. Biochim. Proteine Enzimologia, Consiglio Nazionale delle Ricerche, Via Marconi 12, I-80125 Napoli, Italy
References: Not available.
CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF AN NAD+-DEPENDENT ALCOHOL-DEHYDROGENASE FROM THE EXTREME THERMOPHILIC ARCHAEBACTERIUM SULFOLOBUS-SOLFATARICUS
An NAD+-dependent alcohol dehydrogenase from the extreme thermophilic archaebacterium Sulfolobus solfataricus has been crystallized in the holo-enzyme and apo-enzyme forms. Crystals of the holo-enzyme grow from 2-methyl-2,4-pentanediol at pH 8.4 with the addition of NADH and at pH 7.0 with the addition of NADH and dimethyl sulphoxide. Crystals of the apo-enzyme grow at pH 6.3 from a mixture of polyethylene glycol 4000 and propan-2-ol. The holo-enzyme crystallizes in C2 with a dimer in the asymmetric unit, however the crystals are twinned and unsuitable for data collection. The apo-enzyme crystallizes in I4122 (a =126.82 Å, b = 118.95 Å) with a monomer in the asymmetric unit, and the single crystals diffract to 2.8 Å.
CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF AN NAD+-DEPENDENT ALCOHOL-DEHYDROGENASE FROM THE EXTREME THERMOPHILIC ARCHAEBACTERIUM SULFOLOBUS-SOLFATARICUS
No results.
CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF AN NAD+-DEPENDENT ALCOHOL-DEHYDROGENASE FROM THE EXTREME THERMOPHILIC ARCHAEBACTERIUM SULFOLOBUS-SOLFATARICUS