Expression, purification and partial characterization of the Krüppel-associated box (KRAB) from the human ZNF2 protein (362 views)
Protein Pept Lett (ISSN: 0929-8665, 1875-5305), 2005; 12(6): 527-532.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 0.84, 5-year impact factor: 1.493
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-23644442274&partnerID=40&md5=99d6624f6b29f3f0245d9c0b0fabda65
Keywords: Fusion Protein Expression, Krab Domain, Transcriptional Repression, Zinc Finger, Dna Binding Protein, Recombinant Protein, Znf2 Protein, Human, Amino Acid Sequence, Article, Biosynthesis, Chemistry, Dna Sequence, Escherichia Coli, Gel Chromatography, Gene Expression, Genetics, High Performance Liquid Chromatography, Isolation And Purification, Molecular Genetics, Molecular Weight, Polyacrylamide Gel Electrophoresis, Protein Tertiary Structure, Sequence Alignment, High Pressure Liquid, Dna-Binding Proteins, Molecular Sequence Data, Protein Structure, Sequence Analysis,
Affiliations:
*** IBB - CNR ***
Istituto di Genetica e Biofisica A. Buzzati Traverso, Consiglio Nazionale Delle Ricerche, Italy
Istituto di Biochimica Delle Proteine, Consiglio Nazionale Delle Ricerche, Italy
Istituto di Biostrutture e Bioimmagini, Consiglio Nazionale Delle Ricerche, Italy
Dipartimento di Biochimica e Biofisica F. Cedrangolo, Seconda Università di Napoli, Italy
References:
Bellefroid, E.J., Poncelet, D.A., Lecocq, P.J., Relevant, O., Martial, J.A., (1991) Proc. Natl. Acad. Sci. USA, 88, pp. 3608-361
Rosati, M., Marino, M., Franzè, A., Tramontano, A., Grimaldi, G., (1991) Nucleic Acids Res., 19, pp. 5661-5667
Bellefroid, E.J., Marine, J.C., Ried, T., Lecocq, P.J., Riviere, M., Amemiya, C., Poncelet, D.A., Martial, J.A., (1993) EMBO J., 12, pp. 1363-1374
Rosati, M., Franzè, A., Matarazzo, M.R., Grimaldi, G., (1999) Cytogenet. Cell. Genet., 85, pp. 291-296
Margolin, J.F., Friedman, J.R., Meyer, W.K.-H., Vissing, H., Thiesen, H.-J., Rauscher III, F.J., (1994) Proc. Natl. Acad. Sci. USA, 91, pp. 4509-4513
Senatore, B., Cafieri, A., Di Marino, I., Rosati, M., Di Nocera, P.P., Grimaldi, G., (1999) Gene, 234, pp. 381-394
Abrink, M., Ortiz, J.A., Mark, C., Sanchez, C., Looman, C., Hellman, L., Chambon, P., Losson, R., (2001) Proc. Natl. Acad. Sci. USA, 98, pp. 1422-1426
Friedman, J.R., Fredericks, W.J., Jensen, D.E., Speicher, D.W., Huang, X.P., Neilson, E.G., Rauscher III, F.J., (1996) Genes and Devel., 10, pp. 2067-2078
Peng, H., Begg, G.E., Harper, S.L., Friedman, J.R., Speicher, D.W., Rauscher III, F.J., (2000) J. Biol. Chem., 275, pp. 18000-18010
Schultz, D.C., Ayyanathan, K., Negorev, D., Maul, G.G., Rauscher III, F.J., (2002) Genes & Development, 16, pp. 919-932
Collins, T., Stone, J.R., Williams, A.J., (2001) Mol. Cell. Biol., 21, pp. 3609-3615
Li, Z., Wang, D., Na, X., Schoen, S.R., Messing, E.M., Wu, G., (2003) EMBO J., 22, pp. 1857-1867
Hennemann, H., Vassen, L., Geisen, C., Eilers, M., Möröy, T., (2003) J. Biol. Chem., 278, pp. 28799-28811
Fredericks, W.J., Herlyn, M., Friedman, J.R., Rauscher III, F.J., (2000) Mol. Cell. Biol., 20, pp. 5019-5031
De Haan, G., Chusacultanachai, S., Mao, C., Katzenellenbogen, B.S., Shapiro, D.J., (2000) J. Biol. Chem., 275, pp. 13493-13501
Reynolds, L., Ullman, C., Moore, M., Isalan, M., West, M.J., Clapham, P., Klug, A., Choo, Y., (2003) Proc. Natl. Acad. Sci. USA, 100, pp. 1615-1620
Papworth, M., Moore, M., Isalan, M., Minczuk, M., Choo, Y., Klug, A., (2003) Proc. Natl. Acad. Sci. USA, 100, pp. 1621-1626
Bellefroid, E. J., Poncelet, D. A., Lecocq, P. J., Relevant, O., Martial, J. A., (1991) Proc. Natl. Acad. Sci. USA, 88, pp. 3608-361
Rosati, M., Franz, A., Matarazzo, M. R., Grimaldi, G., (1999) Cytogenet. Cell. Genet., 85, pp. 291-296
Margolin, J. F., Friedman, J. R., Meyer, W. K. -H., Vissing, H., Thiesen, H. -J., Rauscher III, F. J., (1994) Proc. Natl. Acad. Sci. USA, 91, pp. 4509-4513
Friedman, J. R., Fredericks, W. J., Jensen, D. E., Speicher, D. W., Huang, X. P., Neilson, E. G., Rauscher III, F. J., (1996) Genes and Devel., 10, pp. 2067-2078
Peng, H., Begg, G. E., Harper, S. L., Friedman, J. R., Speicher, D. W., Rauscher III, F. J., (2000) J. Biol. Chem., 275, pp. 18000-18010
Schultz, D. C., Ayyanathan, K., Negorev, D., Maul, G. G., Rauscher III, F. J., (2002) Genes & Development, 16, pp. 919-932
Fredericks, W. J., Herlyn, M., Friedman, J. R., Rauscher III, F. J., (2000) Mol. Cell. Biol., 20, pp. 5019-5031
Protein Pept Lett (ISSN: 0929-8665, 1875-5305), 2005; 12(6): 527-532.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 0.84, 5-year impact factor: 1.493
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-23644442274&partnerID=40&md5=99d6624f6b29f3f0245d9c0b0fabda65
Keywords: Fusion Protein Expression, Krab Domain, Transcriptional Repression, Zinc Finger, Dna Binding Protein, Recombinant Protein, Znf2 Protein, Human, Amino Acid Sequence, Article, Biosynthesis, Chemistry, Dna Sequence, Escherichia Coli, Gel Chromatography, Gene Expression, Genetics, High Performance Liquid Chromatography, Isolation And Purification, Molecular Genetics, Molecular Weight, Polyacrylamide Gel Electrophoresis, Protein Tertiary Structure, Sequence Alignment, High Pressure Liquid, Dna-Binding Proteins, Molecular Sequence Data, Protein Structure, Sequence Analysis,
Affiliations:
*** IBB - CNR ***
Istituto di Genetica e Biofisica A. Buzzati Traverso, Consiglio Nazionale Delle Ricerche, Italy
Istituto di Biochimica Delle Proteine, Consiglio Nazionale Delle Ricerche, Italy
Istituto di Biostrutture e Bioimmagini, Consiglio Nazionale Delle Ricerche, Italy
Dipartimento di Biochimica e Biofisica F. Cedrangolo, Seconda Università di Napoli, Italy
References:
Bellefroid, E.J., Poncelet, D.A., Lecocq, P.J., Relevant, O., Martial, J.A., (1991) Proc. Natl. Acad. Sci. USA, 88, pp. 3608-361
Rosati, M., Marino, M., Franzè, A., Tramontano, A., Grimaldi, G., (1991) Nucleic Acids Res., 19, pp. 5661-5667
Bellefroid, E.J., Marine, J.C., Ried, T., Lecocq, P.J., Riviere, M., Amemiya, C., Poncelet, D.A., Martial, J.A., (1993) EMBO J., 12, pp. 1363-1374
Rosati, M., Franzè, A., Matarazzo, M.R., Grimaldi, G., (1999) Cytogenet. Cell. Genet., 85, pp. 291-296
Margolin, J.F., Friedman, J.R., Meyer, W.K.-H., Vissing, H., Thiesen, H.-J., Rauscher III, F.J., (1994) Proc. Natl. Acad. Sci. USA, 91, pp. 4509-4513
Senatore, B., Cafieri, A., Di Marino, I., Rosati, M., Di Nocera, P.P., Grimaldi, G., (1999) Gene, 234, pp. 381-394
Abrink, M., Ortiz, J.A., Mark, C., Sanchez, C., Looman, C., Hellman, L., Chambon, P., Losson, R., (2001) Proc. Natl. Acad. Sci. USA, 98, pp. 1422-1426
Friedman, J.R., Fredericks, W.J., Jensen, D.E., Speicher, D.W., Huang, X.P., Neilson, E.G., Rauscher III, F.J., (1996) Genes and Devel., 10, pp. 2067-2078
Peng, H., Begg, G.E., Harper, S.L., Friedman, J.R., Speicher, D.W., Rauscher III, F.J., (2000) J. Biol. Chem., 275, pp. 18000-18010
Schultz, D.C., Ayyanathan, K., Negorev, D., Maul, G.G., Rauscher III, F.J., (2002) Genes & Development, 16, pp. 919-932
Collins, T., Stone, J.R., Williams, A.J., (2001) Mol. Cell. Biol., 21, pp. 3609-3615
Li, Z., Wang, D., Na, X., Schoen, S.R., Messing, E.M., Wu, G., (2003) EMBO J., 22, pp. 1857-1867
Hennemann, H., Vassen, L., Geisen, C., Eilers, M., Möröy, T., (2003) J. Biol. Chem., 278, pp. 28799-28811
Fredericks, W.J., Herlyn, M., Friedman, J.R., Rauscher III, F.J., (2000) Mol. Cell. Biol., 20, pp. 5019-5031
De Haan, G., Chusacultanachai, S., Mao, C., Katzenellenbogen, B.S., Shapiro, D.J., (2000) J. Biol. Chem., 275, pp. 13493-13501
Reynolds, L., Ullman, C., Moore, M., Isalan, M., West, M.J., Clapham, P., Klug, A., Choo, Y., (2003) Proc. Natl. Acad. Sci. USA, 100, pp. 1615-1620
Papworth, M., Moore, M., Isalan, M., Minczuk, M., Choo, Y., Klug, A., (2003) Proc. Natl. Acad. Sci. USA, 100, pp. 1621-1626
Bellefroid, E. J., Poncelet, D. A., Lecocq, P. J., Relevant, O., Martial, J. A., (1991) Proc. Natl. Acad. Sci. USA, 88, pp. 3608-361
Rosati, M., Franz, A., Matarazzo, M. R., Grimaldi, G., (1999) Cytogenet. Cell. Genet., 85, pp. 291-296
Margolin, J. F., Friedman, J. R., Meyer, W. K. -H., Vissing, H., Thiesen, H. -J., Rauscher III, F. J., (1994) Proc. Natl. Acad. Sci. USA, 91, pp. 4509-4513
Friedman, J. R., Fredericks, W. J., Jensen, D. E., Speicher, D. W., Huang, X. P., Neilson, E. G., Rauscher III, F. J., (1996) Genes and Devel., 10, pp. 2067-2078
Peng, H., Begg, G. E., Harper, S. L., Friedman, J. R., Speicher, D. W., Rauscher III, F. J., (2000) J. Biol. Chem., 275, pp. 18000-18010
Schultz, D. C., Ayyanathan, K., Negorev, D., Maul, G. G., Rauscher III, F. J., (2002) Genes & Development, 16, pp. 919-932
Fredericks, W. J., Herlyn, M., Friedman, J. R., Rauscher III, F. J., (2000) Mol. Cell. Biol., 20, pp. 5019-5031
Expression, purification and partial characterization of the Krüppel-associated box (KRAB) from the human ZNF2 protein
The Krüppel-associated box (KRAB) domain is a potent transcription repression bipartite domain, shared by over 400 zinc finger proteins in humans, involved in the regulation of many functions. KRAB domains are both physically and functionally bipartite (A and B modules). The lone KRAB-A and composite KRAB-AB domains from the human ZNF2 protein were over-expressed as recombinant proteins in E. coli, isolated and purified to homogeneity to investigate their structure to function relationship. © 2005 Bentham Science Publishers Ltd.
The Krüppel-associated box (KRAB) domain is a potent transcription repression bipartite domain, shared by over 400 zinc finger proteins in humans, involved in the regulation of many functions. KRAB domains are both physically and functionally bipartite (A and B modules). The lone KRAB-A and composite KRAB-AB domains from the human ZNF2 protein were over-expressed as recombinant proteins in E. coli, isolated and purified to homogeneity to investigate their structure to function relationship. © 2005 Bentham Science Publishers Ltd.
Expression, purification and partial characterization of the Krüppel-associated box (KRAB) from the human ZNF2 protein
| ▼ Structural characterization of proteins and enzymes isolated from organisms living under unusual conditions |
Expression, purification and partial characterization of the Krüppel-associated box (KRAB) from the human ZNF2 protein
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View Export to BibTeX Export to EndNote
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View Export to BibTeX Export to EndNote
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View Export to BibTeX Export to EndNote
Palmieri M, Russo L, Malgieri G, Esposito S, Baglivo I, Rivellino A, Farina B, de Paola I, Zaccaro L, Milardi D, Isernia C, Pedone PV, Fattorusso R
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J Chem Res (ISSN: 0162-0134, 1873-3344, 0162-0134print), 2014 Feb; 131: 30-36.
Impact Factor: 3.444
View Export to BibTeX Export to EndNote
Malgieri G, Palmieri M, Esposito S, Maione V, Russo L, Baglivo I, De Paola I, Milardi D, Diana D, Zaccaro L, Pedone PV, Fattorusso R, Isernia C
* Zinc To Cadmium Replacement In The Prokaryotic Zinc-Finger Domain (458 views)
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Impact Factor: 3.978
View Export to BibTeX Export to EndNote
Palmieri M, Malgieri G, Russo L, Baglivo I, Esposito S, Netti F, Del Gatto A, De Paola I, Zaccaro L, Pedone PV, Isernia C, Milardi D, Fattorusso R
* Structural Zn(II) Implies a Switch from Fully Cooperative to Partly Downhill Folding in Highly Homologous Proteins (408 views)
J Am Chem Soc (ISSN: 0002-7863, 0002-2786, 1520-5126), 2013 Apr 2; 135(13): 5220-5228.
Impact Factor: 11.444
View Export to BibTeX Export to EndNote
Avitabile C, Moggio L, Malgieri G, Capasso D, Di Gaetano S, Saviano M, Pedone C, Romanelli A
* Gamma Sulphate Pna (pna S): Highly Selective Dna Binding Molecule Showing Promising Antigene Activity (451 views)
Plosone (ISSN: 1932-6203, 1932-6203electronic, 1932-6203linking), 2012 May 7; 7(5): e35774-e35774.
Impact Factor: 3.73
View Export to BibTeX Export to EndNote
Malgieri G, Zaccaro L, Leone M, Bucci E, Esposito S, Baglivo I, Del Gatto A, Russo L, Scandurra R, Pedone PV, Fattorusso R, Isernia C
* Zinc to Cadmium Replacement in the A. thaliana SUPERMAN Cys(2)His(2) Zinc Finger Induces Structural Rearrangements of Typical DNA Base Determinant Positions (445 views)
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Impact Factor: 2.87
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Malgieri G, Russo L, Esposito S, Baglivo I, Zaccaro L, Pedone EM, Di Blasio B, Isernia C, Pedone PV, Fattorusso R
* The Prokaryotic Cys2his2 Zinc Finger Domain Adopts A Novel Fold As Revealed By The Solution Structure Of Agrobacterium Tumefaciens Ros Dna Binding Domain (480 views)
P Natl Acad Sci Usa (ISSN: 0027-8424, 1091-6490, 0027-8424print), 2007 Oct 30; 104(44): 17341-17346.
Impact Factor: 9.598
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Esposito S, Baglivo I, Malgieri G, Russo L, Zaccaro L, D'Andrea LD, Mammucari M, Di Blasio B, Isernia C, Fattorusso R, Pedone PV
* A novel type of zinc finger DNA binding domain in the Agrobacterium tumefaciens transcriptional regulator Ros (724 views)
Biochemistry (ISSN: 0006-2960, 1520-4995, 1520-4995electronic), 2006 Sep 29; 45(34): 10394-10405.
Impact Factor: 3.633
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Mannini R, Rivieccio V, D'Auria S, Tanfani F, Ausili A, Pedone C, Grimaldi G, Facchiano A
* Structure/function of KRAB repression domains: Structural properties of KRAB modules inferred from hydrodynamic, circular dichroism, and FTIR spectroscopic analyses (691 views)
Proteins (ISSN: 0887-3585, 1097-0134, 1097-0134electronic), 2006 Feb 15; 62(3): 604-616.
Impact Factor: 3.73
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Saviano M, Isernia C, Bassarello C, Di Lello P, Galdiero S, Mierke DF, Benedetti E, Pedone C
* Conformational analysis by NMR and distance geometry techniques of a peptide mimetic of the third helix of the Antennapedia homeodomain (473 views)
J Pept Res (ISSN: 1397-002x), 2005 Feb; 65(2): 200-208.
Impact Factor: 1.741
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Isernia C, Bucci E, Leone M, Zaccaro L, Di Lello P, Digilio G, Esposito S, Saviano M, Di Blasio B, Pedone C, Pedone PV, Fattorusso R
* NMR structure of the single QALGGH zinc finger domain from the Arabidopsis thaliana SUPERMAN protein (430 views)
Chembiochem (ISSN: 1439-4227, 1439-7633, 1439-7633electronic), 2003 Mar 3; 4(2-3): 171-180.
Impact Factor: 3.992
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Dathan N, Zaccaro L, Esposito S, Isernia C, Omichinski JG, Riccio A, Pedone C, Di Blasio B, Fattorusso R, Pedone PV
* The Arabidopsis SUPERMAN protein is able to specifically bind DNA through its single Cys(2)-His(2) zinc finger motif (693 views)
Nucleic Acids Res (ISSN: 0305-1048, 1362-4962, 1362-4962electronic), 2002 Nov 15; 30(22): 4945-4951.
Impact Factor: 7.051
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Saviano M, Romanelli A, Bucci E, Pedone C, Mischiati C, Bianchi N, Feriotto G, Borgatti M, Gambari R
Computational procedures to explain the different biological activity of DNA/DNA, DNA/PNA and PNA/PNA hybrid molecules mimicking NF-κB binding sites (493 views)
J Biomol Struct Dyn (ISSN: 0739-1102, 1538-0254electronic, 0739-1102linking), 2000 Dec; 18(3): 353-362.
Impact Factor: 1.826
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18 Records (17 excluding Abstracts).
Total impact factor: 82.398 (78.668 excluding Abstracts).
Total 5 year impact factor: 81.19 (76.946 excluding Abstracts).
Total impact factor: 82.398 (78.668 excluding Abstracts).
Total 5 year impact factor: 81.19 (76.946 excluding Abstracts).
362 views. Last view on Monday 22 May 2023, 1:27:29
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