Expression, purification and partial characterization of the Krüppel-associated box (KRAB) from the human ZNF2 protein (751 views)
Protein Pept Lett (ISSN: 0929-8665, 1875-5305), 2005; 12(6): 527-532.
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Paper type: Journal Article,
Impact factor: 0.84, 5-year impact factor: 1.493
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-23644442274&partnerID=40&md5=99d6624f6b29f3f0245d9c0b0fabda65
Keywords: Fusion Protein Expression, Krab Domain, Transcriptional Repression, Zinc Finger, Dna Binding Protein, Recombinant Protein, Znf2 Protein, Human, Amino Acid Sequence, Article, Biosynthesis, Chemistry, Dna Sequence, Escherichia Coli, Gel Chromatography, Gene Expression, Genetics, High Performance Liquid Chromatography, Isolation And Purification, Molecular Genetics, Molecular Weight, Polyacrylamide Gel Electrophoresis, Protein Tertiary Structure, Sequence Alignment, High Pressure Liquid, Dna-Binding Proteins, Molecular Sequence Data, Protein Structure, Sequence Analysis,
Affiliations:
*** IBB - CNR ***
Istituto di Genetica e Biofisica A. Buzzati Traverso, Consiglio Nazionale Delle Ricerche, Italy
Istituto di Biochimica Delle Proteine, Consiglio Nazionale Delle Ricerche, Italy
Istituto di Biostrutture e Bioimmagini, Consiglio Nazionale Delle Ricerche, Italy
Dipartimento di Biochimica e Biofisica F. Cedrangolo, Seconda Università di Napoli, Italy
References:
Not available.
Protein Pept Lett (ISSN: 0929-8665, 1875-5305), 2005; 12(6): 527-532.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 0.84, 5-year impact factor: 1.493
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-23644442274&partnerID=40&md5=99d6624f6b29f3f0245d9c0b0fabda65
Keywords: Fusion Protein Expression, Krab Domain, Transcriptional Repression, Zinc Finger, Dna Binding Protein, Recombinant Protein, Znf2 Protein, Human, Amino Acid Sequence, Article, Biosynthesis, Chemistry, Dna Sequence, Escherichia Coli, Gel Chromatography, Gene Expression, Genetics, High Performance Liquid Chromatography, Isolation And Purification, Molecular Genetics, Molecular Weight, Polyacrylamide Gel Electrophoresis, Protein Tertiary Structure, Sequence Alignment, High Pressure Liquid, Dna-Binding Proteins, Molecular Sequence Data, Protein Structure, Sequence Analysis,
Affiliations:
*** IBB - CNR ***
Istituto di Genetica e Biofisica A. Buzzati Traverso, Consiglio Nazionale Delle Ricerche, Italy
Istituto di Biochimica Delle Proteine, Consiglio Nazionale Delle Ricerche, Italy
Istituto di Biostrutture e Bioimmagini, Consiglio Nazionale Delle Ricerche, Italy
Dipartimento di Biochimica e Biofisica F. Cedrangolo, Seconda Università di Napoli, Italy
References:
Not available.
Expression, purification and partial characterization of the Krüppel-associated box (KRAB) from the human ZNF2 protein
The Krüppel-associated box (KRAB) domain is a potent transcription repression bipartite domain, shared by over 400 zinc finger proteins in humans, involved in the regulation of many functions. KRAB domains are both physically and functionally bipartite (A and B modules). The lone KRAB-A and composite KRAB-AB domains from the human ZNF2 protein were over-expressed as recombinant proteins in E. coli, isolated and purified to homogeneity to investigate their structure to function relationship. © 2005 Bentham Science Publishers Ltd.
The Krüppel-associated box (KRAB) domain is a potent transcription repression bipartite domain, shared by over 400 zinc finger proteins in humans, involved in the regulation of many functions. KRAB domains are both physically and functionally bipartite (A and B modules). The lone KRAB-A and composite KRAB-AB domains from the human ZNF2 protein were over-expressed as recombinant proteins in E. coli, isolated and purified to homogeneity to investigate their structure to function relationship. © 2005 Bentham Science Publishers Ltd.
Expression, purification and partial characterization of the Krüppel-associated box (KRAB) from the human ZNF2 protein
| ▼ Structural characterization of proteins and enzymes isolated from organisms living under unusual conditions |
Expression, purification and partial characterization of the Krüppel-associated box (KRAB) from the human ZNF2 protein
Other filter: ([btitle,keywords] "Fusion Protein Expre ...
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Malgieri G, Zaccaro L, Leone M, Bucci E, Esposito S, Baglivo I, Del Gatto A, Russo L, Scandurra R, Pedone PV, Fattorusso R, Isernia C
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Malgieri G, Russo L, Esposito S, Baglivo I, Zaccaro L, Pedone EM, Di Blasio B, Isernia C, Pedone PV, Fattorusso R
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Esposito S, Baglivo I, Malgieri G, Russo L, Zaccaro L, D'Andrea LD, Mammucari M, Di Blasio B, Isernia C, Fattorusso R, Pedone PV
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Mannini R, Rivieccio V, D'Auria S, Tanfani F, Ausili A, Pedone C, Grimaldi G, Facchiano A
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* Exploring the Interaction between the SWI/SNF Chromatin Remodeling Complex and the Zinc Finger Factor CTCF (351 views)
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Impact Factor: 4.556
View Export to BibTeX Export to EndNote
D'Abrosca G, Paladino A, Baglivo I, Russo L, Sassano M, Grazioso R, Iacovino R, Pirone L, Pedone E, Pedone P, Isernia C, Fattorusso R, Malgieri G
* Structural Insight of the Full-Length Ros Protein: A Prototype of the Prokaryotic Zinc-Finger Family (659 views) (PDF 77 views)
Sci Rep (ISSN: 2045-2322, 2045-2322linking, 2045-2322electronic), 2020 Jun 9; 10(1): 9283-9283.
Impact Factor: 4.011
View Export to BibTeX Export to EndNote
Pirone L, Pitzer JE, D'Abrosca G, Fattorusso R, Malgieri G, Pedone EM, Pedone PV, Roop RM, Baglivo I
* Identifying the region responsible for Brucella abortus MucR higher-order oligomer formation and examining its role in gene regulation (475 views)
Sci Rep (ISSN: 2045-2322electronic, 2045-2322linking), 2018 Nov 22; 8(1): 17238-17238.
Impact Factor: 5.078
View Export to BibTeX Export to EndNote
D'Abrosca G, Russo L, Palmieri M, Baglivo I, Netti F, De Paola I, Zaccaro L, Farina B, Iacovino R, Pedone PV, Isernia C, Fattorusso R, Malgieri G
* The (unusual) aspartic acid in the metal coordination sphere of the prokaryotic zinc finger domain (577 views)
J Chem Res (ISSN: 0162-0134, 1873-3344, 0162-0134print), 2016 Aug; 161: 91-98.
Impact Factor: 3.348
View Export to BibTeX Export to EndNote
Russo L, Palmieri M, Caso JV, Abrosca GD, Diana D, Malgieri G, Baglivo I, Isernia C, Pedone PV, Fattorusso R
* Towards understanding the molecular recognition process in prokaryotic zinc-finger domain (1303 views)
Eur J Med Chem (ISSN: 0223-5234, 1768-3254, 0223-5234linking), 2015; 91: 100-108.
Impact Factor: 3.902
View Export to BibTeX Export to EndNote
Antoniali G, Lirussi L, D'Ambrosio C, Dal Piaz F, Vascotto C, Casarano E, Marasco D, Scaloni A, Fogolari F, Tell G
* SIRT1 gene expression upon genotoxic damage is regulated by APE1 through nCaRE-promoter elements (888 views)
Mol Biol Cell (ISSN: 1059-1524), 2014 Feb 15; 25(4): 532-547.
Impact Factor: 4.466
View Export to BibTeX Export to EndNote
Palmieri M, Russo L, Malgieri G, Esposito S, Baglivo I, Rivellino A, Farina B, de Paola I, Zaccaro L, Milardi D, Isernia C, Pedone PV, Fattorusso R
* Deciphering the zinc coordination properties of the prokaryotic zinc finger domain: The solution structure characterization of Ros87 H42A functional mutant (748 views)
J Chem Res (ISSN: 0162-0134, 1873-3344, 0162-0134print), 2014 Feb; 131: 30-36.
Impact Factor: 3.444
View Export to BibTeX Export to EndNote
Malgieri G, Palmieri M, Esposito S, Maione V, Russo L, Baglivo I, De Paola I, Milardi D, Diana D, Zaccaro L, Pedone PV, Fattorusso R, Isernia C
* Zinc To Cadmium Replacement In The Prokaryotic Zinc-Finger Domain (888 views)
Metallomics (ISSN: 1756-591x, 1756-5901, 1756-591xelectronic), 2013 Dec 18; 6(1): 96-104.
Impact Factor: 3.978
View Export to BibTeX Export to EndNote
Palmieri M, Malgieri G, Russo L, Baglivo I, Esposito S, Netti F, Del Gatto A, De Paola I, Zaccaro L, Pedone PV, Isernia C, Milardi D, Fattorusso R
* Structural Zn(II) Implies a Switch from Fully Cooperative to Partly Downhill Folding in Highly Homologous Proteins (609 views)
J Am Chem Soc (ISSN: 0002-7863, 0002-2786, 1520-5126), 2013 Apr 2; 135(13): 5220-5228.
Impact Factor: 11.444
View Export to BibTeX Export to EndNote
Avitabile C, Moggio L, Malgieri G, Capasso D, Di Gaetano S, Saviano M, Pedone C, Romanelli A
* Gamma Sulphate Pna (pna S): Highly Selective Dna Binding Molecule Showing Promising Antigene Activity (882 views)
Plosone (ISSN: 1932-6203, 1932-6203electronic, 1932-6203linking), 2012 May 7; 7(5): e35774-e35774.
Impact Factor: 3.73
View Export to BibTeX Export to EndNote
Malgieri G, Zaccaro L, Leone M, Bucci E, Esposito S, Baglivo I, Del Gatto A, Russo L, Scandurra R, Pedone PV, Fattorusso R, Isernia C
* Zinc to Cadmium Replacement in the A. thaliana SUPERMAN Cys(2)His(2) Zinc Finger Induces Structural Rearrangements of Typical DNA Base Determinant Positions (836 views)
Biopolymers (ISSN: 0006-3525, 0006-6352, 0006-3525print), 2011 Nov; 95(11): 801-810.
Impact Factor: 2.87
View Export to BibTeX Export to EndNote
Malgieri G, Russo L, Esposito S, Baglivo I, Zaccaro L, Pedone EM, Di Blasio B, Isernia C, Pedone PV, Fattorusso R
* The Prokaryotic Cys2his2 Zinc Finger Domain Adopts A Novel Fold As Revealed By The Solution Structure Of Agrobacterium Tumefaciens Ros Dna Binding Domain (921 views)
P Natl Acad Sci Usa (ISSN: 0027-8424, 1091-6490, 0027-8424print), 2007 Oct 30; 104(44): 17341-17346.
Impact Factor: 9.598
View Export to BibTeX Export to EndNote
Esposito S, Baglivo I, Malgieri G, Russo L, Zaccaro L, D'Andrea LD, Mammucari M, Di Blasio B, Isernia C, Fattorusso R, Pedone PV
* A novel type of zinc finger DNA binding domain in the Agrobacterium tumefaciens transcriptional regulator Ros (1498 views)
Biochemistry (ISSN: 0006-2960, 1520-4995, 1520-4995electronic), 2006 Sep 29; 45(34): 10394-10405.
Impact Factor: 3.633
View Export to BibTeX Export to EndNote
Mannini R, Rivieccio V, D'Auria S, Tanfani F, Ausili A, Pedone C, Grimaldi G, Facchiano A
* Structure/function of KRAB repression domains: Structural properties of KRAB modules inferred from hydrodynamic, circular dichroism, and FTIR spectroscopic analyses (1198 views)
Proteins (ISSN: 0887-3585, 1097-0134, 1097-0134electronic), 2006 Feb 15; 62(3): 604-616.
Impact Factor: 3.73
View Export to BibTeX Export to EndNote
Saviano M, Isernia C, Bassarello C, Di Lello P, Galdiero S, Mierke DF, Benedetti E, Pedone C
* Conformational analysis by NMR and distance geometry techniques of a peptide mimetic of the third helix of the Antennapedia homeodomain (847 views)
J Pept Res (ISSN: 1397-002x), 2005 Feb; 65(2): 200-208.
Impact Factor: 1.741
View Export to BibTeX Export to EndNote
Isernia C, Bucci E, Leone M, Zaccaro L, Di Lello P, Digilio G, Esposito S, Saviano M, Di Blasio B, Pedone C, Pedone PV, Fattorusso R
* NMR structure of the single QALGGH zinc finger domain from the Arabidopsis thaliana SUPERMAN protein (772 views)
Chembiochem (ISSN: 1439-4227, 1439-7633, 1439-7633electronic), 2003 Mar 3; 4(2-3): 171-180.
Impact Factor: 3.992
View Export to BibTeX Export to EndNote
Dathan N, Zaccaro L, Esposito S, Isernia C, Omichinski JG, Riccio A, Pedone C, Di Blasio B, Fattorusso R, Pedone PV
* The Arabidopsis SUPERMAN protein is able to specifically bind DNA through its single Cys(2)-His(2) zinc finger motif (1624 views)
Nucleic Acids Res (ISSN: 0305-1048, 1362-4962, 1362-4962electronic), 2002 Nov 15; 30(22): 4945-4951.
Impact Factor: 7.051
View Export to BibTeX Export to EndNote
Saviano M, Romanelli A, Bucci E, Pedone C, Mischiati C, Bianchi N, Feriotto G, Borgatti M, Gambari R
Computational procedures to explain the different biological activity of DNA/DNA, DNA/PNA and PNA/PNA hybrid molecules mimicking NF-κB binding sites (876 views)
J Biomol Struct Dyn (ISSN: 0739-1102, 1538-0254electronic, 0739-1102linking), 2000 Dec; 18(3): 353-362.
Impact Factor: 1.826
View Export to BibTeX Export to EndNote
18 Records (17 excluding Abstracts).
Total impact factor: 82.398 (78.668 excluding Abstracts).
Total 5 year impact factor: 81.19 (76.946 excluding Abstracts).
Total impact factor: 82.398 (78.668 excluding Abstracts).
Total 5 year impact factor: 81.19 (76.946 excluding Abstracts).
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