A Small Synthetic Cripto Blocking Peptide Improves Neural Induction, Dopaminergic Differentiation, and Functional Integration of Mouse Embryonic Stem Cells in a Rat Model of Parkinson's Disease (864 views)
Stem Cells (ISSN: 1066-5099), 2010 Sep; 28(8): 1326-1337.
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Paper type: Journal Article,
Impact factor: 7.871, 5-year impact factor: 8.632
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-77955786933&partnerID=40&md5=06fc54182f3d8174c573226542359b98
Keywords: Cell Transplantation, Cellular Therapy, Embryonic Stem Cells, Neural Differentiation, Parkinson, S Disease, Activin Receptor 1, Glycosylphosphatidylinositol, Peptide, Peptide Library, Tetramer, Tripeptide, Animal Cell, Animal Experiment, Animal Model, Animal Tissue, Article, Carcinogenesis, Cell Differentiation, Combinatorial Library, Controlled Study, Convalescence, Corpus Striatum, Dopaminergic Nerve Cell, Embryo Cell, Engraftment, Genetic Manipulation, In Vitro Study, In Vivo Study, Integration, Mesencephalon, Mouse, Mouse Embryo, Nerve Cell Differentiation, Neuroectoderm, Nonhuman, Parkinson Disease, Signal Transduction, Type I, Disease Models, Epidermal Growth Factor, Membrane Glycoproteins, Neoplasm Proteins, Oligopeptides, Protein Binding, Reverse Transcriptase Polymerase Chain Reaction, Stem Cell Transplantation, Rattus,
Affiliations:
*** IBB - CNR ***
Stem Cell Fate Laboratory, Institute of Genetics and Biophysics, A. Buzzati-Traverso, CNR, Via Pietro Castellino 111, 80,131 Naples, Italy
Laboratory of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
Florey Neuroscience Institutes, University of Melbourne, Parkville, VIC, Australia
Centre for Neurosciences, University of Melbourne, Parkville, VIC, Australia
Department of Biological Science, University of Naples, Napoli, Italy
Institute of Biostructure and Bioimaging, CNR, Naples, Italy
References:
Not available.
Stem Cells (ISSN: 1066-5099), 2010 Sep; 28(8): 1326-1337.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 7.871, 5-year impact factor: 8.632
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-77955786933&partnerID=40&md5=06fc54182f3d8174c573226542359b98
Keywords: Cell Transplantation, Cellular Therapy, Embryonic Stem Cells, Neural Differentiation, Parkinson, S Disease, Activin Receptor 1, Glycosylphosphatidylinositol, Peptide, Peptide Library, Tetramer, Tripeptide, Animal Cell, Animal Experiment, Animal Model, Animal Tissue, Article, Carcinogenesis, Cell Differentiation, Combinatorial Library, Controlled Study, Convalescence, Corpus Striatum, Dopaminergic Nerve Cell, Embryo Cell, Engraftment, Genetic Manipulation, In Vitro Study, In Vivo Study, Integration, Mesencephalon, Mouse, Mouse Embryo, Nerve Cell Differentiation, Neuroectoderm, Nonhuman, Parkinson Disease, Signal Transduction, Type I, Disease Models, Epidermal Growth Factor, Membrane Glycoproteins, Neoplasm Proteins, Oligopeptides, Protein Binding, Reverse Transcriptase Polymerase Chain Reaction, Stem Cell Transplantation, Rattus,
Affiliations:
*** IBB - CNR ***
Stem Cell Fate Laboratory, Institute of Genetics and Biophysics, A. Buzzati-Traverso, CNR, Via Pietro Castellino 111, 80,131 Naples, Italy
Laboratory of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
Florey Neuroscience Institutes, University of Melbourne, Parkville, VIC, Australia
Centre for Neurosciences, University of Melbourne, Parkville, VIC, Australia
Department of Biological Science, University of Naples, Napoli, Italy
Institute of Biostructure and Bioimaging, CNR, Naples, Italy
References:
Not available.
A Small Synthetic Cripto Blocking Peptide Improves Neural Induction, Dopaminergic Differentiation, and Functional Integration of Mouse Embryonic Stem Cells in a Rat Model of Parkinson's Disease
Cripto is a glycosylphosphatidylinositol-anchored coreceptor that binds Nodal and the activin type I (ALK) -4 receptor, and is involved in cardiac differentiation of mouse embryonic stem cells (mESCs). Interestingly, genetic ablation of cripto results in increased neuralization and midbrain dopaminergic (DA) differentiation of mESCs, as well as improved DA cell replacement therapy (CRT) in a model of Parkinson's disease (PD). In this study, we developed a Cripto specific blocking tool that would mimic the deletion of cripto, but could be easily applied to embryonic stem cell (ESC) lines without the need of genetic manipulation. We thus screened a combinatorial peptide library and identified a tetrameric tripeptide, Cripto blocking peptide (BP), which prevents Cripto/ALK-4 receptor interaction and interferes with Cripto signaling. Cripto BP treatment favored neuroectoderm formation and promoted midbrain DA neuron differentiation of mESCs in vitro and in vivo. Remarkably, Cripto BP-treated ESCs, when transplanted into the striatum of PD rats, enhanced functional recovery and reduced tumor formation, mimicking the effect of genetic ablation of cripto. We therefore suggest that specific blockers such as Cripto BP may be used to improve the differentiation of ESC-derived DA neurons in vitro and their engraftment in vivo, bringing us closer towards an application of ESCs in CRT. STEM CELLS 2010; 28: 1326-1337
Cripto is a glycosylphosphatidylinositol-anchored coreceptor that binds Nodal and the activin type I (ALK) -4 receptor, and is involved in cardiac differentiation of mouse embryonic stem cells (mESCs). Interestingly, genetic ablation of cripto results in increased neuralization and midbrain dopaminergic (DA) differentiation of mESCs, as well as improved DA cell replacement therapy (CRT) in a model of Parkinson's disease (PD). In this study, we developed a Cripto specific blocking tool that would mimic the deletion of cripto, but could be easily applied to embryonic stem cell (ESC) lines without the need of genetic manipulation. We thus screened a combinatorial peptide library and identified a tetrameric tripeptide, Cripto blocking peptide (BP), which prevents Cripto/ALK-4 receptor interaction and interferes with Cripto signaling. Cripto BP treatment favored neuroectoderm formation and promoted midbrain DA neuron differentiation of mESCs in vitro and in vivo. Remarkably, Cripto BP-treated ESCs, when transplanted into the striatum of PD rats, enhanced functional recovery and reduced tumor formation, mimicking the effect of genetic ablation of cripto. We therefore suggest that specific blockers such as Cripto BP may be used to improve the differentiation of ESC-derived DA neurons in vitro and their engraftment in vivo, bringing us closer towards an application of ESCs in CRT. STEM CELLS 2010; 28: 1326-1337
A Small Synthetic Cripto Blocking Peptide Improves Neural Induction, Dopaminergic Differentiation, and Functional Integration of Mouse Embryonic Stem Cells in a Rat Model of Parkinson's Disease
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A Small Synthetic Cripto Blocking Peptide Improves Neural Induction, Dopaminergic Differentiation, and Functional Integration of Mouse Embryonic Stem Cells in a Rat Model of Parkinson's Disease
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10 Records (9 excluding Abstracts).
Total impact factor: 73.963 (64.62 excluding Abstracts).
Total 5 year impact factor: 72.372 (62.751 excluding Abstracts).
Total impact factor: 73.963 (64.62 excluding Abstracts).
Total 5 year impact factor: 72.372 (62.751 excluding Abstracts).
864 views. Last view on Sunday 06 October 2024, 20:40:19
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